Background: This study reports a case of autologous tenon capsule packing to treat the posterior exit wound of penetrating injury., Case Summary: To treat a 58-year-old male patient with penetrating eyeball injury caused by an iron sheet, we used autologous tenon capsule packing. Two months after removal of the silicone oil, the corrected visual acuity was 0.3, the retina was flat, the tenon capsule graft was in place, the posterior wound closed well, and the intraocular pressure was 15.8 mmHg., Conclusion: Autologous tenon capsule packing to treat the posterior exit wound of penetrating injury is safe and effective., Competing Interests: Conflict-of-interest statement: All authors declare that they have no conflicts of interest to disclose., (©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.)