27 results on '"Feng, Jiao"'
Search Results
2. Anterior chamber and angle characteristics in Chinese children (6–11 years old) with different refractive status using swept-source optical coherence tomography
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Zhao, Li Li, Lu, Xiu Zhen, Tang, Guo Dong, Zhang, Xiu Yan, Li, Run Kuan, Xu, Jing, Feng, Jiao Jiao, Xu, Zhe, Song, Ji Ke, and Bi, Hong Sheng
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- 2024
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3. Construction of cell factory through combinatorial metabolic engineering for efficient production of itaconic acid
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Feng, Jiao, Li, Chunqiu, He, Hao, Xu, Sheng, Wang, Xin, and Chen, Kequan
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- 2022
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4. Nursing allocation in isolation wards of COVID-19 designated hospitals: a nationwide study in China
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Ren, Hong-fei, Chen, Feng-jiao, He, Ling-xiao, Liu, Chang-qing, Liu, Ying-ying, Huang, Yu-jia, Han, Hui, Fu, Su, Zhang, Ming-guang, and Jiang, Yan
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- 2022
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5. Current status of ctDNA in precision oncology for hepatocellular carcinoma
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Li, Yan, Zheng, Yuanyuan, Wu, Liwei, Li, Jingjing, Ji, Jie, Yu, Qiang, Dai, Weiqi, Feng, Jiao, Wu, Jianye, and Guo, Chuanyong
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- 2021
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6. Correction to: Current status of ctDNA in precision oncology for hepatocellular carcinoma
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Li, Yan, Zheng, Yuanyuan, Wu, Liwei, Li, Jingjing, Ji, Jie, Yu, Qiang, Dai, Weiqi, Feng, Jiao, Wu, Jianye, and Guo, Chuanyong
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- 2021
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7. Cellular based immunotherapy for primary liver cancer
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Zheng, Yuanyuan, Li, Yan, Feng, Jiao, Li, Jingjing, Ji, Jie, Wu, Liwei, Yu, Qiang, Dai, Weiqi, Wu, Jianye, Zhou, Yingqun, and Guo, Chuanyong
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- 2021
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8. HELLS serves as a poor prognostic biomarker and its downregulation reserves the malignant phenotype in pancreatic cancer
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Wang, Feng-Jiao, Jing, Yan-Hua, Cheng, Chien-Shan, Cao, Zhang-Qi, Jiao, Ju-Ying, and Chen, Zhen
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- 2021
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9. Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats
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Gu, Wen, Yang, Min, Bi, Qian, Zeng, Lin-Xi, Wang, Xi, Dong, Jin-Cai, Li, Feng-Jiao, Yang, Xing-Xin, Li, Jing-Ping, and Yu, Jie
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- 2020
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10. Emerging roles and the regulation of aerobic glycolysis in hepatocellular carcinoma
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Feng, Jiao, Li, Jingjing, Wu, Liwei, Yu, Qiang, Ji, Jie, Wu, Jianye, Dai, Weiqi, and Guo, Chuanyong
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- 2020
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11. Simvastatin re-sensitizes hepatocellular carcinoma cells to sorafenib by inhibiting HIF-1α/PPAR-γ/PKM2-mediated glycolysis
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Feng, Jiao, Dai, Weiqi, Mao, Yuqing, Wu, Liwei, Li, Jingjing, Chen, Kan, Yu, Qiang, Kong, Rui, Li, Sainan, Zhang, Jie, Ji, Jie, Wu, Jianye, Mo, Wenhui, Xu, Xuanfu, and Guo, Chuanyong
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- 2020
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12. PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma
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Feng, Jiao, Wu, Liwei, Ji, Jie, Chen, Kan, Yu, Qiang, Zhang, Jie, Chen, Jiaojiao, Mao, Yuqing, Wang, Fan, Dai, Weiqi, Xu, Ling, Wu, Jianye, and Guo, Chuanyong
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- 2019
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- View/download PDF
13. Nursing allocation in isolation wards of COVID-19 designated hospitals: a nationwide study in China
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Hong-fei Ren, Feng-jiao Chen, Ling-xiao He, Chang-qing Liu, Ying-ying Liu, Yu-jia Huang, Hui Han, Su Fu, Ming-guang Zhang, and Yan Jiang
- Subjects
Nurse work hours per shift ,Nursing workforce allocation ,Research ,RT1-120 ,Bed-to-nurse ratio ,COVID-19 ,Nursing ,Designated hospital ,General Nursing - Abstract
