15 results on '"Chao Zhao"'
Search Results
2. Clinical practice guideline for transurethral plasmakinetic resection of prostate for benign prostatic hyperplasia (2021 Edition)
- Author
-
Zeng, Xian-Tao, Jin, Ying-Hui, Liu, Tong-Zu, Chen, Fang-Ming, Ding, De-Gang, Fu, Meng, Gu, Xin-Quan, Han, Bang-Min, Huang, Xing, Hou, Zhi, Hu, Wan-Li, Kang, Xin-Li, Li, Gong-Hui, Li, Jian-Xing, Li, Pei-Jun, Liang, Chao-Zhao, Liu, Xiu-Heng, Liu, Zhi-Yu, Liu, Chun-Xiao, Liu, Jiu-Min, Luo, Guang-Heng, Luo, Yi, Qin, Wei-Jun, Qiu, Jian-Hong, Qiu, Jian-Xin, Shang, Xue-Jun, Shi, Ben-Kang, Sun, Fa, Tian, Guo-Xiang, Tian, Ye, Wang, Feng, Wang, Feng, Wang, Yin-Huai, Wang, Yu-Jie, Wang, Zhi-Ping, Wang, Zhong, Wei, Qiang, Xiao, Min-Hui, Xu, Wan-Hai, Yi, Fa-Xian, Zhu, Chao-Yang, Zhuang, Qian-Yuan, Zhou, Li-Qun, Zou, Xiao-Feng, Xing, Nian-Zeng, He, Da-Lin, and Wang, Xing-Huan
- Published
- 2022
- Full Text
- View/download PDF
3. Genomic landscape and its correlations with tumor mutational burden, PD-L1 expression, and immune cells infiltration in Chinese lung squamous cell carcinoma
- Author
-
Jinpeng Shi, Xianchao Guo, Xuefei Li, Wei Zhu, Chunxia Su, Xiaoxia Chen, Likun Hou, Zhengwei Dong, Ji He, Henghui Zhang, Shengxiang Ren, Beibei Mao, Chao Zhao, Chunyan Wu, Tao Jiang, and Caicun Zhou
- Subjects
0301 basic medicine ,Nonsynonymous substitution ,Male ,Cancer Research ,Lung Neoplasms ,medicine.medical_treatment ,Lymphocyte ,CD8-Positive T-Lymphocytes ,B7-H1 Antigen ,0302 clinical medicine ,Tumor Microenvironment ,Hematology ,Genome ,Kelch-Like ECH-Associated Protein 1 ,biology ,Immune cells ,lcsh:Diseases of the blood and blood-forming organs ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Immunohistochemistry ,Female ,PD-L1 ,medicine.medical_specialty ,China ,Class I Phosphatidylinositol 3-Kinases ,lcsh:RC254-282 ,03 medical and health sciences ,Mutation Accumulation ,Immune system ,Lymphocytes, Tumor-Infiltrating ,Asian People ,Internal medicine ,Lung squamous cell carcinoma ,medicine ,Humans ,Molecular Biology ,Aged ,Retrospective Studies ,lcsh:RC633-647.5 ,TMB ,Research ,Immunotherapy ,030104 developmental biology ,Mutation ,Cancer research ,biology.protein ,CD8 - Abstract
Introduction To depict the genomic landscape of Chinese early-stage lung squamous cell carcinoma (LUSC) and investigate its correlation with tumor mutation burden (TMB), PD-L1 expression, and immune infiltrates. Methods Whole-exome sequencing was performed on 189 surgically resected LUSC. TMB was defined as the sum of nonsynonymous single nucleotide and indel variants. CD8+ tumor-infiltrating lymphocyte (TIL) density and PD-L1 expression were evaluated by immunohistochemistry. Six immune infiltrates were estimated using an online database. Results The median TMB was 9.43 mutations per megabase. Positive PD-L1 expression and CD8+ TILs density were identified in 24.3% and 78.8%. PIK3CA amplification was associated with significantly higher TMB (P = 0.036). Frequent genetic alterations had no impact on PD-L1 expression but PIK3CA amplification and KEAP1 mutation were independently associated with significantly lower CD8+ TIL density (P
- Published
- 2019
4. In vivo tracking on longer retention of transplanted myocardin gene-modified adipose-derived stem cells to improve erectile dysfunction in diabetic rats
- Author
-
Shan-chao Zhao, Shuhua He, Anyang Wei, Ze-Rong Chen, Zhiqiang Wang, Fengzhi Chen, Yan-Bing Liang, Wei Ding, Li Wang, and Haibo Zhang
