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Start Over Author "Moons, Lieve" Publisher association for research in vision and ophthalmology (arvo)
12 results on '"Moons, Lieve"'

4. Aberrant Collagen Composition of the Trabecular Meshwork Results in Reduced Aqueous Humor Drainage and Elevated IOP in MMP-9 Null Mice

Author

De Groef, Lies, primary, Andries, Lien, additional, Siwakoti, Anuja, additional, Geeraerts, Emiel, additional, Bollaerts, Ilse, additional, Noterdaeme, Lut, additional, Etienne, Isabelle, additional, Papageorgiou, Anna-Pia, additional, Stalmans, Ingeborg, additional, Billen, Johan, additional, West-Mays, Judith A., additional, and Moons, Lieve, additional

Published
2016
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8. AMA0076, a Novel, Locally Acting Rho Kinase Inhibitor, Potently Lowers Intraocular Pressure in New Zealand White Rabbits with Minimal Hyperemia

Author

Van de Velde, Sarah, primary, Van Bergen, Tine, additional, Sijnave, Davine, additional, Hollanders, Karolien, additional, Castermans, Karolien, additional, Defert, Olivier, additional, Leysen, Dirk, additional, Vandewalle, Evelien, additional, Moons, Lieve, additional, and Stalmans, Ingeborg, additional

Published
2014
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12. The effect of AMA0428, a novel and potent ROCK inhibitor, in a model of neovascular age-related macular degeneration.

Author

Hollanders K, Van Bergen T, Kindt N, Castermans K, Leysen D, Vandewalle E, Moons L, and Stalmans I

Subjects
Animals, Apoptosis, Cell Line, Cell Movement, Cell Proliferation, Disease Models, Animal, Humans, Macular Degeneration etiology, Macular Degeneration pathology, Male, Mice, Mice, Inbred C57BL, Retinal Neovascularization etiology, Retinal Neovascularization pathology, Vascular Endothelial Growth Factor A antagonists & inhibitors, Vascular Endothelial Growth Factor A metabolism, Enzyme Inhibitors therapeutic use, Macular Degeneration drug therapy, Retinal Neovascularization complications, rho-Associated Kinases antagonists & inhibitors
Abstract

Purpose: Rho kinase (ROCK) is associated with VEGF-driven angiogenesis, as well as with inflammation and fibrosis. Therefore, the effect of AMA0428, a novel ROCK inhibitor, was studied in these processes, which highly contribute to the pathogenesis of neovascular AMD., Methods: The effect of AMA0428 (0.5-5.0 μM) on human umbilical vein endothelial cells (HUVECs), human brain microvascular endothelial cells (HBMECs), and human brain microvascular pericytes (HBVPs) was determined using cell viability (WST-1), apoptosis (caspase 3/7), and migration (scratch and under-agarose) assays. The in vivo response was investigated using a laser-induced choroidal neovascularization (CNV) mouse model, in which intravitreal injections of AMA0428, murine anti-VEGF-R2 mAb (DC101), or placebo was given. Outcome was assessed by analysis of inflammation (CD45), angiogenesis (FITC-dextran), vessel leakage (Texas Red-conjugated Dextran and FITC-labeled lectin) and fibrosis (Sirius Red/Collagen I)., Results: The AMA0428 dose-dependently reduced proliferation and VEGF-induced migration of HUVEC and HBMEC (P < 0.05). No significant effect was seen on HBVP proliferation; however, migration and pericyte recruitment were enhanced (P < 0.05) by AMA0428 administration. There was no apoptosis induction. The AMA0428 significantly reduced CNV and vessel leakage 2 weeks after laser treatment, comparable to DC101. In addition, AMA0428 inhibited inflammation on day 5 by 42% (P < 0.05) and collagen deposition on day 30 by 43% (P < 0. 05), whereas DC101 had no effect on inflammation nor on fibrosis., Conclusions: The results suggest that targeting ROCK with AMA0428 not only reduces neoangiogenesis, but also blocks inflammation and fibrosis (contrary to VEGF suppression). These results point to a potential therapeutic benefit of ROCK inhibition in neovascular AMD., (Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.)

Published
2015
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