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This work was planned to investigate the prevalence of antimicrobial resistance of Enterococcus faecalis isolated from animal and human sources. Ten isolates of E. faecalis recovered from urinary tract infections in humans, as well as, ten isolates of E. faecalis were recovered from diarrheic dairy cattle studied for their antimicrobial sensitivity to 7 different antibacterial agents. Antimicrobial sensitivity pattern proved that most isolates were resistant to most of the tested antimicrobial agents. All isolates of human E. faecalis were 100 % resistant to rifamycin, gentamicin and penicillin G. Resistance to amikacin, ciprofloxacin, levofloxacin and vancomycin was 80.0%, 90.0%, 90.0% and 70.0% respectively. However animal E. faecalis were completely (100%) resistant to penicillin G and ciprofloxacin. Resistance to gentamicin, amikacin, levofloxacin, rifamycin and vancomycin was 70.0%, 40.0%, 20.0%, 20.0% and 0.0% respectively. PCR was applied on MDR for detection of aminoglycosides resistance genes. All human E. faecalis isolates were negative for aph(2")-Ia, aph(2")-Ib, aph(2")-Ic and aph(2")-Id. 40.0% of isolates were proved to harbour aph(3")-IIIa and 10.0% (one isolate) harboured ant(4")-Ia. However all animal E. faecalis isolates were negative for aph (2")-Ib, aph(2")-Ic, aph(2")-Id. Two isolates (20.0%) harboured aph(2")-Ia and ant(4")-Ia and four isolates (40.0%) harboured aph(3")-IIIa. In conclusion, the increased antibiotic resistance of E. faecalis isolated from animal and human sources complicate treatment decisions and increase public health hazard. [ABSTRACT FROM AUTHOR]