41 results on '"Hong Cheng"'
Search Results
2. Effects of neoadjuvant radiochemotherapy for anorectal function in locally advanced rectal cancer patients: a study protocol for a prospective, observational, controlled, multicentre study
- Author
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Shi, Jie, Cheng, Yi-Kan, He, Fang, Zheng, Jian, Wang, Yun-Long, Wan, Xiang-Bo, Lin, Hong-Cheng, and Fan, Xin-Juan
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- 2023
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- View/download PDF
3. The management pattern and outcomes of chronic thromboembolic pulmonary hypertension: rationale and design for a Chinese real-world study
- Author
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Zhang, Shuai, Li, Yishan, Yang, Yuanhua, Gong, Sugang, Yang, Zhenwen, Hong, Cheng, Cui, Xiaopei, Wan, Jun, Ji, Yingqun, Chen, Hong, Zhu, Ling, Li, Chenghong, Cheng, Zhaozhong, Zhang, Yunhui, Wang, Qiguang, Luo, Qin, Guo, Lu, Ma, Guofeng, Han, Baoshi, Liu, Zhihong, Xiong, Changming, Wang, Lan, Ji, Qiushang, Li, Fajiu, Wang, Shengfeng, Zhang, Zhu, Zhang, Yunxia, Wang, Dingyi, Gao, Qian, Huang, Qiang, Xie, Wanmu, Zhai, Zhenguo, and Wang, Chen
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- 2024
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4. A simplified herbal decoction attenuates myocardial infarction by regulating macrophage metabolic reprogramming and phenotypic differentiation via modulation of the HIF-1α/PDK1 axis
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Lin, Zhi-jun, Dong, Xin, He, Huan, Jiang, Jia-lin, Guan, Zhuo-ji, Li, Xuan, Lu, Lu, Li, Huan, Huang, Yu-sheng, Xian, Shao-xiang, Yang, Zhong-qi, Chen, Zi-xin, Fang, Hong-cheng, and Wang, Ling-jun
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- 2024
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5. Small nodules (≤ 6 mm in diameter) of multiple primary lung cancers: prevalence and management
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Cheng, Hua, Li, Wen-hao, Li, Xiao-jian, Zhong, Hong-cheng, Wang, Xiao-jin, Lin, Yu-jing, Liu, Xue-guo, Wu, Xiang-wen, and Cao, Qing-dong
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- 2022
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6. Mucocele: a rare complication following stapled haemorrhoidopexy
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Wan, Xing-Yang, Fu, Yuan-Ji, Li, Gui-Ming, Xiao, Guo-Zhong, Guo, Zhi-Wei, Ren, Dong-Lin, Cao, Bo, and Lin, Hong-Cheng
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- 2022
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7. Non-Wilms' renal tumors in children: experience with 139 cases treated at a single center
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Fang, Yi Wei, Song, Hong Cheng, Sun, Ning, and Zhang, Wei Ping
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- 2022
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8. Bilateral versus unilateral balloon pulmonary angioplasty for inoperable chronic thromboembolic pulmonary hypertension
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Hong, Cheng, Lu, Jianmin, Wu, Xiaofeng, Guo, Wenliang, Lin, Jielong, Chen, Riken, Liu, Haimin, Chen, Haiming, Lei, Yongxia, Wang, Jian, Zhong, Yue, Zhuang, Chunying, and Wang, Xinlu
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- 2022
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9. Gastrointestinal dysfunction is associated with mortality in severe burn patients: a 10-year retrospective observational study from South China
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He, Qiu-Lan, Gao, Shao-Wei, Qin, Ying, Huang, Run-Cheng, Chen, Cai-Yun, Zhou, Fei, Lin, Hong-Cheng, and Huang, Wen-Qi
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- 2022
- Full Text
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10. Relationship between the invasion of lymphocytes and cytokines in the tumor microenvironment and the interval after single brachytherapy hypofractionated radiotherapy and conventional fractionation radiotherapy in non-small cell lung Cancer
- Author
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Li, Lin, Yue, Hong Cheng, Han, Yun Wei, Liu, Wei, Xiong, Liang Geng, and Zhang, Jian Wen
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- 2020
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11. Correction to: Efficacy and safety of tetramethylpyrazine phosphate on pulmonary hypertension: study protocol for a randomized controlled study
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Chen, Yuqin, He, Wenjun, Ouyang, Haiping, Liu, Chunli, Hong, Cheng, Wang, Tao, Yang, Kai, Lu, Wenju, and Wang, Jian
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- 2020
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12. Identification of a potentially functional circRNA–miRNA–mRNA regulatory network for investigating pathogenesis and providing possible biomarkers of bladder cancer
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Lu, Hong-cheng, Yao, Jia-qi, Yang, Xiao, Han, Jie, Wang, Jing-zi, Xu, Kun, Zhou, Rui, Yu, Hao, Lv, Qiang, and Gu, Min
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- 2020
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13. Efficacy and safety of tetramethylpyrazine phosphate on pulmonary hypertension: study protocol for a randomized controlled study
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Chen, Yuqin, He, Wenjun, Ouyang, Haiping, Liu, Chunli, Hong, Cheng, Wang, Tao, Yang, Kai, Lu, Wenju, and Wang, Jian
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- 2019
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14. METTL3 promote tumor proliferation of bladder cancer by accelerating pri-miR221/222 maturation in m6A-dependent manner
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Han, Jie, Wang, Jing-zi, Yang, Xiao, Yu, Hao, Zhou, Rui, Lu, Hong-Cheng, Yuan, Wen-Bo, Lu, Jian-chen, Zhou, Zi-jian, Lu, Qiang, Wei, Ji-Fu, and Yang, Haiwei
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- 2019
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15. Combined hepatocellular-cholangiocarcinoma: a population level analysis of incidence and mortality trends
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Wang, Jiakun, Li, Enliang, Yang, Hao, Wu, Junjun, Lu, Hong cheng, Yi, Chenhao, Lei, Jun, Liao, Wenjun, and Wu, Linquan
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- 2019
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16. Methylome and transcriptome analyses of apple fruit somatic mutations reveal the difference of red phenotype
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Jiang, Sheng-Hui, Sun, Qing-Guo, Chen, Min, Wang, Nan, Xu, Hai-Feng, Fang, Hong-Cheng, Wang, Yi-Cheng, Zhang, Zong-Ying, and Chen, Xue-Sen
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- 2019
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17. Two kinds of rare light chain cast nephropathy caused by multiple myeloma: case reports and literature review
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Lijun Sun, Hong Cheng, Guoqin Wang, Xiao-Yi Xu, Yi-pu Chen, and Hong-rui Dong
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Nephrology ,Male ,medicine.medical_specialty ,Pathology ,Urinalysis ,030232 urology & nephrology ,Renal function ,Case Report ,lcsh:RC870-923 ,Nephropathy ,03 medical and health sciences ,0302 clinical medicine ,Multiple myeloma ,Internal medicine ,medicine ,Humans ,Cast nephropathy ,Aged ,Light chain ,medicine.diagnostic_test ,Bortezomib ,business.industry ,Acute kidney injury ,Middle Aged ,medicine.disease ,lcsh:Diseases of the genitourinary system. Urology ,Crystalline casts ,030220 oncology & carcinogenesis ,Kidney Diseases ,Renal biopsy ,Amyloid casts ,business ,medicine.drug - Abstract
Background Light chain cast nephropathy (LCCN) is the most common renal disease caused by multiple myeloma (MM). In addition to ordinary light chain protein casts, there are a few rare casts with unique shapes, including light chain amyloid casts (LCAC) and light chain crystal casts (LCCC). Case presentations Here, we report two patients. Patient 1 is a 72-year-old man who was clinically diagnosed with MM and acute kidney injury (AKI). Pathological examination of a renal biopsy revealed that there were many amyloid casts in the distal tubules that had a lightly-stained central area and a deeply-stained burr-like edge. The marginal zone of the cast was positive for Congo red staining and contained numerous amyloid fibers, as observed by electron microscopy. No systemic amyloidosis was found. The patient received 4 courses of bortezomib-based chemotherapy, and then, his MM achieved partial remission. Patient 2 is a 57-year-old man who was also clinically diagnosed with MM and AKI. Pathological examination of a renal biopsy showed that there were many crystalline casts in the distal tubules that were fully or partially composed of crystals with different shapes, including rhomboid, needle, triangle, rectangle and other geometric shapes. Congo red staining was negative. Crystals were also detected in the urine of this patient. After 9 courses of treatment with a bortezomib-based regimen, his MM obtained complete remission and his renal function returned to normal. Conclusions LCAC and LCCC nephropathy caused by MM are two rare types of LCCN, and both have their own unique morphological manifestations. LCAC nephropathy may not be accompanied by systemic amyloidosis. The diagnosis of these two unique LCCNs must rely on renal biopsy pathology, and the discovery of urine crystals is of great significance for indicating LCCC nephropathy.
