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Your search keyword '"Wilson, Rebecca"' showing total 33 results
Start Over Author "Wilson, Rebecca" Publisher american society for microbiology
33 results on '"Wilson, Rebecca"'

1. Presence of a single genotype of the newly described species Mycobacterium immunogenum in industrial metalworking fluids associated with hypersensitivity pneumonitis

Author

Wallace, Richard J., Jr., Zhang, Yansheng, Wilson, Rebecca W., Mann, Linda, and Rossmoore, Harold

Subjects
Genotype -- Physiological aspects, Mycobacteria -- Physiological aspects, Mycobacterium, Fluids -- Environmental aspects, Rotating masses of fluid, Hypersensitivity pneumonitis -- Causes of, Biological sciences
Abstract

Research has been conducted on water-based metalworking fluids' mycobacteria causing outbreaks of hypersensitivity pneumonitis. The study of mycobacterial isolates from different plants with reported hypersensitivity pneumonitis cases has been carried out in an effort to determine whether those plants have been colonized with a single strain, and the results are described.

Published
2002

2. Hha is a negative modulator of transcription of hilA, the Salmonella enterica serovar Typhimurium invasion gene transcriptional activation

Author

Fahlen, Thomas F., Wilson, Rebecca L., Boddicker, Jennifer D., and Jones, Bradley D.

Subjects
Bacteriology -- Research, Genetic transcription -- Physiological aspects, Salmonella typhimurium -- Genetic aspects, Histidine -- Physiological aspects, Proteins -- Analysis, Phenotype -- Genetic aspects, Biological sciences
Abstract

Research has been conducted on the Salmonella invasive phenotype. The role of the histidine-like protein in the transcription and expression of this phenotype has been explored and the results are described.

Published
2001

3. DNA binding sites of the LysR-type regulator GcvA in the gcv and gcvA control regions of Escherichia coli

Author

Wilson, Rebecca L., Urbanowski, Mark L., and Stauffer, George V.

Subjects
Binding sites (Biochemistry) -- Analysis, Operons -- Analysis, Proteins -- Analysis, Biological sciences
Abstract

There are three binding sites in the glycine cleavage enzyme (gcv) operon of E. coli for GcvA: a 29-bp region from position 271 to 242, a 28-bp region from 242 to 214 and a 35-bp region from 69 to 34. GcvA is a protein that regulates the glycine cleavage enzyme (GCV) system by binding to the gcv operon. GCV cleaves glycine to form a methyl group that is used in the synthesis of purines and other cellular chemicals.

Published
1995

4. DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system

Author

Wilson, Rebecca L. and Stauffer, George V.

Subjects
DNA -- Research, Nucleotides -- Analysis, Genetic transcription -- Analysis, Biological sciences
Abstract

DNA sequence of the GcvA regulatory protein encoding gene contains an open reading frame with 930 nucleotides of molecular mass 34.4-kDa. Amino acid sequence of the GcvA protein is homologous to LysR activator proteins with the transcription site at 72-bp upstream of the translation site. Chromosomal deletion in GcvA inhibits gcvT-lacZ gene expression and fusion in the presence of inosine and glycine. Inosine and glycine do not influence the regulation of gcvA expression.

Published
1994

5. Roles of the GcvA and PurR proteins in negative regulation of the Escherichia coli glycine cleavage enzyme system

Author

Wilson, Rebecca L., Stauffer, Lorraine T., and Stauffer, George V.

Subjects
Escherichia coli -- Research, Glycine -- Evaluation, Biological sciences
Abstract

A reduction in the level of fully induced glycine cleavage enzyme showed the negative effects of GcvA and PurR on Escherichia Coli. The levels of beta galactosidase in cells with a lambda-GcvT-lacZ gene fusion were measured to judge the reduction levels of E. Coli. The repressor in Gcv regulation is directly involved in the process as seen by gel mobility shift and DNase I footprinting to bind purified PurR protein which affected a DNA fragment with the Gcv control region. The GcvA protein has two functions as it activates when glycine is present and represses when inosine is present. The GcvA gene positively activates the glycine cleavage enzyme system, causing extra repression.

Published
1993

6. Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat (MIRU-VNTR) Genotyping of Mycobacterium intracellulare for Strain Comparison with Establishment of a PCR-Based Database

Author

Iakhiaeva, Elena, McNulty, Steven, Brown-Elliott, Barbara A., Falkinham, Joseph O. III, Williams, Myra D., Vasireddy, Ravikiran, Wilson, Rebecca W., Turenne, Christine, Wallace, Richard J. Jr., Iakhiaeva, Elena, McNulty, Steven, Brown-Elliott, Barbara A., Falkinham, Joseph O. III, Williams, Myra D., Vasireddy, Ravikiran, Wilson, Rebecca W., Turenne, Christine, and Wallace, Richard J. Jr.

Abstract

Strain comparison is important to population genetics and to evaluate relapses in patients with Mycobacterium avium complex (MAC) lung disease, but the "gold standard" of pulsed-field gel electrophoresis (PFGE) is time-consuming and complex. We used variable-number tandem repeats (VNTR) for fingerprinting of respiratory isolates of M. intracellulare from patients with underlying bronchiectasis, to establish a nonsequence-based database for population analysis. Different genotypes identified by PFGE underwent species identification using a 16S rRNA gene multiplex PCR. Genotypes of M. intracellulare were confirmed by internal transcribed spacer 1 (ITS1) sequencing and characterized using seven VNTR primers. The pattern of VNTR amplicon sizes and repeat number defined each specific VNTR type. Forty-two VNTR types were identified among 84 genotypes. PFGE revealed most isolates with the same VNTR type to be clonal or exhibit similar grouping of bands. Repetitive sequence-based PCR (rep-PCR) showed minimal pattern diversity between VNTR types compared to PFGE. Fingerprinting of relapse isolates from 31 treated patients using VNTR combined with 16S multiplex PCR unambiguously and reliably distinguished different genotypes from the same patient, with results comparable to those of PFGE. VNTR for strain comparison is easier and faster than PFGE, is as accurate as PFGE, and does not require sequencing. Starting with a collection of 167 M. intracellulare isolates, VNTR distinguished M. intracellulare into 42 clonal groups. Comparison of isolates from different geographic areas, habitats, and clinical settings is now possible.

Published
2012
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7. Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat (MIRU-VNTR) Genotyping of Mycobacterium intracellulare for Strain Comparison with Establishment of a PCR-Based Database

Author

Biological Sciences, Iakhiaeva, Elena, McNulty, Steven, Brown-Elliott, Barbara A., Falkinham, Joseph O. III, Williams, Myra D., Vasireddy, Ravikiran, Wilson, Rebecca W., Turenne, Christine, Wallace, Richard J. Jr., Biological Sciences, Iakhiaeva, Elena, McNulty, Steven, Brown-Elliott, Barbara A., Falkinham, Joseph O. III, Williams, Myra D., Vasireddy, Ravikiran, Wilson, Rebecca W., Turenne, Christine, and Wallace, Richard J. Jr.

Abstract

Strain comparison is important to population genetics and to evaluate relapses in patients with Mycobacterium avium complex (MAC) lung disease, but the "gold standard" of pulsed-field gel electrophoresis (PFGE) is time-consuming and complex. We used variable-number tandem repeats (VNTR) for fingerprinting of respiratory isolates of M. intracellulare from patients with underlying bronchiectasis, to establish a nonsequence-based database for population analysis. Different genotypes identified by PFGE underwent species identification using a 16S rRNA gene multiplex PCR. Genotypes of M. intracellulare were confirmed by internal transcribed spacer 1 (ITS1) sequencing and characterized using seven VNTR primers. The pattern of VNTR amplicon sizes and repeat number defined each specific VNTR type. Forty-two VNTR types were identified among 84 genotypes. PFGE revealed most isolates with the same VNTR type to be clonal or exhibit similar grouping of bands. Repetitive sequence-based PCR (rep-PCR) showed minimal pattern diversity between VNTR types compared to PFGE. Fingerprinting of relapse isolates from 31 treated patients using VNTR combined with 16S multiplex PCR unambiguously and reliably distinguished different genotypes from the same patient, with results comparable to those of PFGE. VNTR for strain comparison is easier and faster than PFGE, is as accurate as PFGE, and does not require sequencing. Starting with a collection of 167 M. intracellulare isolates, VNTR distinguished M. intracellulare into 42 clonal groups. Comparison of isolates from different geographic areas, habitats, and clinical settings is now possible.

