1. Expression of 12-lipoxygenase and its inhibitor's effect on proliferation of human AGS cell line
- Author
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Cai-Yun Huang, Feng-Lin Chen, Jian-Ying Li, Zhi-Xin Chen, and Xiao-Zhong Wang
- Subjects
Reverse transcription polymerase chain reaction ,chemistry.chemical_compound ,Lipoxygenase ,Messenger RNA ,TUNEL assay ,chemistry ,biology ,Apoptosis ,Cell culture ,biology.protein ,Molecular biology ,Fetal bovine serum ,Baicalein - Abstract
AIM: To investigate the expression of 12-lipoxygenase (12LOX) and the possible effect of its inhibitor on proliferation of human gastric cancer cell line AGS. METHODS: AGS cells were cultured in RPMI-1640 medium with 100 mL/L fetal bovine serum (FBS), 100 kU/L penicillin and 100 kU/L streptomycin. 12-LOX mRNA was analyzed by reverse transcription polymerase chain reaction (RT-PCR). The effect of baicalein on the proliferation of the cells was detected by thiazolyl blue tetrazolium bromide (MTT) method. The morphological changes of cellular and subcellullar structures were observed under inverted microscope and electron microscopy. AO-EB double and TUNEL staining were used to evaluate the apoptotic index of AGS cells.
- Published
- 2005
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