Background Appropriate allocation of nursing staff is key to ensuring efficient nursing in hospitals, and is significantly correlated with patient safety, nursing quality, and nurse job satisfaction. However, there are few studies on nursing workforce allocation in the isolation wards of COVID-19 designated hospitals globally. This study aims to better understand the nursing workforce allocation in the isolation wards of COVID-19 designated hospitals in China, and provide a theoretical basis for efficiently deploying first-line nurses in China and across the world in the future. Methods An online survey was conducted among the head nurses (n = 229) and nurses (n = 1378) in the isolation wards of 117 hospitals (selected by stratified sampling), using a self-reported human resource allocation questionnaire. Results The average bed-to-nurse ratios of different isolation wards were different (Z = 36.742, P = 0.000). The bed-to-nurse ratios of the ICU, suspected COVID-19 cases ward, and confirmed COVID-19 cases ward, were 1:1.88, 1:0.56, and 1:0.45, respectively. The nurse work hours per shift in different isolation wards were also different (Z = 8.468, P = 0.014), with the specific values of the ICU, suspected COVID-19 cases ward, and confirmed COVID-19 cases ward, being 5, 6, and 6 h, respectively. A correlation analysis showed that the average work hours per shift was proportional to the overtime work of nurses (rs = 0.146), the proportion of nurse practitioners was proportional to the overall utilization rate of nursing human resources in the wards (rs = 0.136), and the proportion of nurses with college degrees was proportional to teamwork (rs = 0.142). The proportion of nurses above grade 10 was inversely proportional to teamwork and psychological problems (rs = 0.135, rs = 0.203). The results of multiple stepwise regression analyses showed that the work hours of nurses per shift was the main factor affecting nurse satisfaction and that the proportion of nurses and the work hours of nurses per shift were both independent factors affecting the length of stay (LOS) of patients. Conclusion Hospitals in China have made good nursing workforce allocations during the COVID-19 pandemic, but there are certain shortcomings. Therefore, scientific and efficient nursing workforce allocation practice plans should be established to improve the ability of hospitals to deal with public health emergencies and are urgent problems that need to be addressed soon.
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- 2022
14. Involvement of Leptin Receptor Long Isoform (LepRb)-STAT3 Signaling Pathway in Brain Fat Mass- and Obesity-Associated (FTO) Downregulation during Energy Restriction
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Wang, Pei, Yang, Feng-Jiao, Du, Hui, Guan, Yun-Feng, Xu, Tian-Ying, Xu, Xue-Wen, Su, Ding-Feng, and Miao, Chao-Yu
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- 2011
- Full Text
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15. Water extract from processed Polygonum multiflorum modulate gut microbiota and glucose metabolism on insulin resistant rats
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Min Yang, Feng-Jiao Li, Xing-Xin Yang, Jie Yu, Jin-Cai Dong, Jing-Ping Li, Wen Gu, Lin-Xi Zeng, Qian Bi, and Xi Wang
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0301 basic medicine ,Blood Glucose ,medicine.medical_specialty ,Polygonum ,Gut microbiota ,Gut flora ,Carbohydrate metabolism ,Glucagon ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Insulin resistance ,Western blot ,Internal medicine ,medicine ,Animals ,Glucose metabolism ,030109 nutrition & dietetics ,biology ,Triglyceride ,medicine.diagnostic_test ,Plant Extracts ,Processed Polygonum multiflorum ,AMPK ,lcsh:Other systems of medicine ,biology.organism_classification ,medicine.disease ,lcsh:RZ201-999 ,Gastrointestinal Microbiome ,Rats ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,Complementary and alternative medicine ,chemistry ,Fallopia multiflora ,Research Article - Abstract
Background The incidence of insulin resistance (IR) has rapidly increased worldwide over the last 20 years, no perfect solution has yet been identified. Finding new therapeutic drugs will help improve this situation. As a traditional Chinese medicine, PPM (processed Polygonum multiflorum) has widely been used in the clinic. Recently, other clinical functions of PPM have been widely analyzed. Results Administration of the water extract from PPM decreased the level of FBG, TC, and TG, and increased the level of FGC, thereby reducing the IR index and improving IR. Furthermore, Western blot analysis revealed that PPM significantly increased GPR43 and AMPK expression when compared with the MOD group, and GPR43, AMPK were known as glucose metabolism-related proteins. In addition, treatment with PPM can restore the balance of gut microbiota by adjusting the relative abundance of bacteria both at the phylum and genus level, and these changes have been reported to be related to IR. Methods Sprague Dawley (SD) rats were fed a high-fat diet and were gavaged daily with either normal saline solution or PPM for 12 weeks. Major biochemical indexes, such as fasting blood glucose (FBG), fasting glucagon (FGC), total cholesterol (TC), and triglyceride (TG) were measured. Then the protein expression of adenosine 5′-monophosphate -activated protein kinase (AMPK) and G protein-coupled receptor 43 (GPR43) was evaluated by using Western blot analysis. Moreover, the composition of gut microbiota was assessed by analyzing 16S rRNA sequences. Conclusions Our findings showed that PPM reversed the increasing of FBG and the decreasing of IRI, PPM accelerated the expression of glucose metabolism-related proteins and regulated the intestinal microecological balance. Therefore, we hold the opinion that PPM may be an effective option for treating IR.
