1. Application of the amplification-free SERS-based CRISPR/Cas12a platform in the identification of SARS-CoV-2 from clinical samples.
- Author
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Liang, Jiajie, Teng, Peijun, Xiao, Wei, He, Guanbo, Song, Qifang, Zhang, Ying, Peng, Bin, Li, Gan, Hu, Liangshan, Cao, Donglin, and Tang, Yong
- Subjects
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SARS-CoV-2 , *REVERSE transcriptase polymerase chain reaction , *NUCLEIC acids , *RAMAN effect , *SERS spectroscopy - Abstract
The control of contagious or refractory diseases requires early, rapid diagnostic assays that are simple, fast, and easy-to-use. Here, easy-to-implement CRISPR/Cas12a-based diagnostic platform through Raman transducer generated by Raman enhancement effect, term as SERS-CRISPR (S-CRISPR), are described. The S-CRISPR uses high-activity noble metallic nanoscopic materials to increase the sensitivity in the detection of nucleic acids, without amplification. This amplification-free platform, which can be performed within 30–40 min of incubation time, is then used for detection of SARS-CoV-2 derived nucleic acids in RNA extracts obtained from nasopharyngeal swab specimens (n = 112). Compared with the quantitative reverse transcription polymerase chain reaction (RT-qPCR), the sensitivity and specificity of S-CRISPR reaches 87.50% and 100%, respectively. In general, the S-CRISPR can rapidly identify the RNA of SARS-CoV-2 RNA without amplification and is a potential strategy for nucleic acid point of care test (POCT). [ABSTRACT FROM AUTHOR]
- Published
- 2021
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