1. Reference values for Leishmania infantum parasitemia in different clinical presentations: quantitative polymerase chain reaction for therapeutic monitoring and patient follow-up.
- Author
-
Mary C, Faraut F, Drogoul MP, Xeridat B, Schleinitz N, Cuisenier B, and Dumon H
- Subjects
- Amphotericin B therapeutic use, Animals, DNA, Protozoan analysis, DNA, Protozoan blood, Follow-Up Studies, Leishmania infantum genetics, Leishmaniasis, Visceral drug therapy, Leishmaniasis, Visceral parasitology, Parasitemia blood, Parasitemia parasitology, Polymerase Chain Reaction methods, Recurrence, Reference Values, Sensitivity and Specificity, Leishmania infantum isolation & purification, Leishmaniasis, Visceral diagnosis, Parasitemia diagnosis, Parasitemia epidemiology, Polymerase Chain Reaction veterinary
- Abstract
Quantification of Leishmania infantum DNA in blood samples by an ultrasensitive quantitative polymerase chain reaction (QPCR) detected parasitemias in different clinical presentations. We observed a large range of parasitemias, more than 9 log values, and could determine the threshold between asymptomatic carriage and disease in the Mediterranean area (approximately one parasite/mL of blood). Based on kinetoplast DNA amplification, this assay had a sensitivity of 0.001 parasite DNA equivalents/mL and detected asymptomatic carriage of Leishmania. It detected parasite DNA in 58% of healthy subjects, while an immunoblot detected specific antibodies in only 16%. For initial diagnosis of disease, this quantitative PCR with blood samples constitutes a non-invasive alternative to bone marrow aspiration. Its main applications are monitoring of drug therapy and follow-up of immunodeficient patients for biologic confirmation of relapses.
- Published
- 2006