1. Fungal Protein Production: Design and Production of Chimeric Proteins
- Author
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Jan Wery, Hans Visser, Eric Record, Peter J. Punt, Anthony Levasseur, TNO, Unité mixte de recherche de biotechnologie des champignons filamenteux, Université de Provence - Aix-Marseille 1-Institut National de la Recherche Agronomique (INRA)-Université de la Méditerranée - Aix-Marseille 2, DYADIC Nederland, and Partenaires INRAE
- Subjects
DOCKERIN DOMAIN ,CAZy ,purification ,[SDV]Life Sciences [q-bio] ,CLOSTRIDIUM-STERCORARIUM ,FERULOYL-ESTERASE-A ,Heterologous ,ASPERGILLUS-NIGER ,Computational biology ,Biology ,Microbiology ,Chrysosporium ,Fungal Proteins ,03 medical and health sciences ,PIROMYCES-EQUI ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,CAZY ,fusion protein ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,CHRYSOSPORIUM-LUCKNOWENSE ,carbohydrate-binding module ,030304 developmental biology ,Trichoderma ,chemistry.chemical_classification ,0303 health sciences ,Fungal protein ,030306 microbiology ,Fungi ,CARBOHYDRATE-BINDING MODULES ,Special class ,Fusion protein ,Recombinant Proteins ,Aspergillus ,Enzyme ,chemistry ,PYCNOPORUS-CINNABARINUS ,Endogenous enzymes ,Carbohydrate-binding module ,Genetic Engineering ,PLANT-CELL WALLS ,DOCKING DOMAINS ,Biotechnology - Abstract
International audience; For more than a century, filamentous fungi have been used for the production of a wide variety of endogenous enzymes of industrial interest. More recently, with the use of genetic engineering tools developed for these organisms, this use has expanded for the production of nonnative heterologous proteins. In this review, an overview is given of examples describing the production of a special class of these proteins, namely chimeric proteins. The production of two types of chimeric proteins have been explored: (a) proteins grafted for a specific substrate-binding domain and (b) fusion proteins containing two separate enzymatic activities. Various application areas for the use of these chimeric proteins are described.
- Published
- 2011