1. Preliminary Molecular Characterization of Cryptosporidium parvum Isolates of Wildlife Rodents From Poland
- Author
-
Norman J. Pieniazek, J. M. Behnke, Małgorzata Bednarska, Anna Bajer, Simone M. Cacciò, and Edward Siński
- Subjects
Disease reservoir ,Genotype ,animal diseases ,Molecular Sequence Data ,Protozoan Proteins ,Cryptosporidiosis ,Animals, Wild ,Biology ,Polymerase Chain Reaction ,18S ribosomal RNA ,law.invention ,Microbiology ,Rodent Diseases ,law ,parasitic diseases ,RNA, Ribosomal, 18S ,Animals ,Ribosomal DNA ,Ecology, Evolution, Behavior and Systematics ,Polymerase chain reaction ,DNA Primers ,Disease Reservoirs ,Cryptosporidium parvum ,Genetics ,Base Sequence ,Arvicolinae ,Cryptosporidium ,Sequence Analysis, DNA ,DNA, Protozoan ,Ribosomal RNA ,biology.organism_classification ,Muridae ,Parasitology ,Poland - Abstract
Isolates of Cryptosporidium were collected from 3 species of woodland and field rodents (Clethrionomys glareolus, Microtus arvalis, and Apodemus flavicollis) and were characterized by polymerase chain reaction amplification and sequencing of fragments of the oocyst wall protein (COWP) gene and of the 18S ribosomal RNA gene. Sequence analysis of these markers revealed that the animals were infected with C. parvum, and that the genotype involved was almost identical to the mouse genotype previously described from Mus musculus. Thus, small rodents should be considered as an important reservoir of C. parvum genotypes closely related to the zoonotic genotype 2 and potentially hazardous to humans.
- Published
- 2003