- Published
- 2021
18. Admission fasting plasma glucose is associated with in-hospital outcomes in patients with acute coronary syndrome and diabetes: findings from the improving Care for Cardiovascular Disease in China - Acute Coronary Syndrome (CCC-ACS) project
- Author
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Nan Ye, Lijiao Yang, Guoqin Wang, Weijing Bian, Fengbo Xu, Changsheng Ma, Dong Zhao, Jing Liu, Yongchen Hao, Jun Liu, Na Yang, Hong Cheng, and on behalf of the CCC-ACS
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Cardiovascular disease in China ,lcsh:Diseases of the circulatory (Cardiovascular) system ,medicine.medical_specialty ,Acute coronary syndrome ,endocrine system diseases ,Anemia ,030209 endocrinology & metabolism ,030204 cardiovascular system & hematology ,Stress hyperglycemia ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Diabetes mellitus ,medicine ,Adverse effect ,business.industry ,Diabetes ,Fasting plasma glucose ,nutritional and metabolic diseases ,Glycosylated hemoglobin ,medicine.disease ,Cardiac surgery ,lcsh:RC666-701 ,Heart failure ,Cardiology and Cardiovascular Medicine ,business ,Research Article - Abstract
Background The discrepancy between glycosylated hemoglobin (HbA1c) and fasting plasma glucose (FPG) in clinical practice may be related to factors such as acute stress, renal dysfunction, and anemia, and its relationship with in-hospital outcomes is uncertain. The aim of this study was to investigate the association between the type of discrepancy between HbA1c and FPG and in-hospital outcomes in patients with acute coronary syndrome (ACS) and diabetes. Methods The Improving Care for Cardiovascular Disease in China - Acute Coronary Syndrome (CCC-ACS) project is a national, hospital-based quality improvement project with an ongoing database. Patients with ACS, diabetes and complete HbA1c and FPG values at admission were included. The consistent group included patients with HbA1c 1c ≥ 6.5% and FPG ≥ 7.0 mmol/L. The discrepancy group included patients with HbA1c ≥ 6.5% and FPG 1c group) or HbA1c Results A total of 7762 patients were included in this study. The numbers of patients in the consistent and discrepancy groups were 5490 and 2272 respectively. In the discrepancy group, increased HbA1c accounted for 77.5% of discrepancies, and increased FPG accounted for 22.5% of discrepancies. After adjusting for confounders, patients in the increased FPG group had a 1.6-fold increased risk of heart failure (OR, 1.62; 95% CI, 1.08–2.44), a 1.6-fold increased risk of composite cardiovascular death and heart failure (OR, 1.63; 95% CI, 1.09–2.43), and a 1.6-fold increased risk of composite major adverse cardiovascular and cerebrovascular events (MACCEs) and heart failure (OR, 1.56; 95% CI, 1.08–2.24) compared to patients in the increased HbA1c group. Conclusions Patients with an increased FPG but normal HbA1c had a higher risk of in-hospital adverse outcomes than those with increased HbA1c but normal FPG. This result may indicate that when HbA1c and FPG are inconsistent in patients with ACS and diabetes, the increased FPG that may be caused by stress hyperglycemia may have a more substantial adverse effect than increased HbA1c, which may be caused by chronic hyperglycemia. These high-risk patients should be given more attention and closer monitoring in clinical practice. Trial registry Clinicaltrial.gov, NCT02306616. Registered 29 November 2014.
- Published
- 2020
19. High quality draft genome sequence of an extremely halophilic archaeon Natrinema altunense strain AJ2T
- Author
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Ying-Yi Huo, Hong Cheng, Jing Hu, Min Wu, and Xue-Wei Xu
- Subjects
0301 basic medicine ,Whole genome sequencing ,Genetics ,Rhodopsin ,biology ,Shotgun sequencing ,030106 microbiology ,Short Report ,Genome project ,biology.organism_classification ,Genome ,Halophilic archaea ,Salt lake ,Haloterrigena ,Natrinema ,03 medical and health sciences ,030104 developmental biology ,GenBank ,Light-driven pumps ,High-altitude ,GC-content - Abstract
Natrinema altunense strain AJ2T, a halophilic archaeal strain, was isolated from a high-altitude (3884 m) salt lake in Xinjiang, China. This strain requires at least 1.7 M NaCl to grow and can grow anaerobically in the presence of nitrate. To understand the genetics underlying its extreme phenotype, we de novo assembled the entire genome sequence of AJ2T (=CGMCC 1.3731T=JCM 12890T). We assembled 3,774,135 bp of a total of 4.4 Mb genome in only 20 contigs and noted its high GC content (64.6%). Subsequently we predicted the gene content and generated genome annotation to identify the relationship between the epigenetic characteristics and genomic features. The genome sequence contains 52 tRNA genes, 3 rRNA genes and 4,462 protein-coding genes, 3792 assigned as functional or hypothetical proteins in nr database. This Whole Genome Shotgun project was deposited in DDBJ/EMBL/GenBank under the accession JNCS00000000. We performed a Bayesian (Maximum-Likelihood) phylogenetic analysis using 16S rRNA sequence and obtained its relationship to other strains in the Natrinema and Haloterrigena genera. We also confirmed the ANI value between every two species of Natrinema and Haloterrigena genera. In conclusion, our analysis furthered our understanding of the extreme-environment adapted strain AJ2T by characterizing its genome structure, gene content and phylogenetic placement. Our detailed case study will contribute to our overall understanding of why Natrinema strains can survive in such a high-altitude salt lake.
