1. Effect of Dipeptides on In vitro Maturation, Fertilization and Subsequent Embryonic Development of Porcine Oocytes
- Author
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Inho Choi, Quzi Sharmin Akter, M.A.M. Yahia Khandoker, Hirotada Tsujii, and K. M. A. Tareq
- Subjects
Embryogenesis ,Embryo ,Biology ,Oocyte ,Follicular fluid ,In vitro maturation ,Andrology ,Tissue culture ,Human fertilization ,medicine.anatomical_structure ,Biochemistry ,medicine ,Animal Science and Zoology ,Blastocyst ,Food Science - Abstract
The effects of amino acids and dipeptides on in vitro production of porcine embryos and accumulation of ammonia in culture medium during developmental stages were examined in this study. The maturation, fertilization and development of embryonic cultures were performed in modified Tissue culture medium (mTCM)-199 supplemented with 10% (v/v) porcine follicular fluid, modified Tyrode's albumin lactate pyruvate (mTALP) medium, and modified North Carolina State University (mNCSU)-23 medium, respectively. In addition, amino acids and dipeptides of different concentrations and combinations were used to treat the embryos. The addition of L-alanyl-L-glutamine (AlnGln)L-glycyl-L-glutamine (GlyGln) significantly (p
- Published
- 2013
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