1. Establishment of Photoaging In Vitro by Repetitive UVA Irradiation: Induction of Characteristic Markers of Senescence and its Prevention by PAPLAL with Potent Catalase Activity.
- Author
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Yoshimoto S, Yoshida M, Ando H, and Ichihashi M
- Subjects
- Biomarkers metabolism, Cells, Cultured, Culture Media, Cyclin-Dependent Kinase Inhibitor p16 metabolism, Fibroblasts enzymology, Fibroblasts metabolism, Fibroblasts radiation effects, Humans, Hydrogen Peroxide metabolism, In Vitro Techniques, Skin cytology, Skin enzymology, Skin metabolism, beta-Galactosidase metabolism, Catalase metabolism, Metal Nanoparticles, Palladium chemistry, Platinum chemistry, Skin radiation effects, Skin Aging drug effects, Skin Aging radiation effects, Ultraviolet Rays
- Abstract
To understand a role of UVA radiation in photoaging of the skin, we established a model of photoaging cells using cultured human dermal fibroblasts. Repeated low-dose UVA radiation for 10 consecutive days induced senescence in fibroblasts, characterized with (1) increased level of senescence-associated β-galactosidase, (2) flattened large cell shape, (3) accumulation of reactive oxygen species, (4) yellowish coloration and (5) expression of p16. These were also observed in chronologically aged fibroblasts (doubling times >20), whereas none of these were detected in young cells (doubling times <10). Collectively, we propose that fibroblasts exposed to repetitive UVA radiation may be a good model of aged cells to study the mechanism of aging and photoaging and further to search for novel agents preventing cellular senescence. In addition, H
2 O2 was produced in the culture medium by a single low dose of UVA irradiation. Further, PAPLAL, a nanoparticle of platinum and palladium having potent catalase-like activity, significantly delayed the onset of H2 O2 -induced cell senescence. The present study strongly indicates that repetitive short-term UVA irradiation induces aging of cells possibly via H2 O2 and may be suppressed by potent anti-H2 O2 agents., (© 2017 The American Society of Photobiology.)- Published
- 2018
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