9 results on '"Cao, Pengfei"'
Search Results
2. Long noncoding RNAs involvement in Epstein-Barr virus infection and tumorigenesis
- Author
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Zhang, Jing, Li, Xiaohan, Hu, Jingjin, Cao, Pengfei, Yan, Qijia, Zhang, Siwei, Dang, Wei, and Lu, Jianhong
- Published
- 2020
- Full Text
- View/download PDF
3. Correction to: miR-18a reactivates the Epstein-Barr virus through defective DNA damage response and promotes genomic instability in EBV-associated lymphomas
- Author
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Cao, Pengfei, Zhang, Meili, Wang, Lujuan, Sai, Buqing, Tang, Jiuqi, Luo, Zhaohui, Shuai, Cijun, Zhang, Liyang, Li, Zheng, Wang, Yanjin, Li, Guiyuan, and Xiang, Juanjuan
- Published
- 2019
- Full Text
- View/download PDF
4. miR-18a reactivates the Epstein-Barr virus through defective DNA damage response and promotes genomic instability in EBV-associated lymphomas
- Author
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Cao, Pengfei, Zhang, Meili, Wang, Lujuan, Sai, Buqing, Tang, Jiuqi, Luo, Zhaohui, Shuai, Cijun, Zhang, Liyang, Li, Zheng, Wang, Yanjin, Li, Guiyuan, and Xiang, Juanjuan
- Published
- 2018
- Full Text
- View/download PDF
5. Fluorescence in situ hybridization is superior for monitoring Epstein Barr viral load in infectious mononucleosis patients.
- Author
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Pengfei Cao, Meili Zhang, Wei Wang, Yafei Dai, Buqing Sai, Jun Sun, Lujuan Wang, Fan Wang, Guiyuan Li, Juanjuan Xiang, Cao, Pengfei, Zhang, Meili, Wang, Wei, Dai, Yafei, Sai, Buqing, Sun, Jun, Wang, Lujuan, Wang, Fan, Li, Guiyuan, and Xiang, Juanjuan
- Subjects
EPSTEIN-Barr virus diseases ,EPSTEIN-Barr virus ,MONONUCLEOSIS ,ETIOLOGY of diseases ,FLUORESCENCE in situ hybridization ,POLYMERASE chain reaction ,DIAGNOSIS ,BLOOD plasma ,DNA ,PROTEINS ,HEMOPHAGOCYTIC lymphohistiocytosis ,VIRAL antigens ,VIRAL load ,CASE-control method ,MONONUCLEAR leukocytes - Abstract
Background: Epstein Barr virus (EBV) plays a causal role in some diseases, including infectious mononucleosis, lymphoproliferative diseases and nasopharyngeal carcinoma. Detection of EBV infection has been shown to be a useful tool for diagnosing EBV-related diseases. In the present study, we compared the performance of molecular tests, including fluorescence in situ hybridization (FISH) and EBV real-time PCR, to those of serological assays for the detection of EBV infection.Methods: Thirty-eight patients with infectious mononucleosis (IM) were enrolled, of whom 31 were diagnosed with a mild type, and seven were diagnosed with IM with haemophagocytic lymphohistiocytosis and chronic active EBV infection. Twenty healthy controls were involved in the study. The atypical lymphocytes in peripheral blood were detected under a microscope and the percentage of positive cells was calculated. EBV DNA load in peripheral blood was detected using real-time PCR. The FISH assay was developed to detect the EBV genome from peripheral blood mononuclear cells (PBMC). Other diagnosis methods including the heterophil agglutination (HA) test and EBV-VCA-IgM test, to detect EBV were also compared. SPSS17.0 was used for statistical analysis.Results: In all, 5-41% atypical lymphocytes were found among the PBMC in mild IM patients, whereas 8-51% atypical lymphocytes were found in IM patients with haemophagocytic lymphohistiocytosis and chronic active EBV infection patients. There was no significant difference in the ratios of atypical lymphoma between patients of the different types. We observed that 71.2% of mild IM patients and 85.7% of IM patients with haemophagocytic lymphohistiocytosis and chronic active EBV infection patients were positive for EBV-VCA-IgM. EBV-VCA-IgM was negative in all healthy control subjects. In addition, 67.1% of mild IM patients tested heterophile antibody positive, whereas 71.4% of IM patients with haemophagocytic lymphohistiocytosis and chronic active EBV infection tested positive. EBV DNA detected using real-time PCR was observed in 89.5% of these IM patients. The EBV genome was detected by the FISH assay in 97.4% of the IM patients. The EB viral loads detected by FISH and real-time PCR increased with the severity of IM. The EBV genome was detected in almost all the PBMC of IM with haemophagocytic lymphohistiocytosis and chronic active EBV infection patients.Conclusion: Molecular tests, including FISH and EBV real-time PCR, are more sensitive than serological assays for the detection of EBV infection. The FISH assay detecting EBV copies in unfractionated whole blood is preferable and superior to plasma real-time PCR in its reflection of the absolute viral burden circulating in the patients. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
6. Prognostic value of the distance between the primary tumor and brainstem in the patients with locally advanced nasopharyngeal carcinoma.
