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Start Over Author "Paul T. Brey" Publisher american society for microbiology
4 results on '"Paul T. Brey"'

1. Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses

Author

Sarah Temmam, Thomas Bigot, Delphine Chrétien, Mathilde Gondard, Philippe Pérot, Virginie Pommelet, Evelyne Dufour, Stéphane Petres, Elodie Devillers, Thavry Hoem, Valérie Pinarello, Vibol Hul, Khamsing Vongphayloth, Jeffrey C. Hertz, Irène Loiseau, Marine Dumarest, Veasna Duong, Muriel Vayssier-Taussat, Marc Grandadam, Emmanuel Albina, Philippe Dussart, Sara Moutailler, Julien Cappelle, Paul T. Brey, and Marc Eloit

Subjects
Jingmenvirus, LIPS, emergence, evolution, Microbiology, QR1-502
Abstract

ABSTRACT Jingmenvirus is a recently identified group of segmented RNA viruses phylogenetically linked with unsegmented Flaviviridae viruses. Primarily identified in various tick genera originating in China, Jingmenvirus geographical distribution has rapidly expanded to cover Africa, South America, Caribbean, and Europe. The identification of Jingmen-related viruses in various mammals, including febrile humans, opens the possibility that Jingmenviruses may be novel tick-borne arboviruses. In this study, we aimed at increasing knowledge of the host range, genetic diversity, and geographical distribution of Jingmenviruses by reporting for the first time the identification of Jingmenviruses associated with Rhipicephalus microplus ticks originating in the French Antilles (Guadeloupe and Martinique islands), with Amblyomma testudinarium ticks in Lao PDR, and with Ixodes ricinus ticks in metropolitan France, and from urine of Pteropus lylei bats in Cambodia. Analyses of the relationships between the different Jingmenvirus genomes resulted in the identification of three main phylogenic subclades, each of them containing both tick-borne and mammal-borne strains, reinforcing the idea that Jingmenviruses may be considered as tick-borne arboviruses. Finally, we estimated the prevalence of Jingmenvirus-like infection using luciferase immunoprecipitation assay screening (LIPS) of asymptomatic humans and cattle highly exposed to tick bites. Among 70 French human, 153 Laotian human, and 200 Caribbean cattle sera tested, only one French human serum was found (slightly) positive, suggesting that the prevalence of Jingmenvirus human and cattle infections in these areas is probably low. IMPORTANCE Several arboviruses emerging as new pathogens for humans and domestic animals have recently raised public health concern and increased interest in the study of their host range and in detection of spillover events. Recently, a new group of segmented Flaviviridae-related viruses, the Jingmenviruses, has been identified worldwide in many invertebrate and vertebrate hosts, pointing out the issue of whether they belong to the arbovirus group. The study presented here combined whole-genome sequencing of three tick-borne Jingmenviruses and one bat-borne Jingmenvirus with comprehensive phylogenetic analyses and high-throughput serological screening of human and cattle populations exposed to these viruses to contribute to the knowledge of Jingmenvirus host range, geographical distribution, and mammalian exposure.

Published
2019
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2. Identification of a Novel Neuropathogenic Theiler's Murine Encephalomyelitis Virus

Author

Julian Buchrieser, Justine Cheval, Marc Eloit, Antoine Gessain, Simona Ozden, Meriadeg Ar-R. Gouilh, Laurence Fiette, Matthew R. Buckwalter, Paul T. Brey, Phan Thi Nga, Melissa E. Laird, Jean-Claude Manuguerra, Jean-François Bureau, Arnaud Fontanet, Matthew L. Albert, Immunobiologie des Cellules Dendritiques, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National d'Hygiène et d'Épidémiologie de Hanoi (NIHE), Réseau International des Instituts Pasteur (RIIP), Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats (CIBU), Institut Pasteur [Paris], Histopathologie humaine et Modèles animaux, Immunopathologie Virale, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Epidémiologie et Physiopathologie des Virus Oncogènes, Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Virologie UMR1161 (VIRO), Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-École nationale vétérinaire d'Alfort (ENVA), Institut Pasteur du Laos, Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM), Conservatoire National des Arts et Métiers [CNAM] (CNAM), This work was supported by grants from the Agence National de la Recherche (ANR) and an EURYI Award from the European Science Foundation (ESF)., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), Cellule d'Intervention Biologique d'Urgence (CIBU), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Epidémiologie des Maladies Emergentes, and Pasteur-Cnam risques infectieux et émergents (PACRI)

Subjects
MESH: Sequence Homology, Amino Acid, Neuropathogenic, viruses, MESH: Amino Acid Sequence, MESH: Spinal Cord, Mice, Demyelinating disease, MESH: Capsid, MESH: Animals, MESH: Phylogeny, Murine, Phylogeny, 0303 health sciences, biology, 030302 biochemistry & molecular biology, Brain, 3. Good health, Cardiovirus, Spinal Cord, Capsid, Theilovirus, Theiler’s, MESH: Genome, Viral, Molecular Sequence Data, Immunology, Genome, Viral, Microbiology, Virus, MESH: Brain, 03 medical and health sciences, MESH: Mice, Inbred C57BL, Virology, medicine, Encephalitis Viruses, Animals, Amino Acid Sequence, MESH: Mice, Gene, 030304 developmental biology, MESH: Molecular Sequence Data, Sequence Homology, Amino Acid, medicine.disease, biology.organism_classification, Mice, Inbred C57BL, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Viral Tropism, MESH: Theilovirus, Insect Science, Tissue tropism, Pathogenesis and Immunity, MESH: Viral Tropism
Abstract

