97 results on '"Hong Xu"'
Search Results
2. Effect of mobile-based cognitive behavior therapy (CBT) on lowering of blood lipid levels in atherosclerotic cardiovascular disease (ASCVD) patients: study protocol for a multicenter, prospective, randomized controlled trial
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Hong, Xu-Lin, Luan, Yi, Liu, Hong-Ying, and Zhang, Wen-Bin
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- 2022
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3. GCH1 variants contribute to the risk and earlier age-at-onset of Parkinson’s disease: a two-cohort case-control study
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Pan, Hong-xu, Zhao, Yu-wen, Mei, Jun-pu, Fang, Zheng-huan, Wang, Yige, Zhou, Xun, Zhou, Yang-jie, Zhang, Rui, Zhang, Kai-lin, Jiang, Li, Zeng, Qian, He, Yan, Wang, Zheng, Liu, Zhen-hua, Xu, Qian, Sun, Qi-ying, Yang, Yang, Hu, Ya-cen, Chen, Ya-se, Du, Juan, Lei, Li-fang, Zhang, Hai-nan, Wang, Chun-yu, Yan, Xin-xiang, Shen, Lu, Jiang, Hong, Tan, Jie-qiong, Li, Jin-chen, Tang, Bei-sha, and Guo, Ji-feng
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- 2020
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4. RMalign: an RNA structural alignment tool based on a novel scoring function RMscore
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Zheng, Jinfang, Xie, Juan, Hong, Xu, and Liu, Shiyong
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- 2019
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5. Risk factors of opioid use associated with an enhanced-recovery programme after total knee arthroplasty
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Guorui Cao, Shiqi Xiang, Minglu Yang, Songtao Quan, Junna Yao, Litao Cai, Wei Feng, Xiuli Yang, Hong Xu, Zeyu Huang, Shaoyun Zhang, Chen Yue, Honglue Tan, and Fuxing Pei
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Analgesics, Opioid ,Rheumatology ,RC925-935 ,Risk factors ,Total knee arthroplasty ,Length of stay ,Humans ,Orthopedics and Sports Medicine ,Diseases of the musculoskeletal system ,Opioid ,Arthroplasty, Replacement, Knee ,Research Article - Abstract
Background Characterizing the impacts of postoperative opioid use on total knee arthroplasty (TKA) patients may help optimize the pain management after TKA. The aim of the study is to examine the prevalence and risk factors for opioid use with an enhanced-recovery programme after primary TKA. Methods We identified 361 patients undergoing TKA, and separated those on the basis of whether to receive opioid use after surgery. Themultivariate logistic regression model was used to identify independent risk factors for opioid use after primary TKA. Length of stay (LOS) and postoperative complications were also recorded and compared. Results The prevalence of opioid use after primary TKA was 23.0%. The significant risk factor was the longer operative time (OR [odds ratio] = 1.017, 95% CI [confidence interval] = 1.001 to 1.032, p = 0.034) and the protective factor was the utilization of tranexamic acid(OR= 0.355, 95% CI = 0.161 to 0.780, p = 0.010). In addition, the LOS was longer in opioid group (p < 0.05). Conclusion Considering the adverse health effects of opioid use, strategies need to be developed to prevent persistent opioid use after TKA. Reducing operative time and the application of tranexamic acid could lower the risk of opioid use with an enhanced-recovery programme after primary TKA.
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- 2021
6. The association of antidiabetic medications and Mini-Mental State Examination scores in patients with diabetes and dementia
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Michael Alvarsson, Hong Xu, Sara Garcia-Ptacek, Niklas Hammar, Bengt Winblad, Dorota Religa, Maria Eriksdotter, Emilia Schwertner, and Juraj Secnik
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medicine.medical_specialty ,medicine.drug_class ,Cognitive Neuroscience ,Neurosciences. Biological psychiatry. Neuropsychiatry ,Type 2 diabetes ,DPP-4i ,MMSE ,Diabetes mellitus ,Internal medicine ,Antidiabetics ,medicine ,Dementia ,Humans ,Hypoglycemic Agents ,Cognitive decline ,RC346-429 ,Dipeptidyl-Peptidase IV Inhibitors ,Mini–Mental State Examination ,medicine.diagnostic_test ,business.industry ,Research ,Diabetes ,medicine.disease ,Sulfonylurea ,Confidence interval ,Metformin ,Sulfonylurea Compounds ,Neurology ,Diabetes Mellitus, Type 2 ,Neurology. Diseases of the nervous system ,Neurology (clinical) ,business ,RC321-571 ,medicine.drug - Abstract
Background The effect of antidiabetic medication on cognitive function is unclear. We analyzed the association between five antidiabetic drugs and change in Mini-Mental State Examination (MMSE) scores in patients with diabetes and dementia. Methods Using the Swedish Dementia Registry and four supplementary Swedish registers/databases, we identified 1873 patients (4732 observations) with diagnosis of type 2 diabetes (diabetes) and Alzheimer’s disease or mixed-pathology dementia who were followed up at least once after dementia diagnosis. Use of metformin, insulin, sulfonylurea, thiazolidinediones (TZD), and dipeptidyl-peptidase-4 inhibitors (DPP-4i) was identified at baseline. Prevalent-user, incident-user, and drug-drug cohorts were sampled, and propensity-score matching was used to analyze comparable subjects. Beta coefficients with 95% confidence intervals (CI) from the random intercept and slope linear mixed-effects models determined the association between the use of antidiabetic medications and decline in MMSE score points between the follow-ups. Inverse-probability weighting was used to account for patient dropout. Results Compared to non-users, prevalent users of metformin (beta 0.89, 95% CI 0.44; 1.33) and DPP-4i (0.72, 0.06; 1.37) experienced a slower cognitive decline with time. Secondly, compared to DPP-4i, the use of insulin (−1.00, −1.95; −0.04) and sulfonylureas (−1.19; −2.33; −0.04) was associated with larger point-wise decrements in MMSE with annual intervals. Conclusions In this large cohort of patients with diabetes and dementia, the use of metformin and DPP-4i was associated with a slower decline in MMSE scores. Further examination of the cognitive effects of metformin and incretin-based medications is warranted.
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- 2021
7. Robotic arm-assisted total knee arthroplasty improves preoperative planning and intraoperative decision-making
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Xufeng Wan, Zongke Zhou, Hong Xu, Duan Wang, Qiang Su, Mingcheng Yuan, and Yahao Lai
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musculoskeletal diseases ,medicine.medical_specialty ,Knee Joint ,Total knee arthroplasty ,Preoperative TKA planning ,Osteoarthritis ,Diseases of the musculoskeletal system ,Robotic Surgical Procedures ,medicine ,Humans ,Robot-assisted surgery ,Orthopedics and Sports Medicine ,Femur ,Tibia ,Fibula ,Arthroplasty, Replacement, Knee ,Orthopedic surgery ,Preoperative planning ,business.industry ,TKA implant sizing ,Reproducibility of Results ,Osteoarthritis, Knee ,medicine.disease ,musculoskeletal system ,RC925-935 ,Surgery ,business ,Nuclear medicine ,Knee Prosthesis ,Robotic arm ,RD701-811 ,Research Article - Abstract
Background The reliability of robotic arm-assisted total knee arthroplasty (RA-TKA) has been previously reported. In this study, we evaluated the predictive accuracy of the RA-TKA system in determining the required bone resection and implant size preoperatively and its effect on intraoperative decision-making. Methods Data on the outcomes of RA-TKA procedures performed in our department were prospectively collected. A three-dimensional model of the femur, tibia, and fibula was reconstructed using standard computed tomography (CT) images. The model was used preoperatively to predict bone required resection for the femur and tibia and implant size. Intraoperatively, the images were registered to the local anatomy to create a patient-specific model for decision-making, including real-time measurement of the medial-to-lateral difference in the extension/flexion gap and TKA component alignment. Differences between predicted and real bone resections and implant size were evaluated, and the post-TKA mechanical axis of the lower limb and difference in medial-to-lateral flexion/extension gap were measured. Results The analysis was based on the data of 28 patients who underwent TKA to treat severe osteoarthritis. The RA-TKA system successfully predicted the femoral and tibial component within one implant size in 28/28 cases (100%). For the 168 bone resections performed, including both femoral and tibial cuts, the resection was within 1 mm of the predicted value in 120/168 (71%) of the cuts. The actual versus predicted bone resection was statistically different only for the lateral tibial plateau (p = 0.018). The medial-to-lateral gap difference was between − 1 and 1 mm, except in one case. The achieved lower limb alignment was accurate overall, with the alignment being within Conclusions The RA-TKA system provided considerable pre- and intraoperative surgical assistance to achieve accurate bone resection, appropriate component sizing, and postoperative alignment after RA-TKA.
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- 2021
8. Plasma levels of D-dimer and fibrin degradation product are unreliable for diagnosing periprosthetic joint infection in patients undergoing re-revision arthroplasty
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Zongke Zhou, Zeyu Huang, Duan Wang, Jinwei Xie, Qiang Huang, and Hong Xu
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medicine.medical_specialty ,Prosthesis-Related Infections ,medicine.medical_treatment ,Arthroplasty, Replacement, Hip ,Youden's J statistic ,Fibrin degradation product ,Periprosthetic ,Diseases of the musculoskeletal system ,Blood Sedimentation ,Gastroenterology ,Fibrin Fibrinogen Degradation Products ,Plasma ,Internal medicine ,D-dimer ,Diagnosis ,medicine ,Periprosthetic joint infection ,Humans ,Orthopedics and Sports Medicine ,Retrospective Studies ,Orthopedic surgery ,Arthritis, Infectious ,medicine.diagnostic_test ,Receiver operating characteristic ,business.industry ,Interleukin-6 ,Arthroplasty ,C-Reactive Protein ,RC925-935 ,Erythrocyte sedimentation rate ,Surgery ,Re-revision arthroplasty ,business ,RD701-811 ,Biomarkers ,Research Article - Abstract
Background The preoperative diagnosis of periprosthetic joint infection (PJI) in patients undergoing re-revision arthroplasty is crucial, so we evaluated whether plasma levels of D-dimer and fibrin degradation product (FDP) could aid such diagnosis. Methods We retrospectively analyzed data on patients who underwent re-revision hip or knee arthroplasty at our institute during 2008–2020. Patients were stratified into those who experienced PJI or not, based on 2013 International Consensus Meeting Criteria. Plasma levels of D-dimer and FDP as well as levels of the traditional inflammatory biomarkers C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and interleukin-6 were compared between the groups. The ability of these biomarkers to diagnose PJI was assessed based on the area under the receiver operating characteristic (AUC) curve, for which predictive cut-offs were optimized based on the Youden index. Results Based on a cut-off of 0.80 mg/L, D-dimer gave an AUC of 0.595, high sensitivity of 85.7% but poor specificity of 47.8%. Based on a cut-off of 2.80 mg/L, FDP gave an AUC of 0.550, poor sensitivity of 56.5% and poor specificity of 52.9%. CRP, ESR and interleukin-6 showed much better diagnostic ability, with AUCs > 0.82. The combination of CRP and interleukin-6 gave an AUC of 0.877, high sensitivity of 91.7% and acceptable specificity of 78.3%. Conclusions Plasma levels of D-dimer and FDP may be inappropriate for diagnosing PJI in patients undergoing re-revision arthroplasty, whereas the combination of serum CRP and interleukin-6 may be effective.