- Author
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Yuxiang He, Ying Wang, Lin Shen, Yajie Zhao, Pengfei Cao, Mingjun Lei, Dengming Chen, Tubao Yang, Liangfang Shen, Shousong Cao, He, Yuxiang, Wang, Ying, Shen, Lin, Zhao, Yajie, Cao, Pengfei, Lei, Mingjun, Chen, Dengming, Yang, Tubao, Shen, Liangfang, and Cao, Shousong
- Subjects
NASOPHARYNX cancer ,BRAIN stem ,INTENSITY modulated radiotherapy ,CANCER relapse ,RETROSPECTIVE studies ,COMPARATIVE studies ,RESEARCH methodology ,MEDICAL cooperation ,NASOPHARYNX tumors ,PROGNOSIS ,RADIOTHERAPY ,RESEARCH ,EVALUATION research ,RELATIVE medical risk ,PREDICTIVE tests ,RECEIVER operating characteristic curves ,DIAGNOSIS - Abstract
Background: Brainstem dose limitations influence radiation dose reaching to tumor in the patients with locally-advanced nasopharyngeal cancer (NPC).Methods: A retrospective analysis of the prognostic value of the distance between the primary tumor and brainstem (Dbs) in 358 patients with locally-advanced NPC after intensity-modulated radiation therapy (IMRT). Receiver operating characteristic (ROC) curves were used to identify the cut-off value to analyze the impact of Dbs on tumor dose coverage and prognosis.Results: The three-year overall survival (OS), local relapse-free survival (LRFS), distant metastasis-free survival (DMFS), and disease-free survival (DFS) were 88.8 vs. 78.4% (P = 0.007), 96.5 vs. 91.1% (P = 0.018), 87.8 vs. 79.3% (P = 0.067), and 84.1 vs. 69.6% (P = 0.002) for the patients with the Dbs > 4.7 vs. ≤ 4.7 mm, respectively. ROC curves revealed Dbs (4.7 mm) combined with American Joint Committee on Cancer (AJCC) T classification had a significantly better prognostic value for OS (P < 0.05).Conclusions: Dbs (≤ 4.7 mm) is an independent negative prognostic factor for OS/LRFS/DFS and enhances the prognostic value of T classification in the patients with locally-advanced NPC. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
7. Fluorescence in situ hybridization is superior for monitoring Epstein Barr viral load in infectious mononucleosis patients.