- Published
- 2020
16. A second puncture and injection technique for treating osteoporotic vertebral compression fractures
- Author
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Feng-Wei Qin, Wang Tang, Yonghui Feng, Sineng Zhang, Weiguang Tan, Dong-Hua Liu, He Huang, Chun-Liang Xie, Zhao-Fei Zhang, Feng Jiao, and Pei-Yu Wu
- Subjects
Male ,medicine.medical_specialty ,Osteoporotic vertebral compression fractures ,lcsh:Diseases of the musculoskeletal system ,Visual analogue scale ,medicine.medical_treatment ,Punctures ,Group B ,Cement distribution ,Thoracic Vertebrae ,Injections ,Percutaneous vertebroplasty ,lcsh:Orthopedic surgery ,Fractures, Compression ,medicine ,Humans ,Orthopedics and Sports Medicine ,Second puncture and injection ,Aged ,Retrospective Studies ,Aged, 80 and over ,Vertebroplasty ,Cement leakage ,Lumbar Vertebrae ,business.industry ,Bone Cements ,Middle Aged ,Compression (physics) ,Oswestry Disability Index ,Surgery ,lcsh:RD701-811 ,Vertebral height ,Orthopedic surgery ,Spinal Fractures ,Female ,lcsh:RC925-935 ,business ,Osteoporotic Fractures ,Research Article ,Follow-Up Studies - Abstract
Objective To evaluate the clinical effect of the second puncture and injection technique during a percutaneous vertebroplasty (PVP) procedure. Methods Patients treated with a second puncture and injection (group A) or a single puncture and injection (group B) during PVP at our institution during 2010–2017 were reviewed. Vertebral height loss, visual analogue scale (VAS) score, Oswestry disability index (ODI), adjacent vertebral fractures, and cement leakage were compared between the groups. Results A total of 193 patients were enrolled (86 cases in group A, 107 cases in group B). The follow-up period was 15.64 (12–20) months. The loss of anterior (group A 0.01 ± 0.03; group B 0.14 ± 0.17) and middle (group A 0.13 ± 0.12; group B 0.16 ± 0.11) vertebral height in group B was significantly higher than that in group A (P < 0.05). The VAS score and ODI were also significantly higher in group B than in group A at the final follow-up; the VAS score and ODI in group B were 1.65 ± 0.70 and 14.50 ± 4.16, respectively, and those in group A were 1.00 ± 0.74 and 12.81 ± 4.02, respectively (P < 0.05). Three patients in group A and two in group B experienced adjacent vertebral fractures. Regarding mild, moderate, and severe cement leakage, there were 25 (29%), 5 (5%), and 0 cases, respectively, in group A and 28 (26%), 3 (2.8%), and 1 (0.009%) case, respectively, in group B (P > 0.05). Conclusions The second puncture and injection technique may effectively increase the dispersion of cement, thus preventing recompression of the cemented vertebral body, and it does not increase the risk of cement leakage or adjacent vertebral fracture.
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- 2019
17. A novel feedback loop between high MALAT-1 and low miR-200c-3p promotes cell migration and invasion in pancreatic ductal adenocarcinoma and is predictive of poor prognosis
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Meng Zhuo, Liwei Wang, Feng Jiao, Cuncun Yuan, Jiujie Cui, and Ting Han
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0301 basic medicine ,Cancer Research ,Biology ,Metastasis-associated lung adenocarcinoma transcript 1 ,lcsh:RC254-282 ,Cell Line ,Pancreatic ductal adenocarcinoma ,03 medical and health sciences ,miR-200c-3p ,Downregulation and upregulation ,Cell Movement ,microRNA ,Genetics ,medicine ,Biomarkers, Tumor ,Gene silencing ,ZEB1 ,Humans ,Cell Proliferation ,Cell growth ,Competing endogenous RNA ,Zinc Finger E-box-Binding Homeobox 1 ,Cell migration ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Prognosis ,Blot ,Pancreatic Neoplasms ,MicroRNAs ,030104 developmental biology ,Oncology ,Cancer research ,Adenocarcinoma ,RNA, Long Noncoding ,Feedback loop ,Research Article ,Carcinoma, Pancreatic Ductal - Abstract
Background It was demonstrated that long non-coding RNAs occupied an important position in tumor pathogenesis and progression. We have previously found that the metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) promotes cell proliferation and metastases in pancreatic ductal adenocarcinoma (PDAC). The present study was aimed to discuss the underlying mechanisms. Methods Bioinformatics method was used to identify the miRNA target of MALAT-1. Expressions of relative genes were assessed by quantitative real-time PCR and western blotting, respectively. Sulforhodamine B assay and Transwell assay were employed to detect cell proliferation, migration and invasion, respectively. Moreover, RNA immunoprecipitation was performed to determine whether RNA-induced silencing complex contained MALAT-1 and its potential binding miRNA. Luciferase assays was used to confirm potential binding site. Results Bioinformatics search predicted that miR-200c-3p was a direct target of MALAT-1. Further, we found a reciprocal suppression between MALAT-1 and miR-200c-3p expression. In terms of mechanisms, high MALAT-1 and low miR-200c-3p may form a novel feedback loop. On the one hand, MALAT-1 functioned as a competing endogenous RNA to suppress miR-200c-3p expression, leading to upregulation of ZEB1 expression. On the other hand, miR-200c-3p inhibited the level of MALAT-1 expression was in a way similar to miRNA-mediated downregulation of target genes. Clinical data further indicated that MALAT-1 and ZEB1 expression was negatively correlated with miR-200c-3p transcript level of PDAC tissues. There was a positive correlation between MALAT-1 and ZEB1 level. MALAT-1 (high)/miR-200c-3p (low) correlated with shorter overall survival of PDAC patients. Multivariate analysis revealed that both MALAT-1 and miR-200c-3p levels were independent prognostic factors. Conclusion Our findings firstly revealed a novel feedback loop between high MALAT-1 and low miR-200c-3p. Targeting the feedback loop between high MALAT-1 and low miR-200c-3p will be a therapeutic strategy for PDAC. Electronic supplementary material The online version of this article (10.1186/s12885-018-4954-9) contains supplementary material, which is available to authorized users.