- Subjects
0301 basic medicine ,Male ,medicine.medical_treatment ,Medicine (miscellaneous) ,Apoptosis ,Pharmacology ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,0302 clinical medicine ,Diabetes mellitus ,Erectile Dysfunction ,lcsh:QD415-436 ,lcsh:R5-920 ,medicine.diagnostic_test ,Penile Erection ,Nuclear Proteins ,Cell Differentiation ,Stem-cell therapy ,Erectile dysfunction (ED) ,Cell tracking ,030220 oncology & carcinogenesis ,Molecular Medicine ,Stem cell ,lcsh:Medicine (General) ,Mesenchymal Stem Cell Transplantation ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Flow cytometry ,Diabetes Mellitus, Experimental ,lcsh:Biochemistry ,03 medical and health sciences ,In vivo ,Adipose-derived stem cells (ASCs) ,medicine ,Oil Red O ,Animals ,Humans ,CD90 ,Cell Proliferation ,Cell growth ,business.industry ,Research ,Myocardin ,Mesenchymal Stem Cells ,Muscle, Smooth ,Cell Biology ,Rats ,Disease Models, Animal ,030104 developmental biology ,chemistry ,Trans-Activators ,business - Abstract
Background Stem cell therapy has revealed a promising future for treating erectile dysfunction (ED), but the fate and curative mechanism of intracavernosal transplanted stem cells are under further exploration. This study aimed to demonstrate the effects of myocardin gene modification on improving erectile function and prolonging the retention of implanted adipose-derived stem cells (ASCs) using in vivo small animal imaging. Methods ASCs were isolated, cultured, and identified by flow cytometry and osteogenic and adipogenic induction. The effects of gene modification on cell proliferation, apoptosis, and contraction were determined by CCK-8, EdU, flow cytometry, and collagen gel lattice contraction assays as well as confocal microscopy. A total of 20 normal and 60 diabetes mellitus ED to (DMED) Sprague–Dawley rats were recruited to the 7 day and 21 day groups. Each group contained subgroups of 10 rats each: the negative control (NC), DMED + ASCs plus Ad-Luc-Myocardin, DMED + ASCs plus Ad-Luc, and DMED + phosphate buffer solution (PBS) groups. Erectile function was evaluated with the intracavernosal pressure/mean arterial pressure (△ICP/MAP) ratio. In vivo small animal imaging and an EdU cell tracking strategy were introduced to detect the transplanted ASCs, and IHC and WB were performed to assess smooth muscle cell protein levels. Results The ASCs expressed high CD29 and CD90 and scant CD45, while the multi-induction potential was verified by oil red O and alizarin red staining. Gene transfection of myocardin had no significant influence on ASC apoptosis but inhibited cell proliferation and promoted cell contraction. Myocardin combined with ASCs enhanced the therapeutic potential of ASCs for improving the △ICP/MAP ratio as well as α-SMA and calponin expression. In vivo imaging confirmed that ASCs resided within the cavernous body in 21 days, while only a few red EdU dots were detected. Conclusions Myocardin induced ASC differentiation towards smooth muscle-like cells and enhanced the therapeutic potential of ASCs for ameliorating ED in STZ-induced diabetic rats. Notably, in vivo small animal tracking was an effective strategy for monitoring the implanted stem cells, and this strategy might have advantages over traditional EdU assays. Electronic supplementary material The online version of this article (10.1186/s13287-019-1325-7) contains supplementary material, which is available to authorized users.
