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Background and context: Human papilloma virus (HPV) has recently emerged as a new important etiological factor in the development of head and neck squamous cell carcinoma (HNSCC). The association of HPV in laryngeal squamous cell carcinoma (LSCC) is investigated in several studies but controversial results are established. Aim: This retrospective study aimed to evaluate the HPV DNA positivity in LSCC patients diagnosed and treated in 2 otorhinolaryngology referral center in northern region of Turkey. Strategy/Tactics: 52 formalin-fixed, paraffin-embedded (FFPE) tissue blocks of laryngeal cancers, diagnosed and treated between 2010 and 2016, were included. Detection and genotyping of HPV genotypes were done using a polymerase chain reaction (PCR) protocol. Program/Policy process: The study was planned as a retrospective investigation of laryngeal squamous cell cancer patients who had been diagnosed and treated in Samsun 19 Mayis University Hospital and Samsun Training and Research Hospital - otorhinolaryngology referral centers - between January 2010 and December 2016. Samsun is in the middle part of northern Turkey and stated as an oncology center in this region. Approval for the study was granted by the 19 Mayıs University Ethics Committee. The clinical characteristics of the patients were obtained from the computerized database. LSCC tissue samples fixed using 10% neutral buffered formalin and embedded blocks were used. Outcomes: PCR amplification was successful in 40 of 52 patients. Among the 40 LSCC samples HPV DNA was detected in 1 patient (2.5%). HPV 16 subtype was detected in this male patient aged 70 years, with a smoking history and stage III laryngeal cancer. After surgery, the patient received adjuvant radiotherapy and was still alive at 48 months without relapse. What was learned: In northern region of Turkey, this is the first study that evaluated HPV positivity in LSCC. Our results may suggest that HPV-related LSCC has not yet emerged as a significant health burden in our region. This finding may be due to the genetic, cultural or religious characteristics of our patients that are not conducive to oral HPV transmission. Unfortunately tobacco smoking is still the main reason for HNSCC in our city. There is a need for a nationwide screening study to investigate HPV prevalence variability among different regions in Turkey. [ABSTRACT FROM AUTHOR]