217 results on '"Fang WANG"'
Search Results
2. The roles of miRNA, lncRNA and circRNA in the development of osteoporosis
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Yang, Yang, Yujiao, Wang, Fang, Wang, Linhui, Yuan, Ziqi, Guo, Zhichen, Wei, Zirui, Wang, and Shengwang, Wang
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- 2020
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3. Phosphoserine phosphatase as a prognostic biomarker in patients with gastric cancer and its potential association with immune cells
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Ma-Yan Huang, Xiao-Yun Liu, Qiong Shao, Xu Zhang, Lei Miao, Xiao-Yan Wu, Yu-Xia Xu, Fang Wang, Hai-Yun Wang, Liang Zeng, and Ling Deng
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Poor prognosis ,Research ,Immune score ,Gastroenterology ,General Medicine ,RC799-869 ,Kaplan-Meier Estimate ,Diseases of the digestive system. Gastroenterology ,Prognosis ,Phosphoric Monoester Hydrolases ,PSPH ,Stomach Neoplasms ,Metabolic genes ,Biomarkers, Tumor ,Humans ,Gastric cancer ,Biomarkers - Abstract
Background Because of dismal prognosis in gastric cancer, identifying relevant prognostic factors is necessary. Phosphoserine phosphatase (PSPH) exhibits different expression patterns in many cancers and has been reported to affect the prognosis of patients with cancer. In this study, we examined the prognostic role of metabolic gene PSPH in gastric cancer based on the TCGA dataset and our hospital–based cohort cases. Methods We collected and analysed RNA-seq data of Pan-cancer and gastric cancer in the TCGA dataset and PSPH expression data obtained from immunohistochemical analysis of 243 patients with gastric cancer from Sun Yat-sen University cancer center. Further, Kaplan–Meier survival analysis and Cox analysis were used to assess the effect of PSPH on prognosis. The ESTIMATE and Cibersort algorithms were used to elucidate the relationship between PSPH and the abundance of immune cells using the TCGA dataset. Results We observed that PSPH expression displayed considerably high in gastric cancer and it was significantly associated with inferior prognosis (P = 0.043). Surprisingly, there was a significant relationship between lower immune scores and high expression of PSPH (P P = 0.046). The expression of PSPH significantly increased in activated memory CD4 T cells, resting NK cells and M0 macrophages (P = 0.037, Conclusions This study highlighted that PSPH influences the prognosis of patients with gastric cancer, and this is associated with the infiltration of tumour immune cells, indicating that PSPH may be a new immune-related target for treating gastric cancer.
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- 2022
4. Translation and validation of modified dental anxiety scale based on adult Taiwan population
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Chia-Shu Lin, Chen-Yi Lee, Shih-Yun Wu, Li-Ling Chen, Kun-Tsung Lee, Min-Ching Wang, and Tze-Fang Wang
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Adult ,Male ,Psychometrics ,Research ,Taiwan ,Pain ,Reproducibility of Results ,RK1-715 ,Fear ,stomatognathic diseases ,stomatognathic system ,Dentistry ,Surveys and Questionnaires ,Avoidance ,Dental Anxiety ,Humans ,Female ,General Dentistry - Abstract
Background Dental anxiety is associated with negative experiences of dental treatment and dental-visiting behavior. The Modified Dental Anxiety Scale (MDAS) is widely used for assessing dental anxiety. The study aims to establish the psychometric properties of a Chinese version of the MDAS based on the Taiwan sample (i.e., T-MDAS). Methods The T-MDAS and dental-visiting behavior and experience were assessed for 402 adult subjects recruited from community and clinical sites. The following psychometric properties were assessed: (a) internal consistency, (b) temporal stability, (c) criterion-related validity (i.e., the association with the score of Index of Dental Anxiety and Fear, IDAF-4C), (d) discrimination validity (i.e., the difference in scores between the subjects with and without a habit of a regular dental visit, and (e) the construct validity from a confirmatory factor analysis (CFA). Results. The T-MDAS showed good internal consistency (Cronbach’s α = 0.88) and temporal stability (ρ = 0.69, p p Conclusions The T-MDAS is a valid tool for assessing adult dental anxiety. The score is highly associated with dental-visiting behavior and experience of dental patients.
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- 2021
5. Effect of endometrial thickness changes on clinical pregnancy rates after progesterone administration in a single frozen-thawed euploid blastocyst transfer cycle using natural cycles with luteal support for PGT-SR- and PGT-M-assisted reproduction: a retrospective cohort study
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Wenbin Niu, En-tong Yang, Mingzhu Huo, Fang Wang, Hao Shi, Jingdi Li, Ziqi Jin, Hui Song, Zhiqin Bu, and Yile Zhang
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QH471-489 ,Pregnancy Rate ,Endometrium ,Miscarriage ,Cohort Studies ,Endocrinology ,Pregnancy ,Luteal support ,Progesterone ,Reproduction ,Blastocyst Transfer ,Ultrasound ,Pregnancy Outcome ,Obstetrics and Gynecology ,Organ Size ,medicine.anatomical_structure ,Treatment Outcome ,Endometrial thickness change ratio ,embryonic structures ,Natural cycle ,Female ,Live birth ,Clinical pregnancy rates ,Adult ,China ,Reproductive Techniques, Assisted ,Hormone Replacement Therapy ,Euploid blastocyst transfer ,Fertilization in Vitro ,Luteal Phase ,Andrology ,medicine ,Humans ,Blastocyst ,Embryo Implantation ,Retrospective Studies ,Cryopreservation ,business.industry ,Research ,Transvaginal ultrasound ,Odds ratio ,Gynecology and obstetrics ,medicine.disease ,Embryo Transfer ,Reproductive Medicine ,RG1-991 ,sense organs ,business ,Developmental Biology - Abstract
Background To investigate whether the endometrial thickness change ratio from the progesterone administration day to the blastocyst transfer day is associated with pregnancy outcomes in a single frozen-thawed euploid blastocyst transfer cycle. Methods All patients used natural cycles with luteal support for endometrial preparation and selected a single euploid blastocyst for transfer after a biopsy for preimplantation genetic testing. The endometrial thickness was measured by transvaginal ultrasound on the progesterone administration day and the transfer day, the change in endometrial thickness was measured, and the endometrial thickness change ratio was calculated. According to the change rate of endometrial thickness, the patients were divided into three groups: the endometrial thickness compaction group, endometrial thickness non-change group and endometrial thickness expansion group. Among them, the endometrial thickness non-change and expansion groups were combined into the endometrial thickness noncompaction group. Results Ultrasound images of the endometrium in 219 frozen-thawed euploid blastocyst transfer cycles were evaluated. The clinical pregnancy rate increased with the increase in endometrial thickness change ratio, while the miscarriage rate and live birth rate were comparable among the groups. The multiple logistic regression results showed that in the fully adjusted model a higher endometrial thickness change ratio (per 10%) was associated with a higher clinical pregnancy rate (adjusted odds ratio [aOR] 1.29; 95% confidence interval [CI], 1.01–1.64; P = .040). Similarly, when the patients were divided into three groups according to the change rate of endometrial thickness, the endometrial thickness noncompaction group had a significant positive effect on the clinical pregnancy rate compared with the endometrial thickness compaction group after adjusting for all covariates. Conclusions In frozen-thawed euploid blastocyst transfer cycles in which the endometrium was prepared by natural cycles with luteal support, the clinical pregnancy rate was higher in cycles without endometrial compaction after progesterone administration.
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- 2021
6. Obesity management in polycystic ovary syndrome: disparity in knowledge between obstetrician-gynecologists and reproductive endocrinologists in China
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Qingmei Zheng, Ai-Jun Sun, Wei Wang, Yan-Fang Wang, Xiaoqin Zeng, Ying Zou, Zhangyun Tan, Rui-Lin Ma, Yan Deng, Ying Feng, Han Dong, and Yinqing Zhao
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Adult ,Male ,medicine.medical_specialty ,China ,Health Knowledge, Attitudes, Practice ,Waist ,endocrine system diseases ,Adolescent ,Attitude of Health Personnel ,Endocrinology, Diabetes and Metabolism ,Specialty ,Oral glucose tolerance test ,Diseases of the endocrine glands. Clinical endocrinology ,Body Mass Index ,Young Adult ,Obstetrics and gynaecology ,Internal medicine ,Diabetes mellitus ,Surveys and Questionnaires ,medicine ,Humans ,Obesity ,Practice Patterns, Physicians' ,Life Style ,Abdominal obesity ,Polycystic ovary syndrome ,Metabolic Syndrome ,business.industry ,Reproduction ,nutritional and metabolic diseases ,General Medicine ,Middle Aged ,RC648-665 ,medicine.disease ,Prognosis ,Polycystic ovary ,Metformin ,Obesity Management ,Lipid profile ,Obstetrics ,Endocrinologists ,Gynecology ,Female ,medicine.symptom ,business ,Body mass index ,Research Article ,Follow-Up Studies - Abstract
Background Obesity is associated with the development of polycystic ovary syndrome (PCOS) and contributes substantially to metabolic abnormalities in women with PCOS. The study aimed to describe and compare the practices of physicians in the diagnosis, evaluation, and treatment of obesity in patients with PCOS. Methods Reproductive endocrinologists (Repro-Endo) and obstetrician-gynecologists (non-reproductive medicine specialty, OB-Gyn) in China participated in a survey, and their responses were analyzed using χ2 tests, Fisher exact tests, and multivariable logistic regression analysis. Results The study analyzed 1318 survey responses (85.8% OB-Gyn; 97.3% women). Body mass index was the most common diagnostic criterion for obesity; only 1.3% of participants measured waist circumference to identify abdominal obesity. More Repro-Endo participants (25% of all participants) enquired about the psychological problems of patients with obesity than OB-Gyn participants, and 42.5% of participants reported ordering both a lipid profile and oral glucose tolerance test (OGTT) for patients with obesity and PCOS. Multivariable analysis, that included physician’s specialty, age, hospital grade, and number of patients with PCOS seen annually, revealed that OB-Gyn participants were less likely to order OGTT (OR, 0.3; 95% CI, 0.2–0.4) and lipid profile (OR, 0.2; 95% CI, 0.1–0.3) than Repro-Endo participants. The most common treatments for patients with PCOS were lifestyle modification (> 95%) and metformin (> 80%). More Repro-Endo participants prescribed metformin at a dose of 1.5 g/day compared with OB-Gyn (47.6% vs. 26.3%), and more OB-Gyn participants reported being unclear about the appropriate dosage of metformin for patients with obesity and PCOS (8.9% vs. 1.6%). Conclusion Our survey identified knowledge gaps in metabolic screening for patients with obesity and PCOS and a disparity in the evaluation and treatment of obesity in PCOS among different specialties. Similarly, it highlights the need to improve obesity management education for physicians caring for women with PCOS.
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- 2021
7. Ensemble machine learning approach for screening of coronary heart disease based on echocardiography and risk factors
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Huolan Zhu, Chenguang Yang, Yongkai Chen, Yi Li, Wenxuan Zhong, Jingyi Zhang, Hui-Min Cheng, and Fang Wang
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Preventive screening ,FOS: Computer and information sciences ,Computer Science - Machine Learning ,Computer science ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Health Informatics ,Speckle tracking echocardiography ,Machine Learning (stat.ML) ,Coronary Disease ,030204 cardiovascular system & hematology ,Machine learning ,computer.software_genre ,Health informatics ,Machine Learning (cs.LG) ,Machine Learning ,03 medical and health sciences ,0302 clinical medicine ,Statistics - Machine Learning ,Risk Factors ,Ensemble learning ,Humans ,Mass Screening ,Screening tool ,030212 general & internal medicine ,Multiple classification ,business.industry ,Health Policy ,Reproducibility of Results ,Classification ,Coronary heart disease ,Computer Science Applications ,Echocardiography ,Classification methods ,Artificial intelligence ,business ,computer ,Research Article - Abstract
Background: Extensive clinical evidence suggests that a preventive screening of coronary heart disease (CHD) at an earlier stage can greatly reduce the mortality rate. We use 64 two-dimensional speckle tracking echocardiography (2D-STE) features and seven clinical features to predict whether one has CHD. Methods: We develop a machine learning approach that integrates a number of popular classification methods together by model stacking, and generalize the traditional stacking method to a two-step stacking method to improve the diagnostic performance. Results: By borrowing strengths from multiple classification models through the proposed method, we improve the CHD classification accuracy from around 70% to 87.7% on the testing set. The sensitivity of the proposed method is 0.903 and the specificity is 0.843, with an AUC of 0.904, which is significantly higher than those of the individual classification models. Conclusions: Our work lays a foundation for the deployment of speckle tracking echocardiography-based screening tools for coronary heart disease., 30 pages, 5 figures, 5 tables
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- 2021
8. GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma
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Min Luo, Qi-xiang Rong, Fang Wang, Hong Zhang, Sainan An, Hui-qiang Huang, Yi Hu, Shaocong Wu, Zhixing Guo, and Liwu Fu
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0301 basic medicine ,PD-L1 ,Cancer Research ,medicine.medical_treatment ,Cell ,Immunopotentiator ,ENKTL ,B7-H1 Antigen ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Immune system ,Adjuvants, Immunologic ,medicine ,JAK/STAT pathway ,T-cell lymphoma ,Animals ,Humans ,RC254-282 ,biology ,Research ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Granulocyte-Macrophage Colony-Stimulating Factor ,GM-CSF ,Natural killer T cell ,medicine.disease ,Lymphoma ,Up-Regulation ,Lymphoma, Extranodal NK-T-Cell ,030104 developmental biology ,Cytokine ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,Molecular Medicine ,Tumor Escape - Abstract
Background Granulocyte-macrophage colony stimulating factor (GM-CSF) is a cytokine that is used as an immunopotentiator for anti-tumor therapies in recent years. We found that some of the extranodal natural killer/T cell lymphoma (ENKTL) patients with the treatment of hGM-CSF rapidly experienced disease progression, but the underlying mechanisms remain to be elucidated. Here, we aimed to explore the mechanisms of disease progression triggered by GM-CSF in ENKTL. Methods The mouse models bearing EL4 cell tumors were established to investigate the effects of GM-CSF on tumor growth and T cell infiltration and function. Human ENKTL cell lines including NK-YS, SNK-6, and SNT-8 were used to explore the expression of programmed death-ligand 1 (PD-L1) induced by GM-CSF. To further study the mechanisms of disease progression of ENKTL in detail, the mutations and gene expression profile were examined by next-generation sequence (NGS) in the ENKTL patient’s tumor tissue samples. Results The mouse-bearing EL4 cell tumor exhibited a faster tumor growth rate and poorer survival in the treatment with GM-CSF alone than in treatment with IgG or the combination of GM-CSF and PD-1 antibody. The PD-L1 expression at mRNA and protein levels was significantly increased in ENKTL cells treated with GM-CSF. STAT5A high-frequency mutation including p.R131G, p.D475N, p.F706fs, p.V707E, and p.S710F was found in 12 ENKTL cases with baseline tissue samples. Importantly, STAT5A-V706fs mutation tumor cells exhibited increased activation of STAT5A pathway and PD-L1 overexpression in the presence of GM-CSF. Conclusions These findings demonstrate that GM-CSF potentially triggers the loss of tumor immune surveillance in ENKTL patients and promotes disease progression, which is associated with STAT5 mutations and JAK2 hyperphosphorylation and then upregulates the expression of PD-L1. These may provide new concepts for GM-CSF application and new strategies for the treatment of ENKTL.