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- 2021
9. Effects of hormone replacement therapy on glucose and lipid metabolism in peri- and postmenopausal women with a history of menstrual disorders
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Peiqiong Chen, Yang Song, Tongyun Qi, Linjuan Ma, Yizhou Huang, Wenxian Xu, Ketan Chu, Ling Xu, Hong Xu, Jianhong Zhou, Yibing Lan, Jiehong Zheng, Yuqun Cai, Saisai Li, and Chunming Li
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Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,media_common.quotation_subject ,Physiology ,Peri- and postmenopausal women ,Diseases of the endocrine glands. Clinical endocrinology ,03 medical and health sciences ,Glucose and lipid metabolism ,0302 clinical medicine ,Insulin resistance ,Diabetes mellitus ,medicine ,Humans ,Glucose homeostasis ,030212 general & internal medicine ,Prospective cohort study ,Menstruation Disturbances ,Menstrual cycle ,Retrospective Studies ,media_common ,030219 obstetrics & reproductive medicine ,business.industry ,Hormone replacement therapy (menopause) ,Lipid metabolism ,General Medicine ,Middle Aged ,RC648-665 ,Lipid Metabolism ,Prognosis ,medicine.disease ,Polycystic ovary ,Postmenopause ,Menstrual disorders ,Glucose ,Hormone replacement therapy ,Case-Control Studies ,Female ,business ,Biomarkers ,Follow-Up Studies ,Research Article - Abstract
Background Previous studies have indicated that women with a history of menstrual disorders have an increased risk of metabolic and cardiovascular diseases. This has been attributed to the high proportion of polycystic ovary syndrome (PCOS) among this group. The favorable effects of hormone replacement therapy (HRT) on serum lipid profiles and glucose homeostasis in postmenopausal women is widely accepted. Whether HRT can also show positive effects on metabolic homeostasis in menopausal women with prior menstrual disorders (a putative PCOS phenotype) has not been reported yet. The aim of the study was to compare the effects of HRT on glucose and lipid metabolism in peri- and postmenopausal women with prior menstrual disorders and controls who did not have prior menstrual disorders. Methods A retrospective multicenter study was conducted including 595 peri- and postmenopausal women who received HRT at four hospitals in the Zhejiang Province from May 31, 2010 to March 8, 2021. Participants were divided into the Normal menstruation group and the Menstrual disorders group according to their prior usual menstrual cycle pattern. Glucose and lipid metabolism indicators were assessed at baseline and after HRT. The results were compared between and within the groups, and data from peri- and postmenopausal women were analyzed separately. Results HRT significantly decreased fasting insulin and homeostasis model assessment of insulin resistance in perimenopausal users, and fasting plasma glucose levels in postmenopausal users with prior menstrual disorders, compared with baseline. Furthermore, HRT decreased low-density lipoprotein cholesterol, total cholesterol, fasting insulin, fasting plasma glucose and homeostasis model assessment of insulin resistance in both peri- and postmenopausal controls, compared with baseline. Nevertheless, no significant differences were observed in any of the glucose or lipid metabolism indicators at baseline and follow-up, as well as changes from baseline levels between menopausal women with and without prior menstrual disorders. Conclusions HRT shows more obvious within-group improvements in glucose and lipid metabolism in controls, but there is no significant between-group difference. Further prospective studies are required for confirmation.
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- 2021
10. Conformation-selective tau monoclonal antibodies inhibit tau pathology in primary neurons and a mouse model of Alzheimer’s disease
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Hong Xu, Bin Zhang, Garrett S. Gibbons, Dawn M. Riddle, Mia O’Reilly, Kurt R. Brunden, Emily S. Meymand, Qihui Wu, Virginia M.-Y. Lee, John Q. Trojanowski, Lakshmi Changolkar, Susan Leight, and Soo-Jung Kim
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0301 basic medicine ,Immunoprecipitation ,medicine.drug_class ,media_common.quotation_subject ,tau Proteins ,lcsh:Geriatrics ,Monoclonal antibody ,lcsh:RC346-429 ,Epitope ,law.invention ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Mice ,0302 clinical medicine ,law ,In vivo ,Alzheimer Disease ,mental disorders ,medicine ,Animals ,Humans ,Senile plaques ,Internalization ,Molecular Biology ,lcsh:Neurology. Diseases of the nervous system ,media_common ,Neurons ,Chemistry ,Antibodies, Monoclonal ,Molecular biology ,lcsh:RC952-954.6 ,Disease Models, Animal ,030104 developmental biology ,Tauopathies ,Recombinant DNA ,Immunohistochemistry ,Monoclonal antibodies ,Neurology (clinical) ,Immunotherapy ,Tau ,Alzheimer’s disease ,030217 neurology & neurosurgery ,Research Article - Abstract
BackgroundThe spread of tau pathology in Alzheimer’s disease (AD) is mediated by cell-to-cell transmission of pathological tau seeds released from neurons that, upon internalization by recipient neurons, template the misfolding of naïve cellular tau, thereby propagating fibrillization. We hypothesize that anti-tau monoclonal antibodies (mAbs) that selectively bind to pathological tau seeds will inhibit propagation of tau aggregates and reduce the spread of tau pathology in vivo.MethodsWe inoculated mice with human AD brain-derived extracts containing tau paired helical filaments (AD-tau) and identified two novel mAbs, DMR7 and SKT82, that selectively bind to a misfolded pathological conformation of tau relative to recombinant tau monomer. To evaluate the effects of these mAbs on the spread of pathological tau in vivo,5xFAD mice harboring significant brain Aβ plaque burden were unilaterally injected with AD-tau in the hippocampus, to initiate the formation of neuritic plaque (NP) tau pathology, and were treated weekly with intraperitoneal (i.p.) injections of DMR7, SKT82, or IgG isotype control mAbs.ResultsDMR7 and SKT82 bind epitopes comprised of the proline-rich domain and c-terminal region of tau and binding is reduced upon disruption of the pathological conformation of AD-tau by chemical and thermal denaturation. We found that both DMR7 and SKT82 immunoprecipitate pathological tau and significantly reduce the seeding of cellular tau aggregates induced by AD-tau in primary neurons by 60.5 + 13.8% and 82.2 + 8.3%, respectively, compared to IgG control. To investigate the mechanism of mAb inhibition, we generated pH-sensitive fluorophore-labeled recombinant tau fibrils seeded by AD-tau to track internalization of tau seeds and demonstrate that the conformation-selective tau mAbs inhibit the internalization of tau seeds. DMR7 and SKT82 treatment reduced hyperphosphorylated NP tau as measured with AT8 immunohistochemistry (IHC) staining, but did not achieve statistical significance in the contralateral cortex and SKT82 significantly reduced tau pathology in the ipsilateral hippocampus by 24.2%;p = 0.044.ConclusionsThese findings demonstrate that conformation-selective tau mAbs, DMR7 and SKT82, inhibit tau pathology in primary neurons by preventing the uptake of tau seeds and reduce tau pathology in vivo,providing potential novel therapeutic candidates for the treatment of AD.
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- 2020
11. Current practice and awareness of pediatric off-label drug use in Shanghai, China -a questionnaire-based study
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Mei Mei, Guoying Huang, Xiaobo Zhang, Yonghao Gui, Hong Xu, and Libo Wang
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medicine.medical_specialty ,China ,Off-label drug use ,Attitude of Health Personnel ,Off-label use ,03 medical and health sciences ,0302 clinical medicine ,030225 pediatrics ,Medicine ,Humans ,Shanghai china ,030212 general & internal medicine ,Practice Patterns, Physicians' ,Child ,Children ,Practice ,Practice Patterns, Nurses' ,Practice Patterns, Pharmacists' ,Health professionals ,business.industry ,Questionnaire ,lcsh:RJ1-570 ,lcsh:Pediatrics ,Guideline ,Off-Label Use ,Awareness ,National guideline ,Pediatric drug ,Drug Utilization ,Cross-Sectional Studies ,Guideline implementation ,Current practice ,Family medicine ,Pediatrics, Perinatology and Child Health ,Self Report ,business ,Research Article - Abstract
Background Off-label drug use is widespread in pediatric drug treatment, and the implementation of guidelines on this topic remains challenging. The objective of this study was to evaluate current practice and awareness of healthcare professionals towards pediatric off-label drug use, as well as the barriers to guideline implementation among pediatric healthcare professionals in Shanghai, China. Methods A validated questionnaire was issued to representatives of pediatricians, pharmacists, nurses and administrators from hospitals with pediatric qualification in Shanghai. Results A total of 679 completed questionnaires from 69 hospitals were included in the analysis. Nearly half (47.9%) of the pediatricians acknowledged that they had prescribed off-label drugs. Most (88.4%) of the pharmacists acknowledged that they had dispensed off-label medicines. The main reason for off-label prescribing was the lack of pediatric dosage information. The most common category of off-label prescribing in children was dosage. Nearly half (42.0%) of the participating hospitals had developed internal protocols for off-label drug use. However, approximately half of the respondents reported that they did not adhere to the guidance and that it had barriers to implementation. Most respondents (84.5%) declared that they were familiar with the term “off-label drug use”. However, the awareness rate of the Chinese Expert Consensus of Pediatric Off-Label Drug Use was low (45.7%). More than half (55.4%) of the respondents declared that they did not adhere to the process proposed in the consensus and that barriers existed for its utilization. Conclusions Pediatric off-label drug use is widespread in Shanghai, China, and barriers exist to the implementation of the guideline. A legally recognized national guideline with a broad scope of application for off-label drug use is urgently needed; at the same time, more education and training on off-label drug use should be provided to targeted healthcare professionals. Electronic supplementary material The online version of this article (10.1186/s12887-019-1664-7) contains supplementary material, which is available to authorized users.
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- 2019
12. Expanding the spectrum of A20 haploinsufficiency in two Chinese families: cases report
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Hong Xu, Guomin Li, Wan-Zhen Guan, Li Sun, Haimei Liu, and Bingbing Wu
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0301 basic medicine ,Liver Cirrhosis ,Male ,Arthritis ,Case Report ,Haploinsufficiency ,030105 genetics & heredity ,Child ,Oral Ulcer ,Genetics (clinical) ,Exome sequencing ,Macrophage Activation Syndrome ,Interstitial lung disease ,Rash ,Arthralgia ,Pedigree ,Phenotype ,Child, Preschool ,Female ,medicine.symptom ,medicine.medical_specialty ,lcsh:Internal medicine ,Heterozygote ,lcsh:QH426-470 ,Liver fibrosis ,Folliculitis ,03 medical and health sciences ,Hypothyroidism ,Asian People ,Exome Sequencing ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,A20 haploinsufficiency ,lcsh:RC31-1245 ,Tumor Necrosis Factor alpha-Induced Protein 3 ,Base Sequence ,business.industry ,TNFAIP3 gene ,Perianal Abscess ,medicine.disease ,Inflammatory Bowel Diseases ,Dermatology ,Arthritis, Juvenile ,lcsh:Genetics ,030104 developmental biology ,Macrophage activation syndrome ,Mutation ,business ,Lung Diseases, Interstitial - Abstract
Background The association between mutations in the TNFAIP3 gene and a new autoinflammatory disease (called A20 haploinsufficiency, HA20) has recently been recognized. Here, we describe four patients with HA20 from two unrelated Chinese families. Case presentation A total of four patients from two families were included. The average age at onset was 5.9 years. All patients had no signs of eye or skin problems, such as uveitis, rash, folliculitis and dermal abscess. Prior to the recognition of HA20, P1 was diagnosed with SLE, liver fibrosis and hypothyroidism. She also had no oral, genital or perineal ulcers. P2 was diagnosed with Crohn’s disease and inflammatory bowel disease-related arthritis (IBD-RA). He had a perianal abscess but no oral or genital ulcers. P3, the father of P1 and P2, only had mild oral ulcers, arthralgia, and archosyrinx. P4 was diagnosed with polyarticular juvenile idiopathic arthritis (JIA), macrophage activation syndrome (MAS) and interstitial lung disease (ILD). Whole exome sequencing (WES) was performed in two families. WES revealed heterozygous c.559C > T in the TNFAIP3 gene in P1, P2 and P3, while the c.259C > T mutation in the TNFAIP3 gene was identified in P4. The c.259C > T mutations is novel. Conclusion HA20 had a different phenotype between families and even between family members with the same mutation. Liver fibrosis, hypothyroidism, ILD and MAS in the patients with HA20 were first reported in this study. Our results expanded the phenotype and genotype spectrum of A20 haploinsufficiency. Electronic supplementary material The online version of this article (10.1186/s12881-019-0856-1) contains supplementary material, which is available to authorized users.