Published
2012

8. Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeat (MIRU-VNTR) Genotyping of Mycobacterium intracellulare for Strain Comparison with Establishment of a PCR-Based Database

Author

Iakhiaeva, Elena, primary, McNulty, Steven, additional, Brown Elliott, Barbara A., additional, Falkinham, Joseph O., additional, Williams, Myra D., additional, Vasireddy, Ravikiran, additional, Wilson, Rebecca W., additional, Turenne, Christine, additional, and Wallace, Richard J., additional

Published
2013
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10. Vaccination of BALB/c Mice withEscherichia coli-Expressed Vaccinia Virus Proteins A27L, B5R, and D8L Protects Mice from Lethal Vaccinia Virus Challenge

Author

Berhanu, Aklile, primary, Wilson, Rebecca L., additional, Kirkwood-Watts, Dana L., additional, King, David S., additional, Warren, Travis K., additional, Lund, Susan A., additional, Brown, Lindsay L., additional, Krupkin, Alex K., additional, VanderMay, Erin, additional, Weimers, Will, additional, Honeychurch, Kady M., additional, Grosenbach, Douglas W., additional, Jones, Kevin F., additional, and Hruby, Dennis E., additional

Published
2008
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11. Identification of a Novel Citrobacter rodentium Type III Secreted Protein, EspI, and Roles of This and Other Secreted Proteins in Infection

Author

Mundy, Rosanna, primary, Petrovska, Liljana, additional, Smollett, Katherine, additional, Simpson, Nandi, additional, Wilson, Rebecca K., additional, Yu, Jun, additional, Tu, Xuanlin, additional, Rosenshine, Ilan, additional, Clare, Simon, additional, Dougan, Gordon, additional, and Frankel, Gad, additional

Published
2007
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14. Polyphasic Characterization Reveals that the Human Pathogen Mycobacterium peregrinum Type II Belongs to the Bovine Pathogen Species Mycobacterium senegalense

Author

Wallace, Richard J., primary, Brown-Elliott, Barbara A., additional, Brown, June, additional, Steigerwalt, Arnold G., additional, Hall, Leslie, additional, Woods, Gail, additional, Cloud, Joann, additional, Mann, Linda, additional, Wilson, Rebecca, additional, Crist, Christopher, additional, Jost, Kenneth C., additional, Byrer, Dorothy E., additional, Tang, Jane, additional, Cooper, Jason, additional, Stamenova, Elena, additional, Campbell, Brian, additional, Wolfe, Joyce, additional, and Turenne, Christine, additional

Published
2005
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16. Clinical and Laboratory Features of Mycobacterium porcinum

Author

Wallace, Richard J., primary, Brown-Elliott, Barbara A., additional, Wilson, Rebecca W., additional, Mann, Linda, additional, Hall, Leslie, additional, Zhang, Yansheng, additional, Jost, Kenneth C., additional, Brown, June M., additional, Kabani, Amin, additional, Schinsky, Mark F., additional, Steigerwalt, Arnold G., additional, Crist, Christopher J., additional, Roberts, Glenn D., additional, Blacklock, Zeta, additional, Tsukamura, Michio, additional, Silcox, Vella, additional, and Turenne, Christine, additional

Published
2004
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17. Sequence-Based Identification of Aerobic Actinomycetes

Author

Patel, Jean Baldus, primary, Wallace, Richard J., additional, Brown-Elliott, Barbara A., additional, Taylor, Tony, additional, Imperatrice, Carol, additional, Leonard, Deborah G. B., additional, Wilson, Rebecca W., additional, Mann, Linda, additional, Jost, Kenneth C., additional, and Nachamkin, Irving, additional

Published
2004
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18. Identification of a Novel Citrobacter rodentium Type III Secreted Protein, EspI, and Roles of This and Other Secreted Proteins in Infection

Author

Mundy, Rosanna, primary, Petrovska, Liljana, additional, Smollett, Katherine, additional, Simpson, Nandi, additional, Wilson, Rebecca K., additional, Yu, Jun, additional, Tu, Xuanlin, additional, Rosenshine, Ilan, additional, Clare, Simon, additional, Dougan, Gordon, additional, and Frankel, Gad, additional

Published
2004
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24. Clinical and Laboratory Features of Mycobacterium mageritense

Author

Wallace, Richard J., primary, Brown-Elliott, Barbara A., additional, Hall, Leslie, additional, Roberts, Glenn, additional, Wilson, Rebecca W., additional, Mann, Linda B., additional, Crist, Christopher J., additional, Chiu, Sher H., additional, Dunlap, Robbie, additional, Garcia, Maria J., additional, Bagwell, J. Todd, additional, and Jost, Kenneth C., additional