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- 2021
9. Silencing SHMT2 inhibits the progression of tongue squamous cell carcinoma through cell cycle regulation
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Hongshi Cai, Fang Wang, Fan Song, Jinsong Hou, Yadong Zhang, and Yan Liao
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Cancer Research ,Gene set enrichment analysis ,Cell ,Biology ,Cell cycle ,Flow cytometry ,Small hairpin RNA ,03 medical and health sciences ,0302 clinical medicine ,Weighted gene co-expression network analysis ,Genetics ,medicine ,Gene silencing ,RC254-282 ,030304 developmental biology ,0303 health sciences ,Gene knockdown ,medicine.diagnostic_test ,QH573-671 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Transfection ,Survival analysis ,SHMT2 ,medicine.anatomical_structure ,Oncology ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Cytology ,Primary Research ,Tongue squamous cell carcinoma - Abstract
Background Serine hydroxymethyltransferase 2 (SHMT2) is a vital metabolic enzyme in one carbon metabolism catalyzing the conversion of serine to glycine, which has been reported to play a crucial role in the progression of tumors. However, its function in tongue squamous cell carcinoma (TSCC) remains unclear. Methods SHMT2 expression was analyzed using samples in online databases, and was assessed through immunohistochemistry staining of collected clinical specimens. The correlation between SHMT2 expression and the cell cycle was predicted through bioinformatic analysis, including weighted gene co-expression network analysis (WGCNA) and gene set enrichment analysis (GSEA). After transfection with siRNA, CCK8 assay, Edu staining, flow cytometry, trans-well assay, and wound healing experiments were performed to verify the functional role of SHMT2 in vitro. A stable cell line with SHMT2 silencing was established to detect the oncogenic function of SHMT2 in vivo. Results The expression of SHMT2 was up-regulated in TSCC tissues and cell lines compared with normal groups, and highly expressed SHMT2 significantly indicated a poorer clinical outcome for TSCC patients. Bioinformatic analysis found that high expression of SHMT2 was closely related with biologic process including cell cycle and cell cycle G1/S transition. Down regulating of SHMT2 significantly suppressed the proliferation, invasive and migrative ability of TSCC cells, and induced the prolongation of the G1 phase of the cell cycle in vitro. Furthermore, western blot showed that cell cycle-related regulators such as cyclin-dependent kinase 4 (CDK4) and cyclinD1 expression levels were decreased, while the expression levels of the cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1 were increased after SHMT2 knockdown. Silencing SHMT2 in the HN6 cell line using short hairpin RNA also impeded tumor growth in vivo. Conclusions Overexpression of SHMT2 in TSCC indicated low survival rates, and was associated with aggressive behaviors of TSCC. It was also found to be involved in cell cycle regulation of TSCC cells. SHMT2 may serve as a novel prognostic indicator of TSCC.
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- 2021
10. Culture-dependent and culture-independent characterization of bacterial community diversity in different types of sandy lands: the case of Minqin County, China
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Yaolong Huang, Fang Wang, Yali Wei, Hongmei Sheng, Jiangli Gao, and Wei Ren
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Microbiology (medical) ,China ,Firmicutes ,Sandy land ,lcsh:QR1-502 ,Biodiversity ,Microbiology ,lcsh:Microbiology ,Actinobacteria ,03 medical and health sciences ,Minqin Desert ,Sand ,RNA, Ribosomal, 16S ,Desertification ,Soil Microbiology ,Haloxylon ammodendron ,0303 health sciences ,Rhizosphere ,Bacteriological Techniques ,biology ,Bacteria ,030306 microbiology ,Ecology ,Bacteroidetes ,04 agricultural and veterinary sciences ,biology.organism_classification ,Culture-dependent method ,Culture-independent method ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Proteobacteria ,Desert Climate ,Bacteria community ,Acidobacteria ,Research Article - Abstract
Background Minqin is suffering from a serious desertification, whereas the knowledge about its bacterial community is limited. Herein, based on Nitraria tangutorum and Haloxylon ammodendron from Minqin, the bacterial community diversities in fixed sandy land, semi-fixed sandy land and shifting sandy land were investigated by combining with culture-dependent and culture-independent methods. Results Minqin stressed with high salinity and poor nutrition is an oligotrophic environment. Bacterial community in Minqin was shaped primarily by the presence of host plants, whereas the type of plant and sandy land had no marked effect on those, which displayed a better survival in the rhizospheres of N. tangutorum and H. ammodendron. The dominant groups at phyla level were Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, Planctomycetes, Chloroflexi, Acidobacteria and Candidate_division_TM7. The abundance of Firmicutes with ability of desiccation-tolerance was significantly higher in harsh environment, whereas Bacteroidetes were mainly distributed in areas with high nutrient content. The abundances of Proteobacteria and Bacteroidetes were relatively high in the rhizospheres of N. tangutorum and H. ammodendron, which had more plant-growth promoting rhizobacteria. A large number of Actinobacteria were detected, of which the most abundant genus was Streptomyces. The physicochemical factors related to the diversity and distribution of the bacterial community were comprehensively analyzed, such as pH, electrical conductivity, soil organic matter, C/N and sand, and the results indicated that Minqin was more suitable for the growth of N. tangutorum, which should be one of most important sand-fixing plants in Minqin. Conclusions The bacterial community diversities in different types of sandy lands of Minqin were comprehensively and systematically investigated by culture-dependent and culture-independent approaches, which has a great significance in maintaining/restoring biological diversity.
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- 2021
11. Exosomes from human umbilical cord mesenchymal stem cells attenuate the inflammation of severe steroid-resistant asthma by reshaping macrophage polarization
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Guoqiang Wang, He Pan, Jinrong Zhang, Bing Dong, Chao Wang, Jing Zhang, Xu Zuo, Xiaoping Guo, Yinuo Gu, Alan Chen Yu Hsu, and Fang Wang
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0301 basic medicine ,Macrophage polarization ,Medicine (miscellaneous) ,Inflammation ,Severe steroid-resistant asthma ,Exosomes ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Exosome ,Flow cytometry ,Umbilical Cord ,lcsh:Biochemistry ,03 medical and health sciences ,Mice ,Phosphatidylinositol 3-Kinases ,0302 clinical medicine ,Medicine ,Animals ,Humans ,lcsh:QD415-436 ,PI3K/AKT/mTOR pathway ,Mesenchymal stem cell ,lcsh:R5-920 ,medicine.diagnostic_test ,business.industry ,Research ,Macrophages ,Mesenchymal Stem Cells ,Cell Biology ,Asthma ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,Quality of Life ,Molecular Medicine ,Tumor necrosis factor alpha ,Steroids ,Stem cell ,medicine.symptom ,lcsh:Medicine (General) ,business - Abstract
Background Severe, steroid-resistant asthma (SSRA) is a serious clinical problem in asthma management. Affected patients have severe clinical symptoms, worsened quality of life, and do not respond to steroid, a mainstay steroid treatment of asthma. Thus, effective therapies are urgently needed. Exosomes derived from mesenchymal stem cell (MSC-Exo) has become attractive candidates for the lung inflammatory diseases through its immunomodulatory effects. In this study, we explored the therapeutic effects of MSC-Exo in SSRA and identified the therapeutic mechanism of MSC-Exo. Method Exosomes from human umbilical cord mesenchymal stem cell (hUCMSC) were isolated and characterized by transmission electron microscopy, nanoparticle tracking analysis and flow cytometry analysis. Effects of MSC-Exo on airway hyper responsiveness (AHR), inflammation, histopathology, and macrophage polarization in SSRA in mice were evaluated. Systematic depletion of macrophages determined the role of macrophages in the therapeutic effect of SSRA in mice. LPS-stimulated RAW 264.7 cell model was constructed to determine the underlying mechanism of MSC-Exo on macrophage polarization. qRT-PCR, Western blotting, immunofluorescence, and flow cytometry were performed to evaluate the expression of M1 or M2 markers. Tandem mass tags (TMT)-labeled quantitative proteomics were applied to explore the central protein during the regulation effect of MSC-Exo on macrophage polarization. Knockdown and overexpression of TRAF1 were used to further clarify the role of the central protein on macrophage polarization. Result We successfully isolated and characterized exosomes from hUCMSCs. We verified that the intratracheal administration of MSC-Exo reversed AHR, histopathology changes, and inflammation in SSRA mice. Systematic depletion of macrophages weakened the therapeutic effect of MSC-Exo. We found that MSC-Exo treatment inhibited M1 polarization and promoted M2 polarization in LPS-stimulated RAW 264.7 cells. Subsequently, tumor necrosis factor receptor-associated factor 1 (TRAF1) was determined as the central protein which may be closely related to the regulation of macrophage polarization from TMT-labeled quantitative proteomics analysis. Knockdown and overexpression of TRAF1 demonstrated that the effect of MSC-Exo treatment on macrophage polarization, NF-κB and PI3K/AKT signaling was dependent on TRAF1. Conclusion MSC-Exo can ameliorate SSRA by moderating inflammation, which is achieved by reshaping macrophage polarization via inhibition of TRAF1.
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- 2021
12. MEDALT: single-cell copy number lineage tracing enabling gene discovery
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Shaoheng Liang, Li Ding, Jinzhuang Dou, Nicholas Navin, Ruli Gao, Jincheng Han, Darlan Conterno Minussi, Ken Chen, Vakul Mohanty, Qihan Wang, and Fang Wang
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Lineage (genetic) ,lcsh:QH426-470 ,Cell ,Gene Dosage ,Computational biology ,Biology ,Lineage tracing ,Evolution, Molecular ,Breast cancer ,Phylogenetics ,Genetic algorithm ,scRNA-seq ,medicine ,Humans ,Genetic Predisposition to Disease ,RNA-Seq ,Gene ,lcsh:QH301-705.5 ,Genetic Association Studies ,Single-cell ,scDNA-seq ,Research ,Driver discovery ,Computational Biology ,High-Throughput Nucleotide Sequencing ,Copy number alteration ,medicine.disease ,Aneuploidy ,Human genetics ,lcsh:Genetics ,medicine.anatomical_structure ,Tumor evolution ,lcsh:Biology (General) ,Genetic Fitness ,Single-Cell Analysis ,Algorithms ,Software - Abstract
We present a Minimal Event Distance Aneuploidy Lineage Tree (MEDALT) algorithm that infers the evolution history of a cell population based on single-cell copy number (SCCN) profiles, and a statistical routine named lineage speciation analysis (LSA), whichty facilitates discovery of fitness-associated alterations and genes from SCCN lineage trees. MEDALT appears more accurate than phylogenetics approaches in reconstructing copy number lineage. From data from 20 triple-negative breast cancer patients, our approaches effectively prioritize genes that are essential for breast cancer cell fitness and predict patient survival, including those implicating convergent evolution.The source code of our study is available at https://github.com/KChen-lab/MEDALT.
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- 2021
13. Correction to: Genetic analysis of Wnt/PCP genes in neural tube defects
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Hongyan Wang, Ting Zhang, Rui Peng, Xuanye Cao, Zhongzhong Chen, Yihua Bao, Yunping Lei, Yufang Zheng, Fang Wang, and Richard H. Finnell
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lcsh:Internal medicine ,lcsh:QH426-470 ,Computational biology ,Biology ,Genetic analysis ,Text mining ,Gene expression ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,Neural Tube Defects ,lcsh:RC31-1245 ,Child ,Gene ,Genetics (clinical) ,business.industry ,Neural tube ,Wnt signaling pathway ,Correction ,Cell Polarity ,Cadherins ,Human genetics ,Wnt Proteins ,lcsh:Genetics ,medicine.anatomical_structure ,HEK293 Cells ,Mutation ,DNA microarray ,business ,HeLa Cells - Abstract
Mouse homozygous mutants in Wnt/planar cell polarity (PCP) pathway genes have been shown to cause neural tube defects (NTDs) through the disruption of normal morphogenetic processes critical to neural tube closure (NTC). Knockout mice that are heterozygotes of single PCP genes likely fail to produce NTD phenotypes, yet damaging variants detected in human NTDs are almost always heterozygous, suggesting that other deleterious interacting variants are likely to be present. Nonetheless, the Wnt/PCP pathway remains a genetic hotspot. Addressing these issues is essential for understanding the genetic etiology of human NTDs.We performed targeted next-generation sequencing (NGS) on 30 NTD-predisposing Wnt/PCP pathway genes in 184 Chinese NTD cases. We subsequently replicated our findings for the CELSR1 gene in an independent cohort of 292 Caucasian NTD samples from the USA. Functional validations were confirmed using in vitro assays.CELSR1, CELSR2 and CELSR3 genes were significantly clustered with rare driver coding mutations (q-value 0.05) demonstrated by OncodriveCLUST. During the validation stage, the number of rare loss of function (LoF) variants in CELSR1 was significantly enriched in NTDs compared with the LoF counts in the ExAC database (p 0.001). Functional studies indicated compound heterozygote variants of CELSR2 p.Thr2026Met and DVL3 p.Asp403Asn result in down regulation of PCP signals.These data indicate rare damaging variants of the CELSR genes, identified in ~ 14% of NTD cases, are expected to be driver genes in the Wnt/PCP pathway. Compound damaging variants of CELSR genes and other Wnt/PCP genes, which were observed in 3.3% of the studied NTD cohort, are also expected to amplify these effects at the pathway level.
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- 2021
14. Familial oral lichen planus in a 3-year-old boy: a case report with eight years of follow-up
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Fang Wang, Jing Zhang, Ya-Qin Tan, and Gang Zhou
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Adult ,Male ,medicine.medical_specialty ,Rb-bFGF ,Mucocutaneous zone ,Family history ,Case Report ,Tongue Diseases ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Tongue ,medicine ,Animals ,Humans ,Pediatric population ,Oral mucosa ,Child ,General Dentistry ,business.industry ,Lichen Planus ,Mouth Mucosa ,Family aggregation ,medicine.disease ,Dermatology ,stomatognathic diseases ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Child, Preschool ,Reticular connective tissue ,Oral lichen planus ,Oral and maxillofacial surgery ,Cattle ,business ,Follow-Up Studies ,Lichen Planus, Oral - Abstract
Background Oral lichen planus (OLP) is a chronic mucocutaneous disease characterized by adult predominance and a prolonged course. However, it is rare in the pediatric population with familial aggregation. Case presentation A 3-year-old boy presented with pain and irritation on the oral mucosa while contacting spicy food for 2 months. Oral examination showed widespread whitish reticular and papular lesions on the lips, the dorsum of the tongue, and bilateral buccal mucosa, with diffuse erosions covered with pseudomembrane on the buccal mucosa. The boy’s parents were examined to exhibit white reticular and plaque-like lesions on their oral mucosa. The three patients were clinically diagnosed as affected by OLP and histopathologically confirmed. The boy underwent topical treatment with recombinant bovine basic fibroblast growth factor (rb-bFGF) gel, and oral lesions gradually resolved and healed. Neither of his parents received treatment. During the subsequent follow-ups, none of three patients underwent any medical treatment. Fortunately, their lesions had almost faded over 8 years. Conclusions Our case emphasizes that pediatric OLP should be recorded with family history. Besides, long-term periodic follow-up is recommended in pediatric patients with OLP for monitoring any changes in lesions.
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- 2020
15. Association of serum creatinine with aortic arch calcification in middle-aged and elderly adults: an observational cross-sectional study from China.
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Feifei Zhang, Nannan Hao, Lei Wang, Guoming Sun, Xiaoke Feng, Chunjian Li, Wenfeng Tan, Fang Wang, Zhang, Feifei, Hao, Nannan, Wang, Lei, Sun, Guoming, Feng, Xiaoke, Li, Chunjian, Tan, Wenfeng, and Wang, Fang
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THORACIC aorta ,MIDDLE-aged persons ,CREATININE ,CALCIFICATION ,LOGISTIC regression analysis - Abstract
Background and Aims: Vascular calcification (VC) is a strong predictor of cardiovascular events and all-cause mortality in cardiovascular diseases (CVD). Renal dysfunction is closely related to VC. Serum creatinine, as an important indicator of renal function in chronic kidney disease (CKD), is closely associated with increased VC. Here, to explore the potential role of serum creatinine in CVD, we examined the association between serum creatinine level and aortic arch calcification (AAC) presence in a larger general population.Methods: A total of 9067 participants aged > 45 years were included in this study. All participants underwent postero-anterior chest X-ray examination to diagnose AAC. According to the distribution characteristics, serum creatinine levels in male and female were divided into tertiles respectively. Univariate and multivariate logistic regression analysis were used to analyze the association between aortic calcification and serum creatinine.Results: Participants included 3776 men and 5291 women, and 611 and 990 AAC were detected, respectively. Serum creatinine level in the female AAC group was significantly higher than that in the non-AAC group (p < 0.001), while there was no significant difference in male serum creatinine between the two groups (p = 0.241). After logistic regression analysis excluded confounding factors, with the first tertile of serum creatinine as the reference, multivariable-adjusted ORs and 95% CIs of the second and the highest tertile of female and male were 1.045 (0.856-1.276), 1.263 (1.036-1.539); 0.953 (0.761-1.193), 0.948 (0.741-1.198), respectively.Conclusion: Elevated serum creatinine levels are independently associated with higher AAC incidence in female aged > 45 years old. Measuring serum creatinine levels may assist the early screening individuals at high risk of developing CVD. And higher attention should be given to female's serum creatinine levels in daily clinical practice. [ABSTRACT FROM AUTHOR]- Published
- 2022
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16. Association between diabetes mellitus and health-related quality of life among patients with chronic kidney disease: results from the Chinese Cohort Study of Chronic Kidney Disease (C-STRIDE)
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Li Chen, Jinwei Wang, Xiaoyan Huang, Fang Wang, Wei Liang, Yan He, Yumei Liao, Luxia Zhang, Minghui Zhao, Zuying Xiong, and on behalf of the C-STRIDE study group
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Adult ,Male ,medicine.medical_specialty ,China ,Health-related quality of life ,Population ,Renal function ,030209 endocrinology & metabolism ,Disease ,Comorbidity ,030204 cardiovascular system & hematology ,lcsh:Computer applications to medicine. Medical informatics ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Quality of life ,Internal medicine ,Chronic kidney disease ,Prevalence ,Medicine ,Humans ,Estimated glomerular filtration rate ,Prospective Studies ,Stage (cooking) ,Renal Insufficiency, Chronic ,education ,Aged ,education.field_of_study ,business.industry ,Research ,Public Health, Environmental and Occupational Health ,General Medicine ,Middle Aged ,medicine.disease ,Case-Control Studies ,Quality of Life ,lcsh:R858-859.7 ,Female ,business ,Kidney disease ,Cohort study - Abstract
Background The prevalence of diabetes mellitus (DM) among patients with chronic kidney disease (CKD) has been increasing in recent years in China. This study aimed to evaluate the association between DM and health-related quality of life (HRQOL) in patients with CKD. Methods In our study, participants with CKD stage 1 to 4 from 39 centers in China were screened and enrolled. The Kidney Disease Quality of Life (KDQOL™-36) questionnaire was used to assess HRQOL. Participants were divided into a diabetic group and a non-diabetic group. Demographic data, clinical data, and HRQOL scores were compared between the two groups. Multivariable robust regression was used to analyze the factors related to HRQOL in CKD patients. Results A population of 2742 CKD patients was included in this study. CKD patients with DM were older and had lower education level, longer treatment periods and a higher prevalence of cardiovascular disease than CKD patients without DM (P P < 0.05). DM was negatively correlated with the symptoms and problems (regression coefficient for log transformed [175-score] = 0.010) and the SF-12 physical function dimension (regression coefficient = − 2.18) (all P Conclusion HRQOL of diabetic patients with CKD was worse than that of non-diabetic patients with CKD. DM was an independent and negative factor affecting HRQOL in patients with CKD.
