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Leaf rust caused by Pucinia recondita tritici is one of the most important diseases of bread wheat worldwide. It is considered that the most environmentally sound; low cost method of controlling leaf rust is to breed and grow genetically resistant wheat varieties. In the research, twenty-four bread wheat varieties grown intensively were used as genetic material in Trakya Region where the North-West Part of Turkey. To create artificial leaf rust epidemic in field conditions, two sensitive varieties (Morrocco and Cumhuriyet 75) was sown after each ten genotypes, and the reactions of the varieties to leaf rust were investigated in field conditions. Isogenic lines carrying the genes Lr9, Lr14, Lr19, Lr24 and Lr47 from CIMMYT were used as control genotypes in molecular analysis. In the field conditions, although Pehlivan, Selimiye, Sagittario, Tina, Anapo, Montchill and Saraybosna were the most sensitive genotypes, Nota, Kate A1, Prostor and Sana were the most resistant bread wheat varieties to leaf rust. It was determined that Sana, Pehlivan, Golia, Falmura 85, Saroz 95, Renan, Sirena, Kate A1, Selimiye, Bezostoja 1, Saraybosna, Nina and Tina varieties have Lr9 gene with SSR analysis. It has been observed that all bread wheat varieties carry Lr14, Lr19, Lr24 and Lr47 (except Krasunia, Aldane and Gelibolu varieties) genes. It is revealed that Lr9 and Lr47 genes should be taken into consideration in the studies to be performed in the region and these genes will be useful to examine together with a larger number of leaf rust genes for more successful results in breeding studies. © Ankara Üniversitesi Ziraat Fakültesi.

Namık Kemal Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümünde yürütülen bu araştırmada, iki makarnalık buğday çeşidine uygulanan 6 farklı gamma ışını dozunun M1 ve M2 generasyonlarında bitki gelişimi üzerine etkisi araştırılmıştır. M2 generasyonunda incelenen 7 karakter yönünden mutant ve kontrol genotipler karşılaştırıldığında, birçok özellik bakımından istenen mutant tipler elde edilmiştir. Özellikle 200 gray doz uygulamasından sonra seçilen mutant genotipler arasında bitki boyu yönünden önemli oranda kısalma gösteren genotipler elde edilmiştir. Bitki verimi, ana saptaki başakta tane ağırlığı, başakta tane sayısı, hasat indeksi, başak uzunluğu yönünden 300 gray doz uygulamalarında istenen özellikleri taşıyan genotipler ortaya çıkmıştır. Ayrıca, mutagen uygulamasının bitkide kardeş sayısını artırdığı belirlenmiştir. Kontrol ve seçilen mutant genotiplerde SDS-PAGE yöntemiyle elde edilen protein bandları yönünden özellikle 300-500 gray dozlarında farklılıklar gözlenmiştir, In this research conducted in the Department of Field Crops, Agricultural Faculty, Namık Kemal University, the effect of six different gamma ray doses on plant growth in M1 and M2 generations derived from two durum wheat cultivars was investigated. When mutants and control genotypes in M2 generation were investigated for seven characters, a considerable number of mutants having the desired characteristics were obtained. The application of 200 gray dose resulted in significantly short genotypes among selected mutant genotypes. After 300 gray dose applications, suitable genotypes were obtained in terms of plant height, seed yield per main spike, the number of seeds per spike, harvesting index and spike length. In addition, mutagen application increased number of tillers per plant. For obtained protein band design using the SDS-PAGE method in the Standard and mutant durum wheat genotypes were observed different in 300, 400 and 500 gray mutagen doses