Published
2002
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25. Identification of Major Outer Surface Proteins of Streptococcus agalactiae

Author

Hughes, Martin J. G., primary, Moore, Joanne C., additional, Lane, Jonathan D., additional, Wilson, Rebecca, additional, Pribul, Philippa K., additional, Younes, Zabin N., additional, Dobson, Richard J., additional, Everest, Paul, additional, Reason, Andrew J., additional, Redfern, Joanne M., additional, Greer, Fiona M., additional, Paxton, Thanai, additional, Panico, Maria, additional, Morris, Howard R., additional, Feldman, Robert G., additional, and Santangelo, Joseph D., additional

Published
2002
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32. Vaccination of BALB/c Mice with Escherichia coli-Expressed Vaccinia Virus Proteins A27L, B5R, and D8L Protects Mice from Lethal Vaccinia Virus Challenge.

Author

Berhanu, Aklile, Wilson, Rebecca L., Kirkwood-Watts, Dana L., King, David S., Warren, Travis K., Lund, Susan A., Brown, Lindsay L., Krupkin, Alex K., VanderMay, Erin, Weimers, Will, Honeychurch, Kady M., Grosenbach, Douglas W., Jones, Kevin F., and Hruby, Dennis E.

Subjects
*VACCINATION, *LABORATORY mice, *ESCHERICHIA coli, *PROTEINS, *VACCINIA
Abstract

The potential threat of smallpox use in a bioterrorist attack has heightened the need to develop an effective smallpox vaccine for immunization of the general public. Vaccination with the current smallpox vaccine, Dryvax, produces protective immunity but may result in adverse reactions for some vaccinees. A subunit vaccine composed of protective vaccinia virus proteins should avoid the complications arising from live-virus vaccination and thus provide a safer alternative smallpox vaccine. In this study, we assessed the protective efficacy and immunogenicity of a multisubunit vaccine composed of the A27L and D8L proteins from the intracellular mature virus (IMV) form and the B5R protein from the extracellular enveloped virus (EEV) form of vaccinia virus. BALB/c mice were immunized with Escherichia coli-produced A27L, D8L, and B5R proteins in an adjuvant consisting of monophosphoryl lipid A and trehalose dicorynomycolate or in TiterMax Gold adjuvant. Following immunization, mice were either sacrificed for analysis of immune responses or lethally challenged by intranasal inoculation with vaccinia virus strain Western Reserve. We observed that three immunizations either with A27L, D8L, and B5R or with the A27L and B5R proteins alone induced potent neutralizing antibody responses and provided complete protection against lethal vaccinia virus challenge. Several linear B-cell epitopes within the three proteins were recognized by sera from the immunized mice. In addition, protein-specific cellular responses were detected in spleens of immunized mice by a gamma interferon enzyme-linked immunospot assay using peptides derived from each protein. Our data suggest that a subunit vaccine incorporating bacterially expressed IMV- and EEV-specific proteins can be effective in stimulating anti-vaccinia virus immune responses and providing protection against lethal virus challenge. [ABSTRACT FROM AUTHOR]

Published
2008
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33. Listeria monocytogenes 10403S HtrA is necessary for resistance to cellular stress and virulence.

Author

Wilson RL, Brown LL, Kirkwood-Watts D, Warren TK, Lund SA, King DS, Jones KF, and Hruby DE

Subjects
Animals, Biofilms, Drug Resistance, Bacterial genetics, Hot Temperature, Listeria monocytogenes drug effects, Listeria monocytogenes enzymology, Listeria monocytogenes pathogenicity, Mice, Mice, Inbred BALB C, Puromycin pharmacology, Virulence genetics, Heat-Shock Proteins genetics, Listeria monocytogenes genetics, Oxidative Stress physiology, Periplasmic Proteins genetics, Serine Endopeptidases genetics
Abstract

The HtrA serine protease has been shown to be essential for bacterial virulence and for survival after exposure to many types of environmental and cellular stresses. A Listeria monocytogenes 10403S htrA mutant was found to be sensitive to oxidative and puromycin-induced stress at high temperatures, showed a reduced ability to form biofilms, and was attenuated for virulence in mice.

Published
2006
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