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- 2020
17. Improved clinical outcomes of preimplantation genetic testing for aneuploidy using MALBAC-NGS compared with MDA-SNP array
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Fang Wang, Wenyan Song, Wenbin Niu, Jiawei Xu, Ying Li, Gang Li, Linlin Wang, Hao Shi, Haixia Jin, and Yingpu Sun
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Adult ,Male ,medicine.medical_specialty ,China ,Pregnancy Rate ,Aneuploidy ,Single-nucleotide polymorphism ,Fertilization in Vitro ,Single Center ,lcsh:Gynecology and obstetrics ,Polymorphism, Single Nucleotide ,Miscarriage ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Next generation sequencing ,Medicine ,SNP ,Humans ,Genetic Testing ,lcsh:RG1-991 ,Preimplantation Diagnosis ,030304 developmental biology ,Genetic testing ,Retrospective Studies ,0303 health sciences ,030219 obstetrics & reproductive medicine ,medicine.diagnostic_test ,business.industry ,Obstetrics ,Preimplantation genetic testing for aneuploidy ,MALBAC ,Pregnancy Outcome ,Obstetrics and Gynecology ,High-Throughput Nucleotide Sequencing ,medicine.disease ,Embryo Transfer ,Single nucleotide polymorphism ,Abortion, Spontaneous ,Blastocyst ,Karyotyping ,Female ,business ,SNP array ,Research Article - Abstract
Background To assess whether preimplantation genetic testing for aneuploidy with next generation sequencing (NGS) outweighs single nucleotide polymorphism (SNP) array in improving clinical outcomes. Methods A retrospective analysis of the clinical outcomes of patients who underwent PGT-A treatment in a single center from January 2013 to December 2017.A total of 1418 couples who underwent PGT-A treatment were enrolled, of which 805 couples used NGS for PGT-A, while the remaining 613 couples used SNP array for PGT-A. Clinical pregnancy rate, miscarriage rate and healthy baby rate were compared between the MALBAC-NGS-PGT-A and MDA-SNP-PGT-A groups. Results After testing karyotypes of 5771 biopsied blastocysts, 32.2% (1861/5771) were identified as chromosomally normal, while 67.8% were chromosomally abnormal. In terms of clinical outcomes, women in the MALBAC-NGS-PGT-A group had a significantly higher clinical pregnancy rate (50.5% vs 41.7%, p = 0.002) and healthy baby rate (39.6% vs 31.4%, p = 0.003), and a lower miscarriage rate (15.5% vs 22.8%, p = 0.036). Conclusion This is the largest study reporting the extensive application of NGS-based PGT-A, whilst comparing the clinical outcomes of MALBAC-NGS-PGT-A and MDA-SNP-PGT-A. The results provide greater evidence supporting the wider use of NGS in PGT-A, not only for its lower cost but also for its improved clinical outcomes compared to SNP-based PGT-A.
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- 2020
18. Enhanced sugar accumulation and regulated plant hormone signalling genes contribute to cold tolerance in hypoploid Saccharum spontaneum
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Hong‐Li Yang, Qing-Hui Yang, Xing-Hua Yu, Tian-Ju Wang, Chun‐Fang Wang, Yang Yang, and Xian-Hong Wang
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lcsh:QH426-470 ,lcsh:Biotechnology ,Clone (cell biology) ,Cold tolerance ,chemistry.chemical_compound ,Plant Growth Regulators ,lcsh:TP248.13-248.65 ,Botany ,Genetics ,Sugar accumulation ,Sugar ,Saccharum spontaneum ,biology ,Epidermis (botany) ,food and beverages ,Sugarcane ,Hormonal change ,biology.organism_classification ,Bulliform cell ,Trehalose ,Saccharum ,lcsh:Genetics ,Plant Breeding ,chemistry ,Hypoploidy ,Plant hormone ,Ploidy ,RNA-seq ,Sugars ,Transcriptome ,Biotechnology ,Research Article - Abstract
Background Wild sugarcane Saccharum spontaneum plants vary in ploidy, which complicates the utilization of its germplasm in sugarcane breeding. Investigations on cold tolerance in relation to different ploidies in S. spontaneum may promote the exploitation of its germplasm and accelerate the improvement of sugarcane varieties. Results A hypoploid clone 12–23 (2n = 54) and hyperploid clone 15–28 (2n = 92) of S. spontaneum were analysed under cold stress from morphological, physiological, and transcriptomic perspectives. Compared with clone 15–28, clone 12–23 plants had lower plant height, leaf length, internode length, stem diameter, and leaf width; depressed stomata and prominent bristles and papillae; and thick leaves with higher bulliform cell groups and thicker adaxial epidermis. Compared with clone 15–28, clone 12–23 showed significantly lower electrical conductivity, significantly higher water content, soluble protein content, and superoxide dismutase activity, and significantly higher soluble sugar content and peroxidase activity. Under cold stress, the number of upregulated genes and downregulated genes of clone 12–23 was higher than clone 15–28, and many stress response genes and pathways were affected and enriched to varying degrees, particularly sugar and starch metabolic pathways and plant hormone signalling pathways. Under cold stress, the activity of 6-phosphate glucose trehalose synthase, trehalose phosphate phosphatase, and brassinosteroid-signalling kinase and the content of trehalose and brassinosteroids of clone 12–23 increased. Conclusions Compared with hyperploid clone 15–28, hypoploid clone 12–23 maintained a more robust osmotic adjustment system through sugar accumulation and hormonal regulation, which resulted in stronger cold tolerance.
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- 2020
19. The timeline and risk factors of clinical progression of COVID-19 in Shenzhen, China
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Mengyuan Qu, Lei Liu, Xuan Zhou, Ruikun Chen, Fang Wang, Zhiyu Li, Qing He, Wenjie Xian, Kai Zhao, Xuan Li, Changxiang Lai, and Qiyuan Tang
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Male ,Time Factors ,Clinical progression ,lcsh:Medicine ,030204 cardiovascular system & hematology ,Logistic regression ,0302 clinical medicine ,Risk Factors ,Epidemiology ,Early prediction ,030212 general & internal medicine ,Stage (cooking) ,Child ,Aged, 80 and over ,General Medicine ,Middle Aged ,Hospitalization ,Treatment Outcome ,Child, Preschool ,Disease Progression ,Female ,Coronavirus Infections ,Adult ,medicine.medical_specialty ,China ,Coronavirus disease 2019 (COVID-19) ,Adolescent ,Retrospective analysis ,Pneumonia, Viral ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Betacoronavirus ,Young Adult ,Age Distribution ,Internal medicine ,medicine ,Humans ,Pandemics ,Risk factor ,COVID-19 ,Pneumonia ,Aged ,business.industry ,SARS-CoV-2 ,Research ,lcsh:R ,Disease progression ,Infant ,Retrospective cohort study ,Logistic Models ,ROC Curve ,business - Abstract
Background The novel coronavirus disease 2019(COVID-19) broke out globally. Early prediction of the clinical progression was essential but still unclear. We aimed to evaluate the timeline of COVID-19 development and analyze risk factors of disease progression.Methods In this retrospective study, we included 333 patients with laboratory-confirmed COVID-19 infection hospitalized in the Third People's Hospital of Shenzhen from 10 January to 10 February 2020. Epidemiological feature, clinical records, laboratory and radiology manifestations were collected and analyzed. 323 patients with mild-moderate symptoms on admission were observed to determine whether they exacerbated to severe-critically ill conditions (progressive group) or not (stable group). We used logistic regression to identify the risk factors associated with clinical progression.Results Of all the 333 patients, 70(21.0%) patients progressed into severe-critically ill conditions during hospitalization and assigned to the progressive group, 253(76.0%) patients belonged to the stable group, another 10 patients were severe before admission. we found that the clinical features of aged over 40 (3.80[1.72, 8.52]), males (2.21[1.20, 4.07]), with comorbidities (1.78[1.13, 2.81]) certain exposure history (0.38[0.20, 0.71]), abnormal radiology manifestations (3.56[1.13, 11.40]), low level of T lymphocytes (0.99[0.997, 0.999]), high level of NLR (0.99[0.97, 1.01]), IL-6 (1.05[1.03, 1.07]) and CRP (1.67[1.12, 2.47]) were the risk factors of disease progression by logistic regression.Conclusions the potential risk factors of males, older age, with comorbidities, low T lymphocyte level and high level of NLR, CRP, IL-6 can help to predict clinical progression of COVID-19 at an early stage.
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- 2020
20. Age-period-cohort analysis of kidney cancer deaths attributable to high body-mass index in China and U.S. adults
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Runtang Meng, Sumaira Mubarik, Yong Yu, Chuanhua Yu, Fang Wang, Yafeng Wang, Xiaoxue Liu, and Minsheng Wang
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Adult ,Male ,medicine.medical_specialty ,China ,Age-period-cohort effect ,High BMI ,Comorbidity ,Risk Assessment ,Body Mass Index ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Epidemiology ,Cohort Effect ,medicine ,Trend ,Humans ,030212 general & internal medicine ,Obesity ,Mortality ,Aged ,Aged, 80 and over ,business.industry ,lcsh:Public aspects of medicine ,Mortality rate ,Public health ,Public Health, Environmental and Occupational Health ,lcsh:RA1-1270 ,Kidney cancer ,Middle Aged ,medicine.disease ,Kidney Neoplasms ,Cohort effect ,Social Perception ,030220 oncology & carcinogenesis ,Cohort ,Female ,Biostatistics ,business ,Risk assessment ,Demography ,Research Article - Abstract
Background Statistical data on burden of kidney cancer and the relavant risk factors are valuable for policy-making. This study aims to estimate kidney cancer deaths and high body-mass index (BMI) attributable to the deaths by gender and age group in China adults, compared with U.S. Methods We extracted kidney cancer data (1990–2017) about the age-standardized rates using the comparative risk assessment framework of the 2017 Global Burden of Disease study. We performed an age-period-cohort (APC) analysis to estimate trends of kidney cancer mortality attributable to high BMI. Results During 1990–2017, age-standardized mortality rate of kidney cancer was increasing in China but decreasing in U.S. The mortality attributable to high BMI in China showed a general increasing trend, while that in U.S. men was increasing and tended to be stable in women since 1995. APC analysis showed a similar pattern of age effect between China and U.S. adults, which substantially increased from 20 to 24 to 90–94 age group. Differently, the period effect rapidly increased in China than U.S. adults during 1990–2017. The cohort effect peaked in the earlier cohort born in 1902–1906 in China, and it declined consistently in U.S. with exception of 1902–1906 and 1907–1911 birth cohort. Conclusions The kidney cancer deaths attributable to high BMI, and period effect have been generally increasing in China adults, compared with U.S. adults in which the trend tends to be stable in recent years. The rapid aging may also intensify the increasing trend of kidney cancer death in China. Effective measures should be conducted on body weight control and care for kidney cancer prevention.
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- 2020
21. Ovarian hyperstimulation syndrome in a frozen-thawed embryo transfer pregnancy: a rare case report
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Rong Wang, Feifeng Wang, Fang Wang, Lihua Yang, and Libo Zou
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Adult ,medicine.medical_specialty ,Pregnancy Rate ,medicine.medical_treatment ,Ovarian hyperstimulation syndrome ,Single Embryo Transfer ,Case Report ,Controlled ovarian hyperstimulation ,Fertilization in Vitro ,lcsh:Gynecology and obstetrics ,Chorionic Gonadotropin ,Human chorionic gonadotropin ,03 medical and health sciences ,0302 clinical medicine ,Ovulation Induction ,Pregnancy ,medicine ,Frozen-thawed embryo transfer ,Humans ,Dichorionic diamniotic triplet pregnancy ,lcsh:RG1-991 ,Gynecology ,Cryopreservation ,030219 obstetrics & reproductive medicine ,In vitro fertilisation ,business.industry ,Obstetrics and Gynecology ,medicine.disease ,Embryo Transfer ,Pregnancy, Triplet ,Embryo transfer ,Multifetal pregnancy reduction ,030220 oncology & carcinogenesis ,embryonic structures ,Ovulation induction ,Female ,business ,Live Birth - Abstract
Background Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic complication of ovarian stimulation. Prevention and early recognition of OHSS are important to ensure patient safety. Case presentation In this case, we reported a patient who underwent controlled ovarian hyperstimulation (COH) and in vitro fertilization (IVF). All embryos were cryopreserved to reduce possible OHSS. However, OHSS still occurred after the patient had a frozen-thawed embryo transfer (FET) with hormone replacement therapy (HRT) and obtained a dichorionic diamniotic triplet pregnancy. After multifetal pregnancy reduction (MFPR) and supportive treatment, all the symptoms regressed. Conclusions Prompt recognition of OHSS, especially in patients who have no history of ovulation induction and fresh embryo transfer, is very important. Multiple pregnancies may lead to severe OHSS because of the high level of human chorionic gonadotropin (hCG) in the early stages. We suggest that a single embryo transfer may be necessary and beneficial for patients.
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- 2020
22. Gender-specific prevalence and associated factors of major depressive disorder and generalized anxiety disorder in a Chinese rural population: the Henan rural cohort study
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Dou Qiao, Yan Wang, Zhicheng Luo, Fang Wang, Xue Liu, Xiaotian Liu, Xiaokang Dong, Yitan Hou, Yuqian Li, Jingjing Jiang, Ruiying Li, and Chongjian Wang
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Adult ,Male ,Rural Population ,medicine.medical_specialty ,China ,Generalized anxiety disorder ,Adolescent ,Poison control ,Major depressive disorder ,Cohort Studies ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Risk Factors ,Epidemiology ,mental disorders ,medicine ,Prevalence ,Humans ,030212 general & internal medicine ,Sex Distribution ,Aged ,Depressive Disorder, Major ,business.industry ,lcsh:Public aspects of medicine ,Associated factors ,Public Health, Environmental and Occupational Health ,lcsh:RA1-1270 ,Middle Aged ,medicine.disease ,Comorbidity ,Anxiety Disorders ,Marital status ,Female ,business ,Body mass index ,030217 neurology & neurosurgery ,Demography ,Cohort study ,Research Article - Abstract
Background This study aims to investigate the prevalence and associated factors of major depressive disorder (MDD) and generalized anxiety disorder (GAD) by gender in Chinese rural adults. Methods A total of 29,993 participants aged from 18 to 79 years from the Henan Rural Cohort Study were included in this study. The Patient Health Questionnaire-2 (PHQ-2) and Generalized Anxiety Disorder-2 (GAD-2) were used to assess MDD and GAD through a face-to-face interview. Multivariate logistic regression model was conducted to analyze the associated factors for MDD and GAD. Results The age-standardized prevalence of MDD and GAD (and 95%CI) in the total sample were 5.41% (5.17–5.66%) and 4.94% (4.71–5.18%), respectively. Besides, the crude prevalence in women were significantly higher than men for both MDD (6.81% vs. 4.77%) and GAD (6.63% vs. 3.93%) (both P r = 0.88, P = 0.01). Further analysis revealed that age, sex, marital status, educational level, per capita monthly income, drinking, physical activity, and body mass index were associated with MDD and GAD in the overall sample. Gender difference was found among age groups for MDD (Pinteraction Conclusions These findings showed that Chinese rural adults were at low risk for prevalence of MDD and GAD. Women had higher prevalence and risks for MDD and GAD compared with men, indicating that women deserved more attention. Gender-specific interventions on the modifiable associated factors are urgently needed to improve the mental conditions for Chinese rural population. Clinical trial registration The Henan Rural Cohort Study has been registered in the Chinese Clinical Trial Register (Registration number: ChiCTR-OOC-15006699). Date of registration: 2015-07-06.
