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1. Cloning and characterization of a cDNA encoding a cowpea seed defensin and analysis of its expression.

Author

Carvalho AO, Filho GA, Ferreira BS, Branco AT, Okorokova-Façanha AL, and Gomes VM

Subjects
Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Complementary genetics, Defensins biosynthesis, Fabaceae chemistry, Fabaceae metabolism, Molecular Sequence Data, Defensins chemistry, Defensins genetics, Fabaceae genetics, Seeds genetics, Seeds metabolism
Abstract

We have previously isolated a cowpea seed defensin with potent antifungal activity. Here we report the cloning of its cDNA obtained from RT-PCR. The sequence of the deduced protein VUDEF exhibited homology to plant defensins. Northern analysis revealed that VUDEF mRNA is accumulated during cowpea seed development and its level increases in seedling tissues after exposure to fungal pathogen and cold.

Published
2006
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