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- 2018
18. Process optimization for enhancing production of cis-4-hydroxy-l-proline by engineered Escherichia coli
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Bowen Zhang, Feng Jiao, Sheng Xu, Yang Pang, Xin Wang, Pingkai Ouyang, and Kequan Chen
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0301 basic medicine ,Proline ,lcsh:QR1-502 ,Gene Expression ,Bioengineering ,Biology ,CHOP ,Two-strain coupling system ,medicine.disease_cause ,Hydroxylation ,Applied Microbiology and Biotechnology ,lcsh:Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Biosynthesis ,Biotransformation ,Dioxygenase ,medicine ,Escherichia coli ,Bioprocess ,cis-4-Hydroxy-l-proline ,Research ,Process limitations ,030104 developmental biology ,chemistry ,Biochemistry ,Genetic Engineering ,Biotechnology - Abstract
Background Understanding the bioprocess limitations is critical for the efficient design of biocatalysts to facilitate process feasibility and improve process economics. In this study, a proline hydroxylation process with recombinant Escherichia coli expressing l-proline cis-4-hydroxylase (SmP4H) was investigated. The factors that influencing the metabolism of microbial hosts and process economics were focused on for the optimization of cis-4-hydroxy-l-proline (CHOP) production. Results In recombinant E. coli, SmP4H synthesis limitation was observed. After the optimization of expression system, CHOP production was improved in accordance with the enhanced SmP4H synthesis. Furthermore, the effects of the regulation of proline uptake and metabolism on whole-cell catalytic activity were investigated. The improved CHOP production by repressing putA gene responsible for l-proline degradation or overexpressing l-proline transporter putP on CHOP production suggested the important role of substrate uptake and metabolism on the whole-cell biocatalyst efficiency. Through genetically modifying these factors, the biocatalyst activity was significantly improved, and CHOP production was increased by twofold. Meanwhile, to further improve process economics, a two-strain coupling whole-cell system was established to supply co-substrate (α-ketoglutarate, α-KG) with a cheaper chemical l-glutamate as a starting material, and 13.5 g/L of CHOP was successfully produced. Conclusions In this study, SmP4H expression, and l-proline uptake and degradation, were uncovered as the hurdles for microbial production of CHOP. Accordingly, the whole-cell biocatalysts were metabolically engineered for enhancing CHOP production. Meanwhile, a two-strain biotransformation system for CHOP biosynthesis was developed aiming at supplying α-KG more economically. Our work provided valuable insights into the design of recombinant microorganism to improve the biotransformation efficiency that catalyzed by Fe(II)/α-KG-dependent dioxygenase. Electronic supplementary material The online version of this article (10.1186/s12934-017-0821-7) contains supplementary material, which is available to authorized users.