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- 2019
23. Chemotherapeutic drugs stimulate the release and recycling of extracellular vesicles to assist cancer cells in developing an urgent chemoresistance
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Meng Xu, Dongjuan Qiao, Zhen Chen, Jiye Cai, Likun Chen, Xiaokun Wang, Shupeng Chen, Liwu Fu, Fang Wang, and Liyan Huang
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0301 basic medicine ,Cancer Research ,Lung Neoplasms ,Intercellular transfer ,Gene Expression ,Antineoplastic Agents ,Biology ,Multidrug resistance ,lcsh:RC254-282 ,Models, Biological ,Flow cytometry ,Immunophenotyping ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Secretion ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Cytotoxicity ,medicine.diagnostic_test ,Dose-Response Relationship, Drug ,Research ,Cancer ,ABCB1 ,Cell sorting ,Extracellular vesicles ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Multiple drug resistance ,Disease Models, Animal ,030104 developmental biology ,Oncology ,Cell culture ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Molecular Medicine ,Chemotherapeutic drugs - Abstract
Background Chemotherapy is a widely used treatment for cancer. However, the development of acquired multidrug resistance (MDR) is a serious issue. Emerging evidence has shown that the extracellular vesicles (EVs) mediate MDR, but the underlying mechanism remains unclear, especially the effects of chemotherapeutic agents on this process. Methods Extracellular vesicles isolation was performed by differential centrifugation. The recipient cells that acquired ATP-binding cassette sub-family B member 1 (ABCB1) proteins were sorted out from co-cultures according to a stringent multi-parameter gating strategy by fluorescence-activated cell sorting (FACS). The transfer rate of ABCB1 was measured by flow cytometry. The xenograft tumor models in mice were established to evaluate the transfer of ABCB1 in vivo. Gene expression was detected by real-time PCR and Western blotting. Results Herein, we show that a transient exposure to chemotherapeutic agents can strikingly increase Rab8B-mediated release of extracellular vesicles (EVs) containing ABCB1 from drug-resistant cells, and accelerate these EVs to circulate back onto plasma membrane of sensitive tumor cells via the down-regulation of Rab5. Therefore, intercellular ABCB1 transfer is significantly enhanced; sensitive recipient cells acquire a rapid but unsustainable resistance to evade the cytotoxicity of chemotherapeutic agents. More fascinatingly, in the xenograft tumor models, chemotherapeutical drugs also locally or distantly increase the transfer of ABCB1 molecules. Furthermore, some Non-small-cell lung carcinoma (NSCLC) patients who are undergoing primary chemotherapy have a rapid increase of ABCB1 protein in their monocytes, and this is obviously associated with poor chemotherapeutic efficacy. Conclusions Chemotherapeutic agents stimulate the secretion and recycling of ABCB1-enriched EVs through the dysregulation of Rab8B and Rab5, leading to a significant increase of ABCB1 intercellular transfer, thus assisting sensitive cancer cells to develop an urgent resistant phenotype. Our findings provide a new molecular mechanism of how chemotherapeutic drugs assist sensitive cancer cells in acquiring an urgent resistance.
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- 2019
24. Clinical significance of the histological and molecular characteristics of ependymal tumors: a single institution case series from China
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Jing Zeng, Wan-Ming Hu, Shaoyan Xi, Yinsheng Chen, Zhongping Chen, Fang Wang, Ke Sai, and Jing Wang
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0301 basic medicine ,Male ,Cancer Research ,Pathology ,Necrosis ,H3K27me3 ,Kaplan-Meier Estimate ,Histones ,0302 clinical medicine ,Surgical oncology ,Atypia ,Cyclin D1 ,Spinal Cord Neoplasms ,Child ,In Situ Hybridization, Fluorescence ,medicine.diagnostic_test ,Brain Neoplasms ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Ependymal tumor ,Prognosis ,Immunohistochemistry ,Oncology ,Ependymoma ,030220 oncology & carcinogenesis ,Female ,medicine.symptom ,Research Article ,Adult ,medicine.medical_specialty ,China ,Adolescent ,RELA ,lcsh:RC254-282 ,03 medical and health sciences ,Young Adult ,Genetics ,medicine ,Biomarkers, Tumor ,Humans ,Clinical significance ,Pathological ,business.industry ,Transcription Factor RelA ,medicine.disease ,030104 developmental biology ,Ki-67 Antigen ,Histological characteristics ,Neoplasm Grading ,business ,Fluorescence in situ hybridization ,Follow-Up Studies - Abstract
Background Ependymal tumors are pathologically defined intrinsic neoplasms originating in the intracranial compartments or the spinal cord that affect both children and adults. The recently integrated classification of ependymomas based on both histological and molecular characteristics is capable of subgrouping patients with various prognoses. However, the application of histological and molecular markers in Chinese patients with ependymomas has rarely been reported. We aimed to demonstrate the significance of histological characteristics, the v-relavian reticuloendotheliosis viral oncogene homolog A (RELA) fusions and other molecular features in ependymal tumors. Methods We reviewed the histological characteristics of ependymal tumors using conventional pathological slides and investigate the RELA fusions and Cylclin D1 (CCND1) amplification by Fluorescence in situ hybridization (FISH) and trimethylation of histone 3 lysine 27 (H3K27me3) expression by immunohistochemistry (IHC) methods. SPSS software was used to analyze the data. Results We demonstrated that hypercellularity, atypia, microvascular proliferation, necrosis, mitosis, and an elevated Ki-67 index, were tightly associated with an advanced tumor grade. Tumor location, necrosis, mitosis and the Ki-67 index were related to the survival of the ependymomas, but Ki67 was the only independent prognostic factor. Additionally, RELA fusions, mostly presented in pediatric grade III intracranial ependymomas, indicated decreased survival times of patients, and closely related to the patients’ age, tumor grade, cellularity, cellular atypia, necrosis and Ki67 index in the intracranial ependymal tumors, whereas reduction of H3K27me3 predicted the worse prognosis in ependymal tumors. Conclusions Histological and molecular features facilitate tumor grading and prognostic predictions for ependymal tumors in Chinese patients.
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- 2019
25. Grey matter abnormalities in Tourette syndrome: an activation likelihood estimation meta-analysis.
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Fang Wen, Junjuan Yan, Liping Yu, Fang Wang, Jingran Liu, Ying Li, and Yonghua Cui
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Background: Tourette syndrome (TS) is a neurodevelopmental disorder defined by the continual presence of primary motor and vocal tics. Grey matter abnormalities have been identified in numerous studies of TS, but conflicting results have been reported. This study was an unbiased statistical meta-analysis of published neuroimaging studies of TS structures. Methods: A voxel quantitative meta-analysis technique called activation likelihood estimation (ALE) was used. The meta-analysis included six neuroimaging studies involving 247 TS patients and 236 healthy controls. A statistical threshold of p < 0.05 was established based on the false discovery rate and a cluster extent threshold of 50 voxels. Results: We found that grey matter volumes were significantly increased in the bilateral thalamus, right hypothalamus, right precentral gyrus, left postcentral gyrus, left inferior parietal lobule, right lentiform nucleus, and left insula of TS patients compared to those of healthy controls. In contrast, grey matter volumes were significantly decreased in the bilateral postcentral gyrus, bilateral anterior cingulate, bilateral insula, left posterior cingulate and left postcentral gyrus of TS patients compared to those of healthy controls. Conclusions: Our present meta-analysis primarily revealed significant increases in grey matter volumes in the thalamus and lentiform nucleus, and decreased grey matter volumes in the anterior cingulate gyrus, of TS patients compared to those in healthy controls. Most of these identified regions are associated with cortico-striato-thalamocortical circuits. Further studies with larger sample sizes are needed to confirm these changes in grey matter volumes in TS patients. [ABSTRACT FROM AUTHOR]
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- 2021
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26. Macular vascular changes in pregnant women with gestational diabetes mellitus by optical coherence tomography angiography.
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Guodong Liu, Fang Wang, Liu, Guodong, and Wang, Fang
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Background: Retinal capillary is vulnerable to diabetes, whether gestational diabetes mellitus (GDM) eyes without clinical retinopathy have capillary abnormalities has not been well studied. To observe the microvasculature changes in eyes of GDM women compared with normoglycemic pregnant women and non-pregnant women by optical coherence tomography-angiography (OCT-A).Methods: GDM women, age-matched normoglycemic pregnant women and non-pregnant women were included in this study. All subjects were examined by OCT-A, vascular density and macular foveal parameters were measured automatically.Results: Thirty eight non-pregnant women (NC group), thirty pregnant women without GDM (PC group), and thirty one GDM women (GDM group) were included in this study. There was a significant reduction of vascular density in superficial capillary layer, but an increase in deep capillary layer in PC and GDM groups (P < 0.001). When in terms of the average vascular density, the difference was insignificant among these three groups. Although all the measurements were similar between PC and GDM groups, more capillary "dropout" changes were detected in GDM group. Unexpectedly, the abnormal changes of central macular thickness thinning and foveal avascular zone enlargement seen during pregnancy were improved when compared to PC group.Conclusions: The changes of vascular density implied the redistribution of capillary network from superficial to deep layer under pregnancy and GDM states. Although the transient hyperglycemia aggravates the changes of capillary "dropout", GDM group revealed the improvement of central macular thickness thinning and foveal avascular zone enlargement during pregnancy. [ABSTRACT FROM AUTHOR]- Published
- 2021
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27. Correction to: Adiponectin aggravates bone erosion by promoting osteopontin production in synovial tissue of rheumatoid arthritis
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Jie Qian, Rui Liu, Fang Wang, Miaojia Zhang, Pengfei Zhao, Qian Zhang, Yani Wang, Xiaoxuan Sun, Lingxiao Xu, Wenfeng Tan, Nan Che, Qin Wu, and Wenhua Xuan
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0301 basic medicine ,Male ,medicine.medical_specialty ,Pathology ,lcsh:Diseases of the musculoskeletal system ,Gene Expression ,Bone erosion ,Arthritis, Rheumatoid ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Cell Movement ,Internal medicine ,medicine ,Animals ,Humans ,Osteopontin ,Synovial tissue ,Cells, Cultured ,030203 arthritis & rheumatology ,Adiponectin ,biology ,business.industry ,Macrophages ,Synovial Membrane ,Correction ,Fibroblasts ,medicine.disease ,Rheumatology ,030104 developmental biology ,RAW 264.7 Cells ,Mice, Inbred DBA ,Rheumatoid arthritis ,biology.protein ,RNA Interference ,lcsh:RC925-935 ,business - Abstract
We have previously reported that adiponectin (AD), an adipokine that is secreted by adipocytes, correlates well with progressive bone erosion in rheumatoid arthritis (RA). The exact mechanism of AD in promoting joint destruction remains unclear. Osteopontin (OPN) is required for osteoclast recruitment. We hypothesized that AD exacerbates bone erosion by inducing OPN expression in synovial tissue. This study aimed to evaluate a novel role for AD in RA.The serum levels of AD and OPN were determined in 38 patients with RA, 40 patients with osteoarthritis (OA), and 20 healthy controls using enzyme-linked immunosorbent assay (ELISA). AD and OPN production were measured by double immunofluorescence in RA and OA synovial tissue. Quantitative real-time PCR and immunofluorescence were used to evaluate the mRNA and protein expression levels of OPN in RA synovial fibroblasts (RASFs) and OA synovial fibroblasts after pre-incubation with AD, respectively. Migration of the RAW264.7 osteoclast precursor cell line was assessed using the Transwell migration assay and co-culture system. Bone destruction and osteoclastogenesis were assessed by immunohistochemical staining, microcomputed tomography and tartrate-resistant acid phosphatase (TRAP) staining in AD-treated collagen-induced arthritis (CIA) mice with or without OPN silencing. The expression levels of OPN and integrin αOur results indicated that the AD and OPN expression levels increased noticeably and were associated with each other in the RA serum. The AD distribution was coincident with that of OPN in the RA synovial tissue. AD stimulation of RASFs increased OPN production in a dose-dependent manner. AD-treated RASFs promoted RAW264.7 cell migration, and the effect was blocked with a specific antibody against OPN. Silencing of OPN using lentiviral-OPN short hairpin RNA reduced the number of TRAP-positive osteoclasts and the extent of bone erosion in the AD-treated CIA mice. When bound to integrin αOur study provides new evidence of AD involvement in bone erosion. AD induces the expression of OPN, which recruits osteoclasts and initiates bone erosion. These data highlight AD as a novel target for RA treatment.
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- 2018
28. SALL1 functions as a tumor suppressor in breast cancer by regulating cancer cell senescence and metastasis through the NuRD complex
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Chunling Ma, Guangyong Peng, Michael Rauchman, Xiaoli Zhong, Yanping Zhang, Fusheng Si, Eddy C. Hsueh, Fang Wang, Mark A. Varvares, Susan M. Kiefer, Jian Ye, Bing Han, Lynn Robbins, and Pamela Hunborg
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0301 basic medicine ,Cancer Research ,Tumor suppressor gene ,Estrogen receptor ,Down-Regulation ,Triple Negative Breast Neoplasms ,Biology ,NuRD complex ,medicine.disease_cause ,Senescence ,lcsh:RC254-282 ,Metastasis ,03 medical and health sciences ,Mice ,Breast cancer ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Neoplasm Metastasis ,skin and connective tissue diseases ,Triple-negative breast cancer ,Cellular Senescence ,Cell Proliferation ,SALL1 ,Research ,Cancer ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Coculture Techniques ,3. Good health ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Oncology ,Cancer cell ,Tumorigenesis ,Cancer research ,MCF-7 Cells ,Molecular Medicine ,Female ,Carcinogenesis ,Neoplasm Transplantation ,Mi-2 Nucleosome Remodeling and Deacetylase Complex ,Transcription Factors - Abstract
Background SALL1 is a multi-zinc finger transcription factor that regulates organogenesis and stem cell development, but the role of SALL1 in tumor biology and tumorigenesis remains largely unknown. Methods We analyzed SALL1 expression levels in human and murine breast cancer cells as well as cancer tissues from different types of breast cancer patients. Using both in vitro co-culture system and in vivo breast tumor models, we investigated how SALL1 expression in breast cancer cells affects tumor cell growth and proliferation, metastasis, and cell fate. Using the gain-of function and loss-of-function strategies, we dissected the molecular mechanism responsible for SALL1 tumor suppressor functions. Results We demonstrated that SALL1 functions as a tumor suppressor in breast cancer, which is significantly down-regulated in the basal like breast cancer and in estrogen receptor (ER), progesterone receptor (PR) and epidermal growth factor receptor 2 (HER2) triple negative breast cancer patients. SALL1 expression in human and murine breast cancer cells inhibited cancer cell growth and proliferation, metastasis, and promoted cell cycle arrest. Knockdown of SALL1 in breast cancer cells promoted cancer cell growth, proliferation, and colony formation. Our studies revealed that tumor suppression was mediated by recruitment of the Nucleosome Remodeling and Deacetylase (NuRD) complex by SALL1, which promoted cancer cell senescence. We further demonstrated that the mechanism of inhibition of breast cancer cell growth and invasion by SALL1-NuRD depends on the p38 MAPK, ERK1/2, and mTOR signaling pathways. Conclusion Our studies indicate that the developmental control gene SALL1 plays a critical role in tumor suppression by recruiting the NuRD complex and thereby inducing cell senescence in breast cancer cells.
