1. LMTK3 inhibition affects microtubule stability.
- Author
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Cilibrasi, Chiara, Ditsiou, Angeliki, Papakyriakou, Athanasios, Mavridis, George, Eravci, Murat, Stebbing, Justin, Gagliano, Teresa, and Giamas, Georgios
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TUBULINS ,CELL cycle proteins ,NUCLEAR proteins ,SCAFFOLD proteins ,CELL cycle regulation - Abstract
In addition, to confirm the involvement of NUSAP1 in the C28-mediated effect on microtubule stability and cell cycle arrest, we investigated the levels of cyclin-dependent kinase 1 (CDK1), a previously described NUSAP1-regulated protein [[12]], and phospho- III tubulin (S172). Taken together, although C28 can potentially bind tubulin, its relatively low binding affinity to purified tubulin dimer and the lack of any observable effect on tubulin polymerization suggest that C28 does not confer its effects by direct inhibition of tubulin polymerization. Considering the role of kinase inhibitors on microtubules [[4]], we investigated the possibility that C28 is a direct tubulin-targeting agent by employing an in vitro tubulin polymerization assay. Keywords: LMTK3; Kinase inhibitor; Breast cancer; Tubulin; NUSAP1 EN LMTK3 Kinase inhibitor Breast cancer Tubulin NUSAP1 1 6 6 04/12/21 20210409 NES 210409 The original online version of this article was revised: There are errors in Figures 2a and 2d. [Extracted from the article]
- Published
- 2021
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