- Published
- 2017
20. Prevalence and risk factors for cardiovascular disease among chronic kidney disease patients: results from the Chinese cohort study of chronic kidney disease (C-STRIDE)
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Si-Ping Han, Jun Yuan, Luxia Zhang, Ming-Hui Zhao, Jinwei Wang, Xin-Rong Zou, Hong Cheng, Xiao-Qin Wang, and Lan Wang
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Male ,Epidemiology ,030232 urology & nephrology ,030204 cardiovascular system & hematology ,Severity of Illness Index ,0302 clinical medicine ,Risk Factors ,Cardiovascular Disease ,Chronic Kidney Disease ,Odds Ratio ,Prevalence ,education.field_of_study ,Incidence (epidemiology) ,Age Factors ,Middle Aged ,C-STRIDE ,Nephrology ,Cardiovascular Diseases ,Creatinine ,Cohort ,Hypertension ,Female ,Cohort study ,Research Article ,Glomerular Filtration Rate ,Adult ,medicine.medical_specialty ,China ,Population ,Aortic Diseases ,03 medical and health sciences ,Asian People ,Diabetes mellitus ,Internal medicine ,medicine ,Diabetes Mellitus ,Humans ,Renal Insufficiency, Chronic ,education ,Vascular Calcification ,Cohort Study ,Aged ,business.industry ,Odds ratio ,medicine.disease ,Logistic Models ,Cerebrovascular Disease ,Multivariate Analysis ,business ,Kidney disease - Abstract
Background Although a high incidence of cardiovascular disease (CVD) is observed among chronic kidney disease (CKD) patients in developed countries, limited information is available about CVD prevalence and risk factors in the Chinese CKD population. The Chinese Cohort of Chronic Kidney Disease (C-STRIDE) was established to investigate the prevalence and risk factors of CVD among Chinese CKD patients. Methods Participants with stage 1–4 CKD (18–74 years of age) were recruited at 39 clinical centers located in 28 cities from 22 provinces of China. At entry, the socio-demographic status, medical history, anthropometric measurements and lifestyle behaviors were documented, and blood and urine samples were collected. Estimated glomerular filtration rate (eGFR) was calculated by the CKD-EPI creatinine equation. CVD diagnosis was based on patient self-report and review of medical records by trained staff. A multivariable logistic regression model was used to estimate the association between risk factors and CVD. Results Three thousand four hundred fifty-nine Chinese patients with pre-stage 5 CKD were enrolled, and 3168 finished all required examinations and were included in the study. In total, 40.8% of the cohort was female, with a mean age of 48.21 ± 13.70 years. The prevalence of CVD was 9.8%, and in 69.1% of the CVD cases cerebrovascular disease was observed. Multivariable analysis showed that increasing age, lower eGFR, presence of hypertension, abdominal aorta calcification and diabetes were associated with comorbid CVD among CKD patients. The odds ratios and 95% confidence intervals for these risk factors were 3.78 (2.55–5.59) for age 45–64 years and 6.07 (3.89–9.47) for age ≥65 years compared with age
- Published
- 2017
21. Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1-integrin signaling pathway in tumor cells
- Author
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Maoshan Chen, Zhenhua Li, Guanglun Yang, Haojun Luo, Hong Cheng, Gang Tu, Manran Liu, Weijie Fu, Jie Yuan, Qing Zhu, and Li Yang
- Subjects
Selective Estrogen Receptor Modulators ,Epithelial-Mesenchymal Transition ,Antineoplastic Agents, Hormonal ,Estrogen receptor ,Breast Neoplasms ,Biology ,Cell Line, Tumor ,medicine ,Humans ,Epithelial–mesenchymal transition ,skin and connective tissue diseases ,Extracellular Signal-Regulated MAP Kinases ,Estrogen receptor beta ,Uncategorized ,Medicine(all) ,Tumor microenvironment ,Integrin beta1 ,Fibroblasts ,Immunohistochemistry ,Fibronectins ,ErbB Receptors ,Tamoxifen ,src-Family Kinases ,Receptors, Estrogen ,Drug Resistance, Neoplasm ,Focal Adhesion Protein-Tyrosine Kinases ,Cancer cell ,Cancer research ,MCF-7 Cells ,Female ,Signal transduction ,GPER ,Biomarkers ,medicine.drug ,Research Article ,Signal Transduction - Abstract
Introduction Acquired tamoxifen resistance remains the major obstacle to breast cancer endocrine therapy. β1-integrin was identified as one of the target genes of G protein-coupled estrogen receptor (GPER), a novel estrogen receptor recognized as an initiator of tamoxifen resistance. Here, we investigated the role of β1-integrin in GPER-mediated tamoxifen resistance in breast cancer. Methods The expression of β1-integrin and biomarkers of epithelial-mesenchymal transition were evaluated immunohistochemically in 53 specimens of metastases and paired primary tumors. The function of β1-integrin was investigated in tamoxifen-resistant (MCF-7R) subclones, derived from parental MCF-7 cells, and MCF-7R β1-integrin-silenced subclones in MTT and Transwell assays. Involved signaling pathways were identified using specific inhibitors and Western blotting analysis. Results GPER, β1-integrin and mesenchymal biomarkers (vimentin and fibronectin) expression in metastases increased compared to the corresponding primary tumors; a close expression pattern of β1-integrin and GPER were in metastases. Increased β1-integrin expression was also confirmed in MCF-7R cells compared with MCF-7 cells. This upregulation of β1-integrin was induced by agonists of GPER and blocked by both antagonist and knockdown of it in MCF-7R cells. Moreover, the epidermal growth factor receptor/extracellular regulated protein kinase (EGFR/ERK) signaling pathway was involved in this transcriptional regulation since specific inhibitors of these kinases also reduced the GPER-induced upregulation of β1-integrin. Interestingly, silencing of β1-integrin partially rescued the sensitivity of MCF-7R cells to tamoxifen and the α5β1-integrin subunit is probably responsible for this phenomenon. Importantly, the cell migration and epithelial-mesenchymal transition induced by cancer-associated fibroblasts, or the product of cancer-associated fibroblasts, fibronectin, were reduced by knockdown of β1-integrin in MCF-7R cells. In addition, the downstream kinases of β1-integrin including focal adhesion kinase, Src and AKT were activated in MCF-7R cells and may be involved in the interaction between cancer cells and cancer-associated fibroblasts. Conclusions GPER/EGFR/ERK signaling upregulates β1-integrin expression and activates downstream kinases, which contributes to cancer-associated fibroblast-induced cell migration and epithelial-mesenchymal transition, in MCF-7R cells. GPER probably contributes to tamoxifen resistance via interaction with the tumor microenvironment in a β1-integrin-dependent pattern. Thus, β1-integrin may be a potential target to improve anti-hormone therapy responses in breast cancer patients. Electronic supplementary material The online version of this article (doi:10.1186/s13058-015-0579-y) contains supplementary material, which is available to authorized users.
- Published
- 2015
22. Complete genome sequence of esteraseproducing bacterium Croceicoccus marinus E4A9T.
- Author
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Yue-Hong Wu, Hong Cheng, Ying-Yi Huo, Lin Xu, Qian Liu, Chun-Sheng Wang, and Xue-Wei Xu
- Subjects
- *
ESTERASES , *BACTERIAL genomes , *NUCLEOTIDE sequence , *DEEP-sea ecology , *LAKE sediments , *PROTEOBACTERIA - Abstract
Croceicoccus marinus E4A9Twas isolated from deep-sea sediment collected from the East Pacific polymetallic nodule area. The strain is able to produce esterase, which is widely used in the food, perfume, cosmetic, chemical, agricultural and pharmaceutical industries. Here we describe the characteristics of strain E4A9, including the genome sequence and annotation, presence of esterases, and metabolic pathways of the organism. The genome of strain E4A9T comprises 4,109,188 bp, with one chromosome (3,001,363 bp) and two large circular plasmids (761,621 bp and 346,204 bp, respectively). Complete genome contains 3653 coding sequences, 48 tRNAs, two operons of 16S-23S-5S rRNA gene and three ncRNAs. Strain E4A9T encodes 10 genes related to esterase, and three of the esterases (E3, E6 and E10) was successfully cloned and expressed in Escherichia coli Rosetta in a soluble form, revealing its potential application in biotechnological industry. Moreover, the genome provides clues of metabolic pathways of strain E4A9T, reflecting its adaptations to the ambient environment. The genome sequence of C. marinus E4A9T now provides the fundamental information for future studies. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
23. Complete genome sequence of esteraseproducing bacterium Croceicoccus marinus E4A9T.
- Author
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Yue-Hong Wu, Hong Cheng, Ying-Yi Huo, Lin Xu, Qian Liu, Chun-Sheng Wang, and Xue-Wei Xu
- Subjects
ESTERASES ,BACTERIAL genomes ,NUCLEOTIDE sequence ,DEEP-sea ecology ,LAKE sediments ,PROTEOBACTERIA - Abstract
Croceicoccus marinus E4A9
T was isolated from deep-sea sediment collected from the East Pacific polymetallic nodule area. The strain is able to produce esterase, which is widely used in the food, perfume, cosmetic, chemical, agricultural and pharmaceutical industries. Here we describe the characteristics of strain E4A9, including the genome sequence and annotation, presence of esterases, and metabolic pathways of the organism. The genome of strain E4A9T comprises 4,109,188 bp, with one chromosome (3,001,363 bp) and two large circular plasmids (761,621 bp and 346,204 bp, respectively). Complete genome contains 3653 coding sequences, 48 tRNAs, two operons of 16S-23S-5S rRNA gene and three ncRNAs. Strain E4A9T encodes 10 genes related to esterase, and three of the esterases (E3, E6 and E10) was successfully cloned and expressed in Escherichia coli Rosetta in a soluble form, revealing its potential application in biotechnological industry. Moreover, the genome provides clues of metabolic pathways of strain E4A9T , reflecting its adaptations to the ambient environment. The genome sequence of C. marinus E4A9T now provides the fundamental information for future studies. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
24. WW domain-binding protein 2: an adaptor protein closely linked to the development of breast cancer.
- Author
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Shuai Chen, Han Wang, Yu-Fan Huang, Ming-Li Li, Jiang-Hong Cheng, Peng Hu, Chuan-Hui Lu, Ya Zhang, Na Liu, Chi-Meng Tzeng, and Zhi-Ming Zhan
- Subjects
CARRIER proteins ,ADAPTOR proteins ,BREAST cancer ,AMINO acids ,ESTROGEN receptors - Abstract
The WW domain is composed of 38 to 40 semi-conserved amino acids shared with structural, regulatory, and signaling proteins. WW domain-binding protein 2 (WBP2), as a binding partner of WW domain protein, interacts with several WW-domain-containing proteins, such as Yes kinase-associated protein (Yap), paired box gene 8 (Pax8), WW-domain-containing transcription regulator protein 1 (TAZ), and WW-domain-containing oxidoreductase (WWOX) through its PPxY motifs within C-terminal region, and further triggers the downstream signaling pathway in vitro and in vivo. Studies have confirmed that phosphorylated form of WBP2 can move into nuclei and activate the transcription of estrogen receptor (ER) and progesterone receptor (PR), whose expression were the indicators of breast cancer development, indicating that WBP2 may participate in the progression of breast cancer. Both overexpression of WBP2 and activation of tyrosine phosphorylation upregulate the signal cascades in the cross-regulation of the Wnt and ER signaling pathways in breast cancer. Following the binding of WBP2 to the WW domain region of TAZ which can accelerate migration, invasion and is required for the transformed phenotypes of breast cancer cells, the transformation of epithelial to mesenchymal of MCF10A is activated, suggesting that WBP2 is a key player in regulating cell migration. When WBP2 binds with WWOX, a tumor suppressor, ER transactivation and tumor growth can be suppressed. Thus, WBP2 may serve as a molecular on/off switch that controls the crosstalk between E2, WWOX, Wnt, TAZ, and other oncogenic signaling pathways. This review interprets the relationship between WBP2 and breast cancer, and provides comprehensive views about the function of WBP2 in the regulation of the pathogenesis of breast cancer and endocrine therapy in breast cancer treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
25. Complete genome sequence of bacteriochlorophyll-synthesizing bacterium Porphyrobacter neustonensis DSM 9434.
- Author
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Qian Liu, Yue-Hong Wu, Hong Cheng, Lin Xu, Chun-Sheng Wang, and Xue-Wei Xu
- Subjects
BACTERIOCHLOROPHYLLS ,NUCLEOTIDE sequencing ,RIBOSOMAL RNA ,TRANSFER RNA ,PHOTOSYNTHETIC bacteria - Abstract
The genus Porphyrobacter belongs to aerobic anoxygenic phototrophic bacteria cluster. Porphyrobacter neustonensis DSM 9434 was isolated from a eutrophic freshwater pond in Australia, and is able to synthesize Bacteriochlorophyll a as well as grow under aerobic conditions. It is the type species of the genus Porphyrobacter. Here we describe the characteristics of the strain DSM 9434, including the genome sequence and annotation, synthesis of BChl a, and metabolic pathways of the organism. The genome of strain DSM 9434 comprises 3,090,363 bp and contains 2,902 protein-coding genes, 47 tRNA genes and 6 rRNA genes. Strain DSM 9434 encodes 46 genes which participate in BChl a synthesis and this investigation shed light on the evolution and functional implications regarding bacteriochlorophyll synthesis. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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26. Application of hierarchical oligonucleotide primer extension (HOPE) to assess relative abundances of ammonia- and nitrite-oxidizing bacteria.
- Author
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Scarascia, Giantommaso, Hong Cheng, Harb, Moustapha, and Pei-Ying Hong
- Subjects
- *
MICROORGANISM populations , *AMMONIA-oxidizing bacteria , *NITRITES , *NITRIFICATION , *WATER purification - Abstract
Background: Establishing an optimal proportion of nitrifying microbial populations, including ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), complete nitrite oxidizers (comammox) and ammonia-oxidizing archaea (AOA), is important for ensuring the efficiency of nitrification in water treatment systems. Hierarchical oligonucleotide primer extension (HOPE), previously developed to rapidly quantify relative abundances of specific microbial groups of interest, was applied in this study to track the abundances of the important nitrifying bacterial populations. Results: The method was tested against biomass obtained from a laboratory-scale biofilm-based trickling reactor, and the findings were validated against those obtained by 16S rRNA gene-based amplicon sequencing. Our findings indicated a good correlation between the relative abundance of nitrifying bacterial populations obtained using both HOPE and amplicon sequencing. HOPE showed a significant increase in the relative abundance of AOB, specifically Nitrosomonas, with increasing ammonium content and shock loading (p < 0.001). In contrast, Nitrosospira remained stable in its relative abundance against the total community throughout the operational phases. There was a corresponding significant decrease in the relative abundance of NOB, specifically Nitrospira and those affiliated to comammox, during the shock loading. Based on the relative abundance of AOB and NOB (including commamox) obtained from HOPE, it was determined that the optimal ratio of AOB against NOB ranged from 0.2 to 2.5 during stable reactor performance. Conclusions: Overall, the HOPE method was developed and validated against 16S rRNA gene-based amplicon sequencing for the purpose of performing simultaneous monitoring of relative abundance of nitrifying populations. Quantitative measurements of these nitrifying populations obtained via HOPE would be indicative of reactor performance and nitrification functionality. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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- View/download PDF
27. High quality draft genome sequence of an extremely halophilic archaeon Natrinema altunense strain AJ2T.
- Author
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Hong Cheng, Ying-Yi Huo, Jing Hu, Xue-Wei Xu, and Min Wu
- Subjects
- *
NUCLEOTIDE sequencing , *ARCHAEBACTERIA , *HALOPHILIC microorganisms , *PHYLOGENY , *EPIGENETICS - Abstract
Natrinema altunense strain AJ2T, a halophilic archaeal strain, was isolated from a high-altitude (3884 m) salt lake in Xinjiang, China. This strain requires at least 1.7 M NaCl to grow and can grow anaerobically in the presence of nitrate. To understand the genetics underlying its extreme phenotype, we de novo assembled the entire genome sequence of AJ2T (=CGMCC 1.3731T=JCM 12890T). We assembled 3,774,135 bp of a total of 4.4 Mb genome in only 20 contigs and noted its high GC content (64.6%). Subsequently we predicted the gene content and generated genome annotation to identify the relationship between the epigenetic characteristics and genomic features. The genome sequence contains 52 tRNA genes, 3 rRNA genes and 4,462 protein-coding genes, 3792 assigned as functional or hypothetical proteins in nr database. This Whole Genome Shotgun project was deposited in DDBJ/EMBL/GenBank under the accession JNCS00000000. We performed a Bayesian (Maximum-Likelihood) phylogenetic analysis using 16S rRNA sequence and obtained its relationship to other strains in the Natrinema and Haloterrigena genera. We also confirmed the ANI value between every two species of Natrinema and Haloterrigena genera. In conclusion, our analysis furthered our understanding of the extreme-environment adapted strain AJ2T by characterizing its genome structure, gene content and phylogenetic placement. Our detailed case study will contribute to our overall understanding of why Natrinema strains can survive in such a high-altitude salt lake. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
