30 results on '"Moran Jr, Charles P."'
Search Results
2. MACROMOLECULAR SYNTHESIS: 21 RNA Polymerase and Sigma Factors.
- Author
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HELMANN, JOHN D. and MORAN JR., CHARLES P.
- Published
- 2002
3. A LytM Domain Dictates the Localization of Proteins to the Mother Cell-Forespore Interface during Bacterial Endospore Formation.
- Author
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Meisner, Jeffrey and Moran Jr., Charles P.
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PROTEINS , *MOLECULES , *BACTERIAL spores , *BACILLUS subtilis , *SPORANGIUM , *CALORIMETRY , *MEMBRANE proteins - Abstract
A large number of proteins are known to reside at specific subcellular locations in bacterial cells. However, the molecular mechanisms by which many of these proteins are anchored at these locations remains unclear. During endospore formation in Bacillus subtilis, several integral membrane proteins are located specifically at the interface of the two adjacent cells of the developing sporangium, the mother cell and forespore. The mother cell membrane protein SpoIIIAH recognizes the cell-cell interface through an interaction with the forespore membrane protein SpolIQ, and then the other proteins are positioned there by the SpoIIIAH-SpoIIQ complex. In this study, we investigated the molecular mechanisms underlying the formation of the SpoIIIAH-SpoIIQ complex. Using gel filtration chromatography and isothermal titration calorimetry, we measured the binding parameters that characterize the SpoIIIAH-SpoIIQ interaction in vitro. We also demonstrated that the interaction of SpoIIIAH and SpoIIQ is governed by their YscJ and degenerate LytM domains, respectively. Therefore, the LytM domain of SpoIIQ provides the positional cue that dictates the localization of mother cell membrane proteins to the mother cell-forespore interface. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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4. VII. TRANSCRIPTION AND TRANSLATION MACHINERY: 45. RNA Polymerase and Transcription Factors.
- Author
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MORAN JR., CHARLES P.
- Published
- 1993
5. Two Regions of GerE Required for Promoter Activation in Bacillus subtilis.
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Crater, Dinene L. and Moran Jr., Charles P.
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BACILLUS subtilis , *PROMOTERS (Genetics) , *ALANINE - Abstract
Investigates the region of GerE required for promoter activation in bacillus subtilis. Description of GerE; Effects of single alanine substitutions on transcription; Classification of the transcriptional activators in bacteria.
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- 2002
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6. Identification of a DNA Region in GerE from Bacillus subtilis.
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Crater, Dinene L. and Moran Jr., Charles P.
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PROTEIN binding , *BACILLUS subtilis , *BACTERIAL genetics , *PROTEIN metabolism - Abstract
Examines the effects of single base pair substitutions in the high affinity GerE protein binding site on the sigK promoter in Bacillus subtilis. Production of a protein that binds with equal affinity to two sites that differ by one base pair; Identification of the nucleotide sequence that signals recognition and binding by GerE; Mutagenesis of the DNA binding region of GerE.
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- 2001
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7. A region in Bacillus subtilis ...H required for SpoOA-dependent promoter activity.
- Author
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Buckner, Cindy M. and Moran Jr., Charles P.
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ALANINE , *BACILLUS subtilis - Abstract
Cites a study which examined the effects of single alanine substitutions, in an attempt to determine SpoOA activities in the bacterium Bacillus subtilis. What SpoOA is; Isolation of the derivatives of sigH mutants of SpoOA; Results of the study.
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- 1998
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8. SigmaE changed to sigmaB specificity by amino acid substitutions in its -10 binding region.
- Author
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Tatti, Kathleen M. and Moran Jr, Charles P. .
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BACTERIAL genetics , *RNA polymerases - Abstract
Tests whether base pair substitutions in the -10 region of a sigmaB-dependent promoter could signal its utilization by sigmaE-RNA polymerase in bacteria. Changes in specificity upon amino acid substitutions in sigmaE; Mutagenesis and expression of sigE.
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- 1995
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9. Cloning and characterization of spoVR, a gene from Bacillus subtilis involved in spore cortex...
- Author
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Beall, Bernard and Moran Jr, Charles P. .
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BACILLUS subtilis genetics , *BACTERIAL spores - Abstract
Describes the cloning and characterization of spoVR, a gene from Bacillus subtilis involved in spore cortex formation. Increase in the proportion of phase-dark spores; Decrease in the accumulation of dipicolinate; Initiation of the expression of spoVR.
