1. Plant Assays for Quantifying Ralstonia solanacearum Virulence
- Author
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Caitilyn Allen, Tuan Minh Tran, Tiffany M. Lowe-Power, and Devanshi Khokhani
- Subjects
0301 basic medicine ,Microbial pathogenesis ,Ralstonia solanacearum ,biology ,Strategy and Management ,Mechanical Engineering ,Bacterial wilt ,fungi ,030106 microbiology ,Metals and Alloys ,food and beverages ,Mutagenesis (molecular biology technique) ,Virulence ,biology.organism_classification ,Industrial and Manufacturing Engineering ,Microbiology ,03 medical and health sciences ,030104 developmental biology ,In vivo ,Pathogen ,Bacteria - Abstract
Virulence assays are powerful tools to study microbial pathogenesis in vivo. Good assays track disease development and, coupled with targeted mutagenesis, can identify pathogen virulence factors. Disease development in plants is extremely sensitive to environmental factors such as temperature, atmospheric humidity, and soil water level, so it can be challenging to standardize conditions to achieve consistent results. Here, we present optimized and validated experimental conditions and analysis methods for nine assays that measure specific aspects of virulence in the phytopathogenic bacterium Ralstonia solanacearum, using tomato as the model host plant.
- Published
- 2018
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