28. High quality draft genome sequence of an extremely halophilic archaeon Natrinema altunense strain AJ2T.
- Author
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Hong Cheng, Ying-Yi Huo, Jing Hu, Xue-Wei Xu, and Min Wu
- Subjects
NUCLEOTIDE sequencing ,ARCHAEBACTERIA ,HALOPHILIC microorganisms ,PHYLOGENY ,EPIGENETICS - Abstract
Natrinema altunense strain AJ2
T , a halophilic archaeal strain, was isolated from a high-altitude (3884 m) salt lake in Xinjiang, China. This strain requires at least 1.7 M NaCl to grow and can grow anaerobically in the presence of nitrate. To understand the genetics underlying its extreme phenotype, we de novo assembled the entire genome sequence of AJ2T (=CGMCC 1.3731T =JCM 12890T ). We assembled 3,774,135 bp of a total of 4.4 Mb genome in only 20 contigs and noted its high GC content (64.6%). Subsequently we predicted the gene content and generated genome annotation to identify the relationship between the epigenetic characteristics and genomic features. The genome sequence contains 52 tRNA genes, 3 rRNA genes and 4,462 protein-coding genes, 3792 assigned as functional or hypothetical proteins in nr database. This Whole Genome Shotgun project was deposited in DDBJ/EMBL/GenBank under the accession JNCS00000000. We performed a Bayesian (Maximum-Likelihood) phylogenetic analysis using 16S rRNA sequence and obtained its relationship to other strains in the Natrinema and Haloterrigena genera. We also confirmed the ANI value between every two species of Natrinema and Haloterrigena genera. In conclusion, our analysis furthered our understanding of the extreme-environment adapted strain AJ2T by characterizing its genome structure, gene content and phylogenetic placement. Our detailed case study will contribute to our overall understanding of why Natrinema strains can survive in such a high-altitude salt lake. [ABSTRACT FROM AUTHOR]- Published
- 2017
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29. Prevalence and risk factors for cardiovascular disease among chronic kidney disease patients: results from the Chinese cohort study of chronic kidney disease (C-STRIDE).
- Author
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Jun Yuan, Xin-Rong Zou, Si-Ping Han, Hong Cheng, Lan Wang, Jin-Wei Wang, Lu-Xia Zhang, Ming-Hui Zhao, Xiao-Qin Wang, Yuan, Jun, Zou, Xin-Rong, Han, Si-Ping, Cheng, Hong, Wang, Lan, Wang, Jin-Wei, Zhang, Lu-Xia, Zhao, Ming-Hui, Wang, Xiao-Qin, and C-STRIDE study group
- Subjects
CHRONIC kidney failure ,CARDIOVASCULAR diseases ,CEREBROVASCULAR disease ,GLOMERULAR filtration rate ,EPIDEMIOLOGY ,HYPERTENSION ,PATIENTS ,HYPERTENSION epidemiology ,AGE distribution ,AORTIC diseases ,ASIANS ,CREATININE ,DIABETES ,MULTIVARIATE analysis ,LOGISTIC regression analysis ,DISEASE prevalence ,SEVERITY of illness index ,CALCINOSIS ,ODDS ratio - Abstract
Background: Although a high incidence of cardiovascular disease (CVD) is observed among chronic kidney disease (CKD) patients in developed countries, limited information is available about CVD prevalence and risk factors in the Chinese CKD population. The Chinese Cohort of Chronic Kidney Disease (C-STRIDE) was established to investigate the prevalence and risk factors of CVD among Chinese CKD patients.Methods: Participants with stage 1-4 CKD (18-74 years of age) were recruited at 39 clinical centers located in 28 cities from 22 provinces of China. At entry, the socio-demographic status, medical history, anthropometric measurements and lifestyle behaviors were documented, and blood and urine samples were collected. Estimated glomerular filtration rate (eGFR) was calculated by the CKD-EPI creatinine equation. CVD diagnosis was based on patient self-report and review of medical records by trained staff. A multivariable logistic regression model was used to estimate the association between risk factors and CVD.Results: Three thousand four hundred fifty-nine Chinese patients with pre-stage 5 CKD were enrolled, and 3168 finished all required examinations and were included in the study. In total, 40.8% of the cohort was female, with a mean age of 48.21 ± 13.70 years. The prevalence of CVD was 9.8%, and in 69.1% of the CVD cases cerebrovascular disease was observed. Multivariable analysis showed that increasing age, lower eGFR, presence of hypertension, abdominal aorta calcification and diabetes were associated with comorbid CVD among CKD patients. The odds ratios and 95% confidence intervals for these risk factors were 3.78 (2.55-5.59) for age 45-64 years and 6.07 (3.89-9.47) for age ≥65 years compared with age <45 years; 2.07 (1.28-3.34) for CKD stage 3a, 1.66 (1.00-2.62) for stage 3b, and 2.74 (1.72-4.36) for stage 4 compared with stages 1 and 2; 2.57 (1.50-4.41) for hypertension, 1.82 (1.23-2.70) for abdominal aorta calcification, and 1.70 (1.30-2.23) for diabetes, respectively.Conclusions: We reported the CVD prevalence among a CKD patient cohort and found age, hypertension, diabetes, abdominal aorta calcification and lower eGFR were independently associated with higher CVD prevalence. Prospective follow-up and longitudinal evaluations of CVD risk among CKD patients are warranted. [ABSTRACT FROM AUTHOR]- Published
- 2017
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30. High molecular weight of polysaccharides from Hericium erinaceus against amyloid beta-induced neurotoxicity.
- Author
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Jai-Hong Cheng, Chia-Ling Tsai, Yi-Yang Lien, Meng-Shiou Lee, and Shyang-Chwen Sheu
- Subjects
ANIMAL experimentation ,BIOLOGICAL transport ,CELL culture ,ENZYME-linked immunosorbent assay ,FLOW cytometry ,MICROSCOPY ,MITOCHONDRIA ,MUSHROOMS ,POLYSACCHARIDES ,RATS ,RESEARCH funding ,PLANT extracts ,NEUROPROTECTIVE agents ,DATA analysis software ,FREE radical scavengers ,DESCRIPTIVE statistics ,IN vitro studies ,ONE-way analysis of variance - Abstract
Background: Hericium erinaceus (HE) is a well-known mushroom in traditional Chinese food and medicine. HE extracts from the fruiting body and mycelia not only exhibit immunomodulatory, antimutagenic and antitumor activity but also have neuroprotective properties. Here, we purified HE polysaccharides (HEPS), composed of two high molecular weight polysaccharides (1.7 x 10
5 Da and 1.1 x 105 Da), and evaluated their protective effects on amyloid beta (Aβ)-induced neurotoxicity in rat pheochromocytoma PC12 cells. Methods: HEPS were prepared and purified using a 95 % ethanol extraction method. The components of HEPS were analyzed and the molecular weights of the polysaccharides were determined using high-pressure liquid chromatography (HPLC). The neuroprotective effects of the polysaccharides were evaluated through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and an MTT assay and by quantifying reactive oxygen species (ROS) and mitochondrial membrane potentials (MMP) of Aβ-induced neurotoxicity in cells. Result: Our results showed that 250 µg/ml HEPS was harmless and promoted cell viability with 1.2 µM Aβ treatment. We observed that the free radical scavenging rate exceeded 90 % when the concentration of HEPS was higher than 1 mg/mL in cells. The HEPS decreased the production of ROS from 80 to 58 % in a dose-dependent manner. Cell pretreatment with 250 µg/mL HEPS significantly reduced Aβ-induced high MMPs from 74 to 51 % and 94 to 62 % at 24 and 48 h, respectively. Finally, 250 µg/mL of HEPS prevented Aβ-induced cell shrinkage and nuclear degradation of PC1 2 cells. Conclusion: Our results demonstrate that HEPS exhibit antioxidant and neuroprotective effects on Aβ-induced neurotoxicity in neurons. . [ABSTRACT FROM AUTHOR]- Published
- 2016
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31. The association of CD40 polymorphisms with CD40 serum levels and risk of systemic lupus erythematosus.
