1. Hormonemate Derivatives from Dothiora sp., an Endophytic Fungus.
- Author
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Pérez-Bonilla M, González-Menéndez V, Pérez-Victoria I, de Pedro N, Martín J, Molero-Mesa J, Casares-Porcel M, González-Tejero MR, Vicente F, Genilloud O, Tormo JR, and Reyes F
- Subjects
- Antineoplastic Agents chemistry, Drug Screening Assays, Antitumor, Esters pharmacology, Hep G2 Cells, Humans, Molecular Structure, Nuclear Magnetic Resonance, Biomolecular, Antineoplastic Agents isolation & purification, Antineoplastic Agents pharmacology, Ascomycota chemistry, Asteraceae chemistry
- Abstract
A search for cytotoxic agents from cultures of the endophytic fungus Dothiora sp., isolated from the endemic plant Launaea arborescens, led to the isolation of six new compounds structurally related to hormonemate, with moderate cytotoxic activity against different cancer cell lines. By using a bioassay-guided fractionation approach, hormonemates A-D (1-4), hormonemate (5), and hormonemates E (6) and F (7) were obtained from the acetone extract of this fungus. Their structures were determined using a combination of HRMS, ESI-qTOF-MS/MS, 1D and 2D NMR experiments, and chemical degradation. The cytotoxic activities of these compounds were evaluated by microdilution colorimetric assays against human breast adenocarcinoma (MCF-7), human liver cancer cells (HepG2), and pancreatic cancer cells (MiaPaca_2). Most of the compounds displayed cytotoxic activity against this panel.
- Published
- 2017
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