29 results on '"Shen, Qian"'
Search Results
2. Acellular dermal matrix for one-stage treatment of lower extremity full-thickness skin defect: a case series
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Li, Gongchi, Shen, Qian, Zhou, Pan, Liu, Hanlin, and Chen, Jianghai
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- 2023
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3. CXCR6-positive circulating mucosal-associated invariant T cells can identify patients with non-small cell lung cancer responding to anti-PD-1 immunotherapy
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Qu, Jingjing, Wu, Binggen, Chen, Lijun, Wen, Zuoshi, Fang, Liangjie, Zheng, Jing, Shen, Qian, Heng, Jianfu, Zhou, Jianya, and Zhou, Jianying
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- 2024
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4. GEN1 as a risk factor for human congenital anomalies of the kidney and urinary tract
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Du, Xuanjin, Wang, Chunyan, Liu, Jialu, Yu, Minghui, Ju, Haixin, Xue, Shanshan, Li, Yaxin, Liu, Jiaojiao, Dai, Rufeng, Chen, Jing, Zhai, Yihui, Rao, Jia, Wang, Xiang, Sun, Yubo, Sun, Lei, Wu, Xiaohui, Xu, Hong, and Shen, Qian
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- 2024
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5. Lymph node ratio is a prospective prognostic indicator for locally advanced gastric cancer patients after neoadjuvant chemotherapy
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Jiang, Qi, Zeng, Xiangyu, Zhang, Chenggang, Yang, Ming, Fan, Jun, Mao, Gan, Shen, Qian, Yin, Yuping, Liu, Weizhen, Tao, Kaixiong, and Zhang, Peng
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- 2022
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6. Comparative proteomic analysis of children FSGS FFPE tissues
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Ni, Jiajia, Tian, Sha, Bai, Lin, Lv, Qianying, Liu, Jialu, Liu, Jiaojiao, Fang, Ye, Zhai, Yihui, Shen, Qian, Rao, Jia, Ding, Chen, and Xu, Hong
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- 2022
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7. Targeting MUS81 promotes the anticancer effect of WEE1 inhibitor and immune checkpoint blocking combination therapy via activating cGAS/STING signaling in gastric cancer cells
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Li, Chengguo, Shen, Qian, Zhang, Peng, Wang, Tao, Liu, Weizhen, Li, Ruidong, Ma, Xianxiong, Zeng, Xiangyu, Yin, Yuping, and Tao, Kaixiong
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- 2021
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8. Genome-wide characterization of the WAK gene family and expression analysis under plant hormone treatment in cotton
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Dou, Lingling, Li, Zhifang, Shen, Qian, Shi, Huiran, Li, Huaizhu, Wang, Wenbo, Zou, Changsong, Shang, Haihong, Li, Hongbin, and Xiao, Guanghui
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- 2021
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9. The barley powdery mildew effectors CSEP0139 and CSEP0182 suppress cell death and promote B. graminis fungal virulence in plants
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Li, Xue, Jin, Cong, Yuan, Hongbo, Huang, Wanting, Liu, Fang, Fan, Renchun, Xie, Jiankun, and Shen, Qian-Hua
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- 2021
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10. Multidisciplinary approach to screening and management of children with Fabry disease: practice at a Tertiary Children’s Hospital in China
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Shen, Qian, Liu, Jialu, Chen, Jing, Zhou, Shuizheng, Wang, Yi, Yu, Lifei, Sun, Li, Wang, Liuhui, Wu, Bingbing, Liu, Fang, Cao, Yun, Huang, Ying, Wang, Jianshe, Yang, Chenhao, Zhu, Daqian, Ma, Yangyang, Xu, Zhengmin, Lu, Wei, Fu, Lili, Zhou, Wenhao, and Xu, Hong
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- 2021
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11. Phenotypic spectrum and genetics of PAX2-related disorder in the Chinese cohort
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Yang, Xue, Li, Yaqi, Fang, Ye, Shi, Hua, Xiang, Tianchao, Liu, Jiaojiao, Liu, Jialu, Tang, Xiaoshan, Fang, Xiaoyan, Chen, Jing, Zhai, Yihui, Shen, Qian, Bi, Yunli, Qian, Yanyan, Wu, Bingbing, Wang, Huijun, Zhou, Wenhao, Ma, Duan, Bai, Haitao, Mao, Jianhua, Chen, Lizhi, Wang, Xiaowen, Gao, Xiaojie, Zhang, Ruifeng, Zhuang, Jieqiu, Zhang, Aihua, Jiang, Xiaoyun, Xu, Hong, and Rao, Jia
