1. Expression Levels of PIWI-interacting RNA, piR-823, Are Deregulated in Tumor Tissue, Blood Serum and Urine of Patients with Renal Cell Carcinoma
- Author
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Robert Iliev, Dalibor Pacík, Ondrej Slaby, Michal Staník, Hana Mlčochová, Tana Machackova, Marek Svoboda, Jan Dolezel, and Michal Fedorko
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Adult ,0301 basic medicine ,Cancer Research ,Pathology ,medicine.medical_specialty ,medicine.medical_treatment ,Urinary system ,Biology ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Blood serum ,Circulating tumor cell ,Renal cell carcinoma ,Biomarkers, Tumor ,medicine ,Humans ,RNA, Small Interfering ,Carcinoma, Renal Cell ,Aged ,Aged, 80 and over ,Kidney ,Area under the curve ,General Medicine ,Middle Aged ,medicine.disease ,Kidney Neoplasms ,Nephrectomy ,3. Good health ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Area Under Curve ,030220 oncology & carcinogenesis ,Kidney cancer - Abstract
Background: Renal cell carcinoma (RCC) is the most common neoplasm of adult kidney accounting for about 3% of adult malignancies. P-Element induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are a new class of naturally occurring, short non-coding RNAs involved in silencing of transposable elements and in sequence-specific chromatin modifications. There were preliminary data published indicating that piR-823 expression is deregulated in circulating tumor cells and tumor tissue in gastric and kidney cancer, respectively. Patients and Methods: In our study, we analyzed piR-823 levels in 588 biological specimens: tumor tissue (N=153), adjacent renal parenchyma (N=121), blood serum (N=178) and urine (N=20) of patients undergoing nephrectomy for RCC; and in blood serum (N=101) and urine (N=15) of matched healthy controls. Expression levels of piR-823 were determined in all biological specimens by quantitative real-time polymerase chain reaction, compared in patients and controls, and correlated with clinicopathological features of RCC. Results: We identified a significant downregulation of piR-823 in tumor tissue [p
- Published
- 2016
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