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Background: Consumer preference is rapidly changing from 2D to 3D movies due to the sensational effects of 3D scenes, like those in Avatar and The Hobbit. Two 3D viewing technologies are available: active shutter glasses and passive polarized glasses. However, there are consistent reports of discomfort while viewing in 3D mode where the discomfort may refer to dizziness, headaches, nausea or simply not being able to see in 3D continuously. Methods: In this paper, we propose a theory that 3D technology which projects the two images (required for 3D perception) alternatively, cannot provide true 3D visual experience while the 3D technology projecting the two images simultaneously is closest to the human visual system for depth perception. Then we validate our theory by conducting experiments with 40 subjects and analyzing the EEG results of viewing 3D movie clips with passive polarized glasses while the images are projected simultaneously compared to 2D viewing. In addition, subjective feedback of the subjects was also collected and analyzed. Results: A higher theta and alpha band absolute power is observed across various areas including the occipital lobe for 3D viewing. We also found that the complexity of the signal, e.g. variations in EEG samples over time, increases in 3D as compared to 2D. Various results conclude that working memory, as well as, attention is increased in 3D viewing because of the processing of more data in 3D as compared to 2D. From subjective feedback analysis, 75% of subjects felt comfortable with 3D passive polarized while 25% preferred 3D active shutter technology. Conclusions: We conclude that 3D passive polarized technology provides more comfortable visualization than 3D active shutter technology. Overall, 3D viewing is more attractive than 2D due to stereopsis which may cause of high attention and involvement of working memory manipulations. [ABSTRACT FROM AUTHOR]

Background: There has been increasing interest recently in the plasticity of mesenchymal stem cells (MSCs) and their potential to differentiate into neural lineages. To unravel the roles and effects of different growth factors in the differentiation of MSCs into neural lineages, we have differentiated MSCs into neural lineages using different combinations of growth factors. Based on previous studies of the roles of insulin-like growth factor 1 (IGF-1) in neural stem cell isolation in the laboratory, we hypothesized that IGF-1 can enhance proliferation and reduce apoptosis in neural progenitor-like cells (NPCs) during differentiation of MSCs into NCPs. We induced MSCs differentiation under four different combinations of growth factors: (A) EGF + bFGF, (B) EGF + bFGF + IGF-1, (C) EGF + bFGF + LIF, (D) EGF + bFGF + BDNF, and (E) without growth factors, as a negative control. The neurospheres formed were characterized by immunofluorescence staining against nestin, and the expression was measured by flow cytometry. Cell proliferation and apoptosis were also studied by MTS and Annexin V assay, respectively, at three different time intervals (24 hr, 3 days, and 5 days). The neurospheres formed in the four groups were then terminally differentiated into neuron and glial cells. Results: The four derived NPCs showed a significantly higher expression of nestin than was shown by the negative control. Among the groups treated with growth factors, NPCs treated with IGF-1 showed the highest expression of nestin. Furthermore, NPCs derived using IGF-1 exhibited the highest cell proliferation and cell survival among the treated groups. The NPCs derived from IGF-1 treatment also resulted in a better yield after the terminal differentiation into neurons and glial cells than that of the other treated groups. Conclusions: Our results suggested that IGF-1 has a crucial role in the differentiation of MSCs into neuronal lineage by enhancing the proliferation and reducing the apoptosis in the NPCs. This information will be beneficial in the long run for improving both cell-based and cell-free therapy for neurodegenerative diseases. [ABSTRACT FROM AUTHOR]