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- 2019
13. The LIM protein Ajuba/SP1 complex forms a feed forward loop to induce SP1 target genes and promote pancreatic cancer cell proliferation
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Bosen Zhang, Hao Chen, Mengying Li, Hong Xu, Jiamin Wang, Lei Li, Jiali Cai, Liwei Song, Hao Jia, Zhaoyuan Hou, and Minmin Shi
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0301 basic medicine ,Adult ,Male ,Transcriptional Activation ,Cancer Research ,Chromatin Immunoprecipitation ,Sp1 Transcription Factor ,Biology ,Co-activator ,lcsh:RC254-282 ,FunRich ,03 medical and health sciences ,0302 clinical medicine ,Transcription (biology) ,Cell Line, Tumor ,Gene expression ,Ajuba ,Humans ,Promoter Regions, Genetic ,Transcription factor ,Aged ,Cell Proliferation ,Aged, 80 and over ,Messenger RNA ,Sp1 transcription factor ,Cell growth ,Research ,PDAC ,LIM Domain Proteins ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Cell biology ,SP1 ,Gene Expression Regulation, Neoplastic ,Pancreatic Neoplasms ,cBioPortal ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Multiprotein Complexes ,Female ,Protein Ajuba ,Chromatin immunoprecipitation ,Protein Binding - Abstract
Background The aim of this study is to explore the molecular mechanism of the LIM protein Ajuba and the transcription factor SP1 in the pathogenesis and progression of PDAC. Ajuba is a newly defined transcriptional co-regulator and plays important role in various cancer development, while SP1 is a classic transcription factor, and is closely related with a variety of gene expression and cancer development including PDAC. Methods The expression of Ajuba and SP1 in PDAC tissues was detected by immunohistochemistry (IHC), and the correlation between expression level and clinical prognosis of Ajuba and SP1 was extensively analyzed using online tools. The interaction between Ajuba and SP1 was examined by co-immunoprecipitation (co-IP) and GST-pulldown assays. Stable cell lines were established via lentiviral infection, and was examined by qRT-PCR and western blot assays. The effects of Ajuba/SP1 on PDAC cell proliferation were examined using CCK8 and colony formation assays. Luciferase reporter and chromatin immunoprecipitation (ChIP) assays were employed to examine the transcription activity. Results The expression level (protein and mRNA) of Ajuba and SP1 was elevated in PDAC tissues and was positively correlated; patients with high Ajuba and SP1 expression had a poor prognosis. Mechanistically, Ajuba binds to the C-terminus of SP1 and functions as a co-activator to enhance SP1 gene expression and promote cell proliferation; the promoter of Ajuba contains functional SP1 responsive elements and Ajuba itself is a target gene of SP1. Conclusion Ajuba functions as a co-activator of SP1 to induce its target gene, and that Ajuba itself is a target genes of SP1. Ajuba/SP1 complex could form a feed forward loop to drive SP1 target gene transcription and promote cell proliferation of pancreatic cancer cells. Ajuba and SP1 might be biomarkers for PDAC diagnostics, prognosis and targets for new therapeutics.
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- 2019
14. A new Schiff base coordinated copper(II) compound induces apoptosis and inhibits tumor growth in gastric cancer
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Nan Zhang, Chaoying Li, Luping Zhang, Jinzhu Zhang, Hong Xu, Yan Xia, Xingkai Liu, and Yan Li
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Cancer Research ,Cell cycle checkpoint ,Schiff base copper coordination compound ,lcsh:RC254-282 ,NF-κB ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Genetics ,Autophagy ,lcsh:QH573-671 ,IC50 ,Cisplatin ,Chemistry ,lcsh:Cytology ,Cancer ,ROS ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Oncology ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Primary Research ,medicine.drug - Abstract
Background Gastric cancer, as a multifactorial disorders, shows cytological and architectural heterogeneity compared to other gastrointestinal cancers, making it therapeutically challenging. Cisplatin is generally used in clinic for gastric cancer treatment but with toxic side effects and develops resistance. Anti-tumor properties of copper and its coordinated compounds have been explored intensively in recent years. Methods In this study, we synthesized a novel Schiff base copper coordinated compound (SBCCC) and examined its antitumor effects in two gastric cancer cell lines SGC-7901 and BGC-823 as well as a mouse model of gastric cancer. Results The results show that SBCCC can significantly inhibit the proliferation of gastric cancer cells in a dose- and time-dependent manner. The IC50 of SBCCC in SGC-7901 and BGC-823 cells is 1 μM, which is much less than cisplatin’s IC50. SBCCC induces apoptosis and causes cell cycle arrest at the G1 phase. SBCCC induces apoptosis via multiple pathways including inhibition of NF-κB, ROS production and autophagy. Conclusions The synthesized SBCCC induced cancer cell death via inhibition of NF-κB, ROS production and autophagy. The multiple cell-killing mechanisms were important to overcome therapeutic failure because of multidrug-resistance of cancer cells. SBCCC, with a lower IC50 compared to cisplatin, could render it the potential to overcome the side-effect for clinical application.
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- 2019
15. pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor
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Jialu Liu, Jiaojiao Liu, Haimei Liu, Yihui Zhai, Franz Schaefer, Hong Xu, Qian Shen, Jing Chen, and Claus Peter Schmitt
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0301 basic medicine ,lcsh:QH426-470 ,Bicarbonate ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,610 Medical sciences Medicine ,Downregulation and upregulation ,Gene expression ,Peritoneal dialysis fluids ,Humans ,lcsh:QH573-671 ,Enhancer ,Promoter Regions, Genetic ,Molecular Biology ,Transcription factor ,Gene ,Binding Sites ,Aquaporin 1 ,lcsh:Cytology ,pH ,Promoter ,Transfection ,Hydrogen-Ion Concentration ,Cell biology ,Up-Regulation ,DNA-Binding Proteins ,lcsh:Genetics ,030104 developmental biology ,HEK293 Cells ,chemistry ,Gene Knockdown Techniques ,Mutagenesis, Site-Directed ,Transcription ,Research Article ,Transcription Factors - Abstract
Background Bicarbonate-based peritoneal dialysis (PD) fluids enhance the migratory capacity and damage-repair ability of human peritoneal mesothelial cells by upregulating AQP1. However, little is known about the underlying molecular mechanisms. Results Here we used HEK-293T cells to investigate the effect of pH on AQP1 gene transcription levels. We found that AQP1 mRNA levels increases with pH. Transfection of HEK-293T cells with luciferase reporter vectors containing different regions of the AQP1 promoter identified an upstream region in the AQP1 gene between − 2200 and – 2300 bp as an enhancer required for pH-mediated regulation of AQP1 expression. Site-directed mutagenesis of this specific promoter region revealed a critical region between − 2257 and − 2251 bp, and gene knock-down experiments and ChIP assays suggested that the Spi-B transcription factor SPIB is involved in pH-mediated regulation of AQP1 expression. Conclusions We identified an upstream region in the AQP1 gene and the transcription factor SPIB that are critically involved in pH-mediated regulation of AQP1 expression. These findings provide the basis for further studies on the pH- and buffer-dependent effects of PD fluids on peritoneal membrane integrity and function. Electronic supplementary material The online version of this article (10.1186/s12867-018-0104-9) contains supplementary material, which is available to authorized users.
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- 2018
16. Multiresistant ST59-SCCmec IV-t437 clone with strong biofilm-forming capacity was identified predominantly in MRSA isolated from Chinese children
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Yingchao Liu, Hongyan Zheng, Jinghui Zhen, Lijuan Wang, Fang Dong, Hong Xu, Wenqi Song, Kaihu Yao, Wenting Li, Xin Yang, Suyun Qian, and Wei Zhou
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0301 basic medicine ,Male ,Methicillin-Resistant Staphylococcus aureus ,Staphylococcus aureus ,China ,Adolescent ,030106 microbiology ,Mupirocin ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Antimicrobial resistance ,Microbiology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,chemistry.chemical_compound ,Clonal lineage ,Pediatrician ,Drug Resistance, Multiple, Bacterial ,Trimethoprim, Sulfamethoxazole Drug Combination ,medicine ,Humans ,lcsh:RC109-216 ,Child ,SCCmec ,Biofilm ,Infant, Newborn ,Linezolid ,Infant ,biochemical phenomena, metabolism, and nutrition ,Staphylococcal Infections ,bacterial infections and mycoses ,Trimethoprim ,Penicillin ,Infectious Diseases ,chemistry ,Biofilms ,Child, Preschool ,Vancomycin ,Multilocus sequence typing ,Female ,medicine.drug ,Research Article ,Multilocus Sequence Typing - Abstract
Background This study aimed to investigate the clinical and molecular epidemiology and biofilm formation of Staphylococcus aureus (SA) isolated from pediatricians in China. Methods SA strains were isolated from Beijing Children’s hospital from February 2016 to January 2017. Isolates were typed by multilocus sequence typing (MLST), spa and SCCmec typing (for Methicillin-resistant SA [MRSA] only). Antimicrobial susceptibility testing was performed by agar dilution method except sulphamethoxazole/trimethoprim (E-test method). Biofilm formation and biofilm associated genes were detected. Results Totally 104 children (41 females and 63 males; median age, 5.2 months) were enrolled in this study, in which 60 patients suffered from MRSA infection. Among the 104 cases, 54.8% were categorized as community associated SA (CA-SA) infections. The children under 3 years were more likely to occur CA-SA infections compared with older ones (P = 0.0131). ST59-SCCmec IV-t437 (61.7%) was the most prevalent genotype of MRSA, and ST22-t309 (18.2%), ST5-t002 (9.1%), ST6-t701 (9.1%), ST188-t189 (9.1%) were the top four genotypes of methicillin-sensitive SA (MSSA). All the present isolates were susceptible to linezolid, vancomycin, trimethoprim-sulfamethoxazole, mupirocin, tigecyclin, fusidic acid. No erythromycin-susceptible isolate was determined, and only a few isolates (3.8%) were identified as susceptible to penicillin. Multi-drug resistant isolates were reponsible for 83.8% of the ST59-SCCmec IV-t437 isolates. The isolates with strong biofilm formation were found in 85% of MRSA and 53.2% of MSSA, and in 88.7% of ST59-SCCmec IV-t437 isolates. Biofilm formation ability varied not only between MRSA and MSSA (P = 0.0053), but also greatly among different genotypes (P