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- 1994
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10. Autoregulation of SafA Assembly through Recruitment of a Protein Cross-Linking Enzyme.
- Author
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Fernandes, Catarina G., Moran Jr., Charles P., and Henriques, Adriano O.
- Abstract
The coat of Bacillus subtilis spores is a multiprotein protective structure that also arbitrates many of the environmental interactions of the spore. The coat assembles around the cortex peptidoglycan layer and is differentiated into an inner and an outer layer and a crust. SafA governs assembly of the inner coat, whereas CotE drives outer coat assembly. SafA localizes to the cortex-coat interface. Both SafA and its short form C30 are substrates for Tgl, a coat-associated transglutaminase that cross-links proteins through ε-(γ-glutamyl)lysyl isopeptide bonds. We show that SafA and C30 are distributed between the coat and cortex layers. The deletion of tgl increases the extractability of SafA, mainly from the cortex. Tgl itself is mostly located in the inner coat and cortex. The localization of Tgl-cyan fluorescent protein (Tgl-CFP) is strongly, but not exclusively, dependent on safA. However, the association of Tgl with the cortex requires safA. Together, our results suggest an assembly pathway in which Tgl is first recruited to the forming spore in a manner that is only partially dependent on SafA and then is drafted to the cortex by SafA. Tgl, in turn, promotes the conversion of coat- and cortex-associated SafA into forms that resist extraction, possibly by catalyzing the cross-linking of SafA to other coat proteins, to the cortex, and/or to cortex-associated proteins. Therefore, the final assembly state of SafA relies on an autoregulatory pathway that requires the subcellular localization of a protein cross-linking enzyme. Tgl most likely exerts a "spotwelding" activity, cross-linking preformed complexes in the cortex and inner coat layers of spores. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
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11. Promoter Activation by Repositioning of RNA Polymerase.
- Author
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Kumar, Amrita and Moran Jr., Charles P.
- Subjects
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RNA polymerases , *NUCLEOTIDE sequence , *BACILLUS subtilis , *BACILLUS (Bacteria) , *DNA , *NUCLEIC acid analysis - Abstract
Spo0A, a classical two-component-type response regulator in Bacillus subtilis, binds to a specific DNA sequence found in many promoters to repress or activate the transcription of over 100 genes. On the spoIIG promoter, one of the Spo0A binding sites, centered at position 40, overlaps a consensus 35 element that may also interact with region 4 of the sigma A (σA) subunit of RNA polymerase. Molecular modeling corroborated by genetic evidence led us to propose that the binding of Spo0A to this site repositions σA region 4 on the promoter. Therefore, we used a chemical nuclease, p-bromoacetamidobenzyl-EDTA-Fe, that was covalently tethered to a single cysteine in region 4 of σA to map the position of σA on the promoter. The results indicated that in the absence of Spo0A, σA region 4 of the RNA polymerase was located near the 35 element sequence centered at position 40. However, in the presence of Spo0A, σA region 4 was displaced downstream from the 35 element by 4 bp. These and other results support the model in which the binding of Spo0A to the spoIIG promoter stimulates promoter utilization by repositioning prebound RNA polymerase and stabilizing the repositioned RNA polymerase-promoter complex at a new position that aligns σA region 2 with the 10 region sequences of the promoter, thus facilitating open complex formation. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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12. Essential Internal Promoter in the spoIIIA Locus of Bacillus subtilis.
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Guillot, Chris and Moran Jr, Charles P.
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BACILLUS subtilis , *PROMOTERS (Genetics) , *GENE expression , *PHENOTYPES , *RNA polymerases , *PROTEINS - Abstract
The Bacillus subtilis spoIIIA locus encodes eight proteins, SpoIIIAA to SpoIIIAH, which are expressed in the mother cell during endospore formation and which are essential for the activation of σG in the forespore. Complementation studies indicated that this locus may be transcribed from two promoters, one promoter upstream from the first gene and possibly a second unidentified promoter within the locus. Fragments of the spoIIIA locus were expressed at an ectopic site to complement the sporulation-defective phenotype of a spoIIIAH deletion, and we determined that complementation required a fragment of DNA that extended into spoIIIAF. To confirm that there was a promoter located in spoIIIAF, we constructed transcriptional fusions to lacZ and found strong sporulation-induced promoter activity. Primer extension assays were used to determine the transcription start site, and point mutations introduced into the -10 and -35 regions of the promoter reduced its activity. This promoter is transcribed by σE-RNA polymerase and is repressed by SpoIIID. Therefore, we concluded that the spoIIIA locus is transcribed from two promoters, one at the start of the locus (P1spoIIIA) and the other within the locus (P2spoIIIA). Based on Campbell integrations and reverse transcription-PCR analysis of the P2spoIIIA region, we determined that P2spoIIIA is sufficient for transcription of spoIIIAG and spoIIIAH. Inactivation of P2spoIIIA blocked spore formation, indicating that P2spoIIIA is essential for expression of spoIIIAG and spoIIIAH. The P2spoIIIA activity is twice the P1spoIIIA activity; therefore, larger amounts of SpoIIIAG and SpoIIIAH than of proteins encoded at the upstream end of the locus may be required. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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13. Processing of a Membrane Protein Required for Cell-to-Cell Signaling during Endospore Formation in Bacillus subtilis.
