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Start Over Author "Pal, Karol" Publisher american society of hematology
10 results on '"Pal, Karol"'

1. Somatic TP53 mutations are pre-leukemic events in acute lymphoblastic leukemia

Author

Chitadze, Guranda, primary, Stengel, Anna, additional, John-Klaua, Cathrin, additional, Bruckmüller, Julien, additional, Trautmann, Heiko, additional, Kotrova, Michaela, additional, Darzentas, Franziska, additional, Kelm, Miriam, additional, Pal, Karol, additional, Darzentas, Nikos, additional, Bastian, Lorenz, additional, Kehden, Britta, additional, Wessels, Wiebke, additional, Ströh, Aeint-Steffen, additional, Oberg, Hans-Heinrich, additional, Altrock, Philipp M, additional, Baer, Constance, additional, Meggendorfer, Manja, additional, Goekbuget, Nicola, additional, Baldus, Claudia D., additional, Haferlach, Claudia, additional, and Brüggemann, Monika, additional

Published
2022
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2. Genotyping and Minimal Residual Disease (MRD) Assessment in cfDNA By the Euroclonality-NGS DNA Capture (EC-NDC) Panel in Mantle Cell Lymphoma (MCL)

Author

Khouja, Mouhamad, primary, Genuardi, Elisa, additional, Ferrero, Simone, additional, Alessandria, Beatrice, additional, Verhagen, Onno, additional, Homburg, Christa, additional, van der Velden, Vincent H.J., additional, Gameiro, Paula, additional, García-Sanz, Ramón, additional, Medina, Alejandro, additional, Fronkova, Eva, additional, Brüggemann, Monika, additional, Dreyling, Martin, additional, Hoster, Eva, additional, Langerak, Anton, additional, Darzentas, Nikos, additional, Pal, Karol, additional, Stewart, Peter James, additional, Gonzalez, David, additional, and Pott, Christiane, additional

Published
2022
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3. Low-burden TP53 mutations in CLL: clinical impact and clonal evolution within the context of different treatment options

Author

Malcikova, Jitka, primary, Pavlova, Sarka, additional, Kunt Vonkova, Barbara, additional, Radova, Lenka, additional, Plevova, Karla, additional, Kotaskova, Jana, additional, Pal, Karol, additional, Dvorackova, Barbara, additional, Zenatova, Marcela, additional, Hynst, Jakub, additional, Ondrouskova, Eva, additional, Panovska, Anna, additional, Brychtova, Yvona, additional, Zavacka, Kristyna, additional, Tichy, Boris, additional, Tom, Nikola, additional, Mayer, Jiri, additional, Doubek, Michael, additional, and Pospisilova, Sarka, additional

Published
2021
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4. Validation of the EuroClonality-NGS DNA capture panel as an integrated genomic tool for lymphoproliferative disorders

Author

Stewart, James Peter, Gazdova, Jana, Darzentas, Nikos, Wren, Dorte, Proszek, Paula, Fazio, Grazia, Songia, Simona, Alcoceba, Miguel, Sarasquete, Maria Eugenia, Villarese, Patrick, Van Der Klift, Michele Y., Heezen, Kim C., McCafferty, Neil, Pal, Karol, Catherwood, Mark, Kim, Chang Sik, Srivastava, Shambhavi, Kroeze, Leonie I., Hodges, Elizabeth, Stamatopoulos, Kostas, Klapper, Wolfram, Genuardi, Elisa, Ferrero, Simone, Van Den Brand, Michiel, Cazzaniga, Giovanni, Davi, Frederic, Sutton, Lesley Ann, Garcia-Sanz, Ramon, Groenen, Patricia J.T.A., Macintyre, Elizabeth A., Bruggemann, Monika, Pott, Christiane, Langerak, Anton W., Gonzalez, David, Stewart, J, Gazdova, J, Darzentas, N, Wren, D, Proszek, P, Fazio, G, Songia, S, Alcoceba, M, Sarasquete, M, Villarese, P, Van Der Klift, M, Heezen, K, Mccafferty, N, Pal, K, Catherwood, M, Kim, C, Srivastava, S, Kroeze, L, Hodges, E, Stamatopoulos, K, Klapper, W, Genuardi, E, Ferrero, S, Van Den Brand, M, Cazzaniga, G, Davi, F, Sutton, L, Garcia-Sanz, R, Groenen, P, Macintyre, E, Bruggemann, M, Pott, C, Langerak, A, Gonzalez, D, and Immunology