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- 2017
17. Expert consensus on the prevention, diagnosis and treatment of cold injury in China, 2020.
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Jin, Hong-Xu, Teng, Yue, Dai, Jing, Zhao, Xiao-Dong, Members of the Emergency Medicine Committee of the People's Liberation Army, Cao, Yang, Chen, Cong, Chen, Wei, Fan, Chen-fang, Fei, Jun, Gan, Le-Wen, Guo, Ying-Fei, He, Jian, Huang, Zhi-Gang, Ji, Bin-Long, Li, Nan, Li, Wei-Qin, Liu, Hong-Sheng, Liu, Ming-Hua, and Liu, Shuang-Qing
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EMERGENCY medicine ,PREVENTION of injury ,EPIDEMIOLOGY ,DATA analysis - Abstract
Cold injury refers to local or systemic injury caused by a rapid, massive loss of body heat in a cold environment. The incidence of cold injury is high. However, the current situation regarding the diagnosis and treatment of cold injury in our country is not ideal. To standardize and improve the level of clinical diagnosis and treatment of cold injury in China, it is necessary to make a consensus that is practical and adapted to the conditions in China. We used the latest population-level epidemiological and clinical research data, combined with relevant literature from China and foreign countries. The consensus was developed by a joint committee of multidisciplinary experts. This expert consensus addresses the epidemiology, diagnosis, on-site emergency procedures, in-hospital treatment, and prevention of cold injury. [ABSTRACT FROM AUTHOR]
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- 2021
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18. Electrolyte disorders and aging: risk factors for delirium in patients undergoing orthopedic surgeries
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Lihong Wang, Guo Hong Xu, Xian Jiao Wei, Dong Juan Xu, and Hao Teng Chang
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Male ,medicine.medical_specialty ,Aging ,China ,Age-related risk factor ,Comorbidity ,Logistic regression ,03 medical and health sciences ,Electrolytes ,0302 clinical medicine ,Postoperative Complications ,Risk Factors ,mental disorders ,Medicine ,Humans ,Orthopedic Procedures ,030212 general & internal medicine ,Aged ,Retrospective Studies ,Orthopedic surgery ,Aged, 80 and over ,Hypocalcemia ,business.industry ,Incidence (epidemiology) ,Incidence ,Postoperative delirium ,Delirium ,Retrospective cohort study ,Odds ratio ,Middle Aged ,medicine.disease ,Surgery ,Psychiatry and Mental health ,Anesthesia ,Electrolyte disorders ,Potassium ,Female ,medicine.symptom ,Chlorine ,Calcium disorder ,business ,Hyponatremia ,030217 neurology & neurosurgery ,Research Article - Abstract
Background At present, the exact mechanism of postoperative delirium has not been elucidated. The purpose of this study was to analyze the incidence of delirium in patients undergoing orthopedic surgeries and to explore possible related factors. Methods This is a retrospective study. We used 582 patients who had undergone orthopedic surgery between January 2011 and December 2014. The surgeries consisted of 155 cases of internal fixation for intertrochanteric fracture (IFIF), 128 cases of femoral head replacement (FHR), 169 cases of total hip arthroplasty (THA) and 130 cases of total knee arthroplasty (TKA). Among the 582 patients, 75 developed postoperative delirium (an incidence of 12.9%). The demographics of the patients, which included age, gender, operation duration and blood loss, were statistically analyzed with univariate logistic regression analysis and then multivariate logistic regression. To investigate the influences of different electrolytes disorders for postoperative delirium, the Chi-square test was used. Results Multivariate logistic regression analysis indicated that postoperative delirium incidence in patients aged 70–79 years and in patients aged ≥80 years was higher than that in patients aged
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- 2016
19. LncRNA NONRATT021972 siRNA regulates neuropathic pain behaviors in type 2 diabetic rats through the P2X7 receptor in dorsal root ganglia
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Shouyu Wang, Lifang Zou, Jinyan Xie, Chunping Zhang, Yun Gao, Changshui Xu, Shuangmei Liu, Xi Zhang, Hong Xu, Guilin Li, Shangdong Liang, Wei Xie, Shiyao Wen, Yun Xue, Qiulan Lv, Qiuyu Xie, and Bing Wu
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0301 basic medicine ,Nervous system ,Neural Conduction ,Fluorescent Antibody Technique ,0302 clinical medicine ,Adenosine Triphosphate ,Ganglia, Spinal ,RNA, Small Interfering ,Dorsal root ganglia ,Glial fibrillary acidic protein ,biology ,Behavior, Animal ,Transfection ,medicine.anatomical_structure ,Hyperalgesia ,Neuropathic pain ,P2X7 receptor ,Tumor necrosis factor alpha ,RNA, Long Noncoding ,medicine.symptom ,Ion Channel Gating ,Protein Binding ,medicine.medical_specialty ,Sensory Receptor Cells ,Diabetes Mellitus, Experimental ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Internal medicine ,Glial Fibrillary Acidic Protein ,medicine ,Animals ,Humans ,RNA, Messenger ,Molecular Biology ,Messenger RNA ,business.industry ,Tumor Necrosis Factor-alpha ,Research ,RNA ,030104 developmental biology ,Endocrinology ,HEK293 Cells ,Diabetes Mellitus, Type 2 ,Gene Expression Regulation ,biology.protein ,Diabetic neuropathic pain ,Neuralgia ,Receptors, Purinergic P2X7 ,business ,Neuroscience ,030217 neurology & neurosurgery ,Long noncoding RNA - Abstract
Background Long non-protein-coding RNAs (lncRNAs) are involved in the pathological processes of nervous system diseases. NONRATT021972 is an lncRNA. This study explores the effects of lncRNA NONRATT021972 small interference RNA (siRNA) on diabetic neuropathic pain (DNP) mediated by the P2X7 receptor in the rat dorsal root ganglia (DRG). Results Our results show that NONRATT021972 expression was significantly higher in the DRG of diabetes mellitus (DM) group compared with control group. NONRATT021972 expression in the DRG was reduced when DM rats were treated with NONRATT021972 siRNA. NONRATT021972 siRNA treatment in type 2 DM rats increased the mechanical withdrawal threshold (MWT), the thermal withdrawal latency (TWL) and the sensory nerve conduction velocity (SNCV) of rat tail nerves. After intravenous injection with NONRATT021972 siRNA in DM rats, the P2X7, GFAP and TNF-ɑ expression levels in DRG were decreased. An interaction between the RNA (NONRATT021972) and protein (P2X7) was predicted by the application of bioinformatics technology. The BzATP-activated currents in DRG non-neurons (satellite glial cells) of DM rats were significantly increased compared to control rats. NONRATT021972 siRNA treatment inhibited the ATP-activated currents in HEK293 cells transfected with pEGFP-P2X7. Conclusions NONRATT021972 siRNA treatment can decrease the expression levels of P2X7 mRNA and protein and inhibit the activation of satellite glial cells (SGCs) in the DRG of type 2 DM rats. Moreover, NONRATT021972 siRNA treatment reduced the release of inflammatory factors (TNF-α), thereby inhibiting the excitability of DRG neurons and reducing mechanical and thermal hyperalgesia in type 2 DM rats.
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- 2016
20. Incidence of breast cancer in Chinese women exposed to the 1959-1961 great Chinese famine.
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Dandan He, Yuan Fang, Gunter, Marc J., Dongli Xu, Yanping Zhao, Jie Zhou, Hong Fang, Wang Hong Xu, He, Dandan, Fang, Yuan, Xu, Dongli, Zhao, Yanping, Zhou, Jie, Fang, Hong, and Xu, Wang Hong
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BREAST cancer patients ,DISEASE incidence ,MALNUTRITION ,CHINESE people ,MEDICAL education ,MEDICAL records ,DISEASES - Abstract
Background: The association of malnutrition in early life with breast cancer risk has been studied in Europe by investigating survivors of the Dutch Hunger Winter Famine, but not in China. We evaluated the effect of exposure to the 1959-1961 Great Leap Forward famine on subsequent breast cancer risk in Chinese women.Methods: A total of 59,060 women born in 1955~1966 were recruited from Minhang district, Shanghai, China, during the period 2008 to 2012. A baseline survey was conducted to collect demographic characteristics and known risk factors for breast cancer. Incident breast cancers were identified by conducting record linkage with the Shanghai Cancer Registry up to June 30, 2015, and confirmed through medical records. Cumulative probabilities of cancer incidence were evaluated after adjusting for age, educational level and other confounders. Cox regression models were applied to estimate the hazard ratios (HR) and 95% confidence intervals (CI) of breast cancer.Results: The overall crude incidence of in situ and invasive breast cancer were 19.2 and 115.0 per 100,000, respectively, in women conceived or born during the famine (1959-1962), slightly higher than those in women born before (1955-1958) (13.2 and 109.8/100,000) and after (1963-1966) (10.4 and 101.5/100,000). Particularly, at age group of 50-52 years when all participants contributed person-year of observations, the age-specific incidence of invasive breast cancer was higher in pre-natal exposed women (123.7/100,000, 95%CI: 94.5-161.9/100,000) than in post-natal exposed (109.6/100,000, 95%CI: 69.1-174.0/100,000) and unexposed women (82.7/100,000, 95%CI: 46.9-145.7/100,000). However, the incidence of cancer in situ was slightly lower in pre-natal exposed women at the age group. Adjusted cumulative probabilities of breast cancer incidence, both in-situ and invasive, were also observed to be higher in women exposed to the famine, however, the difference was not statistically significant.Conclusion: Our results suggest a possible adverse, but limited, impact of exposure to the Great famine on the risk of breast cancer in Chinese women. [ABSTRACT FROM AUTHOR]- Published
- 2017
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21. Multiresistant ST59-SCCmec IV-t437 clone with strong biofilm-forming capacity was identified predominantly in MRSA isolated from Chinese children.
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Xin Yang, Suyun Qian, Kaihu Yao, Lijuan Wang, Yingchao Liu, Fang Dong, Wenqi Song, Jinghui Zhen, Wei Zhou, Hong Xu, Hongyan Zheng, Wenting Li, Yang, Xin, Qian, Suyun, Yao, Kaihu, Wang, Lijuan, Liu, Yingchao, Dong, Fang, Song, Wenqi, and Zhen, Jinghui
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BIOFILMS ,MOLECULAR epidemiology ,MICROBIAL virulence ,MICROBIAL sensitivity tests ,STAPHYLOCOCCUS aureus ,DRUG resistance in microorganisms ,MUPIROCIN ,RESEARCH funding ,STAPHYLOCOCCAL diseases ,METHICILLIN-resistant staphylococcus aureus ,SEQUENCE analysis ,PHARMACODYNAMICS ,PHYSIOLOGY - Abstract
Background: This study aimed to investigate the clinical and molecular epidemiology and biofilm formation of Staphylococcus aureus (SA) isolated from pediatricians in China.Methods: SA strains were isolated from Beijing Children's hospital from February 2016 to January 2017. Isolates were typed by multilocus sequence typing (MLST), spa and SCCmec typing (for Methicillin-resistant SA [MRSA] only). Antimicrobial susceptibility testing was performed by agar dilution method except sulphamethoxazole/trimethoprim (E-test method). Biofilm formation and biofilm associated genes were detected.Results: Totally 104 children (41 females and 63 males; median age, 5.2 months) were enrolled in this study, in which 60 patients suffered from MRSA infection. Among the 104 cases, 54.8% were categorized as community associated SA (CA-SA) infections. The children under 3 years were more likely to occur CA-SA infections compared with older ones (P = 0.0131). ST59-SCCmec IV-t437 (61.7%) was the most prevalent genotype of MRSA, and ST22-t309 (18.2%), ST5-t002 (9.1%), ST6-t701 (9.1%), ST188-t189 (9.1%) were the top four genotypes of methicillin-sensitive SA (MSSA). All the present isolates were susceptible to linezolid, vancomycin, trimethoprim-sulfamethoxazole, mupirocin, tigecyclin, fusidic acid. No erythromycin-susceptible isolate was determined, and only a few isolates (3.8%) were identified as susceptible to penicillin. Multi-drug resistant isolates were reponsible for 83.8% of the ST59-SCCmec IV-t437 isolates. The isolates with strong biofilm formation were found in 85% of MRSA and 53.2% of MSSA, and in 88.7% of ST59-SCCmec IV-t437 isolates. Biofilm formation ability varied not only between MRSA and MSSA (P = 0.0053), but also greatly among different genotypes (P < 0.0001). The prevalence of the biofilm associated genes among ST59-SCCmec IV-t437 clone was: icaA (100.0%), icaD (97.3%), fnbpA (100.0%), fnbpB (0), clfA (100%), clfB (100%), cna (2.7%), bbp (0), ebpS (88.5%), sdrC (78.4%), sdrD (5.4%), and sdrE (94.5%).Conclusions: These results indicated strong homology of the MRSA stains isolated from Chinese children, which was caused by spread of multiresistant ST59-SCCmec IV-t437 clone with strong biofilm formation ability. The MSSA strains, in contrast, were very heterogeneity, half of which could produce biofilm strongly. [ABSTRACT FROM AUTHOR]- Published
- 2017
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22. Molecular characteristic of mcr-1 producing Escherichia coli in a Chinese university hospital.