- Author
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Cao P, Zhang M, Wang W, Dai Y, Sai B, Sun J, Wang L, Wang F, Li G, and Xiang J
- Subjects
- Adolescent, Antigens, Viral blood, Capsid Proteins blood, Case-Control Studies, Child, Child, Preschool, DNA, Viral blood, Epstein-Barr Virus Infections diagnosis, Epstein-Barr Virus Infections virology, Female, Herpesvirus 4, Human immunology, Herpesvirus 4, Human pathogenicity, Humans, Infant, Leukocytes, Mononuclear virology, Lymphohistiocytosis, Hemophagocytic virology, Male, Plasma virology, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Herpesvirus 4, Human genetics, In Situ Hybridization, Fluorescence methods, Infectious Mononucleosis virology, Viral Load methods
- Abstract
Background: Epstein Barr virus (EBV) plays a causal role in some diseases, including infectious mononucleosis, lymphoproliferative diseases and nasopharyngeal carcinoma. Detection of EBV infection has been shown to be a useful tool for diagnosing EBV-related diseases. In the present study, we compared the performance of molecular tests, including fluorescence in situ hybridization (FISH) and EBV real-time PCR, to those of serological assays for the detection of EBV infection., Methods: Thirty-eight patients with infectious mononucleosis (IM) were enrolled, of whom 31 were diagnosed with a mild type, and seven were diagnosed with IM with haemophagocytic lymphohistiocytosis and chronic active EBV infection. Twenty healthy controls were involved in the study. The atypical lymphocytes in peripheral blood were detected under a microscope and the percentage of positive cells was calculated. EBV DNA load in peripheral blood was detected using real-time PCR. The FISH assay was developed to detect the EBV genome from peripheral blood mononuclear cells (PBMC). Other diagnosis methods including the heterophil agglutination (HA) test and EBV-VCA-IgM test, to detect EBV were also compared. SPSS17.0 was used for statistical analysis., Results: In all, 5-41% atypical lymphocytes were found among the PBMC in mild IM patients, whereas 8-51% atypical lymphocytes were found in IM patients with haemophagocytic lymphohistiocytosis and chronic active EBV infection patients. There was no significant difference in the ratios of atypical lymphoma between patients of the different types. We observed that 71.2% of mild IM patients and 85.7% of IM patients with haemophagocytic lymphohistiocytosis and chronic active EBV infection patients were positive for EBV-VCA-IgM. EBV-VCA-IgM was negative in all healthy control subjects. In addition, 67.1% of mild IM patients tested heterophile antibody positive, whereas 71.4% of IM patients with haemophagocytic lymphohistiocytosis and chronic active EBV infection tested positive. EBV DNA detected using real-time PCR was observed in 89.5% of these IM patients. The EBV genome was detected by the FISH assay in 97.4% of the IM patients. The EB viral loads detected by FISH and real-time PCR increased with the severity of IM. The EBV genome was detected in almost all the PBMC of IM with haemophagocytic lymphohistiocytosis and chronic active EBV infection patients., Conclusion: Molecular tests, including FISH and EBV real-time PCR, are more sensitive than serological assays for the detection of EBV infection. The FISH assay detecting EBV copies in unfractionated whole blood is preferable and superior to plasma real-time PCR in its reflection of the absolute viral burden circulating in the patients.
- Published
- 2017
- Full Text
- View/download PDF
8. Prognostic value of the distance between the primary tumor and brainstem in the patients with locally advanced nasopharyngeal carcinoma.
- Author
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He Y, Wang Y, Shen L, Zhao Y, Cao P, Lei M, Chen D, Yang T, Shen L, and Cao S
- Subjects
- Carcinoma, Female, Humans, Male, Middle Aged, Nasopharyngeal Carcinoma, Nasopharyngeal Neoplasms epidemiology, Nasopharyngeal Neoplasms radiotherapy, Predictive Value of Tests, Prognosis, ROC Curve, Radiotherapy, Intensity-Modulated, Retrospective Studies, Risk, Brain Stem pathology, Nasopharyngeal Neoplasms diagnosis, Nasopharyngeal Neoplasms pathology
- Abstract
Background: Brainstem dose limitations influence radiation dose reaching to tumor in the patients with locally-advanced nasopharyngeal cancer (NPC)., Methods: A retrospective analysis of the prognostic value of the distance between the primary tumor and brainstem (Dbs) in 358 patients with locally-advanced NPC after intensity-modulated radiation therapy (IMRT). Receiver operating characteristic (ROC) curves were used to identify the cut-off value to analyze the impact of Dbs on tumor dose coverage and prognosis., Results: The three-year overall survival (OS), local relapse-free survival (LRFS), distant metastasis-free survival (DMFS), and disease-free survival (DFS) were 88.8 vs. 78.4% (P = 0.007), 96.5 vs. 91.1% (P = 0.018), 87.8 vs. 79.3% (P = 0.067), and 84.1 vs. 69.6% (P = 0.002) for the patients with the Dbs > 4.7 vs. ≤ 4.7 mm, respectively. ROC curves revealed Dbs (4.7 mm) combined with American Joint Committee on Cancer (AJCC) T classification had a significantly better prognostic value for OS (P < 0.05)., Conclusions: Dbs (≤ 4.7 mm) is an independent negative prognostic factor for OS/LRFS/DFS and enhances the prognostic value of T classification in the patients with locally-advanced NPC.