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- 2018
29. Genetic analysis of Wnt/PCP genes in neural tube defects
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Yunping Lei, Xuanye Cao, Yufang Zheng, Yihua Bao, Hongyan Wang, Zhongzhong Chen, Ting Zhang, Fang Wang, Richard H. Finnell, and Rui Peng
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0301 basic medicine ,lcsh:Internal medicine ,congenital, hereditary, and neonatal diseases and abnormalities ,lcsh:QH426-470 ,Biology ,Compound heterozygosity ,Genetic analysis ,03 medical and health sciences ,Wnt ,0302 clinical medicine ,Genetics ,medicine ,Variant ,lcsh:RC31-1245 ,Gene ,Genetics (clinical) ,Loss function ,Neural tube defects ,CELSR ,Wnt signaling pathway ,Neural tube ,Heterozygote advantage ,Phenotype ,lcsh:Genetics ,030104 developmental biology ,medicine.anatomical_structure ,PCP (planar cell polarity) ,030217 neurology & neurosurgery ,Research Article - Abstract
Background Mouse homozygous mutants in Wnt/planar cell polarity (PCP) pathway genes have been shown to cause neural tube defects (NTDs) through the disruption of normal morphogenetic processes critical to neural tube closure (NTC). Knockout mice that are heterozygotes of single PCP genes likely fail to produce NTD phenotypes, yet damaging variants detected in human NTDs are almost always heterozygous, suggesting that other deleterious interacting variants are likely to be present. Nonetheless, the Wnt/PCP pathway remains a genetic hotspot. Addressing these issues is essential for understanding the genetic etiology of human NTDs. Methods We performed targeted next-generation sequencing (NGS) on 30 NTD-predisposing Wnt/PCP pathway genes in 184 Chinese NTD cases. We subsequently replicated our findings for the CELSR1 gene in an independent cohort of 292 Caucasian NTD samples from the USA. Functional validations were confirmed using in vitro assays. Results CELSR1, CELSR2 and CELSR3 genes were significantly clustered with rare driver coding mutations (q-valueCELSR1 was significantly enriched in NTDs compared with the LoF counts in the ExAC database (p CELSR2 p.Thr2026Met and DVL3 p.Asp403Asn result in down regulation of PCP signals. Conclusions These data indicate rare damaging variants of the CELSR genes, identified in ~ 14% of NTD cases, are expected to be driver genes in the Wnt/PCP pathway. Compound damaging variants of CELSR genes and other Wnt/PCP genes, which were observed in 3.3% of the studied NTD cohort, are also expected to amplify these effects at the pathway level.
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- 2018
30. World Congress Integrative Medicine & Health 2017: part two
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Carolyn Ee, Sharmala Thuraisingam, Marie Pirotta, Simon French, Charlie Xue, Helena Teede, Agnete E. Kristoffersen, Fuschia Sirois, Trine Stub, Jennifer Engler, Stefanie Joos, Corina Güthlin, Jennifer Felenda, Christiane Beckmann, Florian Stintzing, Roni Evans, Gert Bronfort, Daniel Keefe, Anna Taberko, Linda Hanson, Alex Haley, Haiwei Ma, Joseph Jolton, Lana Yarosh, Francis Keefe, Jung Nam, Liwanag Ojala, Mary J. Kreitzer, Careen Fink, Karin Kraft, Andrew Flower, George Lewith, Kim Harman, Beth Stuart, Felicity L. Bishop, Jane Frawley, Lilla Füleki, Eva Kiss, Tamas Vancsik, Tibor Krenacs, Martha Funabashi, Katherine A. Pohlman, Silvano Mior, Haymo Thiel, Michael D. Hill, David J. Cassidy, Michael Westaway, Jerome Yager, Eric Hurwitz, Gregory N. Kawchuk, Maeve O’Beirne, Sunita Vohra, Isabelle Gaboury, Chantal Morin, Katharina Gaertner, Loredana Torchetti, Martin Frei-Erb, Michael Kundi, Michael Frass, Eugenia Gallo, Valentina Maggini, Mattia Comite, Francesco Sofi, Sonia Baccetti, Alfredo Vannacci, Mariella Di Stefano, Maria V. Monechi, Luigi Gori, Elio Rossi, Fabio Firenzuoli, Rocco D. Mediati, Giovanna Ballerini, Paula Gardiner, Anna S. Lestoquoy, Lily Negash, Sarah Stillman, Prachi Shah, Jane Liebschutz, Pamela Adelstein, Christine Farrell-Riley, Ivy Brackup, Brian Penti, Robert Saper, Isabel Giralt Sampedro, Gilda Carvajal, Andreas Gleiss, Marie M. Gross, Dorothea Brendlin, Jonas Röttger, Wiebke Stritter, Georg Seifert, Noelle Grzanna, Rainer Stange, Peter W. Guendling, Wen Gu, Yan Lu, Jie Wang, Chengcheng Zhang, Hua Bai, Yuxi He, Xiaoxu Zhang, Zhengju Zhang, Dali Wang, Fengxian Meng, Alexander Hagel, Heinz Albrecht, Claudia Vollbracht, Wolfgang Dauth, Wolfgang Hagel, Francesco Vitali, Ingo Ganzleben, Hans Schultis, Peter Konturek, Jürgen Stein, Markus Neurath, Martin Raithel, Bianka Krick, Heidemarie Haller, Petra Klose, Gustav Dobos, Sherko Kümmel, Holger Cramer, Felix J. Saha, Anna Kowoll, Barbara Ebner, Bettina Berger, Kyung-Eun Choi, Lisha He, Han Wang, X. He, C. Gu, Y. Zhang, Linhua Zhao, Xiaolin Tong, Xinhui He, Chengjuan Gu, Ying Zhang, Robin S. T. Ho, Vincent C. H. Chung, Xinyin Wu, Charlene H. L. Wong, Justin C. Y. Wu, Samuel Y. S. Wong, Alexander Y. L. Lau, Regina W. S. Sit, Wendy Wong, Michelle Holmes, Felicity Bishop, Lynn Calman, Dave Newell, Jonathan Field, Win L. Htut, Dongwoon Han, Da I. Choi, Soo J. Choi, Ha Y. Kim, Jung H. Hwang, Ching W. Huang, Bo H. Jang, Fang P. Chen, Seong G. Ko, Wenjing Huang, De Jin, Fengmei Lian, Soobin Jang, Kyeong H. Kim, Eun K. Lee, Seung H. Sun, Ho Y. Go, Youme Ko, Sunju Park, Yong C. Shin, Hubert Janik, Natalie Greiffenhagen, Jürgen Bolte, Mariusz Jaworski, Miroslawa Adamus, Aleksandra Dobrzynska, Michael Jeitler, Jessica Jaspers, Christel von Scheidt, Barbara Koch, Andreas Michalsen, Nico Steckhan, Christian Kessler, Wen-jing Huang, Bing Pang, Feng-Mei Lian, Miek Jong, Erik Baars, Anja Glockmann, Harald Hamre, Mosaburo Kainuma, Aya Murakami, Toshio Kubota, Daisuke Kobayashi, Yasuhiro Sumoto, Norihiro Furusyo, Shin-Ichi Ando, Takao Shimazoe, Olaf Kelber, S. Verjee, Eva Gorgus, Dieter Schrenk, Kathi Kemper, Ellie Hill, Nisha Rao, Gregg Gascon, John Mahan, Gunver Kienle, Jörg Dietrich, Claudia Schmoor, Roman Huber, Weon H. Kim, Mansoor Ahmed, Luzhu He, Jung Hye Hwang, Nora Meggyeshazi, Csaba Kovago, Anne K. Klaus, Roland Zerm, Danilo Pranga, Thomas Ostermann, Marcus Reif, Hans Broder von Laue, Benno Brinkhaus, Matthias Kröz, Daniela Rodrigues Recchia, Hans B. von Laue, Christien T. Klein-Laansma, Mats Jong, Cornelia von Hagens, Jean P. Jansen, Herman van Wietmarschen, Miek C. Jong, Seung-Ho Sun, Ho-Yeon Go, Chan-Yong Jeon, Yun-Kyung Song, Seong-Gyu Ko, Anna K. Koch, Sybille Rabsilber, Romy Lauche, Jost Langhorst, Milena Trifunovic-Koenig, Evi Koster, Diana Delnoij, Lena Kroll, Kathrin Weiss, Ai Kubo, Sarah Hendlish, Andrea Altschuler, Nancy Connolly, Andy Avins, Jon Wardle, David Lee, David Sibbritt, Jon Adams, Crystal Park, Gita Mishra, Johann Lechner, Inseon Lee, Younbyoung Chae, Jisu Lee, Seung H. Cho, Yujin Choi, Jee Y. Lee, Han S. Ryu, Sung S. Yoon, Hye K. Oh, Lyun K. Hyun, Jin O. Kim, Seong W. Yoon, Ju-Yeon Lee, Sang-Hoon Shin, Min Jang, Indra Müller, So-Hyun Janson Park, Lance Laird, Suzanne Mitchell, Xiaofei Li, Yunhui Wang, Jianhua Zhen, He Yu, Tiegang Liu, Xiaohong Gu, Hui Liu, Weiguo Ma, Xuezheng Shang, Yu Bai, Wei Liu, Collin Rooney, Amos Smith, Shirlene Lopes, Marcelo Demarzo, Maria do Patrocínio Nunes, Peter Lorenz, Carsten Gründemann, Miriam Heinrich, Manuel Garcia-Käufer, Franziska Grunewald, Silke Messerschmidt, Anja Herrick, Kim Gruber, Matthias Knödler, Carmen Steinborn, Taoying Lu, Lixin Wang, Darong Wu, Christina M Luberto, Daniel L. Hall, Emma Chad-Friedman, Suzanne Lechner, Elyse R. Park, Christina M. Luberto, Elyse Park, Janice Goodman, Sonja Luer, Matthias Heri, Klaus von Ammon, Ida Landini, Andrea Lapucci, Stefania Nobili, Enrico Mini, Clare McDermott, Selwyn Richards, Diane Cox, Sarah Frossell, Geraldine Leydon, Caroline Eyles, Hilly Raphael, Rachael Rogers, Michelle Selby, Charlotte Adler, Jo Allam, Xiangwei Bu, Honghong Zhang, Jianpeng Zhang, Michael Mikolasek, Jonas Berg, Claudia Witt, Jürgen Barth, Ivan Miskulin, Zdenka Lalic, Maja Miskulin, Albina Dumic, Damir Sebo, Aleksandar Vcev, Nasr A. A. Mohammed, Soo Jeung Choi, Hyea Bin Im, Anwesha Mukherjee, Amit Kandhare, Subhash Bodhankar, Prasad Thakurdesai, Niki Munk, Erica Evans, Amanda Froman, Matthew Kline, Matthew J. Bair, Frauke Musial, Terje Alræk, Harald J. Hamre, Lars Björkman, Vinjar M. Fønnebø, Feng-mei Lian, Qing Ni, Xiao-lin Tong, Xin-long Li, Wen-ke Liu, Shuo Feng, Xi-yan Zhao, Yu-jiao Zheng, Xue-min Zhao, Yi-qun Lin, Tian-yu Zhao, Xi-Yan Zhao, Hui Che Phd, Chen Zhang, Feng Liu, Lin-hua Zhao, Ru Ye, Cheng-juan Gu, Wenbo Peng, Diana De Carvalho, Mohamed El-Bayoumi, Bob Haig, Kimbalin Kelly, Darrell J. Wade, Emanuela Portalupi, Giampietro Gobo, Luigi Bellavita, Chiara Guglielmetti, Christa Raak, Myriam Teuber, Friedrich Molsberger, Ulrich von Rath, Ulrike Reichelt, Uta Schwanebeck, Sabine Zeil, Christian Vogelberg, Dolores Rodríguez Veintimilla, Guerrero Tapia Mery, Marisol Maldonado Villavicencio, Sandra Herrera Moran, Christian Sachse, Peter W Gündlin, Monirsadat Sahebkarkhorasani, Hoda Azizi, Dania Schumann, Tobias Sundberg, Matthew J. Leach, Susana Seca, Henry Greten, Sugir Selliah, Anu Shakya, Ha Yun Kim, Hyea B. Im, Anna Sherbakova, Gudrun Ulrich-Merzenich, Heba Abdel-Aziz, Erica Sibinga, Lindsey Webb, Jonathan Ellen, Kari Skrautvol, Dagfinn Nåden, Rhayun Song, Weronika Grabowska, Kamila Osypiuk, Gloria V. Diaz, Paolo Bonato, Moonkyoung Park, Jeffrey Hausdorff, Michael Fox, Lewis R. Sudarsky, Daniel Tarsy, James Novakowski, Eric A. Macklin, Peter M. Wayne, Inok Hwang, Sukhee Ahn, Myung-Ah Lee, Min K. Sohn, Oleg Sorokin, Dagmar Heydeck, Astrid Borchert, Christoph-Daniel Hohmann, Harmut Kühn, Clemens Kirschbaum, Tobias Stalder, Barbara Stöckigt, Michael Teut, Ralf Suhr, Daniela Sulmann, Chris Streeter, Patrica Gerbarg, Marisa Silveri, Richard Brown, John Jensen, Britta Rutert, Angelika Eggert, Alfred Längler, Christine Holmberg, Jin Sun, Xin Deng, Wen-Yuan Li, Bin Wen, Nicola Robinson, Jian-Ping Liu, Hyun K. Sung, Narae Yang, Seon M. Shin, Hee Jung, Young J. Kim, Woo S. Jung, Tae Y. Park, Kiyoshi Suzuki, Toshinori Ito, Seiya Uchida, Seika Kamohara, Naoya Ono, Mitsuyuki Takamura, Ayumu Yokochi, Kazuo Maruyama, Patricio Tapia, Katarzyna Thabaut, Anja Thronicke, Megan Steele, Harald Matthes, Cornelia Herbstreit, Friedemann Schad, Jiaxing Tian, Libo Yang, Tian Tian, Hewei Zhang, Xia Tian, CongCong Wang, Qian Yun Chai, Lijuan Zhang, Ruyu Xia, Na Huang, Yutong Fei, Jianpin Liu, Natalie Trent, Mindy Miraglia, Jeffrey Dusek, Edi Pasalis, Sat B. Khalsa, Milena Trifunovic-König, Anna Koch, Lisa Uebelacker, Geoffrey Tremont, Lee Gillette, Gary Epstein-Lubow, David Strong, Ana Abrantes, Audrey Tyrka, Tanya Tran, Brandon Gaudiano, Ivan Miller, Gerhild Ullmann, Yuhua Li, Sujata Vaidya, Vinod Marathe, Ana C. Vale, Jacquelyne Motta, Fabíola Donadão, Angela C. Valente, Luana C. Carvalho Valente, Ricardo Ghelman, Dusan Vesovic, Dragan Jevdic, Aleksandar Jevdic, Katarina Jevdic, Mihael Djacic, Dragica Letic, Drago Bozic, Marija Markovic, Slobodan Dunjic, Gordana Ruscuklic, Dezire Baksa, Kenan Vrca, Ann Vincent, Dietlind Wahner-Roedler, Mary Whipple, Maria M. Vogelius, Iris Friesecke, Peter W. Gündling, Saswati Mahapatra, Rebecca Hynes, Kimberly Van Rooy, Sherry Looker, Aditya Ghosh, Brent Bauer, Susanne Cutshall, Harald Walach, Ana Borges Flores, Michael Ofner, Andreas Kastner, Gerhard Schwarzl, Hermann Schwameder, Nathalie Alexander, Gerda Strutzenberger, Xianwei Bu, Jianping Zhang, Shang Wang, Jinfeng Shi, Yu Hao, Jun Wu, Zeji Qiu, Yuh-Hai Wang, Chi-Jung Lou, Sam Watts, Peter Wayne, Gloria Vergara-Diaz, Brian Gow, Jose Miranda, Lewis Sudarsky, Eric Macklin, Kathrin Wode, Jenny Bergqvist, Britt-Marie Bernhardsson, Johanna Hök Nordberg, Lena Sharp, Roger Henriksson, Yeonju Woo, Min K. Hyun, Hao Wu, Tian-Fang Wang, Yan Zhao, Yu Wei, Lei Tian, Lei He, Xue Wang, Ruohan Wu, Mei Han, Patrina H. Y. Caldwell, Shigang Liu, Jing Zhang, Jianping Liu, Qianyun Chai, Zhongning Guo, Congcong Wang, Zhijun Liu, Xun Li, I. J. Yang, V. Ruberio Lincha, S. H. Ahn, D. U. Lee, H. M. Shin, Lu Yang, N. Yang, H. Sung, S. M. Shin, H. Y. Go, H. Jung, Y. Kim, T. Y. Park, Angela Yap, Yu H. Kwan, Chuen S. Tan, Syed Ibrahim, Seng B. Ang, Alfred Yayi, Jeong E. Yoo, Ho R. Yoo, Sae B. Jang, Hye L. Lee, Ala’a Youssef, Shahira Ezzat, Amira Abdel Motaal, Hesham El-Askary, Xiaotong Yu, Yashan Cui, Younghee Yun, Jin-Hyang Ahn, Bo-Hyung Jang, Kyu-Seok Kim, Inhwa Choi, Augustina Glinz, Fadime ten Brink, Arnd Büssing, Christoph Gutenbrunner, Bert Helbrecht, Tiesheng Fang, Fengxion Meng, Zhiming Shen, Ruixin Zhang, Fan Wu, Ming Li, Xinyun Xuan, Xueyong Shen, Ke Ren, Brian Berman, Zian Zheng, Yuxiang Wan, Xueyan Ma, Fei Dong, Suzie Zick, Richard Harris, Go E. Bae, Jung N. Kwon, Hye Y. Lee, Jong K. Nam, Sang D. Lee, Dong H. Lee, Ji Y. Han, Young J. Yun, Ji H. Lee, Hye L. Park, Seong H. Park, Chiara Bocci, Giovanni B. Ivaldi, Ilaria Vietti, Ilaria Meaglia, Marta Guffi, Rubina Ruggiero, Marita Gualea, Emanuela Longa, Massimo Bonucci, Sarah Croke, Lourdes Diaz Rodriguez, Juan C. Caracuel-Martínez, Manuel F. Fajardo-Rodríguez, Angélica Ariza-García, Francisca García-De la Fuente, Manuel Arroyo-Morales, Maria S. Estrems, Vicente G. Gómez, Mónica Valero Sabater, Rosaria Ferreri, Simonetta Bernardini, Roberto Pulcri, Franco Cracolici, Massimo Rinaldi, Claudio Porciani, Peter Fisher, John Hughes, Ariadna Mendoza, Hugh MacPherson, Jacqueline Filshie, Antonia Di Francesco, Alberto Bernardini, Monica Messe, Vincenzo Primitivo, Piera A. Iasella, Monica Taminato, Jaqueline Do Carmo Alcantara, Katia R. De Oliveira, Debora C. De Azevedo Rodrigues, Juliana R. Campana Mumme, Olga K. Matsumoto Sunakozawa, Vicente Odone Filho, Joshua Goldenberg, Andrew Day, Masa Sasagawa, Lesley Ward, Kieran Cooley, Thora Gunnarsdottir, Ingibjorg Hjaltadottir, Mahdie Hajimonfarednejad, Nicole Hannan, Rut Hellsing, Susanne Andermo, Maria Arman, Iris von Hörsten, Patricia Vásquez Torrielo, Carmen L. Andrade Vilaró, Francisco Cerda Cabrera, Henny Hui, Eric Ziea, Dora Tsui, Joyce Hsieh, Christine Lam, Edith Chan, Mark P. Jensen, Samuel L. Battalio, Joy Chan, Karlyn A. Edwards, Kevin J. Gertz, Melissa A. Day, Leslie H. Sherlin, Dawn M. Ehde, Bo-Hyoung Jang, Anja Börner, Jihong Lee, Boram Lee, Gyu T. Chang, Alejandra Menassa, Yoshiharu Motoo, Jürgen Müller, Sabine Rabini, Bettina Vinson, Martin Storr, Martin Niemeijer, Joop Hoekman, Wied Ruijssenaaars, Faith C. Njoku, Arne J. Norheim, Filiz Okumus, and Halime Oncu-Celik
