1. A novel biocatalyst for efficient production of 2-oxo-carboxylates using glycerol as the cost-effective carbon source
- Author
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Cuiqing Ma, Yujiao Wang, Binbin Sheng, Chunyu Yang, Yingxin Zhang, Chao Gao, Jingjing Meng, Ping Xu, and Tianyi Jiang
- Subjects
Glycerol ,2-Oxo-carboxylate ,Biodiesel ,biology ,Renewable Energy, Sustainability and the Environment ,Research ,Mutant ,Pseudomonas putida KT2440 ,Management, Monitoring, Policy and Law ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Combinatorial chemistry ,Pseudomonas putida ,NAD-independent lactate dehydrogenase ,chemistry.chemical_compound ,General Energy ,chemistry ,Biochemistry ,Biotransformation ,Biocatalysis ,Biofuel ,Lactate dehydrogenase ,Biocatalyst ,Biotechnology - Abstract
Background The surplus of glycerol has increased remarkably as a main byproduct during the biofuel’s production. Exploiting an alternative route for glycerol utilization is significantly important for sustainability of biofuels. Results A novel biocatalyst that could be prepared from glycerol for producing 2-oxo-carboxylates was developed. First, Pseudomonas putida KT2440 was reconstructed by deleting lldR to develop a mutant expressing the NAD-independent lactate dehydrogenases (iLDHs) constitutively. Then, the Vitreoscilla hemoglobin (VHb) was heterologously expressed to further improve the biotransformation activity. The reconstructed strain, P. putida KT2440 (ΔlldR)/pBSPPcGm-vgb, exhibited high activities of iLDHs when cultured with glycerol as the carbon source. This cost-effective biocatalyst could efficiently produce pyruvate and 2-oxobutyrate from dl-lactate and dl-2-hydroxybutyrate with high molar conversion rates of 91.9 and 99.8 %, respectively. Conclusions The process would not only be a promising alternative for the production of 2-oxo-carboxylates, but also be an example for preparation of efficient biocatalysts for the value-added utilization of glycerol.
- Published
- 2015