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An accurate, simple and reproducible liquid chromatographic method was developed and validated for the determination of tacrolimus in capsules. The analysis were performed at room temperature on a reverse phase C18 column with UV detection at 210 nm. The mobile phase consisted of methanol-water (90 + 10) at a constant flow rate of 0.8 mL/min. The method was validated in terms of linearity, precision, accuracy and specificity by forced decomposition of tacrolimus using acid, base, water, hydrogen peroxide, heat and light. The response was linear in the range of 0.09-0.24 mg/mL (r2 = 0.9997). The relative standard deviation values for intra- and interday precision studies were 1.28 and 2.91. Recoveries ranged between 98.06 and 102.52 %. Fil: Moyano, Maria Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Simionato, Laura Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Pizzorno, Maria Teresa. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina Fil: Segall, Adriana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina