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The objective of the present study was to investigate the anti-cancer effects of eriodictyol in human hepatocellular carcinoma cells (Hep-G2) and normal liver hepatocyte cell line (AML12) along with evaluating its mode of action. Sulforhodamine B assay was used to evaluate the cytotoxic effect of the compound while as fluorescence microscopy was involved to demonstrate the effect of eriodictyol on cellular apoptosis. Flow cytometry was used to investigate the effect of eriodictyol on cell cycle while Western blot analysis revealed the effect on apoptosis-related protein expressions. Results indicate that eriodictyol-induced selective and concentration-dependent cytotoxic effect on Hep-G2 cancer cells while AML12 normal liver cells were very less susceptible to its effect. Eriodictyol-induced apoptosis related morphological changes including chromatin condensation and nuclear fragmentation. It also induced G2/M cell cycle arrest in these cells. Eriodictyol led to up-regulation of Bax and PARP and down-regulation of Bcl-2 protein. Video Clip of Methodology: Flow cytometry assay: 35 sec Full Screen Alternate

In the present study, effect of retinoic acid ortho-hydroxy aniline amide (RAA), an analog of retinoic acid (RA) was investigated on neurotrophin mediated cell growth and proliferation in hippocampus-derived stem cell clones. The results revealed that retinoic acid ortho-hydroxy aniline amide enhanced neuronal differentiation, increased NeuroD expression and p21 compared to proliferating cells stimulated by FGF-2. Retinoic acid ortho-hydroxy aniline amide treatment prevented decrease in RNA for trkC, enhanced expression of trkB RNA and p75NGFR. Retinoic acid ortho-hydroxy aniline amide treatment for 5 days increased the baseline expression of c-fos mRNA following stimulation with NGF, BDNF, and NT-3. Treatment of cultures with retinoic acid ortho-hydroxy aniline amide for 5 days followed by 5 days in serum alone increased Map2ab-positive cells compared to that of control. Therefore, retinoic acid ortho-hydroxy aniline amide is an effective agent for inducing neuronal differentiation and proliferation and is even more potent than the parent retinoic acid.

In the present study, effect of retinoic acid ortho-hydroxyaniline amide (RAA), an analog of retinoic acid (RA) was investigated on neurotrophin mediated cell growth and proliferation in hippocampus-derived stem cell clones. The results revealed that retinoic acid ortho-hydroxy aniline amide enhanced neuronal differentiation, increased NeuroD expression and p21 compared to proliferating cells stimulated by FGF-2. Retinoic acid ortho-hydroxyaniline amide treatment prevented decrease in RNA for trkC, enhanced expression of trkB RNA and p75NGFR. Retinoic acid ortho-hydroxyaniline amide treatment for 5 days increased the baseline expression of c-fos mRNA following stimulation with NGF, BDNF, and NT-3. Treatment of cultures with retinoic acid ortho-hydroxyaniline amide for 5 days followed by 5 days in serum alone increased Map2ab-positive cells compared to that of control. Therefore, retinoic acid ortho-hydroxyaniline amide is an effective agent for inducing neuronal differentiation and proliferation and is even more potent than the parent retinoic acid. [ABSTRACT FROM AUTHOR]