1. Dengue-3 outbreak in Paraguay: investigations using capillary blood samples on filter paper.
- Author
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Matheus S, Meynard JB, Lavergne A, Girod R, Moua D, Labeau B, Dussart P, Lacoste V, and Deparis X
- Subjects
- Antigens, Viral blood, Dengue blood, Dengue Virus classification, Dengue Virus genetics, Dengue Virus immunology, Enzyme-Linked Immunosorbent Assay, Humans, Paper, Paraguay epidemiology, Reverse Transcriptase Polymerase Chain Reaction, Dengue epidemiology, Dengue Virus isolation & purification, Disease Outbreaks
- Abstract
During a dengue-3 outbreak in Paraguay at the beginning of 2007, capillary blood samples absorbed onto filter papers were collected from 44 suspected cases. These samples were subjected to three molecular and serologic tests, and 31 of the 44 samples gave a positive result by at least one of the techniques used. Molecular analyses detected the dengue-3 serotype in 22 patients and additionally the dengue-2 serotype in two patients. Therefore two different serotypes were co-circulating during this outbreak. Overall, this study validates the use of dried-blood samples for field screening investigations. Indeed, all types of laboratory studies of dengue were possible with samples consisting of a few drops of dried blood from finger pricks.
- Published
- 2008