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- 2021
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12. Money matters: a multicenter cross-sectional study of depressive symptoms among the caregivers of children on peritoneal dialysis in Mainland China
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Zhao, Rui, Gu, Ying, Shen, Xia, Mai, Xianying, Zhou, Cheng, Zhang, Yufen, Zhai, Yihui, Shen, Qian, Xu, Hong, and Zhou, Qing
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- 2020
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13. Molecular features of lung adenocarcinoma in young patients
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Chen, Zhen, Teng, Xiao, Zhang, Jing, Huang, Ke, Shen, Qian, Cao, He, Luo, Huisong, Yuan, Yanting, and Teng, Xiaodong
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- 2019
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14. Angiopoietin-like-3 knockout protects against glomerulosclerosis in murine adriamycin-induced nephropathy by attenuating podocyte loss
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Dai, Rufeng, Liu, Haimei, Han, Xinli, Liu, Junchao, Zhai, Yihui, Rao, Jia, Shen, Qian, and Xu, Hong
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- 2019
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15. HNF4A-related Fanconi syndrome in a Chinese patient: a case report and review of the literature
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Liu, Jiaojiao, Shen, Qian, Li, Guomin, and Xu, Hong
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- 2018
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16. pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor
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Zhai, Yihui, Xu, Hong, Shen, Qian, Schaefer, Franz, Schmitt, Claus P., Chen, Jing, Liu, Haimei, Liu, Jialu, and Liu, Jiaojiao
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- 2018
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17. Genome-wide characterization of the WAK gene family and expression analysis under plant hormone treatment in cotton
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Haihong Shang, Hongbin Li, Guanghui Xiao, Shen Qian, Zhifang Li, Huiran Shi, Lingling Dou, Huaizhu Li, Wenbo Wang, and Changsong Zou
- Subjects
0106 biological sciences ,lcsh:QH426-470 ,lcsh:Biotechnology ,Gossypium hirsutum ,Biology ,01 natural sciences ,Genome ,Expression analysis ,Transcriptome ,03 medical and health sciences ,Plant Growth Regulators ,Auxin ,Gene Expression Regulation, Plant ,lcsh:TP248.13-248.65 ,Arabidopsis ,Genetics ,Gene family ,Cotton Fiber ,Gene ,Phylogeny ,030304 developmental biology ,Synteny ,Plant Proteins ,chemistry.chemical_classification ,0303 health sciences ,Gossypium ,food and beverages ,biology.organism_classification ,lcsh:Genetics ,chemistry ,Fiber cell ,Multigene Family ,WAK genes ,010606 plant biology & botany ,Biotechnology ,Research Article - Abstract
Background Wall-associated kinases (WAK), one of the receptor-like kinases (RLK), function directly in the connection and communication between the plant cell wall and the cytoplasm. WAK genes are highly conserved and have been identified in plants, such as rice, but there is little research on the WAK gene family in cotton. Results In the present study, we identified 29 GhWAK genes in Gossypium hirsutum. Phylogenetic analysis showed that cotton WAK proteins can be divided into five clades. The results of synteny and Ka/Ks analysis showed that the GhWAK genes mainly originated from whole genome duplication (WGD) and were then mainly under purifying selection. Transcriptome data and real-time PCR showed that 97% of GhWAK genes highly expressed in cotton fibers and ovules. β-glucuronidase (GUS) staining assays showed that GhWAK5 and GhWAK16 expressed in Arabidopsis leaf trichomes. Fourteen GhWAK genes were found to possess putative gibberellin (GA) response elements in the promoter regions, 13 of which were significantly induced by GA treatment. Ten GhWAK genes contained auxin (IAA) response elements and the expression level of nine GhWAKs significantly increased under auxin treatment. Conclusions We provide a preliminary analysis of the WAK gene family in G. hirsutum, which sheds light on the potantial roles of GhWAK genes in cotton fiber cell development. Our data also provides a useful resource for future studies on the functional roles of GhWAK genes.
- Published
- 2021
18. Crizotinib in patients with anaplastic lymphoma kinase-positive advanced non-small cell lung cancer versus chemotherapy as a first-line treatment.