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lcsh:Other systems of medicine ,lcsh:RZ201-999 ,Meeting Abstracts - Published
- 2017
31. Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression
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Wei-zhao Peng, Fang Zhao, Fang Wang, Ren Ma, Chao-feng Li, Qingxia Zhang, Jia Yu, and Shuang Si
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Cancer Research ,miR-181a ,Apoptosis ,Biology ,Transfection ,Stomach Neoplasms ,microRNA ,Gene expression ,Humans ,MEG3 ,Cell Proliferation ,Competing endogenous RNA ,Cell growth ,Research ,RNA ,Long non-coding RNA ,MicroRNAs ,Cell Transformation, Neoplastic ,Oncology ,Cancer research ,Disease Progression ,Ectopic expression ,RNA, Long Noncoding ,Gastric cancer - Abstract
Background Long noncoding RNAs (lncRNAs) have recently emerged as important regulators in governing fundamental biological processes, and many of which are likely to have functional roles in tumorigenesis. Maternally expressed gene 3 (MEG3) gene encodes a lncRNA whose expression is lost in an expanding list of primary human tumors and tumor cell lines, however its biological role and regulatory mechanism in gastric cancer (GC) development and progression are poorly defined. Methods Quantitative RT-PCR analysis was used to determine whether aberrant MEG3 expression was associated with GC patients pTNM stage and pM state. Furthermore, the effect of ectopic expression of MEG3 on cell proliferation, migration, invasion and cell apoptosis was assessed by using CCK-8, wound healing, transwell invasion assays and flow cytometric analysis, respectively, in GC cell lines HGC-27 and MGC-803. Moreover, the competing endogenous RNA (ceRNA) activity of MEG3 on miR-181a was investigated via luciferase reporter assay and immunoblot analysis. Results MEG3 is decreased in GC patients and cell lines, and its expression was associated with metastatic GC. Furthermore, ectopic expression of MEG3 in HGC-27 and MGC-803 cells inhibited cell proliferation, migration, invasion, and promoted cell apoptosis, which might be due to MEG3 sequestering oncogenic miR-181 s in GC cells. Furthermore, MEG3 could up-regulated Bcl-2 via its competing endogenous RNA (ceRNA) activity on miR-181a. Conclusions These findings suggest that lncRNA MEG3, a ceRNA of miR-181 s, could regulate gastric carcinogenesis and may serve as a potential target for antineoplastic therapies.
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- 2015
32. A coronary artery disease-associated SNP rs6903956 contributed to asymptomatic hyperuricemia susceptibility in Han Chinese
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Miaojia Zhang, Yujing Zhu, Fang Wang, Wenfeng Tan, Jianfen Meng, and Xinli Li
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musculoskeletal diseases ,Male ,medicine.medical_specialty ,Pathology ,Asymptomatic hyperuricemia ,China ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Coronary Artery Disease ,Hyperuricemia ,Asymptomatic ,Polymorphism, Single Nucleotide ,SNP rs6903956 ,Coronary artery disease ,chemistry.chemical_compound ,Endocrinology ,Asian People ,Gene Frequency ,Internal medicine ,medicine ,SNP ,Humans ,Genetic Predisposition to Disease ,cardiovascular diseases ,Allele frequency ,Genetic Association Studies ,Asymptomatic Diseases ,Biochemistry, medical ,business.industry ,Research ,Biochemistry (medical) ,Case-control study ,nutritional and metabolic diseases ,Membrane Proteins ,Middle Aged ,medicine.disease ,Uric Acid ,chemistry ,Case-Control Studies ,Cardiology ,Uric acid ,Female ,medicine.symptom ,business - Abstract
Background To investigate the association of a coronary artery disease (CAD) risk SNP rs6903956 with asymptomatic hyperuricemia (aHU) susceptibility in Han Chinese. Methods Two hundred and twenty one patients with aHU and 447 healthy controls were recruited for this study. SNP rs6903956 were genotyped using TaqMan probe. Results The overall genotype and allele frequency distribution of the rs6903956 showed significant difference between aHU cases and controls (p
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- 2015
33. Adiponectin aggravates bone erosion by promoting osteopontin production in synovial tissue of rheumatoid arthritis.
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Jie Qian, Lingxiao Xu, Xiaoxuan Sun, Yani Wang, Wenhua Xuan, Qian Zhang, Pengfei Zhao, Qin Wu, Rui Liu, Nan Che, Fang Wang, Wenfeng Tan, and Miaojia Zhang
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- 2018
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34. The application of wide-field laser ophthalmoscopy in fundus examination before myopic refractive surgery.
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Lin Liu, Fang Wang, Ding Xu, Chunlei Xie, Jun Zou, Liu, Lin, Wang, Fang, Xu, Ding, Xie, Chunlei, and Zou, Jun
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OPHTHALMOSCOPY ,EYE examination ,OPHTHALMIC surgery ,FEMTOSECOND lasers ,CONTACT lenses ,REFRACTIVE errors ,MYOPIA ,RESEARCH funding ,RETINA ,RETINAL diseases ,PREOPERATIVE period ,DISEASE complications - Abstract
Background: To evaluate wide-field laser ophthalmoscopy (Optomap 200Tx) for screening retinal lesions before myopic refractive surgery.Methods: Seventy-eight eyes of 78 consecutive refractive surgery candidates were included in this study. All subjects underwent Optomap 200Tx, mydriatic slit-lamp lens examination and the Goldmann three-mirror contact lens examination, which was considered as the reference method for determining retinal lesions.Results: Forty of 78 eyes had retinal lesions (51.28%) and three eyes had retinal breaks (3.85%), which were diagnosed by the Goldmann three-mirror contact lens examination. Compared to the Goldmann three-mirror contact lens examination, the detection rate with the Optomap 200Tx was 91.73%% for retinal lesions, while the detection rate of mydriatic slit-lamp lens exams was 81.20%. There were no statistically significant differences among the three methods used for the diagnoses of myopic conus, tessellation and retinal breaks(all p > 0.05). For peripheral retinal lesions, the detection rate of the Optomap 200Tx examinations were similar to the Goldmann three-mirror contact lens exams (all p > 0.05), but were higher than the results of slit-lamp lens examinations (all p < 0.05). Regarding the vitreoretinal adhesions, the Goldmann three-mirror contact lens examinations had higher detection rates than did the Optomap 200Tx examinations (p = 0.031).Conclusions: The Optomap 200Tx examinations is a convenient and feasible method to determine fundus pathological changes in myopic patients, especially for patients who can not endure pupil dilation. In order to avoid misdiagnosis of peripheral retinal lesions, Goldmann three-mirror contact lens examination is needed. [ABSTRACT FROM AUTHOR]- Published
- 2017
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35. The macrophage C-type lectin receptor CLEC5A (MDL-1) expression is associated with early plaque progression and promotes macrophage survival.
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Weixin Xiong, Haibo Wang, Lin Lu, Rui Xi, Fang Wang, Gang Gu, Rong Tao, Xiong, Weixin, Wang, Haibo, Lu, Lin, Xi, Rui, Wang, Fang, Gu, Gang, and Tao, Rong
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ATHEROSCLEROSIS ,MACROPHAGES ,LECTINS ,DISEASE progression ,POLYMERASE chain reaction ,CASPASES ,GENETICS ,PROTEIN metabolism ,ANIMAL experimentation ,APOPTOSIS ,CELL culture ,CELL physiology ,CELL receptors ,GENE expression ,MICE ,PROTEINS ,RESEARCH funding ,PHYSIOLOGY - Abstract
Background: Biomarkers of early plaque progression are still elusive. Myeloid DAP12-associating lectin-1 (MDL-1), also called CLEC5A, is a C-type lectin receptor implicated in the progression of multiple acute and chronic inflammatory diseases. However, the relationship between its level and atherosclerosis is unknown. In this study, we aimed to investigate the correlation between macrophage MDL-1 expression and early atherosclerosis progression.Methods: Immunofluorescence staining, real-time PCR and western blot were performed to analyze MDL-1 expression in aorta or mice macrophages. The role of MDL-1 in macrophage survival was further investigated by adenovirus infection and TUNEL assay.Results: Significant MDL-1 expression was found in advanced human and apoE-/- mice atherosclerotic plaques, especially in lesional macrophages. In the model of atherosclerosis regression, we found MDL-1 expression was highly downregulated in lesional macrophages from ldlr-/- mouse regressive plaques, coincident with a reduction in lesional macrophage content and marker of M1 proinflammatory macrophages. Furthermore, we found MDL-1 was significantly expressed in inflammatory M1 subtype polarized bone marrow-derived macrophages. In vitro experiments, the level of MDL-1 was remarkably elevated in macrophages treated with pathophysiological drivers of plaque progression, such as oxidized low-density lipoprotein (ox-LDL) and hypoxia. Mechanistically, we demonstrated that MDL-1 overexpression notably promoted macrophage survival and decreased cleaved caspase-3 expression under ox-LDL stimulation, which suggested that it could maintain lesional macrophage survival and cause its accumulation.Conclusions: This study firstly demonstrated that MDL-1 is mainly expressed in atherosclerotic lesional macrophages and increased macrophage MDL-1 expression is associated with early plaque progression and promotes macrophage survival. [ABSTRACT FROM AUTHOR]- Published
- 2017
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36. Usefulness of interferon-γ release assay for the diagnosis of sputum smear-negative pulmonary and extra-pulmonary TB in Zhejiang Province, China.
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Lei Ji, Yong-Liang Lou, Zhong-Xiu Wu, Jin-Qin Jiang, Xing-Li Fan, Li-Fang Wang, Xiao-Xiang Liu, Peng Du, Jie Yan, and Ai-Hua Sun
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TUBERCULOSIS diagnosis ,INTERFERON gamma release tests - Abstract
Background: Quick diagnosis of smear-negative pulmonary tuberculosis (TB) and extra-pulmonary TB are urgently needed in clinical diagnosis. Our research aims to investigate the usefulness of the interferon-γ release assay (IGRA) for the diagnosis of smear-negative pulmonary and extra-pulmonary TB. Methods: We performed TB antibody and TB-IGRA tests on 389 pulmonary TB patients (including 120 smear-positive pulmonary TB patients and 269 smear-negative pulmonary TB patients), 113 extra-pulmonary TB patients, 81 patients with other pulmonary diseases and 100 healthy controls. Blood samples for the TB-Ab test and the TB-IGRA were collected, processed, and interpreted according to the manufacturer's protocol. Results: The detection ratio of smear-positive pulmonary TB patients and smear-negative pulmonary TB patients were 90.8% (109 of 120) and 89.6% (241 of 269), respectively. There was no statistically significant difference of its performance between these two sample sets (P > 0.05). The detection ratio of positive TB patients and extra-pulmonary TB patients were 90.0% (350 of 389) and 87.6% (99 of 113), respectively, which was not significantly different (P > 0.05). Conclusions: In this work, the total detection ratio using TB-IGRA was 89.4%, therefore TB-IGRA has diagnostic values in smear-negative pulmonary TB and extra-pulmonary TB diagnosis. [ABSTRACT FROM AUTHOR]
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- 2017
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37. Evaluation of left atrial volume and function using single-beat real-time three-dimensional echocardiography in atrial fibrillation patients.
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Qian Zhang, Ju-fang Wang, Qing-qing Dong, Qing Yan, Xiang-hong Luo, Xue-ying Wu, Jian Liu, and Ya-ping Sun
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THREE-dimensional echocardiography ,ATRIAL fibrillation ,SIMPSON'S rule (Numerical analysis) ,BLAND-Altman plot ,HEART beat - Abstract
Background: This study was aimed to evaluate the feasibility and accuracy of real-time three-dimensional echocardiography (RT-3DE) measurement of left atrial (LA) volume and function in comparison with twodimensional echocardiography (2DE) measurements in atrial fibrillation (AF) patients. Methods: A total of 50 pairs of AF patients and healthy controls were enrolled in this study. Indexed LA enddiastole volume (ILAEDV) and indexed LA end-systolic volume (ILAESV), as well as LA function indices such as segmental LA ejection fraction (LAEF), were assessed using 2DE Simpson's method and the RT-3DE method. Results: The images showed that regional LA volume-time curves and LAEF were disordered in AF patients. ILAEDV and ILAESV were markedly increased and global LAEF was significantly decreased in AF patients compared with those in healthy controls (P < 0.01). No significant differences were found in ILAEDV, ILAESV, and LAEF levels as determined by the RT-3DE method or 2DE Simpson's method. Bland-Altman analysis showed that the two methods agreed well for measuring ILAEDV, ILAESV, and segmental LAEF. Conclusion: The RT-3DE method may be a feasible and accurate method for evaluating LA volume and function of AF patients in clinical practice. [ABSTRACT FROM AUTHOR]
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- 2017
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38. Evaluation of immune protection induced by DNA vaccines from Haemaphysalis longicornis paramyosin in rabbits.