- Author
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Serrano, Mónica, Vieira, Filipe, Moran Jr., Charles P., and Henriques, Adriano O.
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BACILLUS subtilis , *PROTEINS , *RNA polymerases , *PROTOPLASTS , *CYTOPLASM , *PEPTIDES - Abstract
Activation of the late prespore-specific RNA polymerase sigma factor σG during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of σG also requires expression of spoIIIJ, coding for a membrane protein translocase of the YidC/Oxa1p/Alb3 family, and of the mother cell-specific spoIIIA operon. Here we present genetic and biochemical evidence indicating that SpoIIIAE, the product of one of the spoIIIA cistrons, and SpoIIIJ interact in the membrane, thereby linking the function of the spoIIIJ and spoIIIA loci in the activation of σG. We also show that SpoIIIAE has a functional Sec-type signal peptide, which is cleaved during sporulation. Furthermore, mutations that reduce or eliminate processing of the SpoIIIAE signal peptide arrest sporulation following engulfment completion and prevent activation of σG. SpoIIIJ-type proteins can function in cooperation with or independently of the Sec system. In one model, SpoIIIJ interacts with SpoIIIAE in the context of the Sec translocon to promote its correct localization and/or topology in the membrane, so that it can signal the activation of σG following engulfment completion. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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14. α-Helix E of Spo0A Is Required for σA- but Not for σH-Dependent Promoter Activation in Bacillus subtilis.
- Author
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Kumar, Amrita, Brannigan, James A., and Moran Jr., Charles P.
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GENETIC regulation , *BACILLUS subtilis , *PROMOTERS (Genetics) , *GENETIC transcription , *BACILLUS (Bacteria) , *NUCLEOTIDES , *LIGASES - Abstract
The environmental strain Bacillus amyloliquefaciens FZB42 promotes plant growth and suppresses plant pathogenic organisms present in the rhizosphere. We sampled sequenced the genome of FZB42 and identified 2,947 genes with >50% identity on the amino acid level to the corresponding genes of Bacillus subtilis 168. Six large gene clusters encoding nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) occupied 7.5% of the whole genome. Two of the PKS and one of the NRPS encoding gene clusters were unique insertions in the FZB42 genome and are not present in B. subtilis 168. Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis revealed expression of the antibiotic lipopeptide products surfactin, fengycin, and bacillomycin D. The fengycin (fen) and the surfactin (srf) operons were organized and located as in B. subtilis 168. A large 37.2-kb antibiotic DNA island containing the bmy gene cluster was attributed to the biosynthesis of bacillomycin D. The bmy island was found inserted close to the fen operon. The responsibility of the bmy, fen, and srf gene clusters for the production of the corresponding secondary metabolites was demonstrated by cassette mutagenesis, which led to the loss of the ability to produce these peptides. Although these single mutants still largely retained their ability to control fungal spread, a double mutant lacking both bacillomycin D and fengycin was heavily impaired in its ability to inhibit growth of phytopathogenic fungi, suggesting that both lipopeptides act in a synergistic manner. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
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15. Expression of the Secondary Sigma Factor σ[sup X] in Streptococcus pyogenes Is Restricted at Two Levels.
- Author
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Opdyke, Jason A., Scott, June R., and Moran Jr., Charles P.