- Published
- 2016
- Full Text
- View/download PDF
9. HIF-1A and C/EBPs transcriptionally regulate adipogenic differentiation of bone marrow-derived MSCs in hypoxia.
- Author
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Jiang C, Sun J, Dai Y, Cao P, Zhang L, Peng S, Zhou Y, Li G, Tang J, and Xiang J
- Subjects
- Adipocytes cytology, Adipocytes metabolism, Adipogenesis, Alkaline Phosphatase metabolism, Antigens, CD metabolism, CCAAT-Enhancer-Binding Protein-delta genetics, CCAAT-Enhancer-Binding Protein-delta metabolism, CCAAT-Enhancer-Binding Proteins genetics, Cells, Cultured, Chondrocytes cytology, Chondrocytes metabolism, Endoglin, Gene Expression Regulation, Humans, Hyaluronan Receptors metabolism, Hypoxia-Inducible Factor 1, alpha Subunit antagonists & inhibitors, Hypoxia-Inducible Factor 1, alpha Subunit genetics, Mesenchymal Stem Cells metabolism, Osteogenesis, RNA Interference, RNA, Small Interfering metabolism, Receptors, Cell Surface metabolism, Bone Marrow Cells cytology, CCAAT-Enhancer-Binding Proteins metabolism, Cell Hypoxia, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Mesenchymal Stem Cells cytology
- Abstract
Introduction: Bone marrow-derived mesenchymal stem cells (BMSCs, also known as bone marrow-derived mesenchymal stromal cells) are known to be a component of the tumor microenvironment. BMSCs are multipotent stromal cells that can differentiate into a variety of cell types, including osteocytes, chondrocytes, adipocytes, epithelial cells and endothelial cells. Stem cells found in niches or transplanted into injured tissues constantly encounter hypoxic stress. Areas with very low to no oxygen pressure exist in solid tumors. The differentiation capacity of BMSCs under hypoxic conditions remains controversial., Methods: In this study, a hypoxic workstation, set at an oxygen concentration of 0.2% was used to mimic the hypoxic microenvironment of cancer in vivo. Oil red O staining and alkaline phosphatase staining were used to examine the adipogenic or osteogenic differentiation, respectively, of BMSCs. Real-time PCR was performed to explore the expression of adipocyte- or osteocyte-specific genes. An RT2 Profiler™ PCR Array was used to screen a panel of 84 genes associated with human adipogenesis in BMSCs under normal and hypoxic conditions. A dual-luciferase reporter assay and chromatin immunoprecipitation (ChIP) were applied to analyze promoter activity to evaluate the possible regulatory mechanism of adipocyte-specific gene expression., Results: We found that this extreme hypoxia impaired osteogenic differentiation as indicated by the attenuation of alkaline phosphatase (ALP) activity and the reduced expression of osteogenic markers osteocalcin and osteopontin. Moreover, extreme hypoxia enhanced adipogenic differentiation, as indicated by the accumulation of lipid droplets and the expression of the adipocyte-specific genes leptin, LPL, CFD, PGAR and HIG2. In the extreme hypoxic conditions (0.2% oxygen), the overexpression of CCAAT enhancer-binding proteins (C/EBPs), especially C/EBPδ, and HIF-1A upregulated the promoter activities of adipocyte-specific genes such as leptin, CFD, HIG2, LPL, PGAR. In the present study, peroxisome proliferator-activated receptor-gamma (PPARγ) exerted a negative effect on the differentiation of BMSCs into adipocytes., Conclusions: In view of these findings, extreme hypoxia induced the adipogenic differentiation of BMSCs through HIF-1A and C/EBPs. These findings might provide clues regarding the roles of BMSCs in the cancer microenvironment.
- Published
- 2015
- Full Text
- View/download PDF
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