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Qing-wen He, Xiao-hong Xu, Fang-jun Lan, Zhi-chang Zhao, Zhi-yun Wu, Ying-ping Cao, and Bin Li
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ESCHERICHIA coli ,PATHOGENIC microorganisms ,METHICILLIN-resistant staphylococcus aureus ,DRUG efficacy ,UNIVERSITY hospitals - Abstract
Background: Colistin has been considered as a last-line treatment option in severe infections caused by multidrug-resistant (MDR) gram-negative pathogens. However, the emergence of the mobile colistin resistance gene (mcr-1) has challenged this viewpoint. The aim of this study is to explore the prevalence of mcr-1 in Escherichia coli (E. coli) in a Chinese teaching hospital, and investigate their molecular characteristics. Methods: A total of 700 E. coli isolates were used to screen mcr-1 by PCR and sequencing in a Chinese university hospital from August 2014 to August 2015. Susceptibility test of mcr-1-producing isolates was determined by Vitek -2 Compact system. 26 virulence factors (VFs), phylogenetic groups, Multi-locus sequence typing (MLST), and DNA Fingerprinting (ERIC-PCR) of strains were investigated by PCR. Results: Four (0.6%) mcr-1 producing E. coli isolates were found in this study. The results of antibiotic susceptibility test showed that all four isolates were resistant to colistin, ciprofloxacin, levofloxacin, cefazolin, and trimethoprim/sulfamethoxazole, and were susceptible to amikacin, ertapenem and imipenem. In addition, all 4 isolates exhibited high-level resistance to aztreonam, cefotaxime and gentamicin. The numbers of VFs contained in mcr-1 positive isolates were no more than 4 in our study. MLST result demonstrated that these isolates were assigned to two sequence types: ST156 and ST167. The result of phylogenetic analysis showed that four mcr-1-positive isolates belong to two phylogenetic groups: A and B1 group. ERIC-PCR showed that four mcr-1 positive strains were categorized into three different genotypes. Conclusions: Our study demonstrated a low prevalence of mcr-1 in E. coli clinical isolates in a Chinese teaching hospital, and we have gained insights into the molecular characteristics of these mcr-1-positive strains. Increasing the surveillance of these infections, as well as taking effective infection control measures are urgently needed to take to control the transmission of mcr-1 gene. [ABSTRACT FROM AUTHOR]
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- 2017
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23. Anterior inferior plating versus superior plating for clavicle fracture: a meta-analysis.
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Jie Ai, Shun-Li Kan, Hai-Liang Li, Hong Xu, Yang Liu, Guang-Zhi Ning, Shi-Qing Feng, Ai, Jie, Kan, Shun-Li, Li, Hai-Liang, Xu, Hong, Liu, Yang, Ning, Guang-Zhi, and Feng, Shi-Qing
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CLAVICLE injury treatment ,FRACTURE fixation ,BLOOD loss estimation ,SURGICAL complications ,META-analysis ,RANDOMIZED controlled trials - Abstract
Background: The position of plate fixation for clavicle fracture remains controversial. Our objective was to perform a comprehensive review of the literature and quantify the surgical parameters and clinical indexes between the anterior inferior plating and superior plating for clavicle fracture.Methods: PubMed, EMBASE, and the Cochrane Library were searched for randomized and non-randomized studies that compared the anterior inferior plating with the superior plating for clavicle fracture. The relative risk or standardized mean difference with 95% confidence interval was calculated using either a fixed- or random-effects model.Results: Four randomized controlled trials and eight observational studies were identified to compare the surgical parameters and clinical indexes. For the surgical parameters, the anterior inferior plating group was better than the superior plating group in operation time and blood loss (P < 0.05). Furthermore, in terms of clinical indexes, the anterior inferior plating was superior to the superior plating in reducing the union time, and the two kinds of plate fixation methods were comparable in constant score, and the rate of infection, nonunion, and complications (P > 0.05).Conclusions: Based on the current evidence, the anterior inferior plating may reduce the blood loss, the operation and union time, but no differences were observed in constant score, and the rate of infection, nonunion, and complications between the two groups. Given that some of the studies have low quality, more randomized controlled trails with high quality should be conduct to further verify the findings. [ABSTRACT FROM AUTHOR]- Published
- 2017
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24. Allograft tendon reconstruction of the anterior talofibular ligament and calcaneofibular Ligament in the treatment of chronic ankle instability.
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Weikai Wang, Guo Hong Xu, Wang, Weikai, and Xu, Guo Hong
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ANKLE fractures , *ANKLE lateral ligament , *DORSIFLEXION , *PERONEAL tendons , *JOINTS (Anatomy) , *ANKLE surgery , *LIGAMENT surgery , *TENDON transplantation , *ARTHROPLASTY , *CHRONIC diseases , *JOINT hypermobility , *RETROSPECTIVE studies - Abstract
Background: The purpose was retrospectively to investigate functional and clinical outcomes after anterior talofibular ligament (ATFL) and calcaneofibular ligament (CFL) reconstruction using a single allograft.Methods: Patients with severe chronic lateral instability of the ankle underwent surgery after conservative treatment failed. Ultrasounds of the ankle were performed, and if the AFTL and CFL were completely torn without enough soft tissue for repair, the ligaments were reconstructed using allograft tendon. Outcomes were assessed by clinical examination, stress radiography, ultrasound, the American Orthopaedic Foot and Ankle Society score (AOFAS), and Karlsson Ankle Functional score (KAFS) before surgery and at final follow-up.Results: Nineteen patients, ten men and nine women with mean age of 27.9 years (range, 19-41 years), underwent reconstruction. Mean follow-up was 30 months (range, 24-40 months). At final follow-up, all patients had returned to activity without instability, pain, or limited range of motion. On stress radiography, mean talar tilt angle decreased from 17.32° ± 3.58° before surgery to 4.16° ± 1.12° at follow-up (p < 0.001). Mean anterior drawer test (ADT) distance decreased from 9.79 ± 1.01 mm before surgery to 3.97 ± 0.99 mm at follow-up (p < 0.05). Mean AOFAS improved from 64.00 ± 18.43 to 90.32 ± 5.17 points (p < 0.001), and mean KAFS improved from 50.84 ± 16.73 to 90.89 ± 5.08 points (p < 0.001). Ultrasound showed the reconstructed ligaments maintained good continuity and excellent tension. No case of infection and immunological rejection was reported.Conclusion: This novel reconstruction technique takes into account the anatomical specialty of AFTL and CFL. This case series showed increased stability of the ankle in clinical and functional outcomes.Trial Registration: The trial registration number (TRN) and date of registration: ChiCTR-ORC-17010796 , Mar 6th 2017. Retrospectively registered. [ABSTRACT FROM AUTHOR]- Published
- 2017
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25. Dibutyryl-cAMP attenuates pulmonary fibrosis by blocking myofibroblast differentiation via PKA/CREB/CBP signaling in rats with silicosis.
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Yan Liu, Hong Xu, Yucong Geng, Dingjie Xu, Lijuan Zhang, Yi Yang, Zhongqiu Wei, Bonan Zhang, Shifeng Li, Xuemin Gao, Ruimin Wang, Xianghong Zhang, Darrell Brann, Fang Yang, Liu, Yan, Xu, Hong, Geng, Yucong, Xu, Dingjie, Zhang, Lijuan, and Yang, Yi
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MYOFIBROBLASTS , *SILICOSIS , *ENZYME-linked immunosorbent assay , *CYCLIC-AMP-dependent protein kinase , *WESTERN immunoblotting , *DIAGNOSIS - Abstract
Background: Myofibroblasts play a major role in the synthesis of extracellular matrix (ECM) and the stimulation of these cells is thought to play an important role in the development of silicosis. The present study was undertaken to investigate the anti-fibrotic effects of dibutyryl-cAMP (db-cAMP) on rats induced by silica.Methods: A HOPE MED 8050 exposure control apparatus was used to create the silicosis model. Rats were randomly divided into 4 groups: 1)controls for 16 w; 2)silicosis for 16 w; 3)db-cAMP pre-treatment; 4) db-cAMP post-treatment. Rat pulmonary fibroblasts were cultured in vitro and divided into 4 groups as follows: 1) controls; 2) 10-7mol/L angiotensin II (Ang II); 3) Ang II +10-4 mol/L db-cAMP; and 4) Ang II + db-cAMP+ 10-6 mol/L H89. Hematoxylin-eosin (HE), Van Gieson staining and immunohistochemistry (IHC) were performed to observe the histomorphology of lung tissue. The levels of cAMP were detected by enzyme immunoassay. Double-labeling for α-SMA with Gαi3, protein kinase A (PKA), phosphorylated cAMP-response element-binding protein (p-CREB), and p-Smad2/3 was identified by immunofluorescence staining. Protein levels were detected by Western blot analysis. The interaction between CREB-binding protein (CBP) and Smad2/3 and p-CREB were measured by co-immunoprecipitation (Co-IP).Results: Db-cAMP treatment reduced the number and size of silicosis nodules, inhibited myofibroblast differentiation, and extracellular matrix deposition in vitro and in vivo. In addition, db-cAMP regulated Gαs protein and inhibited expression of Gαi protein, which increased endogenous cAMP. Db-cAMP increased phosphorylated cAMP-response element-binding protein (p-CREB) via protein kinase A (PKA) signaling, and decreased nuclear p-Smad2/3 binding with CREB binding protein (CBP), which reduced activation of p-Smads in fibroblasts induced by Ang II.Conclusions: This study showed an anti-silicotic effect of db-cAMP that was mediated via PKA/p-CREB/CBP signaling. Furthermore, the findings offer novel insight into the potential use of cAMP signaling for therapeutic strategies to treat silicosis. [ABSTRACT FROM AUTHOR]- Published
- 2017
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26. Hypoxia-inducible microRNA-224 promotes the cell growth, migration and invasion by directly targeting RASSF8 in gastric cancer.
