1. Novel Nonsense Variants c.58C>T (p.Q20X) and c.256G>T (p.E85X) in the CHEK2 Gene Identified dentified in Breast Cancer Patients from Balochistan
- Author
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Ahmad Nawaz Khosa, Dost Mohammad Baloch, Hafiz Khush Naseeb, Illahi Bakhsh Marghazani, Nasrullah Bangulzai, Abdul Hameed Baloch, Abdul Majeed Cheema, Jamila Shuja, Mohammad Jan, Jamil Ahmad, and Masood-ul Haq Kakar
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Adult ,0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Epidemiology ,DNA damage ,media_common.quotation_subject ,Nonsense ,Breast Neoplasms ,medicine.disease_cause ,03 medical and health sciences ,Exon ,0302 clinical medicine ,Breast cancer ,Internal medicine ,medicine ,Humans ,Genetic Predisposition to Disease ,Pakistan ,skin and connective tissue diseases ,CHEK2 ,Neoplasm Staging ,media_common ,Gynecology ,Mutation ,business.industry ,Public Health, Environmental and Occupational Health ,Cancer ,Exons ,Middle Aged ,medicine.disease ,Penetrance ,Checkpoint Kinase 2 ,030104 developmental biology ,Codon, Nonsense ,030220 oncology & carcinogenesis ,Female ,business - Abstract
Breast cancer is the most commonly occurring and leading cause of cancer deaths among women globally. Hereditary cases account 5-10% of all the cases and CHEK2 is considered as a moderate penetrance breast cancer risk gene. CHEK2 plays a crucial role in response to DNA damage to promote cell cycle arrest and repair DNA damage or induce apoptosis. Our objective in the current study was to analyze mutations in the CHEK2 gene related to breast cancer in Balochistan. A total of 271 individuals including breast cancer patients and normal subjects were enrolled. All 14 exons of CHEK2 were amplified and sequenced. The majority of the patients (>95%) had invasive ductal carcinomas (IDCs), 52.1% were diagnosed with tumor grade III and 56.1% and 27.5% were diagnosed with advance stages III and IV. Two novel nonsense variants i.e. c.58C>T (P.Q20X) and c.256G>T (p.E85X) at exon 1 and 2 in two breast cancer patients were identified in the current study. Both the variants identified were novel and have not been reported elsewhere.
- Published
- 2016
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