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PROTEINS , *STREPTOCOCCUS pyogenes , *RNA polymerases , *GENETIC transcription - Abstract
Secondary RNA polymerase sigma factors in many bacteria are responsible for regulating a vast range of processes including virulence. A protein (σ[sup x]) in the gram-positive human pathogen Streptococcus pyogenes (the group A Streptococcus or GAS) was recently shown to function in vitro as a secondary sigma factor. We report here the isolation of a mutant in which both sigX genes are inactivated, show that σ[sup x] functions in GAS cells, and show that the amount of σ[sup x] is controlled at two levels. Primer extension analysis indicates that sigX transcription is low in GAS cells grown in Todd-Hewitt yeast broth, and immunoblot assays with a σ[sup x]-specific polyclonal antibody demonstrate that the protein does not accumulate in these cells. To increase the level of sigX transcription in GAS, we constructed a strain that constitutively expresses the sigX gene from a heterologous promoter. Expression of sigX from this promoter led to transcription of the σ[sup x]-dependent cinA promoter in GAS cells. We found that expression of the sigX gene in a clpP mutant strain resulted in greater accumulation of σ[sup x] protein, which resulted in higher levels of transcription from the σ[sup x]-dependent promoters cinA, smf, and cglA. In addition, a clpP mutant containing sigX only at its wild-type loci on the chromosome generated more transcription from the σ[sup x]-dependent cinA promoter than did the wild-type parental strain. Therefore, σ[sup x] activity in GAS is limited by low-level transcription of the sigX structural genes and by clpP, which appears to negatively regulate σ[sup x] accumulation. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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16. Forespore-Specific Transcription of the lonB Gene during Sporulation in Bacillus subtilis.
- Author
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Serrano, Monica, Hovel, Sven, Moran Jr., Charles P., Henriques, Adriano O., and Volker, Uwe
- Subjects
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BACILLUS subtilis genetics , *GENETIC transcription - Abstract
Examines the forespore-specific transcription of the lonB gene during sporulation in Bacillus subtilis. Disruption of lonB gene; Dependence of lonB gene transcription on the sigF gene; Production of a monocistronic message.
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- 2001
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17. A region in the Bacillus subtilis transcription factor SpoOA that is important for spoIIG...
- Author
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Buckner, Cindy M., Schyns, Ghislain, and Moran Jr., Charles P.
- Subjects
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PROMOTERS (Genetics) , *DNA , *CARRIER proteins , *BACILLUS subtilis , *RNA - Abstract
Presents a study which examined the promoter activation of the spoIIG, with reference to the deoxyribonucleic acid (DNA) binding protein in Bacillus subtilis `SpoOA.' Interaction of the SpoOa; Details on ribonucleic acid (RNA) polymerase and transcriptional activators; Methodology used to conduct the study; Results of the study.
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- 1998
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18. Involvement of superoxide dismutase in spore coat assembly in Bacillus subtilis.
- Author
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Henriques, Adriano O., Melsen, Lawrence R., and Moran Jr., Charles P.
- Subjects
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BACILLUS subtilis - Abstract
Provides information on a study of the superoxide dismutase in spore coat assembly in Bacillus subtilis. Methodology and materials used to conduct the study; Results of the study.
- Published
- 1998
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19. Interaction between Coat Morphogenetic Proteins SafA and SpoVID.
- Author
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Costa, Teresa, Isidro, Anabela L., Moran Jr., Charles P., and Henriques, Adriano O.
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PROTEINS , *BACILLUS subtilis , *BACTERIAL spores , *LYSOZYMES , *PEPTIDOGLYCANS , *PEPTIDES - Abstract
Morphogenetic proteins such as SpoVID and SafA govern assembly of the Bacillus subtilis endospore coat by guiding the various protein structural components to the surface of the developing spore. Previously, a screen for peptides able to interact with SpoVID led to the identification of a PYYH motif present in the C-terminal half of the SafA protein and to the subsequent demonstration that SpoVID and SafA directly interact. spoVID and safA spores show deficiencies in coat assembly and are lysozyme susceptible. Both proteins, orthologs of which are found in all Bacillus species, have LysM domains for peptidoglycan binding and localize to the cortex-coat interface. Here, we show that the interaction between SafA and SpoVID involves the PYYH motif (region B) but also a 13-amino-acid region (region A) just downstream of the N-terminal LysM domain of SafA. We show that deletion of region B does not block the interaction of SafA with SpoVID, nor does it bring about spore susceptibility to lysozyme. Nevertheless, it appears to reduce the interaction and affects the complex. In contrast, lesions in region A impaired the interaction of SafA with SpoVID in vitro and, while not affecting the accumulation of SafA in vivo, interfered with the localization of SafA around the developing spore, causing aberrant assembly of the coat and lysozyme sensitivity. A peptide corresponding to region A interacts with SpoVID, suggesting that residues within this region directly contact SpoVID. Since region A is highly conserved among SafA orthologs, this motif may be an important determinant of coat assembly in the group of Bacillus spore formers. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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20. Assembly and Function of a Spore Coat-Associated Transglutaminase of Bacillus subtilis.
- Author
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Rita Zilhão, Isticato, Rachele, Martins, Lígia O., Steil, Leif, Völker, Uwe, Ricca, Ezio, Moran Jr., Charles P., and Henriques, Adriano O.
- Subjects
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BACILLUS subtilis , *TRANSGLUTAMINASES , *ENZYMES , *BACTERIAL spores , *AMINO acids , *PROTEINS - Abstract
The assembly of a multiprotein coat around the Bacillus subtilis spore confers resistance to lytic enzymes and noxious chemicals and ensures normal germination. Part of the coat is cross-linked and resistant to solubilization. The coat contains ε-(γ-glutamyl)lysyl cross-links, and the expression of the gene (tgl) for a sporee associated transglutaminase was shown before to be required for the cross-linking of coat protein GerQ. Here, we have investigated the assembly and function of Tgl. We found that Tgl associates, albeit at somewhat reduced levels, with the coats of mutants that are unable to assemble the outer coat (cotE), that are missing the inner coat and with a greatly altered outer coat (gerE), or that are lacking discernible inner and outer coat structures (cotE gerE double mutant). This suggests that Tgl is present at various levels within the coat lattice. The assembly of Tgl occurs independently of its own activity, as a single amino acid substitution of a cysteine to an alanine (C116A) at the active site of Tgl does not affect its accumulation or assembly. However, like a tgl insertional mutation, the tglC116A allele causes increased extractability of polypeptides of about 40, 28, and 16 kDa in addition to GerQ (20 kDa) and affects the structural integrity of the coat. We show that most Tgl is assembled onto the spore surface soon after its synthesis in the mother cell under &` control but that the complete insolubilization of at least two of the Tgl-controlled polypeptides occurs several hours later. We also show that a multicopy allele of tgl causes increased assembly of Tgl and affects the assembly, structure, and functional properties of the coat. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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21. A Gene Encoding a Holin-Like Protein Involved in Spore Morphogenesis and Spore Germination in Bacillus subtilis.
- Author
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Real, Gonçalo, Pinto, Sérgio M., Schyns, Ghislain, Costa, Teresa, Henriques, Adriano O., and Moran Jr., Charles P.
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GENETICS , *PROTEINS , *MORPHOGENESIS , *GERMINATION , *BACILLUS subtilis , *RNA polymerases , *BACTERIOLOGY - Abstract
We report here studies of expression and functional analysis of a Bacillus subtilis gene, ywcE, which codes tar a product with features of a holin. Primer extension analysis of ywcE transcription revealed that a single transcript accumulated from the onset of sporulation onwards, produced from a σA-type promoter bearing the TG dinucleotide motif of ‘extended’ -10 promoters. No primer extension product was detected in viva during growth. However, specific runoff products were produced in vitro from the ywcE promoter by purified σA-containing RNA polymerase (EσA), and the in viva and in vitro transcription start sites were identical. These results suggested that utilization of the ywcE promoter by EσA during growth was subjected to repression. Studies with a lacZ fusion revealed that the transition-state regulator AbrB repressed the transcription of ywcE during growth. This repression was reversed at the onset of sporulation in a Spo0A-dependent manner, but Spo0A did not appear to contribute otherwise to ywcE transcription. We found ywcE to be required for proper spore morphogenesis. Spores of the ywcE mutant showed a reduced outer coat which lacked the characteristic striated pattern, and the outer coat failed to attach to the underlying inner coat. The mutant spores also accumulated reduced levels of dipicolinic acid. ywcE was also found to be important for spore germination. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
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22. Role of the Anti-Sigma Factor SpoIIAB in Regulation of σG during Bacillus subtilis Sporulation.
- Author
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Serrano, Mónica, Neves, Alexandre, Soares, Cláudio M., Moran Jr., Charles P., and Henriques, Adriano O.