- Published
- 2019
5. Label free quantitative proteomics reveals the role of miR-200b in androgen-independent prostate cancer cells.
- Author
-
Minyi He, Mengzhuang Gou, Min Qi, Wei Xiang, Zhicheng Ji, Wen-Jie Wang, Shan-Chao Zhao, and Yawei Liu
- Subjects
PROSTATE cancer ,PROTEOMICS ,MICRORNA ,ANDROGENS ,CANCER cells - Abstract
Background: Our previous studies indicated that miR-200b inhibits the growth of androgen-independent prostate cancer (AIPC) cells. In this study, we employed quantitative proteomics techniques to unravel the role of miR-200b in AIPC. Methods: miR-200b was over-expressed or inhibited by transfection with miR-200b mimics or miR-200b inhibitor in PC3 cells. Total proteins were collected and the profiles of different groups were analyzed by label-free proteomics. PANTHER was applied to analyze biological processes, molecular functions and pathways of proteins regulated by miR-200b. miRBase was used to evaluate target genes of miR-200b in proteins regulated by miR-200b. Results: Thirteen proteins were up-regulated in miR-200b mimics/mimics NC (negative miRNA control) and 14 proteins were down-regulated; 67 proteins were up-regulated in miR-200b inhibitor/inhibitor NC and 98 proteins were down-regulated. There were seven proteins which were both down-regulated by miR-200b mimics and up-regulated by miR-200b inhibitor, TM4SF1, YAP1, PPP1R2, MARCKS, RTN4, GLIPR2 and SUCLG1. Among these, TM4SF1, YAP1, PPP1R2, MARCKS, RTN4 were predicted as target genes of miR-200b by miRBase, while GLIPR2 and SUCLG1 were not. Conclusion: This work identified several target genes of miR-200b by label free proteomics method, i.e., TM4SF1, YAP1, PPP1R2, MARCKS, RTN4, GLIPR2, and SUCLG1. The signaling pathways regulated by these proteins such as Hippo signaling may contribute to the phenotype resulting from miR-200b. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
6. Characterization of the peripheral blood transcriptome and adaptive evolution of the MHC I and TLR gene families in the wolf (Canis lupus).
- Author
-
Guangshuai Liu, Honghai Zhang, Guolei Sun, Chao Zhao, Shuai Shang, Xiaodong Gao, Tian Xia, and Xiufeng Yang
- Subjects
WOLF behavior ,MAMMAL adaptation ,RNA sequencing ,MAJOR histocompatibility complex genetics ,TOLL-like receptors - Abstract
Background: The wolf (Canis lupus) is one of the most widely distributed terrestrial mammals, because it is well adapted to various ecological niches and their corresponding pathogen environments. Immunological competence is a crucial factor involved in adapting to a changing environment and fighting pathogen infection in animals. In this study, the peripheral blood transcriptome of wolves was generated via RNA-seq to advance understanding of the wolf immunome, with a special focus on the major histocompatibility complex class I (MHC I) and toll-like receptor (TLR) gene families, which are involved in pathogen recognition and defense. Results: The blood transcriptomic libraries of eight wolves originating from Tibet and Inner Mongolia were sequenced, and approximately 383 million reads were generated. Using a genome-guided assembly strategy, we obtained 123,851 unigenes, with a mean length of 845 bp and an N50 length of 1121 bp. On the basis of BLAST searches against the NCBI non-redundant protein database (Nr), a total of 36,192 (29.22%) unigenes were annotated. For functional classification, 24,663 unigenes were assigned to 13,016 Gene Ontology (GO) terms belonging to 51 sub-categories of the three main GO categories. Additionally, 7682 unigenes were classified into 6 Kyoto Encyclopedia of Genes and Genomes (KEGG) categories, in which the most represented functional sub-categories were signal transduction and the immune system, and 16,238 unigenes were functionally classified into 25 Eukaryotic Orthologous Groups (KOG) categories. We observed an overall higher ω (d
N /dS ) value at antigen-binding sites (ABSs) than at non-ABS regions as well as clear evidence of intergenic/intragenic recombination events at wolf MHC I loci. Additionally, our analysis revealed that carnivorous TLRs were dominated by purifying selection, with mean ω values at each TLR locus ranging from 0.173 to 0.527. However, we also found significant instances of positive selection that acted on several codons in pathogen recognition domains and were linked to species-specific differences in pathogen recognition. Conclusions: This study represents the first attempt to characterize the blood transcriptome of the wolf and to highlight the value of investigating the immune system. Balancing selection and recombination have contributed to the historical evolution of wolf MHC I genes. Moreover, TLRs in carnivores have undergone adaptive evolution against the background of purifying selection, and a high level of adaptive evolution was detected in the wolf TLR system. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
