1. CUB Domain-containing Protein 1 (CDCP1) Is Down-regulated by Active Hexose-correlated Compound in Human Pancreatic Cancer Cells
- Author
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Junichi Hamada, Masayuki Tokunaga, Tohru Ohta, Masaru Terasaki, Yasuhiro Kuramitsu, Takao Kitagawa, Tsuyoshi Shimo, Byron Baron, Masanobu Kobayashi, Osamu Uehara, Hiroki Nagayasu, Koji Harada, Keisuke Kuhara, Rie Takai, and Kazuhiro Tokuda
- Subjects
0301 basic medicine ,Cancer Research ,Blotting, Western ,Down-Regulation ,Deoxycytidine ,03 medical and health sciences ,0302 clinical medicine ,Hsp27 ,Western blot ,Antigens, CD ,Antigens, Neoplasm ,Polysaccharides ,Cell Line, Tumor ,Pancreatic cancer ,Active hexose correlated compound ,medicine ,Humans ,HSF1 ,biology ,medicine.diagnostic_test ,Chemistry ,General Medicine ,CUB domain ,medicine.disease ,Gemcitabine ,Molecular biology ,Actins ,Neoplasm Proteins ,Pancreatic Neoplasms ,030104 developmental biology ,Oncology ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,biology.protein ,CDCP1 ,Antibody ,Cell Adhesion Molecules - Abstract
BACKGROUND/AIM We have previously reported that treatment of pancreatic cancer cells with active hexose-correlated compound (AHCC), an extract of a basidiomycete mushroom, decreases the levels of tumor-associated proteins including heat-shock protein 27 (HSP27), heat shock factor 1 (HSF1) and sex-determining region Y-box 2 (SOX2). The transmembrane glycoprotein, CUB domain-containing protein 1 (CDCP1) has been reported to be up-regulated in various cancers, and be associated with invasion and metastasis. The aim of this study was to examine the effect of AHCC on the expression of CDCP1 in KLM1-R cells. MATERIALS AND METHODS Gemcitabine-resistant pancreatic cancer cells (KLM1-R) were treated with AHCC (10 mg/ml) for 48 h. Western blot analysis of cell extracts with anti-CDCP1 or anti-actin antibodies was performed to assess the expression of CDCP1. RESULTS Expression of CDCP1 was reduced by AHCC treatment of KLM1-R cells, whereas expression of actin was not affected. The ratio of intensities of CDCP1/actin in AHCC-treated KLM1-R cells was significantly suppressed (p
- Published
- 2018
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