1. Expression of the BMP 2 Gene during Bone Cell Differentiation
- Author
-
Stephen E. Harris, Nandini Ghosh-Choudhury, Gregory R. Mundy, Jian Q. Feng, and Marie A. Harris
- Subjects
Mice, Transgenic ,Regulatory Sequences, Nucleic Acid ,Biology ,Bone morphogenetic protein ,Bone morphogenetic protein 2 ,Bone and Bones ,Mice ,Transforming Growth Factor beta ,Bone cell ,TGF beta signaling pathway ,Genetics ,Animals ,Molecular Biology ,Osteoblasts ,Helix-Loop-Helix Motifs ,Gene Expression Regulation, Developmental ,Proteins ,Bone morphogenetic protein 10 ,Cell Differentiation ,Molecular biology ,Rats ,BMPR2 ,Cell biology ,Bone morphogenetic protein 7 ,Transforming growth factor, beta 3 ,Bone Morphogenetic Proteins ,Transcription Factors - Abstract
Bone morphogenetic protein 2 (BMP 2) and transforming growth factor beta (TGF beta) are actively involved in bone formation and remodeling. TGF beta, a powerful stimulant in the early stage of bone cell growth and matrix formation, inhibits differentiation and in vitro mineralized nodule formation in primary fetal rat calvarial osteoblast system. TGF beta also negatively regulates BMP 2 expression at the transcriptional level. BMP 2 gene expression is controlled by a battery of transcriptional factors, some known and some yet to be identified. Immortalized osteoblast cell lines generated from a transgenic mouse carrying BMP 2 promoter-driven SV40 large T antigen transgene are described as powerful tools for studying regulation of BMP 2 gene expression and bone cell differentiation at the molecular level.
- Published
- 1994
- Full Text
- View/download PDF