- Published
- 2017
19. Follicular thyroid carcinoma but not adenoma recruits tumor-associated macrophages by releasing CCL15.
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Feng-Jiao Huang, Xiao-Yi Zhou, Lei Ye, Xiao-Chun Fei, Shu Wang, Weiqing Wang, Guang Ning, Huang, Feng-Jiao, Zhou, Xiao-Yi, Ye, Lei, Fei, Xiao-Chun, Wang, Shu, Wang, Weiqing, and Ning, Guang
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- *
THYROID cancer , *MACROPHAGES , *ADENOMA , *CANCER cells , *IMMUNOSTAINING , *CHEMOTAXIS , *ADENOCARCINOMA , *CYTOKINES , *DIFFERENTIAL diagnosis , *GENES , *NEEDLE biopsy , *PROTEINS , *RNA , *PREOPERATIVE period , *TISSUE arrays , *DIAGNOSIS - Abstract
Background: The differential diagnosis of follicular thyroid carcinoma (FTC) and follicular adenoma (FA) before surgery is a clinical challenge. Many efforts have been made but most focusing on tumor cells, while the roles of tumor associated macrophages (TAMs) remained unclear in FTC. Here we analyzed the differences between TAMs in FTC and those in FA.Methods: We first analyzed the density of TAMs by CD68 immunostaining in 59 histologically confirmed FTCs and 47 FAs. Cytokines produced by FTC and FA were profiled using antibody array, and validated by quantitative PCR. Chemotaxis of monocyte THP-1 was induced by condition medium of FTC cell lines (FTC133 and WRO82-1) with and without anti-CCL15 neutralizing antibody. Finally, we analyzed CCL15 protein level in FTC and FA by immunohistochemistry.Results: The average density of CD68(+) cells was 9.5 ± 5.4/field in FTC, significantly higher than that in FA (4.9 ± 3.4/field, p < 0.001). Subsequently profiling showed that CCL15 was the most abundant chemokine in FTC compared with FA. CCL15 mRNA in FTC was 51.4-folds of that in FA. CM of FTC cell lines induced THP-1 cell chemotaxis by 33 ~ 77%, and anti-CCL15 neutralizing antibody reduced THP-1 cell migration in a dose-dependent manner. Moreover, we observed positive CCL15 immunostaining in 67.8% of FTCs compared with 23.4% of FAs.Conclusion: Our study suggested FTC might induce TAMs infiltration by producing CCL15. Measurement of TAMs and CCL15 in follicular thyroid lesions may be applied clinically to differentiate FTC from FA pre-operation. [ABSTRACT FROM AUTHOR]- Published
- 2016
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20. Association of a novel point mutation in MSH2 gene with familial multiple primary cancers.
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Hai Hu, Hong Li, Feng Jiao, Ting Han, Meng Zhuo, Jiujie Cui, Yixue Li, and Liwei Wang
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CANCER ,POINT mutation (Biology) ,MSH2 gene ,HEREDITARY nonpolyposis colorectal cancer ,GENETIC disorders - Abstract
Background: Multiple primary cancers (MPC) have been identified as two or more cancers without any subordinate relationship that occur either simultaneously or metachronously in the same or different organs of an individual. Lynch syndrome is an autosomal dominant genetic disorder that increases the risk of many types of cancers. Lynch syndrome patients who suffer more than two cancers can also be considered as MPC; patients of this kind provide unique resources to learn how genetic mutation causes MPC in different tissues. Methods: We performed a whole genome sequencing on blood cells and two tumor samples of a Lynch syndrome patient who was diagnosed with five primary cancers. The mutational landscape of the tumors, including somatic point mutations and copy number alternations, was characterized. We also compared Lynch syndrome with sporadic cancers and proposed a model to illustrate the mutational process by which Lynch syndrome progresses to MPC. Results: We revealed a novel pathologic mutation on the MSH2 gene (G504 splicing) that associates with Lynch syndrome. Systematical comparison of the mutation landscape revealed that multiple cancers in the proband were evolutionarily independent. Integrative analysis showed that truncating mutations of DNA mismatch repair (MMR) genes were significantly enriched in the patient. A mutation progress model that included germline mutations of MMR genes, double hits of MMR system, mutations in tissue-specific driver genes, and rapid accumulation of additional passenger mutations was proposed to illustrate how MPC occurs in Lynch syndrome patients. Conclusion: Our findings demonstrate that both germline and somatic alterations are driving forces of carcinogenesis, which may resolve the carcinogenic theory of Lynch syndrome. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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21. Leaf cDNA-AFLP analysis of two citrus species differing in manganese tolerance in response to long-term manganese-toxicity.