- Author
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Zhou, Jianya, Zheng, Jing, Zhang, Xiaochen, Zhao, Jing, Zhu, Yanping, Shen, Qian, Wang, Yuehong, Sun, Ke, Zhang, Zeying, Pan, Zhijie, Shen, Yihong, and Zhou, Jianying
- Subjects
NON-small-cell lung carcinoma ,CANCER chemotherapy ,CRIZOTINIB ,KAPLAN-Meier estimator ,PROPORTIONAL hazards models ,THERAPEUTICS - Abstract
Background: To compare the efficacy of crizotinib, pemetrexed and other chemotherapy regimens as a first-line treatment in patients with anaplastic lymphoma kinase (ALK)-positive non-small cell lung cancer (NSCLC) in real world clinical use and to evaluate the +86-571-87,236,876 predictive clinical factors of the efficacy of crizotinib.Methods: The 73 patients with ALK-positive advanced NSCLC were divided into three groups based on the first-line treatment: first-line crizotinib group (1-CRZ group, n = 32); first-line platinum-based pemetrexed treatment group (1-PP group, n = 28), and first-line chemotherapy platinum-based non-pemetrexed group (N1-PP, n = 12). Sixty eight of the 73 patients received crizotinib treatment and followed up in our hospital. Differences in the objective response rate (ORR), disease control rate (DCR) and progression-free survival (PFS) were compared in the different groups. The clinical factors were evaluated to predict the efficacy of crizotinib by the Kaplan-Meier survival analysis and Cox proportional hazards model.Results: The PFS, ORR, DCR were 16.1 months, 78.1% (25/32) and 100% (32/32) in the 1-CRZ group; were 6.0 months, 17.9% (5/28) and 57.2% (16/28) in the 1-PP group; and were 2.9 months, 15.4% (2/13) and 46.2% (6/13) in the N1-PP group. The PFS of the 1-CRZ group was significantly longer than that of the 1-PP group (P < 0.001) and the N1-PP group (P < 0.001). The ORR and DCR of the 1-CRZ group was significantly greater than that of the 1-PP group and the N1-PP group (all the P < 0.001). Higher Eastern Cooperative Oncology Group (ECOG) performance status score (> = 2) (HR 2.345, 95% CI 1.137-4.834, P = 0.021) and patients received crizotinib after N1-PP chemotherapy (HR 2.345, 95% CI 1.137-4.834, P = 0.021) were two factors associated with shorter PFS after crizotinib treatment.Conclusions: In patients with ALK-positive NSCLC who did not receive previous treatment, crizotinib was superior to standard chemotherapy for the longer PFS and greater ORR and DCR. Higher ECOG score (> = 2) and patients received crizotinib after N1-PP chemotherapy predict poor efficacy of crizotinib. [ABSTRACT FROM AUTHOR]- Published
- 2018
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19. New congenital anomalies of the kidney and urinary tract and outcomes in Robo2 mutant mice with the inserted piggyBac transposon.
- Author
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Jialu Liu, Li Sun, Qian Shen, Xiaohui Wu, Hong Xu, Liu, Jialu, Sun, Li, Shen, Qian, Wu, Xiaohui, and Xu, Hong
- Subjects
CONGENITAL disorders ,KIDNEY diseases ,URINARY tract infections ,GENE expression ,VESICO-ureteral reflux ,DISEASE incidence ,LABORATORY mice - Abstract
Background: Disruption of ROBO2 in humans causes vesicoureteral reflux (VUR)/congenital anomalies of the kidney and urinary tract (CAKUT). PiggyBac (PB) is a DNA transposon, and its insertion often reduces-but does not eliminate-gene expression. The Robo2 insertion mutant exhibited non-dilating VUR, ureteropelvic junction obstruction (UPJO) not found in reported models. We studied the incidence and outcomes of VUR/CAKUT in this mutant and explored the relationship between Robo2 gene expression and the occurrence and severity of VUR/CAKUT.Methods: The urinary systems of newborn mutants were evaluated via Vevo 770 micro-ultrasound. Some of the normal animals-and all of the abnormal animals-were followed to adulthood and tested for VUR. Urinary obstruction experiments were performed on mice with hydronephrosis. The histology of the kidney and ureter was examined by light microscopy and transmission electron microscopy. Robo2 (PB/PB) mice were crossed with Hoxb7/myr-Venus mice to visualize the location of the ureters relative to the bladder.Results: In Robo2 (PB/PB) mice, PB insertion led to an approximately 50 % decrease in Robo2 gene expression. The most common (27.07 %, 62/229) abnormality was non-dilating VUR, and no statistically significant differences were found between age groups. Approximately 6.97 % displayed ultrasound-detectable CAKUT, and these mice survived to adulthood without improvement. No severe CAKUT were found in Robo2 (PB/+) mice. The refluxing ureters showed disorganized smooth muscle fibers, reduced muscle cell populations, intercellular edema and intracytoplasmic vacuoles in smooth muscle cells. Both UPJ and UVJ muscle defects were noted in Robo2 (PB/PB) mice.Conclusions: Robo2 (PB/PB) mice is the first Robo2-deficient mouse model to survive to adulthood while displaying non-dilating VUR, UPJO, and multiple ureters with blind endings. The genetic background of these mutants may influence the penetrance and severity of the CAKUT phenotypes. VUR and other CAKUT found in this mutant had little chance of spontaneous resolution, and this requires careful follow-up. We reported for the first time that the non-dilated refluxing ureters showed disorganized smooth muscle fibers and altered smooth muscle cell structure, more accurately mimicking the characteristics of human cases. Future studies are required to test the role of Robo2 in the ureteric smooth muscle. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
20. Hospital pharmacists' knowledge of and attitudes towards the implementation of the National Essential Medicines System: a questionnaire survey in western China.