- Author
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Jian-Ming Chen, Jing Guo, Chuan-Dong Wei, Chun-Fang Wang, Hong-Cheng Luo, Ye-Sheng Wei, and Yan Lan
- Subjects
LIGANDS (Biochemistry) ,SYSTEMIC lupus erythematosus ,SINGLE nucleotide polymorphisms ,GENE expression ,PUBLIC health - Abstract
Background: Current evidence shows that the CD40-CD40 ligand (CD40-CD40L) system plays a crucial role in the development, progression and outcome of systemic lupus erythematosus (SLE). The aim of this study was to investigate whether a CD40 gene single nucleotide polymorphism (SNP) is associated with SLE and CD40 expression in the Chinese population. We included controls (n = 220) and patients with either SLE (n =205) in the study. Methods: The gene polymorphism was measured using Snapshot SNP genotyping assays and confirmed by sequencing. We analyzed three single nucleotide polymorphisms of CD40 gene rs1883832C/T, rs1569723A/C and rs4810485G/T in 205 patients with SLE and 220 age-and sex-matched controls. Soluble CD40 (sCD40) levels were measured by ELISA. Results: There were significant differences in the genotype and allele frequencies of CD40 gene rs1883832C/T polymorphism between the group of patients with SLE and the control group (P < 0.05). sCD40 levels were increased in patients with SLE compared with controls (P < 0.01). Moreover, genotypes carrying the CD40 rs1883832 C/T variant allele were associated with increased CD40 levels compared to the homozygous wild-type genotype in patients with SLE. The rs1883832C/T polymorphism of CD40 and its sCD40 levels were associated with SLE in the Chinese population. Conclusions: Our results suggest that CD40 gene may play a role in the development of SLE in the Chinese population. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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32. Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1-integrin signaling pathway in tumor cells
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Jie Yuan, Manran Liu, Li Yang, Gang Tu, Qing Zhu, Maoshan Chen, Hong Cheng, Haojun Luo, Weijie Fu, Zhenhua Li, and Guanglun Yang
- Subjects
TAMOXIFEN ,BREAST cancer treatment ,CANCER cells ,G protein coupled receptors ,FIBRONECTINS ,IMMUNOHISTOCHEMISTRY - Abstract
Introduction: Acquired tamoxifen resistance remains the major obstacle to breast cancer endocrine therapy. β1-integrin was identified as one of the target genes of G protein-coupled estrogen receptor (GPER), a novel estrogen receptor recognized as an initiator of tamoxifen resistance. Here, we investigated the role of β1-integrin in GPER-mediated tamoxifen resistance in breast cancer. Methods: The expression of β1-integrin and biomarkers of epithelial-mesenchymal transition were evaluated immunohistochemically in 53 specimens of metastases and paired primary tumors. The function of β1-integrin was investigated in tamoxifen-resistant (MCF-7R) subclones, derived from parental MCF-7 cells, and MCF-7R β1-integrin-silenced subclones in MTT and Transwell assays. Involved signaling pathways were identified using specific inhibitors and Western blotting analysis. Results: GPER, β1-integrin and mesenchymal biomarkers (vimentin and fibronectin) expression in metastases increased compared to the corresponding primary tumors; a close expression pattern of β1-integrin and GPER were in metastases. Increased β1-integrin expression was also confirmed in MCF-7R cells compared with MCF-7 cells. This upregulation of β1-integrin was induced by agonists of GPER and blocked by both antagonist and knockdown of it in MCF-7R cells. Moreover, the epidermal growth factor receptor/extracellular regulated protein kinase (EGFR/ERK) signaling pathway was involved in this transcriptional regulation since specific inhibitors of these kinases also reduced the GPER-induced upregulation of β1-integrin. Interestingly, silencing of β1-integrin partially rescued the sensitivity of MCF-7R cells to tamoxifen and the α5β1-integrin subunit is probably responsible for this phenomenon. Importantly, the cell migration and epithelial-mesenchymal transition induced by cancer-associated fibroblasts, or the product of cancer-associated fibroblasts, fibronectin, were reduced by knockdown of β1-integrin in MCF-7R cells. In addition, the downstream kinases of β1-integrin including focal adhesion kinase, Src and AKT were activated in MCF-7R cells and may be involved in the interaction between cancer cells and cancer-associated fibroblasts. Conclusions: GPER/EGFR/ERK signaling upregulates β1-integrin expression and activates downstream kinases, which contributes to cancer-associated fibroblast-induced cell migration and epithelial-mesenchymal transition, in MCF-7R cells. GPER probably contributes to tamoxifen resistance via interaction with the tumor microenvironment in a β1-integrin-dependent pattern. Thus, β1-integrin may be a potential target to improve anti-hormone therapy responses in breast cancer patients. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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33. Platinum sensitivity and CD133 expression as risk and prognostic predictors of central nervous system metastases in patients with epithelial ovarian cancer.
- Author
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Bo-lin Liu, Shu-juan Liu, Baskys, Andrius, Hong Cheng, Ying Han, Chao Xie, Hui Song, Jia Li, and Xiao-yan Xin
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PHYSIOLOGICAL effects of platinum ,CD antigens ,GENE expression ,CENTRAL nervous system cancer ,METASTASIS ,OVARIAN cancer patients - Abstract
Background To characterize prognostic and risk factors of central nervous system (CNS) metastases in patients with epithelial ovarian cancer (EOC). Methods A retrospective analysis of Xijing Hospital electronic medical records was conducted to identify patients with pathologically confirmed EOC and CNS metastases. In addition to patient demographics, tumor pathology, treatment regimens, and clinical outcomes, we compared putative cancer stem cell marker CD133 expression patterns in primary and metastatic lesions as well as in recurrent EOC with and without CNS metastases. Results Among 1366 patients with EOC, metastatic CNS lesions were present in 29 (2.1%) cases. CD133 expression in primary tumor was the only independent risk factor for CNS metastases; whilst the extent of surgical resection of primary EOC and platinum resistance were two independent factors significantly associated with time to CNS metastases. Absence of CD133 expression in primary tumors was significantly associated with high platinum sensitivity in both patient groups with and without CNS metastases. Platinum resistance and CD133 cluster formation in CNS metastases were associated with decreased survival, while multimodal therapy including stereotactic radiosurgery (SRS) for CNS metastases was associated with increased survival following the diagnosis of CNS metastases. Conclusions These data suggest that there exist a positive association between CD133 expression in primary EOC, platinum resistance and the increased risk of CNS metastases, as well as a less favorable prognosis of EOC. The absence of CD133 clusters and use of multimodal therapy including SRS could improve the outcome of metastatic lesions. Further investigation is warranted to elucidate the true nature of the association between platinum sensitivity, CD133 expression, and the risk and prognosis of CNS metastases from EOC. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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34. Extracorporeal shockwave therapy in osteoporotic osteoarthritis of the knee in rats: an experiment in animals.
- Author
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Ching-Jen Wang, Chien-Yiu Huang, Shan-Ling Hsu, Jen-Hung Chen, and Jai-Hong Cheng
- Published
- 2014
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35. Insulin resistance determined by Homeostasis Model Assessment (HOMA) and associations with metabolic syndrome among Chinese children and teenagers.