7. Genotoxicity and acute and subchronic toxicity studies of a bioactive polyoxometalate in Wistar rats.
- Author
-
Xiaofeng Qu, Kun Xu, Chao Zhao, Xiuling Song, Jinhua Li, Li Li, Wei Nie, Hao Bao, Juan Wang, Fenglan Niu, and Juan Li
- Subjects
ANTIVIRAL agents ,DRUG toxicity ,CELL lines ,CELL culture ,GENETIC toxicology - Abstract
Background: Cs
2 K4 Na [SiW9 Nb3 O40 ] (POM93) is a novel broad-spectrum antiviral agent with high activity, high stability, and low toxicity in vitro. Most toxicity studies for POM93 have been performed in cultured cell lines rather than in animals. Like other POMs, there is a lack of evidence for in vivo toxicity limits, oral bioavailability, and therapeutic applications. Methods: The toxic properties of POM93 were evaluated comprehensively in vivo, including the acute and subchronic oral toxicity studies and genotoxicity tests. Results: The acute toxicity study showed no abnormal changes or mortality in rats treated with POM93 even at the single high dose of 5000 mg/kg body weight. In the subchronic toxicity study, regardless of the body weight, the organ weight, and the hematological parameters, similar results were observed between the control group and the experimental groups. POM93 produced mild changes in rare hematological parameters in the liver and kidneys, but did not induce the clinical symptoms of liver or kidneys injury in rats as confirmed by histopathological analysis. Moreover, neither mutagenicity nor clastogenicity was caused by POM93 treatment in vitro and in vivo. Conclusions: The present study demonstrates that the oral administration of POM93 is presumed safe and poses a low risk of potential health risks. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
8. Epidemiological study of HER-2 mutations among EGFR wild-type lung adenocarcinoma patients in China.
- Author
-
Xuefei Li, Chao Zhao, Chunxia Su, Shengxiang Ren, Xiaoxia Chen, and Caicun Zhou
- Subjects
- *
LUNG cancer & genetics , *GENETIC mutation , *EPIDERMAL growth factor receptors , *LUNG cancer patients , *ADENOCARCINOMA , *PROTEIN overexpression , *IMMUNOHISTOCHEMISTRY - Abstract
Background: Human epidermal growth factor receptor (HER)-2 is a driver gene in non-small cell lung cancer (NSCLC). The present study evaluated the mutation rate of HER-2 within the wild-type epidermal growth factor receptor (EGFR) lung adenocarcinoma population in China. Methods: Formalin-fixed, paraffin-embedded samples from 456 patients with wild-type EGFR lung adenocarcinoma were analyzed for HER-2 mutations by amplification-refractory mutation system (ARMS), and HER-2 protein expression was evaluated by immunohistochemistry. All samples positive for HER-2 mutation underwent direct sequencing for further verification. Results: HER-2 mutation was detected in 22/456 cases (4.8 %); the rate was 6.7 % among 331 triple-negative samples (i.e., wild-type EGFR, anaplastic lymphoma kinase, and ROS proto-oncogene 1). Direct sequencing confirmed that the results were consistent with those obtained by ARMS analysis in 19 cases. The positive rate was 15.4 % by immunohistochemical analysis of HER-2 expression; this was not correlated with mutation rate. HER-2 mutation and positivity were not correlated with gender, age, smoking status, disease stage, or histological subtype. The 22 cases of HER-2 mutations occurred only in acinar (36.4 %), papillary (36.4 %), minimally invasive (13.6 %), solid (9.2 %), and invasive mucinous (4.5 %) subtypes. Disease-free and overall survival were not associated with HER-2 mutation or HER-2 protein overexpression. Conclusion: The HER-2 mutation rate was 4.8 % among EGFR wild-type lung adenocarcinoma patients in China, and 6.7 % among driver genes, triple-negative lung adenocarcinoma. The incidence of HER-2 mutation varied among different lung adenocarcinoma subtypes, occurring mainly in acinar and papillary predominant subtypes. 15.4 % of EGFR wild-type lung adenocarcinoma patients showed HER-2 protein overexpression, but this was not correlated to HER-2 mutation. Existing follow-up data did not show a correlation between HER-2 mutation with DFS or OS. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
9. Comparison of the sagittal profiles among thoracic idiopathic scoliosis patients with different Cobb angles and growth potentials.