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Tian-Tian Zhang, Jin-Cheng Zhang, Xue-Jiao Cui, Jing-Jing Zheng, Ru Li, Fang Wang, Jing-Ze Liu, and Yong-Hong Hu
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HAEMAPHYSALIS longicornis ,ECTOPARASITES ,PROTEINS in the body ,ANIMAL vaccination ,IMMUNE response ,LABORATORY rabbits - Abstract
Background: Haemaphysalis longicornis is a blood-sucking ectoparasite that can cause diseases by transmitting some pathogens to humans and animals. Paramyosin (Pmy) is an immunomodulatory protein, which plays an important role in immune reactions against parasites. In this study, we evaluated the immune protection elicited by recombinant plasmids encoding H. longicornis Pmy in rabbits. Results: Rabbits vaccinated with pcDNA3.1(+)-Pmy developed high level of IgG compared to control group, suggesting that humoral immune response was induced by vaccination. On the fourth day after fed on the rabbit, some female adults died and the mortality rate from pcDNA3.1(+)-Pmy group (27.31%) was significantly higher than that of the control group (P< 0.0001). Other female ticks were attached to the rabbits until detachment, and the average engorgement weight, oviposition of female adult from pcDNA3.1(+)-Pmy group were 109.61 ± 4.24 mg and 48.39 ± 4.06 mg, respectively, which correspondingly resulted in 36 and 39% reduction compared with that of the control group (P< 0.0001). In brief, vaccination with Pmy plasmid DNA provided an overall efficacy of 50% in immune protection of rabbits. Conclusions: This study suggested that Pmy DNA vaccine can induce effective humoral immune response and partially protected rabbit against H. longicornis infection. [ABSTRACT FROM AUTHOR]
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- 2017
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39. Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters.
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Wan-Chen Li, Chien-Hao Huang, Chia-Ling Chen, Yu-Chien Chuang, Shu-Yun Tung, and Ting-Fang Wang
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TRICHODERMA reesei ,NUCLEOTIDE sequencing ,POINT mutation (Biology) ,GENE clusters ,CENTROMERE - Abstract
Background: Trichoderma reesei (Ascomycota, Pezizomycotina) QM6a is a model fungus for a broad spectrum of physiological phenomena, including plant cell wall degradation, industrial production of enzymes, light responses, conidiation, sexual development, polyketide biosynthesis, and plant-fungal interactions. The genomes of QM6a and its high enzyme-producing mutants have been sequenced by second-generation-sequencing methods and are publicly available from the Joint Genome Institute. While these genome sequences have offered useful information for genomic and transcriptomic studies, their limitations and especially their short read lengths make them poorly suited for some particular biological problems, including assembly, genome-wide determination of chromosome architecture, and genetic modification or engineering. Results: We integrated Pacific Biosciences and Illumina sequencing platforms for the highest-quality genome assembly yet achieved, revealing seven telomere-to-telomere chromosomes (34,922,528 bp; 10877 genes) with 1630 newly predicted genes and >1.5 Mb of new sequences. Most new sequences are located on AT-rich blocks, including 7 centromeres, 14 subtelomeres, and 2329 interspersed AT-rich blocks. The seven QM6a centromeres separately consist of 24 conserved repeats and 37 putative centromere-encoded genes. These findings open up a new perspective for future centromere and chromosome architecture studies. Next, we demonstrate that sexual crossing readily induced cytosine-to-thymine point mutations on both tandem and unlinked duplicated sequences. We also show by bioinformatic analysis that T. reesei has evolved a robust repeat-induced point mutation (RIP) system to accumulate AT-rich sequences, with longer AT-rich blocks having more RIP mutations. The widespread distribution of AT-rich blocks correlates genome-wide partitions with gene clusters, explaining why clustering of genes has been reported to not influence gene expression in T. reesei. Conclusion: Compartmentation of ancestral gene clusters by AT-rich blocks might promote flexibilities that are evolutionarily advantageous in this fungus' soil habitats and other natural environments. Our analyses, together with the complete genome sequence, provide a better blueprint for biotechnological and industrial applications. [ABSTRACT FROM AUTHOR]
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- 2017
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40. Reduced Kidney Function, Albuminuria, and Risks for All-cause and Cardiovascular Mortality in China: A Population-based Cohort Study.
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Jinwei Wang, Fang Wang, Shiwei Liu, Maigeng Zhou, Luxia Zhang, Minghui Zhao, Wang, Jinwei, Wang, Fang, Liu, Shiwei, Zhou, Maigeng, Zhang, Luxia, and Zhao, Minghui
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KIDNEY diseases ,ALBUMINURIA ,KIDNEY failure ,CARDIOVASCULAR disease related mortality ,GLOMERULAR filtration rate ,CARDIOVASCULAR disease diagnosis ,KIDNEY physiology ,CARDIOVASCULAR diseases ,CAUSES of death ,CHRONIC kidney failure ,LONGITUDINAL method ,PUBLIC health surveillance ,CROSS-sectional method ,DIAGNOSIS - Abstract
Background: Previous studies have indicated that reduced kidney function and albuminuria are associated with increased risk of mortality and adverse cardiovascular outcomes, however, the evidence from the Asian population is limited. We investigated the association between the indicators of chronic kidney disease (CKD) and all-cause mortality, as well as cardiovascular mortality among a general population in China.Methods: We conducted an observational study among 47,204 Chinese adults, from a cross-sectional survey, whose survival status is identified through December 31, 2013. Estimated glomerular filtration rate (eGFR) and urinary albumin-to-creatinine ratio (ACR) were used as indicators of CKD. We determined the rates of all-cause and cardiovascular mortality.Results: The incidence rates for both all-cause and cardiovascular mortality increased with the advanced stages of reduced eGFR or elevated ACR. Elevated ACR levels were found to be significantly associated with increased risk of both all-cause and cardiovascular mortality, as shown in the Cox proportional hazards regression model. The multivariable adjusted hazard ratios (HR) associated with all-cause mortality were 1.26 (95% confidence interval [CI]: 1.04-1.53) for those with ACR 30-299 mg/g and 2.07 (95% CI: 1.40-3.04) with ACR ≥ 300 mg/g, compared to those with ACR <30 mg/g. The corresponding HRs for the above ACR levels associated with cardiovascular mortality were 1.08 (95% CI: 0.77-1.50) and 2.32 (95% CI: 1.31-4.12), respectively. We did not identify reduced eGFR as a risk predictor in the multivariable adjusted model for the adverse outcomes in the population, however, an interaction between eGFR and age were detected. Stratified analyses revealed that the associations of reduced eGFR (<60 mL/min/1.73 m2) with all-cause mortality were prominent among participants aged less than 65 years.Conclusions: Albuminuria was associated with an elevated risk of all-cause and cardiovascular mortality among the Chinese population, however, the association of reduced kidney function with all-cause mortality was not clear. [ABSTRACT FROM AUTHOR]- Published
- 2017
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41. Renal replacement therapy in acute kidney injury from a Chinese cross-sectional study: patient, clinical, socioeconomic and health service predictors of treatment.
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Fang Wang, Daqing Hong, Yafang Wang, Yunlin Feng, Li Wang, Li Yang, Wang, Fang, Hong, Daqing, Wang, Yafang, Feng, Yunlin, Wang, Li, Yang, Li, and ISN AKF 0 by 25 China Consortium
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KIDNEY diseases ,ACUTE kidney failure ,CRITICALLY ill ,HEMODIALYSIS ,PERITONEAL dialysis ,TREATMENT of acute kidney failure ,COMPARATIVE studies ,DEMOGRAPHY ,RESEARCH methodology ,MEDICAL care ,UTILIZATION review (Medical care) ,MEDICAL cooperation ,HEALTH outcome assessment ,PROGNOSIS ,RESEARCH ,RESEARCH evaluation ,RISK assessment ,SURVIVAL ,THERAPEUTICS ,SOCIOECONOMIC factors ,EVALUATION research ,DISEASE prevalence ,CROSS-sectional method ,DIAGNOSIS - Abstract
Background: Renal replacement therapy (RRT) is important to support critically ill patients with acute kidney injury (AKI). This study, a part of a nation-wide survey for AKI conducted by the ISN AKF 0 by 25 China Consortium, aims to study the current RRT practical situation and problems in China.Methods: The current study is a part of a nation-wide survey for AKI conducted by ISN AKF 0 by 25 China Consortium. The survey included 44 sites all over the country, including 22 academic hospitals in big cities and 22 local hospitals in smaller cities or rural areas. Of the 44 sites, all have access to PD and IHD, 93.5% are capable to perform CRRT. Of total 7604 AKI cases, 896 cases (11.8%) had indications for RRT and were included in the current abstract.Results: of the 896 patients that had indications for RRT, only 59.3% received RRT. Patients who were older, male, from lower income areas, in local hospitals, or with severe comorbidities, were less likely to receive RRT. RRT treatment was associated with lower mortality (OR = 0.58, 95%CI 0.38-0.89). The RRT modalities were continuous renal replacement therapy (CRRT) in 53.9%, intermittent hemodialysis (IHD) in 38.0%, CRRT complemented by IHD in 6.2%, CRRT complemented by peritoneal dialysis (PD) in 0.8% and PD in 1.1%. Of the subgroup of patients receiving RRT who did not have an indication for modality of CRRT, 36.8% in fact received CRRT, and their medical cost and mortality rate was higher (7944[4248, 16,055] vs. 5100[2948, 9396] US dollars, p < 0.001 and 10.6% vs. 4.4%, p = 0.047, respectively) compared with those treated with other RRT modalities).Conclusions: Extrapolated to the whole of China our results indicate that an estimated 139,000 patients with an indication of RRT are under treated without RRT over a year. Non-clinical factors influence RRT prescription for severe AKI patients. CRRT may be over-utilized in the treatment of severe AKI and the use of PD is extremely rare. These findings have implications for the effective application of medical resources in the treatment of severe AKI. [ABSTRACT FROM AUTHOR]- Published
- 2017
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42. The effects of family structure and function on mental health during China's transition: a cross-sectional analysis.
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Yao Cheng, Liuyi Zhang, Fang Wang, Ping Zhang, Beizhu Ye, and Yuan Liang
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SOCIAL change ,FAMILIES ,MENTAL health ,METROPOLITAN areas ,PROBABILITY theory ,QUESTIONNAIRES ,REGRESSION analysis ,RURAL conditions ,FAMILY relations ,CROSS-sectional method ,PSYCHOLOGY - Abstract
Background: Social change, intensified by industrialization and globalization, has not only changed people's work lives but also their personal lives, especially in developing countries. The aim of this study was to provide evidence and recommendations regarding family structure, function, and mental health to actively respond to rapid social change. Methods: A cross-sectional survey was conducted face-to-face and door-to-door from July 2011 to September 2012 in Hubei Province, central China. Family structure comprised alone, couple, nuclear family, and extended family; family function was measured using the family APGAR (Adaptation, Partnership, Growth, Affection, and Resolve) scale, and mental health was measured using the Chinese version of the 12-item General Health Questionnaire (GHQ-12). Results: The urban-vs-rural difference of family structure among alone, couple, nuclear family, and extended family was statistically significant (5.21% vs 4.62%; 27.36% vs 13.14%; 33.22% vs 27.74%; 34.20% vs 54.50%, respectively; p < 0.0001); and those difference of family function was not statistically significant (8.11 ± 2.13 vs 8.09 ± 2.27, p = 0.9372). The general linear regression showed that the effect of family structure on mental health, whether urban or rural, was not significant, however, the effect of family function was significant, especially regarding better family functioning with better mental health. Conclusions: Combined the effects of family structure and function on mental health, the external form of family (family structure) may not be important; while the internal quality of role (family function) might be key. Improving the residents' family function would be a priority strategy for family practice with their mental health. [ABSTRACT FROM AUTHOR]
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- 2017
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43. Identification and profiling of Cyprinus carpio microRNAs during ovary differentiation by deep sequencing.
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Fang Wang, Yongfang Jia, Po Wang, Qianwen Yang, QiYan Du, and ZhongJie Chang
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CARP , *GENE expression in fishes , *MICRORNA , *OVARIES , *OVUM , *TRANSFORMING growth factors , *PROTEIN kinases - Abstract
Background: MicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate gene expression by targeting specific mRNAs. However, the possible role of miRNAs in the ovary differentiation and development of fish is not well understood. In this study, we examined the expression profiles and differential expression of miRNAs during three key stages of ovarian development and different developmental stages in common carp Cyprinus carpio. Results: A total of 8765 miRNAs were identified, including 2155 conserved miRNAs highly conserved among various species, 145 miRNAs registered in miRBase for common carp, and 6505 novel miRNAs identified in common carp for the first time. Comparison of miRNA expression profiles among the five libraries identified 714 co-expressed and 2382 specific expressed miRNAs. Overall, 150, 628, and 431 specifically expressed miRNAs were identified in primordial gonad, juvenile ovary, and adult ovary, respectively. MiR-6758-3p, miR-3050-5p, and miR-2985-3p were highly expressed in primordial gonad, miR-3544-5p, miR-6877-3p, and miR-9086-5p were highly expressed in juvenile ovary, and miR-154-3p, miR-5307-5p, and miR-3958-3p were highly expressed in adult ovary. Predicted target genes of specific miRNAs in primordial gonad were involved in many reproductive biology signaling pathways, including transforming growth factor-β, Wnt, oocyte meiosis, mitogen-activated protein kinase, Notch, p53, and gonadotropin-releasing hormone pathways. Target-gene prediction revealed upward trends in miRNAs targeting male-bias genes, including dmrt1, atm, gsdf, and sox9, and downward trends in miRNAs targeting femalebias genes including foxl2, smad3, and smad4. Other sex-related genes such as sf1 were also predicted to be miRNA target genes. Conclusions: This comprehensive miRNA transcriptome analysis demonstrated differential expression profiles of miRNAs during ovary development in common carp. These results could facilitate future exploitation of the sexregulatory roles and mechanisms of miRNAs, especially in primordial gonads, while the specifically expressed miRNAs represent candidates for studying the mechanisms of ovary determination in Yellow River carp. [ABSTRACT FROM AUTHOR]
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- 2017
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44. Inhibition of NAMPT aggravates high fat diet-induced hepatic steatosis in mice through regulating Sirt1/AMPKα/SREBP1 signaling pathway.
- Author
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Ling-Fang Wang, Xiao-Nv Wang, Cong-Cong Huang, Long Hu, Yun-Fei Xiao, Xiao-Hui Guan, Yi-Song Qian, Ke-Yu Deng, and Hong-Bo Xin
- Subjects
- *
NICOTINAMIDE , *PHOSPHORIBOSYLTRANSFERASES , *FATTY degeneration , *HIGH-fat diet , *STEROL regulatory element-binding proteins , *FATTY acid synthases , *CELL metabolism - Abstract
Background: Nonalcoholic fatty liver disease is one of the most common liver diseases in the world and is a typical hepatic manifestation of metabolic syndrome which is characterized with lipid accumulation in liver. Nicotinamide phosphoribosyltransferase (NAMPT) has been recently identified as an enzyme involved in nicotinamide adenine dinucleotide (NAD+) biosynthesis and plays an important role in cellular metabolism in variety of organs in mammals. The aim of this study was to investigate the effects of NAMPT on high fat dietinduced hepatic steatosis. Methods: Hepatic steatosis model was induced by high fat diet (HFD) in C57BL/6 mice in vivo. HepG2 and Hep1-6 hepatocytes were transfected with NAMPT vector plasmid or treated with NAMPT inhibitor FK866 and then incubated with oleic acid. Lipids accumulation was examined by HE staining or oil red staining. Quantitative RT-PCR and Western blot were used to measure expressions of the genes involved in lipogenic synthesis. Results: FK866 significantly promoted liver steatosis in the mice fed with HFD and hepatic lipid accumulation in vitro, accompanied by the increases of the expressions of lipogenic genes such as sterol regulatory elementbinding protein 1 (SREBP1) and fatty acid synthase (FASN). Nicotinamide mononucleotide (NMN) and NAD+ significantly rescued the actions of FK866 in vitro. In contrast, overexpression of NAMPT in HepG2 and Hep1-6 hepatocytes ameliorated hepatic lipid accumulation. In addition, FK866 decreased the protein levels of Sirt1 and phospho-AMPKα in liver of the HFD fed mice. Furthermore, Resveratrol, a Sirt1 activator, significantly reduced lipogenic gene expressions, while EX-527, a Sirt1 specific inhibitor, had the opposite effects. Conclusion: Our results demonstrated that inhibition of NAMPT aggravated the HFD- or oleic acid-induced hepatic steatosis through suppressing Sirt1-mediated signaling pathway. On the one hand, the inhibition of NAMPT reduced the production of NAD+ through inhibiting the NAD+ salvage pathway, resulting in the decrease of Sirt1 activity, and then attenuated the deacetylation of SREBP1 in which the inhibition of SREBP1 activity promoted the expressions of FASN and ACC. On the other hand, the reduced Sirt1 activity alleviated the activation of AMPKα to further enhance SREBP1 activities. [ABSTRACT FROM AUTHOR]
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- 2017
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45. Strategies and cost-effectiveness evaluation of persistent albuminuria screening among high-risk population of chronic kidney disease.