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BACILLUS subtilis , *BACILLUS (Bacteria) , *RNA polymerases , *TRANSFERASES , *GENE expression , *GENETIC regulation - Abstract
RNA polymerase sigma factor σF initiates the prespore-specific program of gene expression during Bacillus subtilis sporulation, σF governs transcription of spoIIIG, encoding the late prespore-specific regulator σG. However, transcription of spoIIIG is delayed relative to other genes under the control of σF, and after synthesis, erg is initially kept in an inactive form. Activation of erg requires the complete engulfment of the prespore by the mother cell and expression of the spoIIIA and spoIIIJ loci. We screened for random mutations in spoIIIG that bypassed the requirement for spoIIIA for the activation of σG. We found a mutation (spoIIIGE156K) that resulted in an amino acid substitution at position 156, which is adjacent to the position of a mutation (E155K) previously shown to prevent interaction of SpoIIAB with σG. Comparative modelling techniques and in vivo studies suggested that the spoIIIGE156K mutation interferes with the interaction of SpoIIAB with σG. The σGE156K isoform restored σG-directed gene expression to spoIIIA mutant cells. However, expression of sspE lacZ in the spollIA spoIIIGE156K double mutant was delayed relative to completion of the engulfment process and was not confined to the prespore. Rather, β-galactosidase accumulated throughout the entire cell at late times in development. This suggests that the activity of σGE156K is still regulated in the prespore of a spoIIIA mutant, but not by SpoIIAB. In agreement with this suggestion, we also found that expression of spoIIIGE156K from the promoter for the early prespore specific gene spoIIQ still resulted in sspE-lacZ induction at the normal time during sporulation, coincidentally with completion of the engulfment process. In contrast, transcription of spoIIIGE156K, but not of the wild-type spoIIIG gene, from the mother cell-specific spoIID promoter permitted the rapid induction of sspE-lacZ expression. Together, the results suggest that SpoIIAB is either redundant or has no role in the regulation of era in the prespore. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
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23. Interactions among CotB, CotG, and CotH during Assembly of the Bacillus subtilis Spore Coat.
- Author
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Zilhão, Rita, Serrano, Mónica, Isticato, Rachele, Ricca, Ezio, Moran Jr., Charles P., and Henriques, Adirano O.
- Subjects
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BACILLUS subtilis , *BACILLUS (Bacteria) , *BACTERIAL spores , *SPOREFORMING bacteria , *DNA , *GENES - Abstract
The development of genetic competence in Bacillus subtilis is regulated by a complex signal transduction cascade, which results in the synthesis of the competence transcription factor, encoded by comK. ComK is required for the transcription of the late competence genes that encode the DNA binding and uptake machinery and of genes required for homologous recombination. In vivo and in vitro experiments have shown that ComK is responsible for transcription activation at the comG promoter. In this study, we investigated the mechanism of this transcription activation. The intrinsic binding characteristics of RNA polymerase with and without ComK at the comG promoter were determined, demonstrating that ComK stabilizes the binding of RNA polymerase to the comG promoter. This stabilization probably occurs through interactions with the upstream DNA, since a deletion of the upstream DNA resulted in an almost complete abolishment of stabilization of RNA polymerase binding. Furthermore, a strong requirement for the presence of an extra AT box in addition to the common ComKbinding site was shown. In vitro transcription with B. subtilis RNA polymerase reconstituted with wild-type α-subunits and with C-terminal deletion mutants of the α-subunits was performed, demonstrating that these deletions do not abolish transcription activation by ComK. This indicates that ComK is not a type I activator. We also show that ComK is not required for open complex formation. A possible mechanism for transcription activation is proposed, implying that the major stimulatory effect of ComK is on binding of RNA polymerase. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
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24. Surfaces of Spo0A and RNA Polymerase Sigma Factor A That Interact at the SpoIIG Promoter in Bacillus subtilis.
- Author
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Kumar, Amrita, Starke, Cindy Buckner, DeZalia, Mark, and Moran Jr., Charles P.
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BACILLUS subtilis , *CARRIER proteins , *RNA polymerases , *AMINO acids , *BACTERIA , *BACTERIOLOGY - Abstract
In Bacillus subtilis, the DNA binding protein Spo0A activates transcription from two classes of promoters, those used by RNA polymerase containing the primary sigma factor, σ[sup A] (e.g., spoIIG), and those used by RNA polymerase containing the secondary sigma factor, σ[sup H] (e.g., spoIIA). Several single amino acid substitutions in region 4 of σA define positions in σ[sup A] that are specifically required for Spo0A-dependent promoter activation. Similarly, several single amino acid substitutions in Spo0A define positions in Spo0A that are required for σ[sup A]-dependent promoter activation but not for other functions of Spo0A. It is unknown whether these amino acids in Spo0A interact directly with those in region 4 of σ[sup A] or whether they interact with another subunit of RNA polymerase to effect promoter activation. Here we report the identification of a new amino acid in region 4 of σ[sup A], arginine at position 355 (R355), that is involved in Spo0A-dependent promoter activation. To further investigate the role of R355, we used the coordinates of Spo0A and sigma region 4, each in complex with DNA, to build a model for the interaction of σ[sup A] and Spo0A at the spoIIG promoter. We tested the model by examining the effects of amino acid substitutions in the putative interacting surfaces of these molecules. As predicted by the model, we found genetic evidence for interaction of R355 of σ[sup A] with glutamine at position 221 of Spo0A. These results appear to define the surfaces of Spo0A and σ[sup A] that directly interact during activation of the spoIIG promoter. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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25. Expression of spoIIIJ in the Prespone Is Sufficient for Activation of σ[sup G] and for Sporulation in Bacillus subtilis.
