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We established an animal model of radiation-induced skin ulcers in rats irradiated with 35-55Gy gamma-rays. The pathological changes were studied for 1 year. Immunohistochemical studies were performed on 72 radiation-induced skin ulcer specimens using c-erbB-2 and epidermal growth factor receptor (EGF-R) protein antibodies. We found that the overexpression rate of c-erbB2 oncoprotein was 59.7% and of EGF-R was 70.8%. The overexpression of c-erbB-2 was chiefly seen in the activated squamous epithelial cells and in the cytoplasm of fibroblasts, endothelial cells, and leiomyocytes of the arteriolar media in the deep part of the ulcers. The overexpression of EGF-R had the same localizations. It is suggested that the overexpression of c-erbB-2 and EGF-R proteins may be due to the poor healing of the radiation-induced skin ulcers.

We established an animal model of radiation-induced skin ulcers in rats irradiated with 35 to 55 Gy y-rays. The pathological changes were observed for 1 year. Immunohistochemical studies were performed on 72 radiation-induced skin ulcer specimens using c-fos and Rb protein polyclonal antibodies. We found that the overexpression rate of c-fos was 45.8% and of Rb was 63.9%. The overexpression of c-fos was mainly seen in the nuclei of activated squamous epithelial cells and in some fibroblasts and endothelial cells of arterioles in the deep part of the skin ulcers. The overexpression of Rb had the same localization. Our results suggest that the changes in c-fos and Rb proteins may be related to the poor healing of radiation-induced skin ulcers.

We established an animal model of radiation-induced skin ulcer in rats that were locally irradiated with 35 to 55 Gy gamma-rays. The pathological changes were observed for 1 year. Immunohistochemical studies using p53 and MDM2 protein polyclonal antibodies were performed on 72 radiation-induced skin ulcer specimens. The results showed that the overexpression rate of p53 protein was 9.7% and of MDM2 was 19.4%. The overexpression of p53 was chiefly seen in the nuclei of the activated squamous epithelial cells and in the fibroblasts and endothelial cells of arterioles in the deeper part of the skin ulcers. The overexpression of MDM2 had the same localizations. These results suggest that the changes of p53 and MDM2 may be related to the poor healing rate of radiation-induced skin ulcers.

Using immunohistochemistry, we studied the overexpression of MDM2, p53, and NCAM proteins in human radiation-induced skin ulcers. We found that the positive rate of overexpression of MDM2, p53, and NCAM was 36%, 8%, and 32%, respectively. The overexpression of MDM2 protein was mainly observed in the nuclei of fibroblasts in the deeper part of the ulcer; that of p53 protein was in the nuclei of the epidermis and in the cytoplasm of fibroblasts and endothelial cells, whereas that of NCAM was located in the cytoplasm of squamous epithelial cells of the epidermis and in fibroblasts, fibrocytes, endothelial cells, and leiomyocytes in the media of arteriolar walls. The overexpression of MDM2, p53, and NCAM may be related to the poor healing of radiation-induced skin ulcers and the cancer transformation.

We studied the overexpression of c-erbB-2 and p21 oncoproteins in human radiation-induced skin ulcers using immunohistochemistry. We found that the positive rate of overexpression of c-erbB-2 and p21 oncoproteins was 92.0 and 92.9%, respectively. The overexpression of c-erbB-2 oncoprotein was observed mainly in the cell membrane of squamous epithelial cells and in the cytoplasm of fibroblasts, endothelial cells, leiomyocytes in the media, and in fibrocytes of the adventitia of mesenchymal arterioles. The location of the p21 oncoprotein overexpression was mostly similar to that of c-erbB-2 with stronger staining in the cytoplasm of squamous epithelial cells and weaker staining in mesenchymal arteriolar walls. The overexpression of c-erbB-2 and p21 oncoproteins may be corresponding to the cancer transformation and poor healing of radiation-induced skin ulcers.