- Author
-
Bo Ran, Guo-you Zhang, Feng Shen, Jia-yu Chen, Ji-bin Wu, Feng-chao Zhao, Kai-jin Guo, Dun-yi Qi, Bing Zhou, Xiang-yang Chen, Xin-zhu Zhang, Yue-hua Qiao, and Ming Li
- Abstract
Background: Previous studies have demonstrated that pelvic incidence and sacral slope are significantly greater in idiopathic scoliosis patients compared with normal adolescents. However, whether these sagittal parameters are related to the progression of scoliosis remain unknown. The present was designed to determine the differences in the sagittal profiles among thoracic idiopathic scoliosis patients with different potentials for curve progression. Methods: Ninety-seven outpatient idiopathic scoliosis patients enrolled from June 2008 to June 2011 were divided to three groups according to different Cobb angles and growth potentials: (1) non-progression of thoracic curve group, Risser sign of 5 and Cobb’s angle < 40°; (2) moderate progression of thoracic curve group, Risser sign of 5 and Cobb’s angle ≥ 40°; and (3) severe progression of thoracic curve group, Risser sign ≤ 3 and Cobb’s angle ≥ 40°. All patients underwent whole spinal anteroposterior and lateral X-ray in standing position, and the sagittal parameters were measured, including thoracic kyphosis, lumbar lordosis, sacral slope, pelvic incidence, and pelvic tilt. Results: The average thoracic scoliosis Cobb’s angle in the non-progression group was significantly less than that in the moderate progression group (P < 0.01) and severe progression group (P < 0.01), but there was no statistical difference in the average thoracic scoliosis Cobb’s angle between the severe progression group and moderate progression group. The average thoracic kyphosis angle in the severe progression group (9° ± 4°) was significantly smaller than that in the non-progression group (18° ± 6°, P < 0.01) and moderate progression group (14° ± 5°, P < 0.05). No statistical differences were present in the average lumbar lordosis, sacral slope, pelvic incidence, and pelvic tilt among the three groups. Conclusions: Thoracic hypokyphosis is strongly related with the curve progression in thoracic idiopathic scoliosis patients, but not pelvic sagittal profiles. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
10. Sorting and identification of side population cells in the human cervical cancer cell line HeLa.
- Author
-
Wenjuan Qi, Chao Zhao, Lijun Zhao, Ning Liu, Xiaoping Li, Weidong Yu, and Lihui Wei
- Subjects
- *
CERVICAL cancer , *STEM cells , *CANCER cells , *CELL lines , *TRICHOSTATIN A - Abstract
Background Several reports have revealed that cancer stem cells (CSCs) exist in many types of solid tumors. Some studies have demonstrated that side population (SP) cells isolated from diverse cancer lines harbor cancer stem-like properties, but there are few reports examining the characteristic of SP cells in human cervical cancer. The aim of this study is 1)to find out a feasible way to detect the tumor stem-like cells in cervical cancer, and 2)to analyze the properties of the SP cells being sorted. Methods Isolated SP and non-SP cells from human cervical cancer cell line Hela by Hoechst33342 dying method and flow cytometry analysis. Observing morphology of SP and non-SP cells. The expression of various biomarkers putatively related to cancer stem cells were investigated by immucytochemistry of SP and non-SP cells. We also analyzed cell cycle and cell apoptosis for sorted cells. The oncogenicity of the SP and non-SP cells were analyzed by tumor formation in nonobesediabeti- c/severe combined immune- deficient (NOD/SCID) mice. The drug-resistant and radiation-resistant index between SP, non-SP and Hela cells was estimated by MTS assay. Results The fraction of SP cells in Hela was approximately 1.07 ± 0.32%. SP cells were smaller and rounder in shape than non-SP cells, and mostly showed colony-like growth. Immunocytochemistry showed that stem cell makers (Oct3/4, CD133, BCRP) were highly expressed in SP cells. Moreover, the number of apoptotic cells among non-SP cells (17.6 ± 3.7%) was significantly higher compared with that among SP cells (4.4 ± 1.2%). The HE staining of in vivo grown tumors result from SP cells showed more poor differentiation, though no significant differences were shown between SP and non-SP cells in NOD/SCID mice tumorigenicity. Furthermore, SP cells demonstrated a higher degree of drug resistance against trichostatin A (TSA) compared with that of non-SP and Hela cells. SP cells were also found to be more resistant against radiotherapy. Conclusions SP cells possess some characteristics of CSCs, namely high proliferation ability, chemoresistance and radioresistance, which may be helpful to elucidate novel targets for effective clinical treatments of cervical cancer in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
11. Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms.