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Qian Shen, Caijun Yang, Jie Chang, Lina Wu, Wenwen Zhu, Bing Lv, Dan Ye, Shimin Yang, Yu Fang, Shen, Qian, Yang, Caijun, Chang, Jie, Wu, Lina, Zhu, Wenwen, Lv, Bing, Ye, Dan, Yang, Shimin, and Fang, Yu
- Subjects
HEALTH care reform ,PHARMACISTS ,IMPLEMENTATION (Social action programs) ,PUBLIC hospitals ,THEORY of knowledge ,ATTITUDE (Psychology) ,HEALTH attitudes ,HOSPITAL pharmacies ,MEDICAL personnel ,PRIMARY health care ,ESSENTIAL drugs ,PSYCHOLOGY - Abstract
Background: In 2009, Chinese government launched a new healthcare reform, one of the key points of which is to establish National Essential Medicine System (NEMS). Hospital pharmacists are directly related to the implementation of NEMS. This study is to examine knowledge of and attitudes towards the implementation of the NEMS among hospital pharmacists in western China.Methods: We conducted a questionnaire survey of pharmacists from different types of medical institutions in Shaanxi Province in November 2014. We gathered demographic information about the participants, collected the data about their knowledge of and attitudes towards the implementation of NEMS, and identified the influencing factors of cognitive level. We analyzed the data and compared public secondary/tertiary hospitals and primary healthcare institutions.Results: Of the 704 participants (response rate = 70.2 %), the majority had positive and moderate knowledge (39.2 and 53.3 %) and attitudes (35.8 and 62.9 %) towards NEMS. The most participants were aware of the implementation time of NEMS (89.8 %) and zero mark-up policy (85.5 %) while the least learned of the adjustment time of National Essential Medicines List (NEML). Pharmacists from public secondary/tertiary hospitals tended to know more and have more positive attitudes. There was no statistical correlation between knowledge and attitude scores. The education level (p = 0.022) and number of training sessions attended (p = 0.028) were the only demographic variables linked to knowledge scores.Conclusions: Hospital pharmacists in Shaanxi Province had moderate knowledge of and attitudes towards the implementation of NEMS. Pharmacists from public secondary/tertiary hospitals showed better understanding. The government should therefore focus on improving the understanding of pharmacists in primary healthcare institutions and also address existing problems, especially the supply and distribution systems. [ABSTRACT FROM AUTHOR]- Published
- 2016
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21. Identification and characterization of microRNAs related to salt stress in broccoli, using high-throughput sequencing and bioinformatics analysis.
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Yunhong Tian, Yunming Tian, Xiaojun Luo, Tao Zhou, Zuoping Huang, Ying Liu, Yihan Qiu, Bing Hou, Dan Sun, Hongyu Deng, Shen Qian, and Kaitai Yao
- Subjects
BROCCOLI ,MICRORNA ,SALT ,BIOINFORMATICS ,REVERSE transcriptase polymerase chain reaction - Abstract
Background MicroRNAs (miRNAs) are a new class of endogenous regulators of a broad range of physiological processes, which act by regulating gene expression post-transcriptionally. The brassica vegetable, broccoli (Brassica oleracea var. italica), is very popular with a wide range of consumers, but environmental stresses such as salinity are a problem worldwide in restricting its growth and yield. Little is known about the role of miRNAs in the response of broccoli to salt stress. In this study, broccoli subjected to salt stress and broccoli grown under control conditions were analyzed by high-throughput sequencing. Differential miRNA expression was confirmed by real-time reverse transcription polymerase chain reaction (RTPCR). The prediction of miRNA targets was undertaken using the Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology (KO) database and Gene Ontology (GO)-enrichment analyses. Results Two libraries of small (or short) RNAs (sRNAs) were constructed and sequenced by heighthroughput Solexa sequencing. A total of 24,511,963 and 21,034,728 clean reads, representing 9,861,236 (40.23%) and 8,574,665 (40.76%) unique reads, were obtained for control and salt-stressed broccoli, respectively. Furthermore, 42 putative known and 39 putative candidate miRNAs that were differentially expressed between control and saltstressed broccoli were revealed by their read counts and confirmed by the use of stem-loop real-time RT-PCR. Amongst these, the putative conserved miRNAs, miR393 and miR855, and two putative candidate miRNAs, miR3 and miR34, were the most strongly downregulated when broccoli was salt-stressed, whereas the putative conserved miRNA, miR396a, and the putative candidate miRNA, miR37, were the most up-regulated. Finally, analysis of the predicted gene targets of miRNAs using the GO and KO databases indicated that a range of metabolic and other cellular functions known to be associated with salt stress were upregulated in broccoli treated with salt. Conclusion A comprehensive study of broccoli miRNA in relation to salt stress has been performed. We report significant data on the miRNA profile of broccoli that will underpin further studies on stress responses in broccoli and related species. The differential regulation of miRNAs between control and salt-stressed broccoli indicates that miRNAs play an integral role in the regulation of responses to salt stress. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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22. Transcriptome analysis and identification of genes associated with fruiting branch internode elongation in upland cotton.