Subjects
cohort analysi, Immunoglobulins, DNA determination, gene frequency, Article, high throughput sequencing, immunogenetic, single nucleotide polymorphism, B cell leukemia, genetic variability, carcinogenicity, Humans, diagnostic test accuracy study, human, lymphoproliferative disease, Gene Rearrangement, Lymphoid Neoplasia, limit of detection, bioinformatic, human cell, T cell leukemia, mutational analysi, copy number variation, DNA, Genomics, T-cell gene rearrangement, lymphocyte clone, major clinical study, Lymphoproliferative Disorders, Europe, clonal variation, health care quality, paraffin embedding, laboratory test, gene locu, diagnostic accuracy, chromosome translocation
Abstract

Current diagnostic standards for lymphoproliferative disorders include multiple tests for detection of clonal immunoglobulin (IG) and/or T-cell receptor (TCR) rearrangements, translocations, copy-number alterations (CNAs), and somatic mutations. The EuroClonality-NGS DNA Capture (EuroClonality-NDC) assay was designed as an integrated tool to characterize these alterations by capturing IGH switch regions along with variable, diversity, and joining genes of all IG and TCR loci in addition to clinically relevant genes for CNA and mutation analysis. Diagnostic performance against standard-of-care clinical testing was assessed in a cohort of 280 B- and T-cell malignancies from 10 European laboratories, including 88 formalin-fixed paraffin-embedded samples and 21 reactive lesions. DNA samples were subjected to the EuroClonality-NDC protocol in 7 EuroClonality-NGS laboratories and analyzed using a bespoke bioinformatic pipeline. The EuroClonality-NDC assay detected B-cell clonality in 191 (97%) of 197 B-cell malignancies and T-cell clonality in 71 (97%) of 73 T-cell malignancies. Limit of detection (LOD) for IG/TCR rearrangements was established at 5% using cell line blends. Chromosomal translocations were detected in 145 (95%) of 152 cases known to be positive. CNAs were validated for immunogenetic and oncogenetic regions, highlighting their novel role in confirming clonality in somatically hypermutated cases. Single-nucleotide variant LOD was determined as 4% allele frequency, and an orthogonal validation using 32 samples resulted in 98% concordance. The EuroClonality-NDC assay is a robust tool providing a single end-to-end workflow for simultaneous detection of B- and T-cell clonality, translocations, CNAs, and sequence variants.

Published
2021

5. Tumor and microenvironment response but no cytotoxic T-cell activation in classic Hodgkin lymphoma treated with anti-PD1

Author

Reinke, Sarah, primary, Bröckelmann, Paul J., additional, Iaccarino, Ingram, additional, Garcia-Marquez, Maria, additional, Borchmann, Sven, additional, Jochims, Franziska, additional, Kotrova, Michaela, additional, Pal, Karol, additional, Brüggemann, Monika, additional, Hartmann, Elena, additional, Sasse, Stephanie, additional, Kobe, Carsten, additional, Mathas, Stephan, additional, Soekler, Martin, additional, Keller, Ulrich, additional, Bormann, Matthias, additional, Zimmermann, Andreas, additional, Richter, Julia, additional, Fuchs, Michael, additional, von Tresckow, Bastian, additional, Borchmann, Peter, additional, Schlößer, Hans, additional, von Bergwelt-Baildon, Michael, additional, Rosenwald, Andreas, additional, Engert, Andreas, additional, and Klapper, Wolfram, additional

Published
2020
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6. Targeted Next Generation Sequencing Improves Diagnosis in Unclassifiable Leukemic Indolent B-Cell Non-Hodgkin Lymphoma and Identifies a Subset with Recurrent MYD88 Mutations in a Prospective Multicentre Study

Author

Cross, Matthew J, primary, Pietka, Grzegorz, additional, Dunne, Sarah, additional, Conneely, Leonora, additional, Hubank, Michael, additional, De Lord, Corinne, additional, Furtado, Michelle, additional, Kaczmarek, Pawel, additional, El-Sharkawi, Dima, additional, Dearden, Claire E., additional, Mohammed, Kabir, additional, Dunlop, Alan Stewart, additional, Morilla, Ricardo, additional, Pal, Karol, additional, Darzentas, Nikos, additional, Gonzalez, David, additional, Wren, Dorte, additional, and Iyengar, Sunil, additional

Published
2020
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7. Euroclonality-NGS DNA Capture Panel for Integrated Analysis of IG/TR Rearrangements, Translocations, Copy Number and Sequence Variation in Lymphoproliferative Disorders