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Jinhua Yin, Ming Li, Lu Xu, Ying Wang, Hong Cheng, Xiaoyuan Zhao, and Jie Mi
- Subjects
INSULIN resistance ,METABOLIC syndrome ,HOMEOSTASIS ,JUVENILE diseases ,DISEASES in teenagers - Abstract
Objective The aim of this study is to assess the association between the degree of insulin resistance and the different components of the metabolic syndrome among Chinese children and adolescents. Moreover, to determine the cut-off values for homeostasis model assessment of insulin resistance (HOMA-IR) at MS risk. Methods 3203 Chinese children aged 6 to 18 years were recruited. Anthropometric and biochemical parameters were measured. Metabolic syndrome (MS) was identified by a modified Adult Treatment Panel III (ATP III) definition. HOMA-IR index was calculated and the normal reference ranges were defined from the healthy participants. Receiver operating characteristic (ROC) analysis was used to find the optimal cutoff of HOMA-IR for diagnosis of MS. Results With the increase of insulin resistance (quintile of HOMA-IR value), the ORs of suffering MS or its related components were significantly increased. Participants in the highest quintile of HOMA-IR were about 60 times more likely to be classified with metabolic syndrome than those in the lowest quintile group. Similarly, the mean values of insulin and HOMA-IR increased with the number of MS components. The present HOMA-IR cutoff point corresponding to the 95th percentile of our healthy reference children was 3.0 for whole participants, 2.6 for children in prepubertal stage and 3.2 in pubertal period, respectively. The optimal point for diagnosis of MS was 2.3 in total participants, 1.7 in prepubertal children and 2.6 in pubertal adolescents, respectively, by ROC curve, which yielded high sensitivity and moderate specificity for a screening test. According to HOMA-IR > 3.0, the prevalence of insulin resistance in obese or MS children were 44.3% and 61.6% respectively. Conclusions Our data indicates insulin resistance is common among Chinese obese children and adolescents, and is strongly related to MS risk, therefore requiring consideration early in life. As a reliable measure of insulin resistance and assessment of MS risk, the optimal HOMA-IR cut-off points in this cohort were developed with variation regarding puberty. HOMA-IR may be useful for early evaluating insulin resistance in children and teenagers and could have a long-term benefit of preventive and diagnostic therapeutic intervention. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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36. Prevalence of metabolic syndrome in a cohort of Chinese schoolchildren: comparison of two definitions and assessment of adipokines as components by factor analysis.
- Author
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Qiaoxuan Wang, Jinhua Yin, Lu Xu, Hong Cheng, Xiaoyuan Zhao, Hongding Xiang, Lam, Hugh Simon, Jie Mi, and Ming Li
- Subjects
METABOLIC syndrome ,SCHOOL children ,ADIPOKINES ,CHINESE people ,FACTOR analysis ,COMPARATIVE studies ,COHORT analysis ,PREVENTION ,DISEASES ,JUVENILE diseases - Abstract
Background: Although attention to metabolic syndrome (MetS) in children has increased, there is still no universally accepted definition and its pathogenesis remains unclear. Our aim was to compare the current definitions of childhood MetS in a Chinese cohort and to examine the clustering pattern of MetS risk factors, particularly inclusion of leptin and adiponectin as additional components. Methods: 3373 schoolchildren aged 6 to 18 years were recruited. Anthropometric and biochemical parameters and adipokines were measured. MetS was identified using both the International Diabetes Federation (IDF) and a modified Adult Treatment Panel III (ATP III) definitions. Exploratory factor analysis was performed to establish grouping of metabolic characteristics. Results: For children =10 years, the prevalence of MetS was 14.3% in the obese group and 3.7% in the overweight group according to the new IDF definition, and 32.3% in the obese group and 8.4% in the overweight group according to the modified ATPIII definition. Frequency of hypertriglyceridemia, low high-density lipoprotein cholesterol (HDL-C), impaired fasting glucose, elevated blood pressure, and central obesity according to the new IDF definition was 16.7%, 20.7%, 15.8%, 25.5% and 75.5% in obese boys and 14.7%, 24.0%, 12.0%, 11.0% and 89.0% in obese girls, respectively. Metabolic abnormalities in children under 10 years of age were also noted. Using factor analysis on eight conventional variables led to the extraction of 3 factors. Waist circumference (WC) provided a connection between two factors in boys and all three factors in girls, suggesting its central role in the clustering of metabolic risk factors. Addition of leptin and adiponectin also led to the extraction of 3 factors, with leptin providing a connection between two factors in girls. When using WC, mean arterial pressure, triglyceride/HDL-C ratio, HOMA-IR and leptin/adiponectin ratio as variables, a single-factor model was extracted. WC had the biggest factor loading, followed by leptin/adiponectin ratio. Conclusions: MetS was highly prevalent amongst obese children and adolescents in this cohort, regardless of the definition used. Central obesity is the key player in the clustering of metabolic risk factors in children, supporting the new IDF definition. Moreover, our findings suggest that a common factor may underlie MetS. Leptin/ adiponectin ratio as a possible component of MetS deserves further consideration. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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37. Quantitative criteria for improving performance of buccal DNA for high-throughput genetic analysis.
- Author
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Woo, Jessica G., Martin, Lisa J., Ding, Lili, Brown, W. Mark, Howard, Timothy D., Langefeld, Carl D., Moomaw, Charles J., Haverbusch, Mary, Guangyun Sun, Indugula, Subba R., Hong Cheng, Deka, Ranjan, and Woo, Daniel
- Subjects
DNA ,GENES ,ALLELES ,HETEROZYGOSITY ,BUCCAL administration - Abstract
Background: DNA from buccal brush samples is being used for high-throughput analyses in a variety of applications, but the impact of sample type on genotyping success and downstream statistical analysis remains unclear. The objective of the current study was to determine laboratory predictors of genotyping failure among buccal DNA samples, and to evaluate the successfully genotyped results with respect to analytic quality control metrics. Sample and genotyping characteristics were compared between buccal and blood samples collected in the population-based Genetic and Environmental Risk Factors for Hemorrhagic Stroke (GERFHS) study (https:// gerfhs.phs.wfubmc.edu/public/index.cfm). Results: Seven-hundred eight (708) buccal and 142 blood DNA samples were analyzed for laboratory-based and analysis metrics. Overall genotyping failure rates were not statistically different between buccal (11.3%) and blood (7.0%, p = 0.18) samples; however, both the Contrast Quality Control (cQC) rate and the dynamic model (DM) call rates were lower among buccal DNA samples (p < 0.0001). The ratio of double-stranded to total DNA (ds/total ratio) in the buccal samples was the only laboratory characteristic predicting sample success (p < 0.0001). A threshold of at least 34% ds/total DNA provided specificity of 98.7% with a 90.5% negative predictive value for eliminating probable failures. After genotyping, median sample call rates (99.1% vs. 99.4%, p < 0.0001) and heterozygosity rates (25.6% vs. 25.7%, p = 0.006) were lower for buccal versus blood DNA samples, respectively, but absolute differences were small. Minor allele frequency differences from HapMap were smaller for buccal than blood samples, and both sample types demonstrated tight genotyping clusters, even for rare alleles. Conclusions: We identified a buccal sample characteristic, a ratio of ds/total DNA <34%, which distinguished buccal DNA samples likely to fail high-throughput genotyping. Applying this threshold, the quality of final genotyping resulting from buccal samples is somewhat lower, but compares favorably to blood. Caution is warranted if cases and controls have different sample types, but buccal samples provide comparable results to blood samples in large-scale genotyping analyses. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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38. The common rs9939609 variant of the fat massand obesity-associated gene is associated withobesity risk in children and adolescents ofBeijing, China.