- Author
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Ju, Feiyan, Liu, Shaodong, Zhang, Siping, Ma, Huijuan, Chen, Jing, Ge, Changwei, Shen, Qian, Zhang, Xiaomeng, Zhao, Xinhua, Zhang, Yongjiang, and Pang, Chaoyou
- Subjects
COTTON quality ,COTTON ,JASMONATE ,GERMPLASM ,COTTON picking ,PLANT hormones ,COTTON growing ,AMINO acid synthesis - Abstract
Background: Appropriate plant architecture can improve the amount of cotton boll opening and allow increased planting density, thus increasing the level of cotton mechanical harvesting and cotton yields. The internodes of cotton fruiting branches are an important part of cotton plant architecture. Thus, studying the molecular mechanism of internode elongation in cotton fruiting branches is highly important. Results: In this study, we selected internodes of cotton fruiting branches at three different stages from two cultivars whose internode lengths differed significantly. A total of 76,331 genes were detected by transcriptome sequencing. By KEGG pathway analysis, we found that DEGs were significantly enriched in the plant hormone signal transduction pathway. The transcriptional data and qRT-PCR results showed that members of the GH3 gene family, which are involved in auxin signal transduction, and CKX enzymes, which can reduce the level of CKs, were highly expressed in the cultivar XLZ77, which has relatively short internodes. Genes related to ethylene synthase (ACS), EIN2/3 and ERF in the ethylene signal transduction pathway and genes related to JAR1, COI1 and MYC2 in the JA signal transduction pathway were also highly expressed in XLZ77. Plant hormone determination results showed that the IAA and CK contents significantly decreased in cultivar XLZ77 compared with those in cultivar L28, while the ACC (the precursor of ethylene) and JA contents significantly increased. GO enrichment analysis revealed that the GO categories associated with promoting cell elongation, such as cell division, the cell cycle process and cell wall organization, were significantly enriched, and related genes were highly expressed in L28. However, genes related to the sphingolipid metabolic process and lignin biosynthetic process, whose expression can affect cell elongation, were highly expressed in XLZ77. In addition, 2067 TFs were differentially expressed. The WRKY, ERF and bHLH TF families were the top three largest families whose members were active in the two varieties, and the expression levels of most of the genes encoding these TFs were upregulated in XLZ77. Conclusions: Auxin and CK are positive regulators of internode elongation in cotton branches. In contrast, ethylene and JA may act as negative regulators of internode elongation in cotton branches. Furthermore, the WRKY, ERF and bHLH TFs were identified as important inhibitors of internode elongation in cotton. In XLZ77(a short-internode variety), the mass synthesis of ethylene and amino acid conjugation of auxin led to the inhibition of plant cell elongation, while an increase in JA content and degradation of CKs led to a slow rate of cell division, which eventually resulted in a phenotype that presented relatively short internodes on the fruiting branches. The results of this study not only provide gene resources for the genetic improvement of cotton plant architecture but also lay a foundation for improved understanding of the molecular mechanism of the internode elongation of cotton branches. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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23. Gene mutation analysis in 12 Chinese children with congenital nephrotic syndrome.
- Author
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Li, Guo-min, Cao, Qi, Shen, Qian, Sun, Li, Zhai, Yi-hui, Liu, Hai-mei, An, Yu, and Xu, Hong
- Subjects
CONGENITAL disorders ,NEPHROTIC syndrome ,GENETIC mutation ,GENETIC disorders ,EXONS (Genetics) - Abstract
Background: Congenital nephrotic syndrome (CNS) is characterised by increased proteinuria, hypoproteinemia, and edema beginning in the first 3 months of life. Recently, molecular genetic studies have identified several genes involved in the pathogenesis of CNS. A systematic investigation of the genes for CNS in China has never been performed; therefore, we conducted a mutational analysis in 12 children with CNS,with the children coming from 10 provinces and autonomous regions in China.Methods: Twelve children with CNS were enrolled from 2009 to 2016. A mutational analysis was performed in six children by Sanger sequencing in eight genes (NPHS1, NPHS2, PLCE1, WT1, LAMB2, LMXIB, COQ6 and COQ2) before 2014, and whole-exome sequencing was used from 2014 to 2016 in another six children. Significant variants that were detected by next generation sequencing were confirmed by conventional Sanger sequencing in the patients' families.Results: Of the 12 children, eight patients had a compound heterozygous NPHS1 mutation, one patient had a de novo mutation in the WT1 gene, and another patient with extrarenal symptoms had a homozygous mutation in the COQ6 gene. No mutations were detected in genes NPHS2, PLCE1, LAMB2, LMXIB, and COQ2 in the 12 patients.Conclusions: This study demonstrates that the majority of CNS cases (67%, 8/12 patients) are caused by genetic defects, and the NPHS1 mutation is the most common cause of CNS in Chinese patients. A mutational analysis of NPHS1 should be recommended in Chinese patients with CNS in all exons of NPHS1 and in the intron-exon boundaries. [ABSTRACT FROM AUTHOR]- Published
- 2018
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24. pH-mediated upregulation of <italic>AQP1</italic> gene expression through the Spi-B transcription factor.