Author

Stewart, Peter, primary, Gazdova, Jana, primary, Darzentas, Nikos, primary, Wren, Dorte, primary, Proszek, Paula, primary, Fazio, Grazia, primary, Songia, Simona, primary, Alcoceba, Miguel, primary, Sarasquete, Maria Eugenia, primary, Villarese, Patrick, primary, van der Klift, Michèle Y, primary, Heezen, Kim, primary, McCafferty, Neil, primary, Pal, Karol, primary, Catherwood, Mark, primary, Kim, Chang Sik, primary, Srivastava, Shambhavi, primary, Hodges, Elizabeth, primary, Stamatopoulos, Kostas, primary, Klapper, Wolfram, primary, Ferrero, Simone, primary, van den Brand, Michiel, primary, Cazzaniga, Giovanni, primary, Davi, Frederic, primary, Garcia-Sanz, Ramon, primary, Groenen, Patricia, primary, Macintyre, Elizabeth, primary, Brüggemann, Monika, primary, Pott, Christiane, primary, Genuardi, Elisa, primary, Langerak, Anton W, primary, and Gonzalez, David, primary

Published
2019
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8. Prognostic Impact of NOTCH1 Hotspot Mutation in TP53-Mutated Patients with Chronic Lymphocytic Leukemia

Author

Kantorova, Barbara, primary, Malcikova, Jitka, additional, Navrkalova, Veronika, additional, Smardova, Jana, additional, Brazdilova, Kamila, additional, Plevova, Karla, additional, Diviskova, Eva, additional, Borsky, Marek, additional, Pavlova, Sarka, additional, Tom, Nikola, additional, Pal, Karol, additional, Trbusek, Martin, additional, Oltova, Alexandra, additional, Brychtova, Yvona, additional, Doubek, Michael, additional, Mayer, Jiri, additional, and Pospisilova, Sarka, additional

Published
2014
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9. Mutational Analysis of TP53 Gene in Chronic Lymphocytic Leukemia: Comparison of Different Methodological Approaches

Author

Kantorova, Barbara, primary, Malcikova, Jitka, additional, Kminkova, Jana, additional, Navrkalova, Veronika, additional, Dvorakova, Barbora, additional, Tichy, Boris, additional, Smardova, Jana, additional, Trbusek, Martin, additional, Truong, Sim, additional, Stano-Kozubik, Katerina, additional, Tom, Nikola, additional, Pal, Karol, additional, Sebejova, Ludmila, additional, Patten, Nancy, additional, Wu, Lin, additional, Doubek, Michael, additional, Mayer, Jiri, additional, and Pospisilova, Sarka, additional

Published
2012
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10. Validation of the EuroClonality-NGS DNA capture panel as an integrated genomic tool for lymphoproliferative disorders.

Author

Stewart JP, Gazdova J, Darzentas N, Wren D, Proszek P, Fazio G, Songia S, Alcoceba M, Sarasquete ME, Villarese P, van der Klift MY, Heezen KC, McCafferty N, Pal K, Catherwood M, Kim CS, Srivastava S, Kroeze LI, Hodges E, Stamatopoulos K, Klapper W, Genuardi E, Ferrero S, van den Brand M, Cazzaniga G, Davi F, Sutton LA, Garcia-Sanz R, Groenen PJTA, Macintyre EA, Brüggemann M, Pott C, Langerak AW, and Gonzalez D

Subjects
DNA, Genomics, Humans, Immunoglobulins, Gene Rearrangement, Lymphoproliferative Disorders diagnosis, Lymphoproliferative Disorders genetics
Abstract

Current diagnostic standards for lymphoproliferative disorders include multiple tests for detection of clonal immunoglobulin (IG) and/or T-cell receptor (TCR) rearrangements, translocations, copy-number alterations (CNAs), and somatic mutations. The EuroClonality-NGS DNA Capture (EuroClonality-NDC) assay was designed as an integrated tool to characterize these alterations by capturing IGH switch regions along with variable, diversity, and joining genes of all IG and TCR loci in addition to clinically relevant genes for CNA and mutation analysis. Diagnostic performance against standard-of-care clinical testing was assessed in a cohort of 280 B- and T-cell malignancies from 10 European laboratories, including 88 formalin-fixed paraffin-embedded samples and 21 reactive lesions. DNA samples were subjected to the EuroClonality-NDC protocol in 7 EuroClonality-NGS laboratories and analyzed using a bespoke bioinformatic pipeline. The EuroClonality-NDC assay detected B-cell clonality in 191 (97%) of 197 B-cell malignancies and T-cell clonality in 71 (97%) of 73 T-cell malignancies. Limit of detection (LOD) for IG/TCR rearrangements was established at 5% using cell line blends. Chromosomal translocations were detected in 145 (95%) of 152 cases known to be positive. CNAs were validated for immunogenetic and oncogenetic regions, highlighting their novel role in confirming clonality in somatically hypermutated cases. Single-nucleotide variant LOD was determined as 4% allele frequency, and an orthogonal validation using 32 samples resulted in 98% concordance. The EuroClonality-NDC assay is a robust tool providing a single end-to-end workflow for simultaneous detection of B- and T-cell clonality, translocations, CNAs, and sequence variants., (© 2021 by The American Society of Hematology.)

Published
2021
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