1. Molecular epidemiology of dengue type 3 virus in Brazil and Paraguay, 2002-2004.
- Author
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Aquino VH, Anatriello E, Gonçalves PF, DA Silva EV, Vasconcelos PF, Vieira DS, Batista WC, Bobadilla ML, Vazquez C, Moran M, and Figueiredo LT
- Subjects
- 3' Untranslated Regions chemistry, Amino Acid Sequence, Base Sequence, Brazil epidemiology, Consensus Sequence, DNA, Complementary chemistry, DNA, Viral chemistry, Dengue virology, Dengue Virus classification, Humans, Paraguay epidemiology, Phylogeny, RNA, Viral genetics, RNA, Viral isolation & purification, Reverse Transcriptase Polymerase Chain Reaction, Viral Envelope Proteins chemistry, Dengue epidemiology, Dengue Virus genetics, Viral Envelope Proteins genetics
- Abstract
We studied the molecular epidemiology of dengue virus type 3 (DENV-3) in Brazil and Paraguay by analyzing the 5' and 3' untranslated regions (5' and 3'UTRs) and the E protein gene of viruses isolated between 2002 and 2004. Both 5' and 3'UTRs were highly conserved. However, the 3'UTR of two isolates from Brazil contained eight nucleotide deletions compared with the remaining 26 viruses. Phylogenetic analyses suggested that DENV-3 was introduced into Brazil from the Caribbean Islands at least twice and into Paraguay from Brazil at least three times.
- Published
- 2006