1. In Vitro Evaluation of Hydroethanolic Extracts of Citrus Aurantium on Proliferative Rate of NSCs
- Author
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A.R. Abdanipour (PhD), B. Shadman, F. Salimi Nanehkaran, R. Bonabi, and A. Norian
- Subjects
citrus aurantium ,neural stem cells ,cell proliferation ,cell therapy. ,Medicine ,Medicine (General) ,R5-920 - Abstract
BACKGROUND AND OBJECTIVE: The use of an appropriate stimulus to speed up in vitro and in vivo stem cell proliferation and growth is the one of the most important issues in cell therapy. The aim of this study was in vitro evaluation of hydroethanolic extraction of citrus aurantium on proliferation and growth rates of neonate rat neural stem cells. METHODS: Neural stem cells were isolated using enzymatic digestion from hippocampus region of 3 day old neonatal rat brain. To determine optimal concentration of citrus aurantium extraction, isolated neural stem cells were treated at 200, 400, 600, 800 and 1000 µg/ml for 48 h. Then cells proliferation rate were evaluated using MTT assay method. FINDINGS: Citrus aurantium promoted neural stem cells proliferation compared to untreated cells. As well as the effect of aurantium extract is dose dependent. The result of this study showed that proliferative rate in treated cells with 600 µg/ml of Citrus aurantium extraction (0.81±0.06) significantly is higher than control (untreated neural stem cells) group (0.59±0.02). (p
- Published
- 2004