- Author
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Zhai, Yihui, Xu, Hong, Shen, Qian, Schaefer, Franz, Schmitt, Claus P., Chen, Jing, Liu, Haimei, Liu, Jialu, and Liu, Jiaojiao
- Subjects
AQUAPORINS ,PERITONEAL dialysis ,GENETIC transcription ,HYDROGEN-ion concentration ,CATALYST supports - Abstract
Background: Bicarbonate-based peritoneal dialysis (PD) fluids enhance the migratory capacity and damage-repair ability of human peritoneal mesothelial cells by upregulating AQP1. However, little is known about the underlying molecular mechanisms. Results: Here we used HEK-293T cells to investigate the effect of pH on
AQP1 gene transcription levels. We found thatAQP1 mRNA levels increases with pH. Transfection of HEK-293T cells with luciferase reporter vectors containing different regions of theAQP1 promoter identified an upstream region in theAQP1 gene between − 2200 and – 2300 bp as an enhancer required for pH-mediated regulation ofAQP1 expression. Site-directed mutagenesis of this specific promoter region revealed a critical region between − 2257 and − 2251 bp, and gene knock-down experiments and ChIP assays suggested that the Spi-B transcription factor SPIB is involved in pH-mediated regulation of AQP1 expression. Conclusions: We identified an upstream region in theAQP1 gene and the transcription factor SPIB that are critically involved in pH-mediated regulation of AQP1 expression. These findings provide the basis for further studies on the pH- and buffer-dependent effects of PD fluids on peritoneal membrane integrity and function. [ABSTRACT FROM AUTHOR]- Published
- 2018
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- View/download PDF
25. Mutational analysis of AGXT in two Chinese families with primary hyperoxaluria type 1.
- Author
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Li, Guo-Min, Xu, Hong, Shen, Qian, Gong, Yi-Nv, Fang, Xiao-Yan, Sun, Li, Liu, Hai-Mei, and An, Yu
- Abstract
Background: Primary hyperoxaluria type 1 is a rare autosomal recessive disease of glyoxylate metabolism caused by a defect in the liver-specific peroxisomal enzyme alanine:glyoxylate aminotransferase (AGT) that leads to hyperoxaluria, recurrent urolithiasis, and nephrocalcinosis.Methods: Two unrelated patients with recurrent urolithiasis, along with members of their families, exhibited mutations in the AGXT gene by PCR direct sequencing.Results: Two heterozygous mutations that predict truncated proteins, p.S81X and p.S275delinsRAfs, were identified in one patient. The p.S81X mutation is novel. Two heterozygous missense mutations, p.M1T and p.I202N, were detected in another patient but were not identified in her sibling. These four mutations were confirmed to be of paternal and maternal origin.Conclusions: These are the first cases of primary hyperoxaluria type 1 to be diagnosed by clinical manifestations and AGXT gene mutations in mainland China. The novel p.S81X and p.I202N mutations detected in our study extend the spectrum of known AGXT gene mutations. [ABSTRACT FROM AUTHOR]- Published
- 2014
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- View/download PDF
26. New congenital anomalies of the kidney and urinary tract and outcomes in Robo2 mutant mice with the inserted piggyBac transposon.
