1. Human pregnane X receptor is expressed in breast carcinomas, potential heterodimers formation between hPXR and RXR-alpha
- Author
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Isabel Conde, Julio Pérez, Benito Fraile, Ricardo Paniagua, Javier Zamora, Maria V.T. Lobo, Emilio Alba, Francisco González, María I. Arenas, Universidad de Alcalá. Departamento de Biología Celular y Genética, [Conde,I, Lobo,MVT, Pérez,J, Fraile,B, Paniagua,R, Arenas,MI] Department of Cell Biology and Genetics, University of Alcalá, Alcalá de Henares, Madrid, Spain. [Zamora,J] Clinical Biostatistics Unit, Hospital Ramón y Cajal, CIBER Epidemiología y Salud Pública (CIBERESP), Madrid, Spain. [González Sánchez,FJ, and Alba Conejo,E] Department of Medical Oncology, Hospital Universitario Virgen de la Victoria, Málaga, Spain. [Conde,I] Department of Cellular and Molecular Physiopathology, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
- Subjects
Ciencia ,SXR ,Pathology ,Receptors, Steroid ,Cancer Research ,Biología ,Named Groups::Persons::Age Groups::Adult::Aged::Aged, 80 and over [Medical Subject Headings] ,Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings] ,Supervivencia sin Enfermedad ,Receptores de Esteroides ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] ,pregnane X receptor ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Clinical Laboratory Techniques::Cytological Techniques::Histocytochemistry::Immunohistochemistry [Medical Subject Headings] ,Breast cancer ,CIENCIA ,Aged, 80 and over ,Pregnane X receptor ,Adulto ,Neoplasia de la mama ,Carcinoma, Ductal, Breast ,Femenino ,Pregnane X Receptor ,SCIENCE ,Middle Aged ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Receptors, Cytoplasmic and Nuclear::Receptors, Steroid [Medical Subject Headings] ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Glandular and Epithelial::Carcinoma::Adenocarcinoma::Carcinoma, Ductal::Carcinoma, Ductal, Breast [Medical Subject Headings] ,Immunohistochemistry ,Humanos ,Heterodimers ,Human pregnane X receptor ,Oncology ,Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Biochemical Processes::Dimerization [Medical Subject Headings] ,Female ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis::Neoplasm Staging [Medical Subject Headings] ,Dimerization ,Carcinoma in Situ ,Research Article ,Adult ,medicine.medical_specialty ,Western Blotting ,Science ,Anciano ,Receptores x Retinoide ,Blotting, Western ,Breast Neoplasms ,Retinoid X receptor ,Steroid biosynthesis ,Biology ,Carcinoma in situ ,lcsh:RC254-282 ,Disease-Free Survival ,medicine ,Named Groups::Persons::Age Groups::Adult [Medical Subject Headings] ,Genetics ,Dimerización ,Humans ,Named Groups::Persons::Age Groups::Adult::Aged [Medical Subject Headings] ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Prognosis::Disease-Free Survival [Medical Subject Headings] ,Diseases::Neoplasms::Neoplasms by Site::Breast Neoplasms [Medical Subject Headings] ,Aged ,Neoplasm Staging ,Receptor alfa X Retinoide ,Mediana edad ,Retinoid X Receptor alpha ,Retinoid X receptor alpha ,hPXR ,medicine.disease ,Diseases::Neoplasms::Neoplasms by Histologic Type::Neoplasms, Glandular and Epithelial::Carcinoma::Carcinoma in Situ [Medical Subject Headings] ,Genética ,Estadificación de Neoplasias ,Anciano de 80 o más Años ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Receptors, Cytoplasmic and Nuclear::Receptors, Retinoic Acid::Retinoid X Receptors [Medical Subject Headings] ,Retinoid X Receptors ,Nuclear receptor ,Check Tags::Female [Medical Subject Headings] ,Cancer cell ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques, Analytical::Electrophoresis::Blotting, Western [Medical Subject Headings] ,Cancer research ,Carcinoma ductal de mama ,Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Receptors, Cytoplasmic and Nuclear::Receptors, Retinoic Acid::Retinoid X Receptors::Retinoid X Receptor alpha [Medical Subject Headings] ,Inmunohistoquímica - Abstract
14 p.-5 fig.-5 tab., [Background] The human pregnane X receptor (hPXR) is an orphan nuclear receptor that induces transcription of response elements present in steroid-inducible cytochrome P-450 gene promoters. This activation requires the participation of retinoid X receptors (RXRs), needed partners of hPXR to form heterodimers. We have investigated the expression of hPXR and RXRs in normal, premalignant, and malignant breast tissues, in order to determine whether their expression profile in localized infiltrative breast cancer is associated with an increased risk of recurrent disease., [Methods] Breast samples from 99 patients including benign breast diseases, in situ and infiltrative carcinomas were processed for immunohistochemistry and Western-blot analysis., [Results] Cancer cells from patients that developed recurrent disease showed a high cytoplasmic location of both hPXR isoforms. Only the infiltrative carcinomas that relapsed before 48 months showed nuclear location of hPXR isoform 2. This location was associated with the nuclear immunoexpression of RXR-alpha., [Conclusions] Breast cancer cells can express both variants 1 and 2 of hPXR. Infiltrative carcinomas that recurred showed a nuclear location of both hPXR and RXR-alpha; therefore, the overexpression and the subcellular location changes of hPXR could be considered as a potential new prognostic indicator.
- Published
- 2008
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