- Author
-
Chengjian Jiang, Lan-Lan Wu, Gao-Chao Zhao, Pei-Hong Shen, Ke Jin, Zhen-Yu Hao, Shuang-Xi Li, Ge-Fei Ma, Feng-Feng Luo, Guo-Qing Hu, Wen-Long Kang, Xing-Mei Qin, You-Li Bi, Xian-Lai Tang, and Bo Wu
- Subjects
GENE expression ,MICROORGANISMS ,TRICARBOXYLIC acids ,CLONING ,AMINO acid metabolism - Abstract
Background: Fumarase catalyzes the reversible hydration of fumarate to L-malate and is a key enzyme in the tricarboxylic acid (TCA) cycle and in amino acid metabolism. Fumarase is also used for the industrial production of L-malate from the substrate fumarate. Thermostable and high-activity fumarases from organisms that inhabit extreme environments may have great potential in industry, biotechnology, and basic research. The marine environment is highly complex and considered one of the main reservoirs of microbial diversity on the planet. However, most of the microorganisms are inaccessible in nature and are not easily cultivated in the laboratory. Metagenomic approaches provide a powerful tool to isolate and identify enzymes with novel biocatalytic activities for various biotechnological applications. Results: A plasmid metagenomic library was constructed from uncultivated marine microorganisms within marine water samples. Through sequence-based screening of the DNA library, a gene encoding a novel fumarase (named FumF) was isolated. Amino acid sequence analysis revealed that the FumF protein shared the greatest homology with Class II fumarate hydratases from Bacteroides sp. 2_1_33B and Parabacteroides distasonis ATCC 8503 (26% identical and 43% similar). The putative fumarase gene was subcloned into pETBlue-2 vector and expressed in E. coli BL21(DE3)pLysS. The recombinant protein was purified to homogeneity. Functional characterization by high performance liquid chromatography confirmed that the recombinant FumF protein catalyzed the hydration of fumarate to form L-malate. The maximum activity for FumF protein occurred at pH 8.5 and 55°C in 5 mM Mg
2+ . The enzyme showed higher affinity and catalytic efficiency under optimal reaction conditions: Km = 0.48mM, Vmax = 827 μM/min/mg, and kcat /Km = 1900 mM/s. Conclusions: We isolated a novel fumarase gene, fumF, from a sequence-based screen of a plasmid metagenomic library from uncultivated marine microorganisms. The properties of FumF protein may be ideal for the industrial production of L-malate under higher temperature conditions. The identification of FumF underscores the potential of marine metagenome screening for novel biomolecules. [ABSTRACT FROM AUTHOR]- Published
- 2010
- Full Text
- View/download PDF
12. Role of hepatitis B surface antigen in the development of hepatocellular carcinoma: regulation of lymphoid enhancer-binding factor I.