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Chuan He, Libo Wang, Jiantao Zhang, and Hong Xu
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MICRORNA ,DEVELOPMENTAL biology ,GROWTH factors ,CARCINOGENS ,HYPOXIA (Water) - Abstract
Background: Hypoxia plays an important role in the development of various cancers. MicroRNAs (miRNAs) act as post-transcriptional regulators of gene expression and modulate the tumorigenesis, including gastric cancer. However, the roles and molecular mechanism of miR-224 in gastric cancer under hypoxia remain poorly understood. Method: Real-time PCR and Northern blot assay were used to examine the effects of hypoxia and HIF-1α on miR-224 expression. Luciferase and ChIP assays were performed to determine whether miR-224 was a transcriptional target of HIF-1α. Then MTT, colony formation, in vitro scratch and invasion assays were used to detect the effects of miR-224 on cell growth, migration and invasion under hypoxia, as well as the in vivo animal study. Luciferase assay andWestern blot were performed to validate the targets of miR-224. Functional studies were performed to determine the roles of RASSF8 as that of miR-224 under hypoxia. The effects of RASSF8 knockdown on the transcriptional activity and translocation of NF-κB were investigated using Luciferase assay and Western blot, respectively. Finally, the expression levels of miR-224 and RASSF8 were detected using real-time PCR in gastric cancer tissues as well as lymph node metastasis tissues. Results: We demonstrated that miR-224 was upregulated by hypoxia and HIF-1α. HIF-1α affected miR-224 expression at the transcriptional level. MiR-224 inhibition suppressed cell growth, migration and invasion induced by hypoxia, while miR-224 overexpression resulted in opposite effects. MiR-224 inhibition also suppressed tumor growth in vivo. We then validated that RASSF8 was a direct target of miR-224. RASSF8 overexpression inhibited cell growth and invasion, while RASSF8 knockdown ameliorated the inhibitory effects of miR-224 inhibition on cell growth and invasion. Furthermore, we found that RASSF8 knockdown enhanced the transcriptional activity of NF-κB and p65 translocation, while RASSF8 overexpression resulted in opposite effects. Inhibition of NF-κB activity by PDTC attenuated the effects of RASSF8 knockdown on cell proliferation and invasion. Finally, miR-224 was upregulated in both gastric cancer tissues and lymph node metastasis positive tissues, while RASSF8 expression was opposite to that of miR-224. Conclusion: These results indicate that hypoxia-inducible miR-224 promotes gastric cancer cell growth, migration and invasion by downregulating RASSF8 and acts as an oncogene, implying that inhibition of miR-224 may have potential as a therapeutic target for patients with hypoxic gastric tumors. [ABSTRACT FROM AUTHOR]
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- 2017
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27. Electrolyte disorders and aging: risk factors for delirium in patients undergoing orthopedic surgeries.
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Li-Hong Wang, Dong-Juan Xu, Xian-Jiao Wei, Hao-Teng Chang, and Guo-Hong Xu
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RISK of delirium ,DELIRIUM ,ORTHOPEDIC surgery ,WATER-electrolyte imbalances ,POSTOPERATIVE period ,PATIENTS ,THERAPEUTICS - Abstract
Background: At present, the exact mechanism of postoperative delirium has not been elucidated. The purpose of this study was to analyze the incidence of delirium in patients undergoing orthopedic surgeries and to explore possible related factors. Methods: This is a retrospective study. We used 582 patients who had undergone orthopedic surgery between January 2011 and December 2014. The surgeries consisted of 155 cases of internal fixation for intertrochanteric fracture (IFIF), 128 cases of femoral head replacement (FHR), 169 cases of total hip arthroplasty (THA) and 130 cases of total knee arthroplasty (TKA). Among the 582 patients, 75 developed postoperative delirium (an incidence of 12.9%). The demographics of the patients, which included age, gender, operation duration and blood loss, were statistically analyzed with univariate logistic regression analysis and then multivariate logistic regression. To investigate the influences of different electrolytes disorders for postoperative delirium, the Chi-square test was used. Results: Multivariate logistic regression analysis indicated that postoperative delirium incidence in patients aged 70-79 years and in patients aged =80 years was higher than that in patients aged <70 years, odds ratio (OR) values were 6.33 and 26.37, respectively. In addition, the incidence of postoperative delirium in the group of patients with electrolyte disorders was higher than that in the normal group (OR, 2.38). There were statistically significant differences between the delirium group and the non-delirium group in the incidences of the sodium and calcium disorders. Conclusions: Aging and postoperative electrolyte disorders (hyponatremia and hypocalcemia) are risk factors for postoperative delirium in patients undergoing orthopedic surgeries. [ABSTRACT FROM AUTHOR]
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- 2016
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28. Features of complementary and alternative medicine use by patients with coronary artery disease in Beijing: a cross-sectional study
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Jie Wang, Xiao-xue Yan, Ze Zhang, Fuyong Chu, Xingjiang Xiong, and Hong-xu Liu
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Complementary Therapies ,Male ,medicine.medical_specialty ,China ,animal structures ,Cross-sectional study ,Cross sectional study ,Alternative medicine ,Traditional Chinese medicine ,Coronary Artery Disease ,Logistic regression ,Odds ,Coronary artery disease ,Beijing ,Internal medicine ,Surveys and Questionnaires ,Prevalence ,Medicine ,Humans ,In patient ,CAD ,Aged ,Traditional medicine ,business.industry ,CAM use ,General Medicine ,Middle Aged ,medicine.disease ,Cross-Sectional Studies ,Logistic Models ,Complementary and alternative medicine ,Patent herbal medicine ,Female ,business ,Research Article - Abstract
Background Complementary and alternative medicine (CAM) is commonly used in China for the management of coronary artery disease (CAD). However, few studies have been conducted to investigate the prevalence, perceived effectiveness, types, and reasons of CAM use in patients diagnosed with CAD. Methods A cross-sectional study design was adopted. Questionnaires were distributed at the outpatient cardiac clinics of four tertiary-level teaching general hospitals in Beijing. Quantitative data were analyzed using Student’s t-test. Categorical data were analyzed using chi-square test. Logistic regression was employed to explore factors associated with the use of CAM as well as CAM use features in Chinese medicine (CM) hospitals when significant differences were found upon comparisons. Results From May to July, 2009, a total of 600 questionnaires were distributed, and 546 patients with a diagnosis of CAD responded with valid values and were included in the present study. CAM was used by 69.1% of the patients with CAD; the majority (75.9%) of these CAM users believes that CAM is effective. “Few side effects” (49.6%) was the main reason of CAM use; whereas “doubt of effect” (61.5%) was the main reason for non-use. Patent herbal medicine (90.7%) was the most commonly used CAM type. Compared with non-CAM users, CAM users tended to be older (p
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- 2013
29. New congenital anomalies of the kidney and urinary tract and outcomes in Robo2 mutant mice with the inserted piggyBac transposon.
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Jialu Liu, Li Sun, Qian Shen, Xiaohui Wu, Hong Xu, Liu, Jialu, Sun, Li, Shen, Qian, Wu, Xiaohui, and Xu, Hong
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CONGENITAL disorders ,KIDNEY diseases ,URINARY tract infections ,GENE expression ,VESICO-ureteral reflux ,DISEASE incidence ,LABORATORY mice - Abstract
Background: Disruption of ROBO2 in humans causes vesicoureteral reflux (VUR)/congenital anomalies of the kidney and urinary tract (CAKUT). PiggyBac (PB) is a DNA transposon, and its insertion often reduces-but does not eliminate-gene expression. The Robo2 insertion mutant exhibited non-dilating VUR, ureteropelvic junction obstruction (UPJO) not found in reported models. We studied the incidence and outcomes of VUR/CAKUT in this mutant and explored the relationship between Robo2 gene expression and the occurrence and severity of VUR/CAKUT.Methods: The urinary systems of newborn mutants were evaluated via Vevo 770 micro-ultrasound. Some of the normal animals-and all of the abnormal animals-were followed to adulthood and tested for VUR. Urinary obstruction experiments were performed on mice with hydronephrosis. The histology of the kidney and ureter was examined by light microscopy and transmission electron microscopy. Robo2 (PB/PB) mice were crossed with Hoxb7/myr-Venus mice to visualize the location of the ureters relative to the bladder.Results: In Robo2 (PB/PB) mice, PB insertion led to an approximately 50 % decrease in Robo2 gene expression. The most common (27.07 %, 62/229) abnormality was non-dilating VUR, and no statistically significant differences were found between age groups. Approximately 6.97 % displayed ultrasound-detectable CAKUT, and these mice survived to adulthood without improvement. No severe CAKUT were found in Robo2 (PB/+) mice. The refluxing ureters showed disorganized smooth muscle fibers, reduced muscle cell populations, intercellular edema and intracytoplasmic vacuoles in smooth muscle cells. Both UPJ and UVJ muscle defects were noted in Robo2 (PB/PB) mice.Conclusions: Robo2 (PB/PB) mice is the first Robo2-deficient mouse model to survive to adulthood while displaying non-dilating VUR, UPJO, and multiple ureters with blind endings. The genetic background of these mutants may influence the penetrance and severity of the CAKUT phenotypes. VUR and other CAKUT found in this mutant had little chance of spontaneous resolution, and this requires careful follow-up. We reported for the first time that the non-dilated refluxing ureters showed disorganized smooth muscle fibers and altered smooth muscle cell structure, more accurately mimicking the characteristics of human cases. Future studies are required to test the role of Robo2 in the ureteric smooth muscle. [ABSTRACT FROM AUTHOR]- Published
- 2016
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30. LncRNA NONRATT021972 siRNA regulates neuropathic pain behaviors in type 2 diabetic rats through the P2X7 receptor in dorsal root ganglia.
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Shuangmei Liu, Lifang Zou, Jinyan Xie, Wei Xie, Shiyao Wen, Qiuyu Xie, Yun Gao, Guilin Li, Chunping Zhang, Changshui Xu, Hong Xu, Bing Wu, Qiulan Lv, Xi Zhang, Shouyu Wang, Yun Xue, and Shangdong Liang
- Subjects
TYPE 2 diabetes ,NEUROPATHY ,SMALL interfering RNA ,DORSAL root ganglia ,PURINERGIC receptors - Abstract
Background: Long non-protein-coding RNAs (lncRNAs) are involved in the pathological processes of nervous system diseases. NONRATT021972 is an lncRNA. This study explores the effects of lncRNA NONRATT021972 small interference RNA (siRNA) on diabetic neuropathic pain (DNP) mediated by the P2X7 receptor in the rat dorsal root ganglia (DRG). Results: Our results show that NONRATT021972 expression was significantly higher in the DRG of diabetes mellitus (DM) group compared with control group. NONRATT021972 expression in the DRG was reduced when DM rats were treated with NONRATT021972 siRNA. NONRATT021972 siRNA treatment in type 2 DM rats increased the mechanical withdrawal threshold (MWT), the thermal withdrawal latency (TWL) and the sensory nerve conduction velocity (SNCV) of rat tail nerves. After intravenous injection with NONRATT021972 siRNA in DM rats, the P2X
7 , GFAP and TNF-α expression levels in DRG were decreased. An interaction between the RNA (NONRATT021972) and protein (P2X7 ) was predicted by the application of bioinformatics technology. The BzATP-activated currents in DRG non-neurons (satellite glial cells) of DM rats were significantly increased compared to control rats. NONRATT021972 siRNA treatment inhibited the ATP-activated currents in HEK293 cells transfected with pEGFP-P2X7 . Conclusions: NONRATT021972 siRNA treatment can decrease the expression levels of P2X7 mRNA and protein and inhibit the activation of satellite glial cells (SGCs) in the DRG of type 2 DM rats. Moreover, NONRATT021972 siRNA treatment reduced the release of inflammatory factors (TNF-α), thereby inhibiting the excitability of DRG neurons and reducing mechanical and thermal hyperalgesia in type 2 DM rats. [ABSTRACT FROM AUTHOR]- Published
- 2016
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31. Synergism between the mTOR inhibitor rapamycin and FAK down-regulation in the treatment of acute lymphoblastic leukemia.
