1. Replica moulded poly(dimethylsiloxane) microwell arrays induce localized endothelial cell immobilization for coculture with pancreatic islets
- Author
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Anouck L.S. Burzava, Aurelien Forget, Claudine S. Bonder, Thomas W.H. Kay, Paula Facal Marina, Darling Rojas-Canales, Frances J. Harding, Nicolas H. Voelcker, P. Toby Coates, Helen E. Thomas, Thomas Loudovaris, Vincent Ahmadi, Camille Rouzaud, Anton Blencowe, Michaelia P. Cockshell, Daniella Penko, Michaela Waibel, Forget, Aurelien, Harding, Frances J, Cockshell, Michaelia P, Penko, Daniella, Rouzaud, Camille, Ahmadi, Vincent, Marina, Paula F, Rojas-Canales, Darling, Bonder, Claudine S, Coates, P Toby H, Waibel, Michaela, Thomas, Helen E, Kay, Thomas W, Loudovaris, Thomas, Blencowe, Anton, and Voelcker, Nicolas H
- Subjects
Materials science ,Surface Properties ,education ,Population ,cell adhesive properties ,General Physics and Astronomy ,General Biochemistry, Genetics and Molecular Biology ,Biomaterials ,Islets of Langerhans ,PolyJet three-dimensional (3D) printing ,Insulin-Secreting Cells ,Cell Adhesion ,medicine ,Surface roughness ,Humans ,General Materials Science ,Dimethylpolysiloxanes ,Cell adhesion ,chemistry.chemical_classification ,education.field_of_study ,Pancreatic islets ,Replica ,Endothelial Cells ,General Chemistry ,Polymer ,Cells, Immobilized ,3D moulds ,Coculture Techniques ,Endothelial stem cell ,medicine.anatomical_structure ,chemistry ,Glucagon-Secreting Cells ,Printing, Three-Dimensional ,Adhesive ,Biomedical engineering - Abstract
PolyJet three-dimensional (3D) printing allows for the rapid manufacturing of 3D moulds for the fabrication of cross-linked poly(dimethylsiloxane) microwell arrays (PMAs). As this 3D printing technique has a resolution on the micrometer scale, the moulds exhibit a distinct surface roughness. In this study, the authors demonstrate by optical profilometry that the topography of the 3D printed moulds can be transferred to the PMAs and that this roughness induced cell adhesive properties to the material. In particular, the topography facilitated immobilization of endothelial cells on the internal walls of the microwells. The authors also demonstrate that upon immobilization of endothelial cells to the microwells, a second population of cells, namely, pancreatic islets could be introduced, thus producing a 3D coculture platform Refereed/Peer-reviewed
- Published
- 2019
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