- Author
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Serrano, Mónica, Côrte, Luisa, Opdyke, Jason, Moran Jr., Charles P., and Henriques, Adriano O.
- Subjects
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BACILLUS subtilis , *BACTERIAL genetics , *GENE expression - Abstract
During sporulation in Bacillus subtilis, the prespore-specific developmental program is initiated soon after asymmetric division of the sporangium by the compartment-specific activation of RNA polymerase sigma factor σ[sup F] · σ[sup F] directs transcription of spoIIIG, encoding the late forespore-specific regulator σ[sup G]. Following synthesis, σ[sup G] is initially kept in an inactive form, presumably because it is bound to the SpoIIIAB anti-sigma factor. Activation of σ[sup G] occurs only after the complete engulfment of the prespore by the mother cell. Mutations in spoIIIJ arrest sporulation soon after conclusion of the engulfment process and prevent activation of σ[sup G]. Here we show that σ[sup G] accumulates but is mostly inactive in a spoIIIJ mutant. We also show that expression of the spoIIIGE155K allele, encoding a form of σ[sup G] that is not efficiently bound by SpoIIAB in vitro, restores σ[sup G]-directed gene expression to a spoIIIJ mutant. Expression of spoIIIJ occurs during vegetative growth. However, we show that expression of spoIIIJ in the prespore is sufficient for σ[sup G] activation and for sporulation. Mutations in the mother cell-specific spoIIIA locus are known to arrest sporulation just after completion of the engulfment process. Previous work has also shown that σ[sup G] accumulates in an inactive form in spoIIIA mutants and that the need for spoIIIA expression for σ[sup G] activation can be circumvented by the spoIIIGE155K allele. However, in contrast to the case for spoIIIJ, we show that expression of spoIIIA in the prespore does not support efficient sporulation. The results suggest that the activation of σ[sup G] at the end of the engulfment process involves the action of spoIIIA from the mother cell and of spoIIIJ from the prespore. [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
26. SpoVID Guides SafA to the Spore Coat in Bacillus subtilis.
- Author
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Ozin, Amanda J., Samford, Craig S., Henriques, Adriano O., and Moran Jr., Charles P.
- Subjects
- *
BACILLUS subtilis , *BACTERIAL spores - Abstract
Examines the assembly of the Bacillus subtilis spore coat and a spore-encasing proteinaceous structure. Formation of dormant cell type as an adaptive response to nutrient depletion; Interaction between SpoVID and another spore coat protein; Usage of immunofluorescence microscopy.
- Published
- 2001
- Full Text
- View/download PDF
27. Alternative Translation Initiation Produces a Short Form of a Spore Coat Protein in Bacillus....
- Author
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Ozin, Amanda J., Costa, Teresa, Henriques, Adriano O., and Moran Jr., Charles P.
- Subjects
- *
BACILLUS subtilis , *GENETIC translation , *PLASMID genetics - Abstract
Investigates endospore formation in Bacillus subtilis by alternative translation initiation. Expression of morphogenetic proteins; Presentation of SafA-c[sub 30] evidence from translation initiation at codon 164; Construction of plasmids containing mutations in the coding region.
- Published
- 2001
- Full Text
- View/download PDF
28. A LysM Domain Intervenes in Sequential Protein-Protein and Protein-Peptidoglycan Interactions Important for Spore Coat Assembly in Bacillus subtilis.