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Pei-Jie Shi, Lu-Hong Xu, Kang-Yu Lin, Wen-jun Weng, and Jian-Pei Fang
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LYMPHOBLASTIC leukemia diagnosis , *FOCAL adhesion kinase regulation , *RAPAMYCIN , *HEMATOLOGIC malignancies , *MACROLIDE antibiotics , *DRUG synergism , *THERAPEUTICS - Abstract
Background: Acute lymphoblastic leukemia (ALL) is an aggressive malignant disorder of lymphoid progenitor cells in both children and adults. Although improvements in contemporary therapy and development of new treatment strategies have led to dramatic increases in the cure rate in children with ALL, the relapse rate remains high and the prognosis of relapsed childhood ALL is poor. Molecularly targeted therapies have emerged as the leading treatments in cancer therapy. Multi-cytotoxic drug regimens have achieved success, yet many studies addressing targeted therapies have focused on only one single agent. In this study, we attempted to investigate whether the effect of the mammalian target of rapamycin (mTOR) inhibitor rapamycin is synergistic with the effect of focal adhesion kinase (FAK) down-regulation in the treatment of ALL. Methods: The effect of rapamycin combined with FAK down-regulation on cell proliferation, the cell cycle, and apoptosis was investigated in the human precursor B acute lymphoblastic leukemia cells REH and on survival time and leukemia progression in a non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mouse model. Results: When combined with FAK down-regulation, rapamycin-induced suppression of cell proliferation, G0/G1 cell cycle arrest, and apoptosis were significantly enhanced. In addition, REH cell-injected NOD/SCID mice treated with rapamycin and a short-hairpin RNA (shRNA) to down-regulate FAK had significantly longer survival times and slower leukemia progression compared with mice injected with REH-empty vector cells and treated with rapamycin. Moreover, the B-cell CLL/lymphoma-2 (BCL-2) gene family was shown to be involved in the enhancement, by combined treatment, of REH cell apoptosis. Conclusions: FAK down-regulation enhanced the in vitro and in vivo inhibitory effects of rapamycin on REH cell growth, indicating that the simultaneous targeting of mTOR- and FAK-related pathways might offer a novel and powerful strategy for treating ALL. [ABSTRACT FROM AUTHOR]
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- 2016
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32. Cancer incidence in patients with type 2 diabetes mellitus: a population-based cohort study in Shanghai.
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Hui-Lin Xu, Hong Fang, Wang-Hong Xu, Guo-You Qin, Yu-Jie Yan, Bao-Dong Yao, Nai-Qing Zhao, Yi-Nan Liu, Fen Zhang, Wei-Xi Li, Na Wang, Jie Zhou, Jin-Ling Zhang, Li-Yun Zhao, Lun-Qiang Li, Yan-Ping Zhao, Xu, Hui-Lin, Fang, Hong, Xu, Wang-Hong, and Qin, Guo-You
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DISEASE incidence ,CANCER risk factors ,TYPE 2 diabetes ,COHORT analysis ,HEALTH of Chinese people ,ELECTRONIC health records ,POISSON distribution ,TYPE 2 diabetes complications ,LONGITUDINAL method ,PUBLIC health surveillance ,TUMORS ,RELATIVE medical risk - Abstract
Background: Type 2 diabetes mellitus (T2DM) has been suggested to increase the risk of cancers. The aim of this study was to investigate the risk of common cancers in Chinese patients with T2DM.Methods: A population-based retrospective cohort study including 36,379 T2DM patients was conducted in Minhang District of Shanghai, China, during 2004 to 2010. All T2DM patients were enrolled from the standardized management system based on local electronic information system. Newly-diagnosed cancer cases were identified by record-linkage with the Shanghai Cancer Registry. Standardized incidence ratios (SIR) and 95% confidence interval (CI) were used to estimate the risk of cancers among T2DM patients.Results: Overall crude incidence rate (CIR) of cancers was 955.21 per 105 person-years in men and 829.57 per 105 person-years in women. Increased risk of cancer was found in both gender, with an SIR being 1.28 (95% CI = 1.17-1.38) in men and 1.44 (95% CI =1.32-1.55) in women. Increased risk of colon (SIR = 1.97; 95% CI = 1.49 to 2.46), rectum (1.72; 1.23 to 2.21), prostate (2.87; 2.19 to 3.56), and bladder cancers (1.98, 1.28 to 2.68) were observed in men and elevated risk of colon (1.67; 1.25 to 2.08), breast (1.66; 1.38 to 1.95), and corpus uteri cancers (2.87; 2.03 to 3.71) were observed in women.Conclusions: Our results indicate that Chinese patients with T2DM may have an increased risk of some cancers, and the increase may vary by sub-sites of cancers. [ABSTRACT FROM AUTHOR]- Published
- 2015
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33. Factors associated with seizure occurrence and long-term seizure control in pediatric brain arteriovenous malformation: a retrospective analysis of 89 patients.
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Shuang Liu, Hong-xu Chen, Qing Mao, Chao You, and Jian-guo Xu
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ARTERIOVENOUS malformation , *BLOOD-vessel abnormalities , *SPASMS , *SEIZURES (Medicine) , *LOGISTIC regression analysis - Abstract
Background: Few studies have examined seizures in pediatric brain arteriovenous malformation. In our study, risk factors associated with seizure occurrence and long-term seizure control outcomes after different treatments in pediatric arteriovenous malformation patients were investigated. Methods: A retrospective analysis was conducted with clinical data from a cohort of 89 pediatric brain arteriovenous malformation patients acquired between 2008 and 2013. Univariate and multivariate analyses were used to assess risk factors associated with seizure incidence. Patients who presented with seizure before treatment were evaluated using the Engel classification during the follow-up period. Results: A higher risk of seizure occurrence was observed in large size and unruptured brain arteriovenous malformations using multivariate logistic regression analysis (p < 0.05). A total of 22 children, who presented with seizure before the interventions, were included in subsequent analysis. During a mean follow-up period of 2.3 years after the intervention, 12 (55 %) of these children were classified as Engel class I after treatment. Conclusions: Seizures were the most common symptom in unruptured bAVMs. Size of the brain arteriovenous malformation is highly significant to seizure occurrence. Patients with cerebral hemorrhage are prone to having an acute seizure occurrence. The different therapies examined all improved seizure control to varying degrees. [ABSTRACT FROM AUTHOR]
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- 2015
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34. AXIN2 rs2240308 polymorphism contributes to increased cancer risk: evidence based on a meta-analysis.
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Zhitong Wu, Yifan Sun, Shifu Tang, Chunming Liu, Shengbo Zhu, Lili Wei, and Hong Xu
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CANCER risk factors ,GENETIC polymorphisms ,WNT genes ,GLYCOGEN synthase kinase ,ADENOMATOUS polyposis coli ,CONFIDENCE intervals ,META-analysis - Abstract
Background: Variants in the axis inhibition 2 (AXIN2) gene might alter the protein's structure or function or create a multiprotein destruction complex in the Wnt signaling pathway and thus affect an individual's susceptibility to cancer. The objective of this study is to evaluate broadly the evidence available for the AXIN2 rs2240308 polymorphism and risk of cancer. Methods: A comprehensive literature search was undertaken for eligible studies in Embase, PubMed, and Cochrane Library up to Nov 30, 2014. Odds ratios (ORs) and the corresponding 95 % confidence intervals (CIs) were used to measure the strength of the models. Results: Eight articles (10 case-control studies with 1,502 cases and 1,590 controls) were included in this analysis. Overall, the AXIN2 rs2240308 polymorphism was associated with a significant increase in the risk of cancer (G allele vs. A allele: OR = 1.21, 95 % CI = 1.05-1.40, I
2 = 39.5 % and PQ = 0.094 for heterogeneity; GG vs. AA: OR = 1.30, 95 % CI = 1.04-1.63, I2 = 35.9 % and PQ = 0.121 for heterogeneity; GG vs. GA + AA: OR = 1.36, 95 % CI = 1.17-1.58, I2 = 19.5 % and PQ = 0.263 for heterogeneity). Asian populations showed similar results. Stratified analysis by cancer types indicated that the AXIN2 rs2240308 polymorphism increases the risk of lung cancer (G allele vs. A allele: OR = 1.36, 95 % CI = 1.17-1.59; GA vs. AA: OR = 1.43, 95 % CI = 1.01-2.02; GG vs. AA: OR = 1.93, 95 % CI = 1.36-2.75; GG + GA vs. AA: OR = 1.65, 95 % CI = 1.18-2.30; GG vs. GA + AA: OR = 1.45, 95 % CI = 1.18-1.79. All I2 < 50 % and PQ > 0.100 for heterogeneity). Conclusions: This study showed that the AXIN2 rs2240308 polymorphism contribute to increasing the risk of cancer, especially lung cancer in Asian populations. [ABSTRACT FROM AUTHOR]- Published
- 2015
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35. A vital role for Angptl3 in the PAN-induced podocyte loss by affecting detachment and apoptosis in vitro.
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Rufeng Dai, Yi Lin, Haimei Liu, Jia Rao, Yihui Zhai, Xiliang Zha, Xiaoyan Fang, and Hong Xu
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APOPTOSIS ,CELL death ,APOPTIN ,F-actin ,ACTIN - Abstract
Background: Podocyte detachment and apoptosis are two risk factors causing podocyte loss, F-actin rearrangement is involved in detachment and apoptosis. However, the nature of events that promote detachment and apoptosis of podocytes and whether detachment occurred simultaneously with apoptosis are still unclear. Previously, it was found that angiopoietin-like3 (Angptl3) induces F-actin rearrangement in podocytes. In this study we investigate whether Angptl3 influences podocyte loss (detachment and apoptosis) and the process through which Angptl3 exactly influenced the podocyte loss. Methods: In conditionally immortalized mice podocytes, recombinant mice Angptl3 protein (rm-Angptl3) was used to mimic Angptl3 overexpression model and transfection with small interfering RNA (siRNA) to knockdown the expression of Angptl3. Both flow cytometry analysis and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay were used to detect apoptosis. Rearrangement of F-actin was assessed using confocal microscopy. Western blot assay was used to measure levels of Angptl3, integrin α3β1, integrin-linked kinase (ILK), p53, caspase 3, and phosphorylation of integrin β1. Results: In a puromycin aminonucleoside (PAN)-induced podocyte injury model, rm-Angptl3 accelerated the loss of podocytes, both detachment and apoptosis occurred, and F-actin rearrangement is involved in the process. However, knockdown of Angptl3 by siRNA markedly ameliorated these injuries. Observed effects were partially correlated with the altered integrin α3β1, ILK and p53, rather than caspase 3. Conclusions: Angptl3 is a novel factor involved in the PAN-induced podocyte loss by affecting detachment and apoptosis in vitro. This study helps to deepen the understanding of the mechanisms of podocyte loss and lays the foundation for developing a new successful therapy for podocyte injury via lower expression of Angptl3. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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36. YY1 suppresses FEN1 over-expression and drug resistance in breast cancer.