- Author
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Chen-Ping Zhou, Yi-Ping Qi, Xiang You, Lin-Tong Yang, Peng Guo, Xin Ye, Xin-Xing Zhou, Feng-Jiao Ke, and Li-Song Chen
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GENETIC transduction ,MICROBIAL genetics ,BACTERIOPHAGES ,PROTEIN metabolism ,BIOMOLECULES - Abstract
Background: Very little is known about manganese (Mn)-toxicity-responsive genes in citrus plants. Seedlings of 'Xuegan' (Citrus sinensis) and 'Sour pummelo' (Citrus grandis) were irrigated for 17 weeks with nutrient solution containing 2 μM (control) or 600 μM (Mn-toxicity) MnSO4. The objectives of this study were to understand the mechanisms of citrus Mn-tolerance and to identify differentially expressed genes, which might be involved in Mn-tolerance. Results: Under Mn-toxicity, the majority of Mn in seedlings was retained in the roots; C. sinensis seedlings accumulated more Mn in roots and less Mn in shoots (leaves) than C. grandis ones and Mn concentration was lower in Mn-toxicity C. sinensis leaves compared to Mn-toxicity C. grandis ones. Mn-toxicity affected C. grandis seedling growth, leaf CO2 assimilation, total soluble concentration, phosphorus (P) and magenisum (Mg) more than C. sinensis. Using cDNA-AFLP, we isolated 42 up-regulated and 80 down-regulated genes in Mn-toxicity C. grandis leaves. They were grouped into the following functional categories: biological regulation and signal transduction, carbohydrate and energy metabolism, nucleic acid metabolism, protein metabolism, lipid metabolism, cell wall metabolism, stress responses and cell transport. However, only 7 up-regulated and 8 down-regulated genes were identified in Mn-toxicity C. sinensis ones. The responses of C. grandis leaves to Mn-toxicity might include following several aspects: (1) accelerating leaf senescence; (2) activating the metabolic pathway related to ATPase synthesis and reducing power production; (3) decreasing cell transport; (4) inhibiting protein and nucleic acid metabolisms; (5) impairing the formation of cell wall; and (6) triggering multiple signal transduction pathways. We also identified many new Mn-toxicity-responsive genes involved in biological and signal transduction, carbohydrate and protein metabolisms, stress responses and cell transport. Conclusions: Our results demonstrated that C. sinensis was more tolerant to Mn-toxicity than C. grandis, and that Mn-toxicity affected gene expression far less in C. sinensis leaves. This might be associated with more Mn accumulation in roots and less Mn accumulation in leaves of Mn-toxicity C. sinensis seedlings than those of C. grandis seedlings. Our findings increase our understanding of the molecular mechanisms involved in the responses of plants to Mn-toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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22. Patterns and socioeconomic influences of tobacco exposure in tobacco cultivating rural areas of Yunnan Province, China.
- Author
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Le Cai, Xinan Wu, Abhinav Goyal, Yuntao Han, Wenlong Cui, Xia Xiao, Jianhui He, Keying Zhao, Ying Song, and Feng Jiao
- Subjects
TOBACCO ,CIGARETTE smokers ,NICOTINE ,MULTICULTURALISM - Abstract
Background: This study describes the patterns and socioeconomic influences of tobacco use among adults in tobacco-cultivating regions of rural southwest China. Methods: A cross-sectional survey was conducted in 8681 adults aged ?18 years in rural areas of Yunnan Province, China from 2010 to 2011. A standardized questionnaire was administered to obtain data about participants' demographic characteristics, individual socioeconomic status, ethnicity, self-reported smoking habits, and exposure to secondhand smoke (SHS). The socioeconomic predictors of current smoking, nicotine addiction, and SHS exposure were analyzed using multivariate logistic regression. Results: The prevalence rates of tobacco use were much higher in men compared with women (current smoking 68.5% vs. 1.3%; and nicotine dependence 85.2% vs. 72.7%). However, the rate of SHS exposure was higher in women compared with men (76.6% vs. 70.5%). Tobacco farmers had higher prevalence rates of current smoking, nicotine dependence, and SHS exposure compared with participants not engaged in tobacco farming (P<0.01). Most tobacco users (84.5%) reported initiating smoking during adolescence. A total of 81.1% of smokers smoked in public places, and 77.6% smoked in workplaces. Individuals belonging to an ethnic minority had a lower probability of SHS exposure and nicotine dependence. Individual educational level was found to be inversely associated with the prevalence of current smoking, exposure to SHS, and nicotine dependence. Higher annual household income was associated with a greater risk of nicotine dependence. Conclusions: This study suggests that tobacco control efforts in rural southwest China must be tailored to address tobacco-cultivating status and socioeconomic factors. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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23. Involvement of Leptin Receptor Long Isoform (LepRb)-STAT3 Signaling Pathway in Brain Fat Mass-and Obesity-Associated (FTO) Downregulation during Energy Restriction.
- Author
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Pei Wang, Feng-Jiao Yang, Hui Du, Yun-Feng Guan, Tian-Ying Xu, Xue-Wen Xu, Ding-Feng Su, and Chao-Yu Miao
- Subjects
- *
OBESITY risk factors , *CARDIOVASCULAR diseases , *DIABETES , *LEPTIN , *PEPTIDE hormones , *LABORATORY mice - Abstract
Obesity is an important risk factor for cardiovascular disease, diabetes and certain cancers. The fat mass- and obesity-associated (FTO) gene is tightly associated with the pathophysiology of obesity, whereas the exact role of FTO remains poorly understood. Here, we investigated the alternations of FTO mRNA and protein expression in the peripheral metabolic tissues and the brain upon energy restriction (ER) and explored the involvement of the leptin signaling pathway in FTO regulation under ER status. ER decreased the FTO mRNA and protein expression in hypothalamus and brainstem but not in periphery. Using double-immunofluorescence staining, FTO was found to be colocalized with the leptin receptor long isoform (LepRb) in arcuate nucleus of hypothalamus and the nucleus of the solitary tract. In LepRb mutant db/db mice, the FTO downregulation in brain and body weight reduction induced by ER were completely abolished. The enhanced phosphorylation of signal transducer and activator of transcription 3 (STAT3) induced by ER was also impaired in db/db mice. Moreover, leptin directly activated the STAT3 signaling pathway and downregulated FTO in in vitro arcuate nucleus of hypothalamus cultures and in vivo wild-type mice but not db/db mice. Thus, our results provide the first evidence that the LepRb-STAT3 signaling pathway is involved in the brain FTO downregulation during ER. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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24. Process optimization for enhancing production of cis-4-hydroxy-L-proline by engineered Escherichia coli.
