1. Investigation of T-cell receptor-γ gene rearrangement in gastrointestinal lymphomas by PCR-SSCP analysis
- Author
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Lan-Ying Shong, Tong Zhao, Hui-Yong Jiang, Xi-Qun Han, Li He, and Mei-Gang Zhu
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,Lymphoma ,Molecular Sequence Data ,Biology ,Polymerase Chain Reaction ,law.invention ,Pcr sscp ,law ,immune system diseases ,hemic and lymphatic diseases ,medicine ,Humans ,Base sequence ,Polymerase chain reaction ,Polymorphism, Single-Stranded Conformational ,Gastrointestinal Neoplasms ,Genetics ,Base Sequence ,Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor ,Gastroenterology ,T-Cell Receptor Gamma Gene ,General Medicine ,Gene rearrangement ,medicine.disease ,Molecular biology ,eye diseases ,Basic Research - Abstract
To analyze the characterization of T-cell receptor-gamma (TCR-gamma) gene rearrangement in the gastrointestinal lymphomas and evaluate the value of PCR-SSCP analysis in gastrointestinal lymphomas investigation.TCR-gamma gene rearrangement segments of gastrointestinal lymphomas were cloned and sequenced. Single clone plasmid and mixed clone plasmids were subsequently submitted to PCR-SSCP analysis to investigate the relationship between the number of amplified clones and band patterns of the amplified products. The PCR products of TCR-gamma gene rearrangement of 40 gastrointestinal lymphomas were electrophoresed on agarose gels and the positive cases on agarose gels were studied by SSCP analysis.The sequencing showed that TCR-gamma gene rearrangement of the gastrointestinal lymphomas included functional gene and pseudogene with extensive variety in the junctional regions. In SSCP analysis, the number of the single-stranded bands was about two times of the number of amplified clones, and double-stranded band became broad with the increased number of the amplified clones. Thirteen of the 25 B-cell gastrointestinal lymphomas and 14 of the 15 gastrointestinal T-cell lymphomas were positive detected on agarose gel electrophoresis. Of the positive cases detected by SSCP analysis, 3 B-cell lymphomas and 13 T-cell lymphomas showed positive bands. The other cases showed only smears. The rearranged pattern included 13 monoallelic gene rearrangements and 3 biallelic or oligoclonal gene rearrangements.The pattern of TCR-gamma gene rearrangement in gastrointestinal lymphomas are similar to that of the nodular lymphomas. PCR-SSCP analysis for TCR-gamma gene rearrangement can be applied both for adjuvant diagnosis of gastrointestinal lymphomas and analysis of the gene rearrangement pattern. The ratio of TCR-gamma gene rearrangements occurred in T-cell gastrointestinal lymphomas is significantly higher than that in B-cell gastrointestinal lymphomas. The gene rearrangement pattern involves monoallelic and biallelic (or oligoclonal) gene rearrangement.
- Published
- 2004