- Author
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Pereira, Fatima C., Nunes, Filipa, Cruz, Fernando, Fernandes, Catarina, Isidro, Anabela L., Lousa, Diana, Soares, Cláudio M., Moran Jr., Charles P., Henriques, Adriano O., and Serrano, Mónica
- Abstract
At a late stage in spore development in Bacillus subtilis, the mother cell directs synthesis of a layer of peptidoglycan known as the cortex between the two forespore membranes, as well as the assembly of a protective protein coat at the surface of the forespore outer membrane. SafA, the key determinant of inner coat assembly, is first recruited to the surface of the developing spore and then encases the spore under the control of the morphogenetic protein SpoVID. SafA has a LysM peptidoglycan-binding domain, SafALysM, and localizes to the cortex-coat interface in mature spores. SafALysM is followed by a region, A, required for an interaction with SpoVID and encasement. We now show that residues D10 and N30 in SafALysM, while involved in the interaction with peptidoglycan, are also required for the interaction with SpoVID and encasement. We further show that single alanine substitutions on residues S11, L12, and I39 of SafALysM that strongly impair binding to purified cortex peptidoglycan affect a later stage in the localization of SafA that is also dependent on the activity of SpoVE, a transglycosylase required for cortex formation. The assembly of SafA thus involves sequential protein-protein and protein-peptidoglycan interactions, mediated by the LysM domain, which are required first for encasement then for the final localization of the protein in mature spores. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
29. The Timing of cotE Expression Affects Bacillus subtilis Spore Coat Morphology but Not Lysozyme Resistance.
- Author
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Costa, Teresa, Serrano, Mónica, Steil, Leif, Völker, Uwe, Moran Jr., Charles P., and Henriques, Adriano O.
- Subjects
- *
BACILLUS subtilis , *BACTERIAL spores , *GENE expression , *LYSOZYMES , *GLYCOSIDASES - Abstract
The synthesis of structural components and morphogenetic factors required for the assembly of the Bacillus subtilis spore coat is governed by a mother cell-specific transcriptional cascade. The first two temporal classes of gene expression, which involve RNA polymerase sigma σE factor and the ancillary regulators GerR and SpoIIID, are deployed prior to engulfment of the prespore by the mother cell. The two last classes rely on σK, whose activation follows engulfment completion, and GerE. The cotE gene codes for a morphogenetic protein essential for the assembly of the outer coat layer and spore resistance to lysozyme. cotE is expressed first from a σE-dependent promoter and, in a second stage, from a promoter that additionally requires SpoIIID and that remains active under σK control. CotE localizes prior to engulfment completion close to the surface of the developing spore, but formation of the outer coat is a late, σK-controlled event. We have transplanted cotE to progressively later classes of mother cell gene expression. This created an early class of mutants in which cotE is expressed prior to engulfment completion and a late class in which expression of cotE follows the complete engulfment of the prespore. Mutants of the early class assemble a nearly normal outer coat structure, whereas mutants of the late class do not. Hence, the early expression of CotE is essential for outer coat assembly. Surprisingly, however, all mutants were fully resistant to lysozyme. The results suggest that CotE has genetically separable functions in spore resistance to lysozyme and spore outer coat assembly. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
30. Spo0A-Dependent Activation of an Extended -10 Region Promoter in Bacillus subtilis.
- Author
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Guangnan Chen, Kumar, Amrita, Wyman, Travis H., and Moran Jr., Charles P.
- Subjects
- *
BACILLUS subtilis , *DNA-binding proteins , *GENES , *CELLS , *GENETIC mutation - Abstract
At the onset of endospore formation in Bacillus subtilis the DNA-binding protein Spo0A directly activates transcription from promoters of about 40 genes. One of these promoters, Pskf, controls expression of an operon encoding a killing factor that acts on sibling cells. AbrB-mediated repression of Pskf provides one level of security ensuring that this promoter is not activated prematurely. However, Spo0A also appears to activate the promoter directly, since Spo0A is required for Pskf activity in a ΔabrB strain. Here we investigate the mechanism of Pskf activation. DNase I footprinting was used to determine the locations at which Spo0A bound to the promoter, and mutations in these sites were found to significantly reduce promoter activity. The sequence near the -10 region of the promoter was found to be similar to those of extended -10 region promoters, which contain a TRTGn motif. Mutational analysis showed that this extended -10 region, as well as other base pairs in the -10 region, is required for Spo0A-dependent activation of the promoter. We found that a substitution of the consensus base pair for the nonconsensus base pair at position -9 of Pskf produced a promoter that was active constitutively in both ΔabrB and Δspo0A ΔabrB strains. Therefore, the base pair at position -9 of Pskf makes its activity dependent on Spo0A binding, and the extended -10 region motif of the promoter contributes to its high level of activity. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
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