1. lncRNA HIF1A Antisense RNA 2 Modulates Trophoblast Cell Invasion and Proliferation through Upregulating PHLDA1 Expression
- Author
-
Yetao Xu, Dan Wu, Yuanyuan Zhang, Matthew Sagnelli, Bingqing Hui, Nana Yang, Lizhou Sun, Sailan Wang, and Zhenyao Huang
- Subjects
0301 basic medicine ,biology ,Cell growth ,lcsh:RM1-950 ,Cell migration ,Article ,Antisense RNA ,Cell biology ,03 medical and health sciences ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,0302 clinical medicine ,HIF1A ,Downregulation and upregulation ,Transcription (biology) ,030220 oncology & carcinogenesis ,Drug Discovery ,biology.protein ,Molecular Medicine ,Demethylase ,Chromatin immunoprecipitation - Abstract
Long noncoding RNAs (lncRNAs) have been reported to be involved in various human diseases, and increasing studies have revealed that lncRNAs can play a vital role in preeclampsia (PE). In our study, lncRNA hypoxia-inducible factor 1 alpha (HIF1A) antisense RNA 2 (HIF1A-AS2) was found to be significantly downregulated in placenta tissues of PE patients by quantitative real-time PCR analysis. Moreover, Cell Counting Kit-8 (CCK-8) assays showed that downregulation of HIF1A-AS2 can impede cell proliferation of HTR-8/SVneo and JAR trophoblasts cells. Ectopic overexpression of HIF1A-AS2 can increase the function of trophoblasts cell migration and invasion in vitro. RNA-sequencing (RNA-seq), RNA immunoprecipitation (RIP), and chromatin immunoprecipitation (ChIP) experiments showed that HIF1A-AS2 can recruit lysine-specific demethylase 1 (LSD1) and epigenetically repress pleckstrin homology-like domain, family A, member 1 (PHLDA1) transcription in human trophoblasts cells. In summary, our findings suggest that downregulated HIF1A-AS2 may play a role in the pathogenesis and progression of PE, and has potential as a novel prognostic marker in PE. Keywords: preeclampsia, HIF1A-AS2, LSD1, proliferation, invasion, PHLDA1
- Published
- 2019