- Author
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Chen K, Pang Y, Zhang B, Feng J, Xu S, Wang X, and Ouyang P
- Subjects
- Gene Expression, Hydroxylation, Escherichia coli chemistry, Genetic Engineering methods, Proline metabolism
- Abstract
Background: Understanding the bioprocess limitations is critical for the efficient design of biocatalysts to facilitate process feasibility and improve process economics. In this study, a proline hydroxylation process with recombinant Escherichia coli expressing L-proline cis-4-hydroxylase (SmP4H) was investigated. The factors that influencing the metabolism of microbial hosts and process economics were focused on for the optimization of cis-4-hydroxy-L-proline (CHOP) production., Results: In recombinant E. coli, SmP4H synthesis limitation was observed. After the optimization of expression system, CHOP production was improved in accordance with the enhanced SmP4H synthesis. Furthermore, the effects of the regulation of proline uptake and metabolism on whole-cell catalytic activity were investigated. The improved CHOP production by repressing putA gene responsible for L-proline degradation or overexpressing L-proline transporter putP on CHOP production suggested the important role of substrate uptake and metabolism on the whole-cell biocatalyst efficiency. Through genetically modifying these factors, the biocatalyst activity was significantly improved, and CHOP production was increased by twofold. Meanwhile, to further improve process economics, a two-strain coupling whole-cell system was established to supply co-substrate (α-ketoglutarate, α-KG) with a cheaper chemical L-glutamate as a starting material, and 13.5 g/L of CHOP was successfully produced., Conclusions: In this study, SmP4H expression, and L-proline uptake and degradation, were uncovered as the hurdles for microbial production of CHOP. Accordingly, the whole-cell biocatalysts were metabolically engineered for enhancing CHOP production. Meanwhile, a two-strain biotransformation system for CHOP biosynthesis was developed aiming at supplying α-KG more economically. Our work provided valuable insights into the design of recombinant microorganism to improve the biotransformation efficiency that catalyzed by Fe(II)/α-KG-dependent dioxygenase.
- Published
- 2017
- Full Text
- View/download PDF
25. Genetic characterization of novel class 1 Integrons In0, In1069 and In1287 to In1290, and the inference of In1069-associated integron evolution in Enterobacteriaceae .
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Wang D, Zhu J, Zhou K, Chen J, Yin Z, Feng J, Ma L, and Zhou D
- Abstract
Background: This study aims to characterize genetically related class 1 integrons In1069, In893 and In1287 to In1290, and to further propose a scheme of stepwise integration or excision of individual gene cassettes (GCs) to generation of these integron variations., Methods: Six of 139 non-redundant Enterobacteriaceae strains were studied by bacterial antimicrobial susceptibility testing, detection of carbapenemase activity, and integron sequencing and sequence comparison., Results: Six novel class 1 integrons, In0, In1069, and In1287 to In1290, together with the previously characterized In893, were determined from the above strains. An unusual bla
KPC-2 -carrying In0 and the blaIMP-30 -carrying In1069 coexists in a single isolate of Escherichia coli . In0 contains a PcH1 promoter and a truncated aacA4'-3 gene cassette (GC aacA4'-3 ), as well as a blaKPC-2 -containing region of Tn 6296 integrated between PcH1 and GC aacA4'-3 . In1069 carries GC blaIMP-30 and GC aacA4'-3 in this order. The other five integrons, In893 and In1287 to In1290, are genetically related to In1069, and all possess a core GC aacA4'-3 . The integration or excision of one or more individual gene cassettes, such as GC blaIMP-30 , GC aadA16 , GC catB3 , GC arr3 and GC dfrA27 , upstream or downstream of GC aacA4'-3 generates various gene cassettes arrays among these five integrons., Conclusions: These findings provide the insight into stepwise and parallel evolution of In1069-associated integron variations likely under antibiotic selection pressure in clinical settings.- Published
- 2017
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26. Follicular thyroid carcinoma but not adenoma recruits tumor-associated macrophages by releasing CCL15.