- Author
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Bo Xi, Yue Shen, Meixian Zhang, Xin Liu, Xiaoyuan Zhao, Lijun Wu, Hong Cheng, Dongqing Hou, Lindpaintner, Klaus, Lisheng Liu, Jie Mi, and Xingyu Wang
- Subjects
OBESITY ,TYPE 2 diabetes ,BODY mass index ,GENETICS of disease susceptibility - Abstract
Background: Previous genome-wide association studies for type 2 diabetes susceptibility genes have confirmed that a common variant, rs9939609, in the fat mass and obesity associated (FTO) gene region is associated with body mass index (BMI) in European children and adults. A significant association of the same risk allele has been described in Asian adult populations, but the results are conflicting. In addition, no replication studies have been conducted in children and adolescents of Asian ancestry. Methods: A population-based survey was carried out among 3503 children and adolescents (6-18 years of age) in Beijing, China, including 1229 obese and 2274 non-obese subjects. We investigated the association of rs9939609 with BMI and the risk of obesity. In addition, we tested the association of rs9939609 with weight, height, waist circumference, waist-to-height ratio, fat mass percentage, birth weight, blood pressure and related metabolic traits. Results: We found significant associations of rs9939609 variant with weight, BMI, BMI standard deviation score (BMI-SDS), waist circumference, waist-to-height ratio, and fat mass percentage in children and adolescents (p for trend = 3.29 × 10
-5 , 1.39 × 10-6 , 3.76 × 10-6 , 2.26 × 10-5 , 1.94 × 10-5 , and 9.75 × 10-5 , respectively). No significant associations were detected with height, birth weight, systolic and diastolic blood pressure and related metabolic traits such as total cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol and fasting plasma glucose (all p > 0.05). Each additional copy of the rs9939609 A allele was associated with a BMI increase of 0.79 [95% Confidence interval (CI) 0.47 to 1.10] kg/m2, equivalent to 0.25 (95%CI 0.14 to 0.35) BMI-SDS units. This rs9939609 variant is significantly associated with the risk of obesity under an additive model [Odds ratio (OR) = 1.29, 95% CI 1.11 to 1.50] after adjusting for age and gender. Moreover, an interaction between the FTO rs9939609 genotype and physical activity (p < 0.001) was detected on BMI levels, the effect of rs9939609-A allele on BMI being (0.95 ± 0.10), (0.77 ± 0.08) and (0.67 ± 0.05) kg/m2, for subjects who performed low, moderate and severe intensity physical activity. Conclusion: The FTO rs9939609 variant is strongly associated with BMI and the risk of obesity in a population of children and adolescents in Beijing, China. [ABSTRACT FROM AUTHOR]- Published
- 2010
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- View/download PDF
39. Id l induces apoptosis through inhibition of RORgammat expression.
- Author
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Yuanzheng Yang, Hong-Cheng Wang, and Xiao-Hong Sun
- Subjects
- *
TRANSCRIPTION factors , *T cells , *LYMPHOCYTES , *PROTEINS , *MESSENGER RNA - Abstract
Background: Basic helix-loop-helix E proteins are transcription factors that play crucial roles in T cell development by controlling thymocyte proliferation, differentiation and survival. E protein functions can be repressed by their naturally occurring inhibitors, Id proteins (Id1-4). Transgenic expression of Id1 blocks T cell development and causes massive apoptosis of developing thymocytes. However, the underlying mechanisms are not entirely understood due to relatively little knowledge of the target genes regulated by E proteins. Results: We designed a unique strategy to search for genes directly controlled by E proteins and found RORγt to be a top candidate. Using microarray analyses and reverse-transcriptase PCR assays, we showed that Id1 expression diminished RORγt mRNA levels in T cell lines and primary thymocytes while induction of E protein activity restored RORγt expression. E proteins were found to specifically bind to the promoter region of RORγt, suggesting their role in activating transcription of the gene. Functional significance of E protein-controlled RORγt expression was established based on the finding that RORγt rescued apoptosis caused by Id1 overexpression. Furthermore, expression of RORγt prevented Id1-induced p38 MAP kinase hyper-activation. Conclusion: These results suggest that E protein-dependent RORγt gene expression aids the survival of developing thymocytes, which provides a possible explanation for the massive apoptosis found in Id1 transgenic mice. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
40. The association of CD40 polymorphisms with CD40 serum levels and risk of systemic lupus erythematosus.
- Author
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Chen JM, Guo J, Wei CD, Wang CF, Luo HC, Wei YS, and Lan Y
- Subjects
- Adult, Aged, Aged, 80 and over, Case-Control Studies, Female, Gene Frequency genetics, Haplotypes genetics, Humans, Male, Middle Aged, Risk Factors, CD40 Antigens blood, CD40 Antigens genetics, Genetic Association Studies, Genetic Predisposition to Disease, Lupus Erythematosus, Systemic blood, Lupus Erythematosus, Systemic genetics, Polymorphism, Single Nucleotide genetics
- Abstract
Background: Current evidence shows that the CD40-CD40 ligand (CD40-CD40L) system plays a crucial role in the development, progression and outcome of systemic lupus erythematosus (SLE). The aim of this study was to investigate whether a CD40 gene single nucleotide polymorphism (SNP) is associated with SLE and CD40 expression in the Chinese population. We included controls (n = 220) and patients with either SLE (n =205) in the study., Methods: The gene polymorphism was measured using Snapshot SNP genotyping assays and confirmed by sequencing. We analyzed three single nucleotide polymorphisms of CD40 gene rs1883832C/T, rs1569723A/C and rs4810485G/T in 205 patients with SLE and 220 age-and sex-matched controls. Soluble CD40 (sCD40) levels were measured by ELISA., Results: There were significant differences in the genotype and allele frequencies of CD40 gene rs1883832C/T polymorphism between the group of patients with SLE and the control group (P < 0.05). sCD40 levels were increased in patients with SLE compared with controls (P < 0.01). Moreover, genotypes carrying the CD40 rs1883832 C/T variant allele were associated with increased CD40 levels compared to the homozygous wild-type genotype in patients with SLE. The rs1883832C/T polymorphism of CD40 and its sCD40 levels were associated with SLE in the Chinese population., Conclusions: Our results suggest that CD40 gene may play a role in the development of SLE in the Chinese population.
- Published
- 2015
- Full Text
- View/download PDF
41. Id1 induces apoptosis through inhibition of RORgammat expression.
- Author
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Yang Y, Wang HC, and Sun XH
- Subjects
- Animals, Cell Differentiation drug effects, Cell Line, Gene Expression Regulation, Mice, Nuclear Receptor Subfamily 1, Group F, Member 3, Oligonucleotide Array Sequence Analysis, Receptors, Estrogen genetics, Receptors, Estrogen metabolism, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, T-Lymphocytes metabolism, TCF Transcription Factors genetics, TCF Transcription Factors metabolism, Tamoxifen pharmacology, Transcription Factor 7-Like 1 Protein, Transduction, Genetic, Apoptosis drug effects, Inhibitor of Differentiation Protein 1 genetics, Inhibitor of Differentiation Protein 1 metabolism, Receptors, Retinoic Acid biosynthesis, Receptors, Retinoic Acid metabolism, Receptors, Thyroid Hormone biosynthesis, Receptors, Thyroid Hormone metabolism
- Abstract
Background: Basic helix-loop-helix E proteins are transcription factors that play crucial roles in T cell development by controlling thymocyte proliferation, differentiation and survival. E protein functions can be repressed by their naturally occurring inhibitors, Id proteins (Id1-4). Transgenic expression of Id1 blocks T cell development and causes massive apoptosis of developing thymocytes. However, the underlying mechanisms are not entirely understood due to relatively little knowledge of the target genes regulated by E proteins., Results: We designed a unique strategy to search for genes directly controlled by E proteins and found RORgammat to be a top candidate. Using microarray analyses and reverse-transcriptase PCR assays, we showed that Id1 expression diminished RORgammat mRNA levels in T cell lines and primary thymocytes while induction of E protein activity restored RORgammat expression. E proteins were found to specifically bind to the promoter region of RORgammat, suggesting their role in activating transcription of the gene. Functional significance of E protein-controlled RORgammat expression was established based on the finding that RORgammat rescued apoptosis caused by Id1 overexpression. Furthermore, expression of RORgammat prevented Id1-induced p38 MAP kinase hyper-activation., Conclusion: These results suggest that E protein-dependent RORgammat gene expression aids the survival of developing thymocytes, which provides a possible explanation for the massive apoptosis found in Id1 transgenic mice.
- Published
- 2008
- Full Text
- View/download PDF
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