Theiler's murine encephalitis viruses (TMEV) are divided into two subgroups based on their neurovirulence. Persistent strains resemble Theiler's original viruses (referred to as the TO subgroup), which largely induce a subclinical polioencephalomyelitis during the acute phase of the disease and can persist in the spinal cord of susceptible animals, inducing a chronic demyelinating disease. In contrast, members of the neurovirulent subgroup cause an acute encephalitis characterized by the rapid onset of paralysis and death within days following intracranial inoculation. We report herein the characterization of a novel neurovirulent strain of TMEV, identified using pyrosequencing technology and referred to as NIHE. Complete coverage of the NIHE viral genome was obtained, and it shares

Published
2011

3. Detection of Chikungunya Virus Antigen by a Novel Rapid Immunochromatographic Test

Author

Pongrama Ramasoota, Sarunyou Chusri, Tamaki Okabayashi, Ousmane Faye, Tadahiro Sasaki, Panjaporn Chaichana, Orapim Puiprom, Kazuyoshi Ikuta, Kris Cahyo Mulyantno, Paul T. Brey, Takeshi Kurosu, Seiji Kato, Mieko Kosaka, Abdourahmane Sow, Narong Nitatpattana, Sutee Yoksan, Amadou A. Sall, Nantarat Chantawat, Soegeng Soegijanto, Oumar Faye, Promsin Masrinoul, Ronald Enrique Morales Vargas, Marc Grandadam, Tomohiro Kotaki, and Siti Churrotin

Subjects
Serum, Microbiology (medical), Time Factors, viruses, Antibodies, Viral, medicine.disease_cause, Sensitivity and Specificity, Chromatography, Affinity, Virus, Dengue fever, Virus antigen, Antigen, Virology, Genotype, parasitic diseases, medicine, Animals, Humans, Chikungunya, Antigens, Viral, Mice, Inbred BALB C, Rapid diagnostic test, biology, Diagnostic Tests, Routine, business.industry, Antibodies, Monoclonal, virus diseases, Thailand, medicine.disease, Senegal, Author Corrections, Indonesia, Immunology, biology.protein, Chikungunya Fever, Antibody, business, Chikungunya virus
Abstract

Chikungunya fever is a mosquito-borne disease of key public health importance in tropical and subtropical countries. Although severe joint pain is the most distinguishing feature of chikungunya fever, diagnosis remains difficult because the symptoms of chikungunya fever are shared by many pathogens, including dengue fever. The present study aimed to develop a new immunochromatographic diagnosis test for the detection of chikungunya virus antigen in serum. Mice were immunized with isolates from patients with Thai chikungunya fever, East/Central/South African genotype, to produce mouse monoclonal antibodies against chikungunya virus. Using these monoclonal antibodies, a new diagnostic test was developed and evaluated for the detection of chikungunya virus. The newly developed diagnostic test reacted with not only the East/Central/South African genotype but also with the Asian and West African genotypes of chikungunya virus. Testing of sera from patients suspected to have chikungunya fever in Thailand (n= 50), Laos (n= 54), Indonesia (n= 2), and Senegal (n= 6) revealed sensitivity, specificity, and real-time PCR (RT-PCR) agreement values of 89.4%, 94.4%, and 91.1%, respectively. In our study using serial samples, a new diagnostic test showed high agreement with the RT-PCR within the first 5 days after onset. A rapid diagnostic test was developed using mouse monoclonal antibodies that react with chikungunya virus envelope proteins. The diagnostic accuracy of our test is clinically acceptable for chikungunya fever in the acute phase.

Published
2015

4. Correction for Okabayashi et al., Detection of Chikungunya Virus Antigen by a Novel Rapid Immunochromatographic Test

Author

Kazuyoshi Ikuta, Kris Cahyo Mulyantno, Panjaporn Chaichana, Orapim Puiprom, Siti Churrotin, Ronald Enrique Morales Vargas, Marc Grandadam, Promsin Masrinoul, Amadou A. Sall, Seiji Kato, Nantarat Chantawat, Soegeng Soegijanto, Ousmane Faye, Tomohiro Kotaki, Mieko Kosaka, Oumar Faye, Paul T. Brey, Narong Nitatpattana, Pongrama Ramasoota, Sarunyou Chusri, Takeshi Kurosu, Abdourahmane Sow, Tadahiro Sasaki, Sutee Yoksan, and Tamaki Okabayashi

Subjects
030203 arthritis & rheumatology, Microbiology (medical), 03 medical and health sciences, 0302 clinical medicine, Virus antigen, 030231 tropical medicine, Immunochromatographic test, medicine, Chikungunya, Biology, medicine.disease_cause, Virology
Abstract

Volume 53, no. 2, p. [382–388][1], 2015. Page 384, Fig. 1: Incorrect images were mistakenly placed in the second (Thai), fourth (S27), and sixth (SV) columns in the row labeled “Alphavirus Antibody.” The figure should appear as shown below. ![Figure][2] Page 385, Table 2: Several

Published
2016

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