- Author
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Huang FJ, Zhou XY, Ye L, Fei XC, Wang S, Wang W, and Ning G
- Subjects
- Adenocarcinoma, Follicular genetics, Adenocarcinoma, Follicular pathology, Adenoma genetics, Adenoma pathology, Biopsy, Fine-Needle, Chemokines, CC genetics, Female, Gene Expression Regulation, Neoplastic, Humans, Macrophage Inflammatory Proteins genetics, Macrophages pathology, Male, Preoperative Period, RNA, Messenger biosynthesis, Tissue Array Analysis, Adenocarcinoma, Follicular diagnosis, Adenoma diagnosis, Chemokines, CC biosynthesis, Diagnosis, Differential, Macrophage Inflammatory Proteins biosynthesis
- Abstract
Background: The differential diagnosis of follicular thyroid carcinoma (FTC) and follicular adenoma (FA) before surgery is a clinical challenge. Many efforts have been made but most focusing on tumor cells, while the roles of tumor associated macrophages (TAMs) remained unclear in FTC. Here we analyzed the differences between TAMs in FTC and those in FA., Methods: We first analyzed the density of TAMs by CD68 immunostaining in 59 histologically confirmed FTCs and 47 FAs. Cytokines produced by FTC and FA were profiled using antibody array, and validated by quantitative PCR. Chemotaxis of monocyte THP-1 was induced by condition medium of FTC cell lines (FTC133 and WRO82-1) with and without anti-CCL15 neutralizing antibody. Finally, we analyzed CCL15 protein level in FTC and FA by immunohistochemistry., Results: The average density of CD68(+) cells was 9.5 ± 5.4/field in FTC, significantly higher than that in FA (4.9 ± 3.4/field, p < 0.001). Subsequently profiling showed that CCL15 was the most abundant chemokine in FTC compared with FA. CCL15 mRNA in FTC was 51.4-folds of that in FA. CM of FTC cell lines induced THP-1 cell chemotaxis by 33 ~ 77%, and anti-CCL15 neutralizing antibody reduced THP-1 cell migration in a dose-dependent manner. Moreover, we observed positive CCL15 immunostaining in 67.8% of FTCs compared with 23.4% of FAs., Conclusion: Our study suggested FTC might induce TAMs infiltration by producing CCL15. Measurement of TAMs and CCL15 in follicular thyroid lesions may be applied clinically to differentiate FTC from FA pre-operation.
- Published
- 2016
- Full Text
- View/download PDF
27. Leaf cDNA-AFLP analysis of two citrus species differing in manganese tolerance in response to long-term manganese-toxicity.
- Author
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Zhou CP, Qi YP, You X, Yang LT, Guo P, Ye X, Zhou XX, Ke FJ, and Chen LS
- Subjects
- Citrus physiology, DNA, Complementary genetics, Gene Expression Regulation, Plant, Genes, Plant, Plant Leaves genetics, Plant Roots physiology, Seedlings physiology, Amplified Fragment Length Polymorphism Analysis, Citrus genetics, Manganese toxicity, Plant Leaves physiology
- Abstract
Background: Very little is known about manganese (Mn)-toxicity-responsive genes in citrus plants. Seedlings of 'Xuegan' (Citrus sinensis) and 'Sour pummelo' (Citrus grandis) were irrigated for 17 weeks with nutrient solution containing 2 μM (control) or 600 μM (Mn-toxicity) MnSO₄. The objectives of this study were to understand the mechanisms of citrus Mn-tolerance and to identify differentially expressed genes, which might be involved in Mn-tolerance., Results: Under Mn-toxicity, the majority of Mn in seedlings was retained in the roots; C. sinensis seedlings accumulated more Mn in roots and less Mn in shoots (leaves) than C. grandis ones and Mn concentration was lower in Mn-toxicity C. sinensis leaves compared to Mn-toxicity C. grandis ones. Mn-toxicity affected C. grandis seedling growth, leaf CO₂ assimilation, total soluble concentration, phosphorus (P) and magenisum (Mg) more than C. sinensis. Using cDNA-AFLP, we isolated 42 up-regulated and 80 down-regulated genes in Mn-toxicity C. grandis leaves. They were grouped into the following functional categories: biological regulation and signal transduction, carbohydrate and energy metabolism, nucleic acid metabolism, protein metabolism, lipid metabolism, cell wall metabolism, stress responses and cell transport. However, only 7 up-regulated and 8 down-regulated genes were identified in Mn-toxicity C. sinensis ones. The responses of C. grandis leaves to Mn-toxicity might include following several aspects: (1) accelerating leaf senescence; (2) activating the metabolic pathway related to ATPase synthesis and reducing power production; (3) decreasing cell transport; (4) inhibiting protein and nucleic acid metabolisms; (5) impairing the formation of cell wall; and (6) triggering multiple signal transduction pathways. We also identified many new Mn-toxicity-responsive genes involved in biological and signal transduction, carbohydrate and protein metabolisms, stress responses and cell transport., Conclusions: Our results demonstrated that C. sinensis was more tolerant to Mn-toxicity than C. grandis, and that Mn-toxicity affected gene expression far less in C. sinensis leaves. This might be associated with more Mn accumulation in roots and less Mn accumulation in leaves of Mn-toxicity C. sinensis seedlings than those of C. grandis seedlings. Our findings increase our understanding of the molecular mechanisms involved in the responses of plants to Mn-toxicity.
- Published
- 2013
- Full Text
- View/download PDF
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