- Author
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Huaiyu Wang, Li Yang, Fang Wang, Luxia Zhang, Wang, Huaiyu, Yang, Li, Wang, Fang, and Zhang, Luxia
- Subjects
MEDICAL screening ,ALBUMINURIA ,CHRONIC kidney failure ,ALBUMINS ,PATIENT acceptance of health care ,HEALTH outcome assessment ,DIAGNOSIS ,MEDICAL care cost statistics ,ATTRIBUTION (Social psychology) ,COST effectiveness ,PROGNOSIS ,SURVIVAL ,COMORBIDITY ,DISEASE prevalence ,EARLY diagnosis ,ECONOMICS - Abstract
Background: Screening for persistent albuminuria among the high-risk population is important for early detection of CKD while studies regarding screening protocol and related cost-effectiveness analysis are limited. This study explored a feasible and cost-efficient screening strategy for detecting persistent albuminuria among the high-risk population.Methods: A cohort study including 157 clinically stable outpatients with a risk factor of CKD and whose laboratory tests revealed an albumin-creatinine-ratio (ACR) between 30 and 300 mg/g of creatinine during the previous 12 months was conducted to assess the validity of alternative screening strategies. Each participant collected three first morning urine samples in three consecutive months. These samples were labeled as DAY-1, MONTH-2 and MONTH-3. In the first month, a random spot sample in the afternoon of the first day and another morning sample on the second day were collected and labeled as Random and DAY-2. Persistent albuminuria was defined by abnormal ACR (≥30 mg/g creatinine) for DAY-1, MONTH-2 and MONTH-3. Alternative strategies were DAY-1; Random; DAY-1 + Random; DAY-1 + DAY-2; and DAY-1 + Random + DAY-2. To evaluate the economic performance of those alternative strategies, a hybrid decision tree/Markov model was developed based on the cohort study to simulate both clinical and cost-effectiveness outcomes. Sensitivity analyses were conducted to investigate assumptions of the model and to examine the model's robustness.Results: Altogether, 82 patients exhibited persistent albuminuria. All of the five strategies had sensitivity higher than 90%. Of these strategies, Random had the lowest specificity (46.7%), and DAY-1 + Random + DAY-2 had the highest specificity (81.3%). Estimated cost for each quality adjusted life year (QALYs) gained were ¥112,335.88 for DAY-1 + Random, ¥8134.69 for Random and ¥10,327.99 for DAY-1 + Random + DAY-2. When compared with DAY-1 strategy, the sensitivity and specificity of which were 100.0 and 69.3%, respectively. DAY-1 + Random + DAY-2 had the highest effectiveness and incremental effectiveness of 11.87 and 0.73 QALYs. At a willingness-to-pay threshold of ¥100,000 per QALY, DAY-1 + Random + DAY-2 had the highest acceptability of 91.0%. Sensitivity analyses demonstrated the robustness of the results.Conclusions: In order to make a quick diagnosis of persistent albuminuria among high-risk population, the strategy of combining two first morning urine samples and one randomized spot urine sample in two consecutive days is accurate, saves time, and is cost-effective. [ABSTRACT FROM AUTHOR]- Published
- 2017
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46. The safe screw path along inferior border of the arcuate line at acetabular area: an anatomical study based on CT scans.
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Chun Bi, Jiandong Wang, Xiaoxi Ji, Zhijian Ma, Fang Wang, Xiangsen Zeng, Dongmei Wang, Qiugen Wang, Bi, Chun, Wang, Jiandong, Ji, Xiaoxi, Ma, Zhijian, Wang, Fang, Zeng, Xiangsen, Wang, Dongmei, and Wang, Qiugen
- Subjects
INTERNAL fixation in fractures ,ACETABULARIA ,HIP fractures ,OSTEOARTHRITIS ,BONE screws ,ACETABULUM surgery ,ACETABULUM (Anatomy) ,COMPUTED tomography ,STANDARDS ,ANATOMY - Abstract
Background: Misplaced screw during the internal fixation of acetabular fractures may penetrate the hip joint which might cause chondrolysis and traumatic osteoarthritis in the future. This study aims to acquire the safe path for screw insertion along inferior border of the arcuate line fixation route at acetabular area.Methods: Computed tomography (CT) scans of 98 patients without pelvic trauma were rebuilt for three-dimensional models of pelvis. After depicting the fixation route curve, five cross-sections perpendicularly to the curve were established from the anterior of pelvis to the posterior along inferior border of the arcuate line. The safe screw lengths for section 1 and 5 were measured from the computer models. In section 2, 3 and 4, a line from the screw entry point tangent to the inferior edge of the acetabulum was depicted and the measurements of minimum safe direction of screw insertion were performed then marked with angle θ.Results: The safe screw lengths for section 1 and 5 were 22.29 ± 4.41 mm and 32.64 ± 4.70 mm (n = 98). The minimum safe angles of screw insertion for the middle three sections 2, 3, and 4 were 65.38 ± 10.23°, 74.20 ± 10.20°, and 57.88 ± 11.11°(n = 98), respectively. The results for the male group (n = 98) indicated smaller minimum safe angles in these three sections compared with the female (n = 98).Conclusions: Compared to male, the minimum safe angles of screw placement at acetabular area for female should be more away from inferior edge of acetabulum and tilt to the bottom of pelvis along inferior border fixation route in surgical management of acetabular fractures. [ABSTRACT FROM AUTHOR]- Published
- 2017
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47. Genetic diagnosis and treatment of a Chinese ketosis-prone MODY 3 family with depression.
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Jun Tang, Chen-Yi Tang, Fang Wang, Yue Guo, Hao-Neng Tang, Ci-La Zhou, Shu-Wen Tan, Shi-Ping Liu, Zhi-Guang Zhou, and Hou-De Zhou
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GENETIC mutation ,CHINESE people ,DIABETES ,MENTAL depression ,PATHOLOGICAL psychology ,MENTAL health - Abstract
Background: To analyze the gene mutation and mental disorder of a Chinese ketosis-prone diabetes (KPD) family, and to make a precise diagnosis and give a treatment for them. Methods: We studied a Chinese family with a clinical diagnosis of maturity-onset diabetes of the young (MODY). The clinical data and the blood samples were collected. The promotor and coding regions inclusive intron exon boundaries of the HNF1A, HNF4A were detected by polymerase chain reaction (PCR) and direct sequencing. The missense mutation was also analyzed by bioinformatics. Genetic counseling was performed twice a month to relieve the mental disorder of the persons. Results: The missense mutation c.779 C>T (p.T260M) in exon4 of HNF1A gene was detected, and the symptom heterogenicity among persons in this family were found. All the members were retreated with Gliclazide and stopped to use other medicine, the blood glucose of them were well controlled. We also performed an active genetic counseling to them and the mental disorder of the proband's sister was relieved. Conclusions: A missense mutation of HNF1A gene was first found in Chinese ketosis-prone MODY family with manifestations heterogenicity among the persons. Sulphonylureas medicine and genetic counseling are efficiency ways to treat MODY 3 and its' mental disorder respectively. [ABSTRACT FROM AUTHOR]
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- 2017
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48. Ecogenomic survey of plant viruses infecting Tobacco by Next generation sequencing.
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Akinyemi, Ibukun A., Fang Wang, Benguo Zhou, Shuishui Qi, and Qingfa Wu
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TOBACCO diseases & pests , *CROP yields , *NON-coding RNA , *PLANT mutation - Abstract
Background: The invasion of plant by viruses cause major damage to plants and reduces crop yield and integrity. Devastating plant virus infection has been experienced at different times all over the world, which are attributed to different events of mutation, re-assortment and recombination occurring in the viruses. Strategies for proper virus management has been mostly limited to eradicating the vectors that spreads the plant viruses. However, development of prompt and effective diagnostic methods are required to monitor emerging and re-emerging diseases that may be symptomatic or asymptomatic in the plant as well as the genetic variation and evolution in the plant viruses. A survey of plant viruses infecting field-grown Tobacco crop was conducted in Anhui Province of China by the deep sequencing of sRNAs. Methods: Survey of plant viruses infecting Tobacco was carried based on 104 samples collected across the province. Nine different sRNA libraries was prepared and custom-made bioinformatics pipeline coupled with molecular techniques was developed to sequence, assemble and analyze the siRNAs for plant virus discovery. We also carried out phylogenetic and recombination analysis of the identified viruses. Results: Twenty two isolates from eight different virus species including Cucumber mosaic virus, Potato virus Y, Tobacco mosaic virus, Tobacco vein banding Mosaic virus, Pepper mottle virus, Brassica yellow virus, Chilli venial mottle virus, Broad bean wilt virus 2 were identified in tobacco across the survey area. The near-complete genome sequence of the 22 new isolates were determined and analyzed. The isolates were grouped together with known strains in the phylogenetic tree. Molecular variation in the isolates indicated the conserved coding regions have majorly a nucleotide sequence identity of 80-94 % with previously identified isolates. Various events of recombination were discovered among some of the isolates indicating that two or more viruses or different isolates of one virus infect the same host cell. Conclusion: This study describes the discovery of a consortium of plant viruses infecting Tobacco that are broadly distributed in Anhui province of China. It also demonstrates the effectiveness of NGS in identifying plant viruses without a prior knowledge of the virus and the genetic diversity that enhanced mixed infection. [ABSTRACT FROM AUTHOR]
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- 2016
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49. Maternal allergic disease history affects childhood allergy development through impairment of neonatal regulatory T-cells.
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Shan-shan Meng, Rong Gao, Bing-di Yan, Jin Ren, Fei Wu, Peng Chen, Jie Zhang, Li-fang Wang, Yuan-ming Xiao, Jing Liu, Meng, Shan-Shan, Gao, Rong, Yan, Bing-di, Ren, Jin, Wu, Fei, Chen, Peng, Zhang, Jie, Wang, Li-Fang, Xiao, Yuan-Ming, and Liu, Jing
- Subjects
ALLERGY in children ,IMMUNOLOGIC diseases in children ,T-cell receptor genes ,NEONATAL diseases ,NEWBORN infant immunology ,DISEASE risk factors ,ALLERGIES ,CELL culture ,CELL physiology ,CELL receptors ,CYTOKINES ,CORD blood ,IMMUNOLOGY technique ,INFLAMMATORY mediators ,INTERLEUKIN-2 ,RESEARCH methodology ,PROTEINS ,QUESTIONNAIRES ,T cells ,TISSUE culture ,PHENOTYPES ,CASE-control method ,CD4 lymphocyte count ,PRENATAL exposure delayed effects ,ODDS ratio ,BLOOD ,DIAGNOSIS - Abstract
Background: Maternal allergic disease history and impaired regulatory T-cells (Tregs) are critical risk factors for allergy development in children. However, the mechanisms that underlie these risk factors remain poorly defined. Therefore, the aim of this study was to assess whether maternal allergies affect the Tregs of offspring and lead to allergy development in childhood.Methods: A total of 332 mothers of healthy newborns (234 from no allergic mothers, 98 from allergic mothers) were recruited to this study. Detailed questionnaires were administered yearly to determine the allergy status of the mothers and the newborns from birth to 3 years of age. Cord blood samples obtained at the time of birth were analysed for Treg counts, as well Treg activity, based on their response to Toll-like receptor (TLR) stimuli such as lipid A (LPA) and peptidoglycans (PPG). Surface markers, associated genes, suppressive capacity, and cytokine levels of Tregs were also measured. Possible correlations between Treg activity and maternal or neonate allergies were assessed. In addition, environmental microbial content and other known risk factors for allergies were measured.Results: Cord blood mononuclear cells (CBMCs) from offspring with allergic mothers showed fewer CD4(+)CD25(+)FOXP3(+) T cells, lower expression levels of associated genes, and reduced cytokine production of interleukin (IL)-10 and interferon-γ (P < 0.05), especially via the PPG-TLR2 pathway. Suppression of effector T cells by Tregs from children of mothers with allergies was impaired, especially IL-13 production by Type 2 T helper (Th2) cells (P = 0.026). Children who developed allergies in the first 3 years of life had lower numbers of CD4(+)CD25(+)FOXP3(+) T cells and reduced FOXP3 expression and IL-10 production as newborns (P < 0.05). Maternal allergic background was identified as a risk factor for allergy development in the children (Odds ratio (OR) = 2.46, 95 % CI = 1.05-5.79); while declining Treg numbers, IL-10 production, and FOXP3 expression in neonates (PPG and LPA stimulated) were identified as independent risk factors for allergic diseases in offspring at 3 years of age after adjusting for maternal allergic history and environmental factors (P < 0.05).Conclusion: Maternal allergy correlated with impaired Tregs in neonates, and this could enhance the susceptibility of offspring to allergic diseases in early childhood due to an imbalance of Th1 and Th2 cells. [ABSTRACT FROM AUTHOR]- Published
- 2016
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50. Efficacy and safety of using a warming needle for persistent allergic rhinitis: study protocol for a randomized controlled trial.
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Yuxiu Sun, Hong Zhao, Yongming Ye, Wenbin Nie, Wenjing Bai, Jia Liu, Sinuo Li, Fang Wang, Mingjuan Han, Liyun He, Sun, Yuxiu, Zhao, Hong, Ye, Yongming, Nie, Wenbin, Bai, Wenjing, Liu, Jia, Li, Sinuo, Wang, Fang, Han, Mingjuan, and He, Liyun
- Subjects
RANDOMIZED controlled trials ,PATIENT compliance ,ACUPUNCTURE ,ALLERGIC rhinitis ,HAY fever treatment ,DIAGNOSIS ,RHINITIS treatment ,RHINITIS ,ANTIHISTAMINES ,COMPARATIVE studies ,EXPERIMENTAL design ,HEAT ,HYPODERMIC needles ,RESEARCH methodology ,MEDICAL cooperation ,RESEARCH protocols ,QUALITY of life ,QUESTIONNAIRES ,RESEARCH ,TIME ,EVALUATION research ,TREATMENT effectiveness ,BLIND experiment ,LORATADINE ,PSYCHOLOGY ,EQUIPMENT & supplies ,THERAPEUTICS - Abstract
Background: Many previous studies have shown the potential therapeutic effect of acupuncture for allergic rhinitis. Most of these studies, however, were limited by the short duration of observations and lack of sham acupuncture as the control group. Our preliminary experiments showed that the use of a warm needling achieved a much more persistent effect in the treatment of allergic rhinitis (AR) compared with simple acupuncture therapy. Hence, we have designed a multicenter, randomized controlled trial (RCT) in which the first-line medication loratadine will be used as the control group, and the effect of warm needling therapy will be evaluated through long-term observation.Methods/design: The trial is designed as a multicenter, parallel-group, randomized, single-blinded (outcome assessors), non-inferiority trial. A total of 98 patients with persistent AR will be randomly assigned into two groups. Patients in the treatment group will be treated with warm needling at GV14 and acupuncture at EX-HN3, ST2, LI20, EX-HN8, GV23, LU7, LU5 and LI4 three times a week, for a total of 4 weeks. Patients in the control group will be treated with oral loratadine 10 mg/day for 4 weeks. The primary outcome will be the change in the Total Nasal Symptom Score (TNSS) from baseline to that at 6 months after treatment during the follow-up period. The secondary outcomes will include the Total Non-nasal Symptom Score and the Rhinoconjunctivitis Quality of Life Questionnaire, changes in the TNSS from baseline to that at 2 and 4 weeks during treatment, and 3 months after treatment during the follow-up period. Outcomes will be measured at 2 and 4 weeks, and 3 and 6 months after treatment. Any side effects of treatment will be observed and recorded.Discussion: We expect that the study results will provide evidence to determine the effects of warm needling compared with loratadine. Our final goal of the study is to evaluate the difference in the short-term and long-term effects between the two therapeutic methods, especially the long-term effect of warm needling.Trial Registration: ClinicalTrials.gov NCT02339714 . Registered on 14 January 2015. [ABSTRACT FROM AUTHOR]- Published
- 2016
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