1. TiO2~Deoxyribozyme Nanocomposites as Delivery System and Efficient Site-Specific Agents for Cleavage of RNA Targets
- Author
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V. F. Zarytova, Asya S. Levina, Zinfer R. Ismagilov, Marina Repkova, Natalia V. Shatskaya, and Nadezhda V. Shikina
- Subjects
Materials science ,technology, industry, and agriculture ,Biomedical Engineering ,Deoxyribozyme ,Pharmaceutical Science ,RNA ,Nanoparticle ,Nanotechnology ,Cleavage (embryo) ,Combinatorial chemistry ,chemistry.chemical_compound ,chemistry ,Cleave ,Polylysine ,RNA Cleavage ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Linker - Abstract
Synthetic DNA molecules of 10-23 deoxyribozymes (Dz) are known to be efficient and site-specific agents for the RNA cleavage in a catalytic manner. To provide the penetration of Dz into cells, we have proposed the new delivery system for deoxyribozymes in the form of TiO 2 •PL-Dz nanocomposite. These nanocomposites consist of catalytically ac- tive Dz molecules noncovalently immobilized through the polylysine linker to TiO 2 nanoparticles, which provide the penetration of Dz into cells. Deoxyribozymes in the prepared TiO 2 •PL-Dz nanocomposites were shown to retain their ability to cleave RNA targets albeit at a slower rate but with the same site-specificity and similar efficiency as free Dz. Unlike free Dz, the proposed TiO 2 •PL-Dz nanocomposites penetrate into cells without auxiliary actions.
- Published
- 2014
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