- Author
-
Xiaochen Tian, Jinjun Li, Zhang-Mei Ma, Chao Zhao, Da-Fang Wan, and Yu-Mei Wen
- Subjects
HEPATITIS B ,CELL surface antigens ,LIVER cancer ,CANCER risk factors ,GENETIC regulation ,CELL culture ,CANCER cells ,CELL proliferation ,CANCER research ,MEDICAL research - Abstract
Background: There are around 350 million of hepatitis B surface antigen (HBsAg) carriers worldwide, and among them, high risk of developing hepatocellular carcinoma (HCC) has been identified by epidemiological studies. To date, the molecular role of HBsAg in HCC development has not been fully studied. We have previously reported that in cell cultures, HBsAg up-regulated the expression of lymphoid enhancer-binding factor 1 (LEF-1), a key component of the Wnt pathway. In this study we aimed to study this effect of HBsAg on LEF-1 in the development of HCC. Methods: Expression of HBsAg, LEF-1 and its downstream effector genes were compared among 30 HCCs, their peritumor tissue counterparts and 9 normal control liver tissues by quantitative real-time PCR. In addition, immunohistochemical staining studies on HBsAg and LEF-1 expression were conducted among these samples. Results: The expression of LEF-1 was compared between 13 HBsAg positive HCC tissues and 17 HBsAg negative HCC tissues. Simultaneous detection of LEF-1 and HBsAg was observed in HBsAg positive HCC tissues and, additionally, the simultaneous detection of HBsAg and LEF-1 was more pronounced in peritumor tissues, compared to that in the tumor tissues. The distribution of cellular LEF-1 in peritumor tissues was predominantly in the cytoplasm; while LEF-1 in the tumor tissues was located either exclusively in the nucleus or both in the nucleus and cytoplasm. By realtime PCR, the expression levels of LEF-1 downstream effector genes cyclin D1 and c-myc were higher in peritumor cells compared to that of the tumor cells. However, a 38 kDa truncated isoform of LEF-1, rather than the 55 kDa wild-type LEF-1, was significantly elevated in the HBsAg positive tumor cells. Conclusion: Data indicate that deregulation of the Wnt pathway by HBsAg occurred in HBVassociated HCCs, but was more pronounced in the peritumor cells. It is speculated that HBsAg could stimulate proliferation and functional modification of hepatocytes via LEF-1 through the Wnt pathway at the pre-malignant stage. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
13. The uterine expression of SEC63 gene is up-regulated at implantation sites in association with the decidualization during the early pregnancy in mice.
- Author
-
Ren-wei Su, Zhao-gui Sun, Yue-chao Zhao, Qiu-ju Chen, Zengming Yang, Run-sheng Li, and Jian Wang
- Subjects
GENE expression ,ENDOPLASMIC reticulum ,ORGANELLES ,PREGNANCY in animals ,IMMUNOHISTOCHEMISTRY ,PREGNANCY ,HUMAN embryo transfer - Abstract
Background: Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins. The aim of this study was to determine the expression pattern of SEC63 gene in mouse uterus during the early pregnancy. Methods: Real-time quantitative PCR and Western blot analyses were used to evaluate the alteration in levels of uterine SEC63 gene expression during the peri-implantation period in mice. Further, both in situ hybridization and immunohistochemical analyses were performed to examine the spatial localization of SEC63 gene expression in mouse uterine tissues. The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis. Statistical analysis was carried out using Tukey test. Results: Uterine SEC63 gene expression was up-regulated and predominantly localized in mouse decidual cells during days 5-8 of pregnancy. More interestingly, Sec63 protein was also detected in human decidua of 10-week pregnancy, whereas was not observed in human endometrial tissues both at proliferative and secretory phases of menstrual cycle. Conclusion: The pattern of SEC63 gene expression is consistent with a possible role for SEC63 in decidualization. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
14. Retraction Note: Comparison of the sagittal profiles among thoracic idiopathic scoliosis patients with different Cobb angles and growth potentials.
- Author
-
Bo Ran, Guo-you Zhang, Feng Shen, Jia-yu Chen, Ji-bin Wu, Feng-chao Zhao, Kai-jin Guo, Dun-yi Qi, Bing Zhou, Xiang-yang Chen, Xin-zhu Zhang, Yue-hua Qiao, and Ming Li
- Subjects
SCOLIOSIS - Abstract
A correction to the article "Comparison of the Sagittal Profiles Among Thoracic Idiopathic Scoliosis Patients With Different Cobb Angles and Growth Potentials" that was published in a 2014 issue is presented.
- Published
- 2015
- Full Text
- View/download PDF
15. Erratum: comparison of the sagittal profiles among thoracic idiopathic scoliosis patients with different Cobb angles and growth potentials.
- Author
-
Bo Ran, Xiang-yang Chen, Guo-you Zhang, Feng Shen, Jia-yu Chen, Ji-bin Wu, Feng-chao Zhao, Dun-yi Qiao, Bing Zhou, Xin-zhu Zhang, Yue-hua Qiao, Jun-hui Guan, Kai-jin Guo, and Ming Li
- Abstract
Correction After publication of this work [1], we noted that we inadvertently failed to include the complete list of all coauthors, and they were not listed in the correct order. In addition, we have provided updated Authors’ Contributions and Competing interests sections. We apologize for any inconvenience these oversights may have caused. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.