- Author
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Liu J, Sun L, Shen Q, Wu X, and Xu H
- Subjects
- Animals, Female, Heterozygote, Homeodomain Proteins genetics, Homozygote, Hydronephrosis genetics, Kidney diagnostic imaging, Kidney pathology, Male, Mice, Microscopy, Electron, Transmission, Microscopy, Fluorescence, Mutation, Transgenes, Ultrasonography, Ureter diagnostic imaging, Ureter pathology, Ureteral Obstruction diagnostic imaging, Ureteral Obstruction genetics, Urothelium pathology, Vesico-Ureteral Reflux diagnostic imaging, Vesico-Ureteral Reflux pathology, DNA Transposable Elements genetics, Kidney abnormalities, Receptors, Immunologic genetics, Ureter abnormalities, Vesico-Ureteral Reflux genetics
- Abstract
Background: Disruption of ROBO2 in humans causes vesicoureteral reflux (VUR)/congenital anomalies of the kidney and urinary tract (CAKUT). PiggyBac (PB) is a DNA transposon, and its insertion often reduces-but does not eliminate-gene expression. The Robo2 insertion mutant exhibited non-dilating VUR, ureteropelvic junction obstruction (UPJO) not found in reported models. We studied the incidence and outcomes of VUR/CAKUT in this mutant and explored the relationship between Robo2 gene expression and the occurrence and severity of VUR/CAKUT., Methods: The urinary systems of newborn mutants were evaluated via Vevo 770 micro-ultrasound. Some of the normal animals-and all of the abnormal animals-were followed to adulthood and tested for VUR. Urinary obstruction experiments were performed on mice with hydronephrosis. The histology of the kidney and ureter was examined by light microscopy and transmission electron microscopy. Robo2 (PB/PB) mice were crossed with Hoxb7/myr-Venus mice to visualize the location of the ureters relative to the bladder., Results: In Robo2 (PB/PB) mice, PB insertion led to an approximately 50 % decrease in Robo2 gene expression. The most common (27.07 %, 62/229) abnormality was non-dilating VUR, and no statistically significant differences were found between age groups. Approximately 6.97 % displayed ultrasound-detectable CAKUT, and these mice survived to adulthood without improvement. No severe CAKUT were found in Robo2 (PB/+) mice. The refluxing ureters showed disorganized smooth muscle fibers, reduced muscle cell populations, intercellular edema and intracytoplasmic vacuoles in smooth muscle cells. Both UPJ and UVJ muscle defects were noted in Robo2 (PB/PB) mice., Conclusions: Robo2 (PB/PB) mice is the first Robo2-deficient mouse model to survive to adulthood while displaying non-dilating VUR, UPJO, and multiple ureters with blind endings. The genetic background of these mutants may influence the penetrance and severity of the CAKUT phenotypes. VUR and other CAKUT found in this mutant had little chance of spontaneous resolution, and this requires careful follow-up. We reported for the first time that the non-dilated refluxing ureters showed disorganized smooth muscle fibers and altered smooth muscle cell structure, more accurately mimicking the characteristics of human cases. Future studies are required to test the role of Robo2 in the ureteric smooth muscle.
- Published
- 2016
- Full Text
- View/download PDF
27. Hospital pharmacists' knowledge of and attitudes towards the implementation of the National Essential Medicines System: a questionnaire survey in western China.
- Author
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Shen Q, Yang C, Chang J, Wu L, Zhu W, Lv B, Ye D, Yang S, and Fang Y
- Subjects
- Adult, China, Female, Health Care Reform, Hospitals, Public, Humans, Male, Middle Aged, Primary Health Care, Surveys and Questionnaires, Young Adult, Attitude of Health Personnel, Drugs, Essential, Health Knowledge, Attitudes, Practice, Pharmacists psychology, Pharmacy Service, Hospital
- Abstract
Background: In 2009, Chinese government launched a new healthcare reform, one of the key points of which is to establish National Essential Medicine System (NEMS). Hospital pharmacists are directly related to the implementation of NEMS. This study is to examine knowledge of and attitudes towards the implementation of the NEMS among hospital pharmacists in western China., Methods: We conducted a questionnaire survey of pharmacists from different types of medical institutions in Shaanxi Province in November 2014. We gathered demographic information about the participants, collected the data about their knowledge of and attitudes towards the implementation of NEMS, and identified the influencing factors of cognitive level. We analyzed the data and compared public secondary/tertiary hospitals and primary healthcare institutions., Results: Of the 704 participants (response rate = 70.2 %), the majority had positive and moderate knowledge (39.2 and 53.3 %) and attitudes (35.8 and 62.9 %) towards NEMS. The most participants were aware of the implementation time of NEMS (89.8 %) and zero mark-up policy (85.5 %) while the least learned of the adjustment time of National Essential Medicines List (NEML). Pharmacists from public secondary/tertiary hospitals tended to know more and have more positive attitudes. There was no statistical correlation between knowledge and attitude scores. The education level (p = 0.022) and number of training sessions attended (p = 0.028) were the only demographic variables linked to knowledge scores., Conclusions: Hospital pharmacists in Shaanxi Province had moderate knowledge of and attitudes towards the implementation of NEMS. Pharmacists from public secondary/tertiary hospitals showed better understanding. The government should therefore focus on improving the understanding of pharmacists in primary healthcare institutions and also address existing problems, especially the supply and distribution systems.
- Published
- 2016
- Full Text
- View/download PDF
28. Comparative studies of macrophage-biased responses in mice to infection with Toxoplasma gondii ToxoDB #9 strains of different virulence isolated from China.
- Author
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Zhang AM, Shen Q, Li M, Xu XC, Chen H, Cai YH, Luo QL, Chu DY, Yu L, Du J, Lun ZR, Wang Y, Sha Q, and Shen JL
- Subjects
- Animals, Blotting, Western, Cells, Cultured, China, Cytokines metabolism, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Gene Expression Profiling, Immunophenotyping, Mice, Mice, Inbred BALB C, Real-Time Polymerase Chain Reaction, Signal Transduction, Toxoplasma genetics, Toxoplasma isolation & purification, Virulence, Macrophage Activation, Macrophages, Peritoneal immunology, Toxoplasma immunology, Toxoplasma pathogenicity, Toxoplasmosis, Animal immunology
- Abstract
Background: Different from three clonal lineages of Toxoplasma gondii in North America and Europe, the genotype China 1 is predominantly prevalent in China. However, there are different virulent isolates within China 1, such as virulent TgCtwh3 and avirulent TgCtwh6, and little is known about differences in macrophage activation between them. The objective of this study focused on cytokine production, phenotype and markers of activated macrophages, and correlated signaling pathway induced by the two isolates., Methods: Adherent peritoneal macrophages (termed Wh3-Mφ and Wh6-Mφ, respectively) harvested from infected mice were cultured for detection of Nitric Oxide and arginase activity, and activated markers on Wh3-Mφ/Wh6-Mφ were determined by flow cytometry. In in vitro experiments, the levels of IL-12p40 and TNF-α were measured using ELISA kits, and mRNA expressions of IL-12p40, TNF-α, iNOS, Arg-1 and Ym1 were assayed by real-time PCR. To confirm the activation state of NF-kB p65 in infected cells stained by IF, protein levels of iNOS, Arg-1, Ym1, nuclear NF-κB p65, and phosphorylation of STAT6/STAT3/IκBα were evaluated by Western Blotting. A one-way ANOVA test was used to compare differences among multiple groups., Results: The result revealed that contrary to the virulent TgCtwh3, the less virulent TgCtwh6 isolate induced a significant increase in IL-12p40 and TNF-α. Although both isolates down-regulated CD80, CD86 and MHCII molecule expression on macrophages, TgCtwh3 promoted up-regulation of PD-L2 and CD206. Wh6-Mφ generated a high level of NO whereas Wh3-Mφ up-regulated Ym1 and arginase expression at transcriptional and protein levels. In terms of signaling pathway, TgCtwh3 induced phospho-STAT6, conversely, TgCtWh6 led to NF-κB p65 activation., Conclusions: The virulent TgCtwh3 isolate induced macrophages to polarize toward alternatively activated cells with STAT6 phosphorylation, whereas the less virulent TgCtwh6 elicited the development of classically activated macrophages with nuclear translocation of NF-κB p65. This discrepancy suggests that it is necessary to thoroughly analyze the genotype of TgCtwh3 and TgCtwh6, and to further study other effector molecules that contribute to the macrophage polarization in T. gondii.
- Published
- 2013
- Full Text
- View/download PDF
29. A case of bilateral pleural effusion as the first sign of multiple myeloma.
- Author
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Xu XL, Shen YH, Shen Q, and Zhou JY
- Subjects
- Biopsy, Bone Marrow pathology, Humans, Male, Middle Aged, Multiple Myeloma diagnostic imaging, Pleural Effusion diagnostic imaging, Pleural Effusion pathology, Radiography, Thoracic, Tomography, X-Ray Computed, Multiple Myeloma diagnosis, Multiple Myeloma etiology, Pleural Effusion complications
- Abstract
Multiple myeloma (MM) is a type of hematological malignancy that can affect all types of tissues in human. However, it is extremely rare that pleural effusion presents as the first sign in MM patients. A 54-year-old male patient attended our department of respiratory medicine complaining of shortness of breath for the past 3 months. A chest computer tomography (CT) radiograph revealed a bilateral pleural effusion, which was further assessed as exudative type. Sinus spiral CT scan demonstrated diffuse bone destruction of craniofacial bone. A broad reduction of the lumbar bone signal was confirmed by MRI. Furthermore, pleural biopsy showed abnormal proliferation of plasmocytes whereas bone marrow biopsy showed active hyperplasia of plasmacytoid cells. Interestingly, Bence-Jones protein in urine and serum protein electrophoresis was negative. The patient was diagnosed as non-secretory MM. He then underwent chemotherapy with vincristine, adriamycin and dexamethasone. Partial regression of the pleural effusion was achieved after two rounds of chemotherapy, and the patient has been followed for more than one year.
- Published
- 2013
- Full Text
- View/download PDF
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