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Jianwei Wang, Lina Zhou, Zhi Li, Ting Zhang, Wenpeng Liu, Zheng Liu, Yate-Ching Yuan, Fan Su, Lu Xu, Yan Wang, Xiaotong Zhou, Hong Xu, Yuejin Hua, Ying-Jie Wang, Li Zheng, Yue-E Teng, and Binghui Shen
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BREAST cancer treatment ,TUMOR suppressor genes ,ENDONUCLEASE genetics ,GENETIC overexpression ,DRUG resistance in cancer cells ,DNA repair - Abstract
Background: Drug resistance is a major challenge in cancer therapeutics. Abundant evidence indicates that DNA repair systems are enhanced after repetitive chemotherapeutic treatments, rendering cancers cells drug-resistant. Flap endonuclease 1 (FEN1) plays critical roles in DNA replication and repair and in counteracting replication stress, which is a key mechanism for many chemotherapeutic drugs to kill cancer cells. FEN1 was previously shown to be upregulated in response to DNA damaging agents. However, it is unclear about the transcription factors that regulate FEN1 expression in human cancer. More importantly, it is unknown whether up-regulation of FEN1 has an adverse impact on the prognosis of chemotherapeutic treatments of human cancers. Methods: To reveal regulation mechanism of FEN1 expression, we search and identify FEN1 transcription factors or repressors and investigate their function on FEN1 expression by using a combination of biochemical, molecular, and cellular approaches. Furthermore, to gain insights into the impact of FEN1 levels on the response of human cancer to therapeutic treatments, we determine FEN1 levels in human breast cancer specimens and correlate them to the response to treatments and the survivorship of corresponding breast cancer patients. Results: We observe that FEN1 is significantly up-regulated upon treatment of chemotherapeutic drugs such as mitomycin C (MMC) and Taxol in breast cancer cells. We identify that the transcription factor/repressor YY1 binds to the FEN1 promoter and suppresses the expression of FEN1 gene. In response to the drug treatments, YY1 is dissociated from the FEN1 promoter region leading over-expression of FEN1. Overexpression of YY1 in the cells results in down-regulation of FEN1 and sensitization of the cancer cells to MMC or taxol. Furthermore, we observe that the level of FEN1 is inversely correlated with cancer drug and radiation resistance and with survivorship in breast cancer patients. Conclusion: Altogether, our current data indicate that YY1 is a transcription repressor of FEN1 regulating FEN1 levels in response to DNA damaging agents. FEN1 is up-regulated in human breast cancer and its levels inversely correlated with cancer drug and radiation resistance and with survivorship in breast cancer patients. [ABSTRACT FROM AUTHOR]
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- 2015
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37. Improved self-management skills in Chinese diabetes patients through a comprehensive health literacy strategy: study protocol of a cluster randomized controlled trial.
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Wang Hong Xu, Rothman, Russell L., Rui Li, Yingyao Chen, Qinghua Xia, Hong Fang, Junling Gao, Yujie Yan, Peng Zhou, Yu Jiang, Yinan Liu, Fangjia Zhou, Wei Wang, Minling Chen, Xiao Yu Liu, and Xiao Na Liu
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ASTHMA treatment , *DRUG therapy , *RANDOMIZED controlled trials , *DRUG utilization , *CLINICAL trials - Abstract
Background Diabetes self-management often involves the interpretation and application of oral, written, or quantitative information. Numerous diabetes patients in China have limited health literacy, which likely leads to poorer clinical outcomes. This study is designed to examine the efficacy and cost-effectiveness of addressing health literacy to improve self-management skills and glycemic control in Chinese diabetes patients. Methods/design This is a cluster randomized controlled trial (RCT) conducted in 20 community healthcare sites in Shanghai, China. Overall, 800 diabetes patients will be randomized into intervention and control arms and will have a baseline hemoglobin A1c (HbA1c) assay and undergo a baseline survey which includes measures of health literacy and diabetes numeracy using revised Chinese versions of the Health Literacy Management Scale and Diabetes Numeracy Test Scale. During the 1-year period of intervention, while the control group will receive usual care, the intervention group will be supplemented with a comprehensive health literacy strategy which includes i) training healthcare providers in effective health communication skills that address issues related to low literacy, and ii) use of an interactive Diabetes Education Toolkit to improve patient understanding and behaviors. Assessments will be conducted at both patient and healthcare provider levels, and will take place upon admission and after 3, 6, 12, and 24 months of intervention. The primary outcome will be the improvement in HbA1c between Intervention group and Control group patients. Secondary outcomes at the patient level will include improvement in i) clinical outcomes (blood pressure, fasting lipids, body mass index, weight, smoking status), ii) patient reported selfmanagement behaviors, and iii) patient-reported self-efficacy. . Outcomes at the provider level will include: i) provider satisfaction and ii) intensity and type of care provided. The effects of the intervention will be examined in multivariable general linear models. Both costeffectiveness and cost-utility analyses will be performed. Discussion The main strengths of this study are its large sample size and RCT design, involvement of both patients and healthcare providers, and the long term follow-up (24-months). This project will help to demonstrate the value of addressing health literacy and health communication to improve self-management and clinical outcomes among Chinese diabetes patients. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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38. Lasiodiplodia sp. ME4-2, an endophytic fungus from the floral parts of Viscum coloratum, produces indole-3-carboxylic acid and other aromatic metabolites.
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Chao-Dong Qian, Yu-Hang Fu, Fu-Sheng Jiang, Zheng-Hong Xu, Dong-Qing Cheng, Bin Ding, Cheng-Xian Gao, and Zhi-Shan Ding
- Abstract
Background: Studies on endophytes, a relatively under-explored group of microorganisms, are currently popular amongst biologists and natural product researchers. A fungal strain (ME4-2) was isolated from flower samples of mistletoe (Viscum coloratum) during a screening program for endophytes. As limited information on floral endophytes is available, the aim of the present study is to characterise fungal endophytes using their secondary metabolites. Results: ME4-2 grew well in both natural and basic synthetic media but produced no conidia. Sequence analysis of its internal transcribed spacer rDNA demonstrated that ME4-2 forms a distinct branch within the genus Lasiodiplodia and is closely related to L. pseudotheobromae. This floral endophyte was thus identified as Lasiodiplodia sp. based on its molecular biological characteristics. Five aromatic compounds, including cyclo-(Trp-Ala), indole-3-carboxylic acid (ICA), indole-3-carbaldehyde, mellein and 2-phenylethanol, were found in the culture. The structures of these compounds were determined using spectroscopic methods combined with gas chromatography. To the best of our knowledge, our work is the first to report isolation of these aromatic metabolites from a floral endophyte. Interestingly, ICA, a major secondary metabolite produced by ME4-2, seemed to be biosynthesized via an unusual pathway. Furthermore, our results indicate that the fungus ME4-2 is a potent producer of 2-phenylethanol, which is a common component of floral essential oils. Conclusions: This study introduces a fungal strain producing several important aromatic metabolites with pharmaceutical or food applications and suggests that endophytic fungi isolated from plant flowers are promising natural sources of aromatic compounds. [ABSTRACT FROM AUTHOR]
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- 2014
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39. MicroRNA-9 expression is a prognostic biomarker in patients with osteosarcoma.
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Shi-hong Xu, Yong-liang Yang, Shu-mei Han, and Zong-hui Wu
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MICRORNA , *BIOMARKERS , *OSTEOSARCOMA , *KAPLAN-Meier estimator , *MULTIVARIATE analysis , *PATIENTS - Abstract
Background The purpose of the present study was to examine the expression levels of microRNA-9 (miR- 9) in osteosarcoma tissues and normal bone tissues, and investigate the relationships between miR-9 expression, clinicopathological features and the prognosis of patients with osteosarcoma. Methods The expression levels of miR-9 in osteosarcoma tissues and corresponding non-cancerous tissues were detected using a real-time quantitative assay. Differences in patient survival were determined using the Kaplan-Meier method and a log-rank test. A Cox proportional hazards regression analysis was used for univariate and multivariate analyses of prognostic values. Results Compared to non-cancerous bone tissues, the expression levels of miR-9 in osteosarcoma tissues were significantly elevated (P < 0.001). We found that the expression level of miR-9 was significantly associated with tumor size (P = 0.011), clinical stage (P = 0.009) and distant metastasis (P < 0.001). The Kaplan-Meier curve showed that patients with low miR-9 expression survived significantly longer than patients with high miR-9 expression (P = 0.0017). Multivariate analysis suggested that miR-9 expression level (P = 0.002) is an independent prognostic factors for overall survival. Conclusions The findings of our study suggest that increased miR-9 expression has a strong correlation with the aggressive progression of osteosarcoma and its overexpression is a statistically significant risk factor affecting overall survival, suggesting that increased miR-9 expression could be a valuable marker of tumor progression and for prognosis of osteosarcoma. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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40. Activated microglia contribute to neuronal apoptosis in Toxoplasmic encephalitis.
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Yi-hua Zhang, He Chen, Ying Chen, Lu Wang, Yi-hong Cai, Min Li, Hui-qin Wen, Jian Du, Ran An, Qing-li Luo, Xue-long Wang, Zhao-Rong Lun, Yuan-hong Xu, and Ji-long Shen
- Abstract
Background: A plethora of evidence shows that activated microglia play a critical role in the pathogenesis of the central nervous system (CNS). Toxoplasmic encephalitis (TE) frequently occurs in HIV/AIDS patients. However, knowledge remains limited on the contributions of activated microglia to the pathogenesis of TE. Methods: A murine model of reactivated encephalitis was generated in a latent infection with Toxoplasma gondii induced by cyclophosphamide. The neuronal apoptosis in the CNS and the profile of pro-inflammatory cytokines were assayed in both in vitro and in vivo experiments. Results: Microglial cells were found to be activated in the cortex and hippocampus in the brain tissues of mice. The in vivo expression of interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and inducible nitric oxide synthase (iNOS) were up-regulated in TE mice, and accordingly, the neuronal apoptosis was significantly increased. The results were positively correlated with those of the in vitro experiments. Additionally,apoptosis of the mouse neuroblastoma type Neuro2a (N2a) remarkably increased when the N2a was co-cultured in transwell with microglial cells and Toxoplasma tachyzoites. Both in vivo and in vitro experiments showed that minocycline (a microglia inhibitor) treatment notably reduced microglial activation and neuronal apoptosis. Conclusions: Activated microglia contribute to neuronal apoptosis in TE and inhibition of microglia activation might represent a novel therapeutic strategy of TE. [ABSTRACT FROM AUTHOR]
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- 2014
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41. Mutational analysis of AGXT in two Chinese families with primary hyperoxaluria type 1.
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Guo-min Li, Hong Xu, Qian Shen, Yi-nv Gong, Xiao-yan Fang, Li Sun, Hai-mei Liu, and Yu An
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AUTOSOMAL recessive polycystic kidney ,ALANINE ,AMINOTRANSFERASES ,URINARY calculi ,KIDNEY calcification ,FAMILIES - Abstract
Background Primary hyperoxaluria type 1 is a rare autosomal recessive disease of glyoxylate metabolism caused by a defect in the liver-specific peroxisomal enzyme alanine:glyoxylate aminotransferase (AGT) that leads to hyperoxaluria, recurrent urolithiasis, and nephrocalcinosis. Methods Two unrelated patients with recurrent urolithiasis, along with members of their families, exhibited mutations in the AGXT gene by PCR direct sequencing. Results Two heterozygous mutations that predict truncated proteins, p.S81X and p.S275delinsRAfs, were identified in one patient. The p.S81X mutation is novel. Two heterozygous missense mutations, p.M1T and p.I202N, were detected in another patient but were not identified in her sibling. These four mutations were confirmed to be of paternal and maternal origin. Conclusions These are the first cases of primary hyperoxaluria type 1 to be diagnosed by clinical manifestations and AGXT gene mutations in mainland China. The novel p.S81X and p.I202N mutations detected in our study extend the spectrum of known AGXT gene mutations. [ABSTRACT FROM AUTHOR]
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- 2014
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42. Porcine circovirus type 2 in China: an update on and insights to its prevalence and control.
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Shao-Lun Zhai, Sheng-Nan Chen, Zhi-Hong Xu, Man-Hua Tang, Feng-Guo Wang, Xiao-Jing Li, Bei-Bei Sun, Su-Fang Deng, Jun Hu, Dian-Hong Lv, Xiao-Hui Wen, Jie Yuan, Man-Lin Luo, and Wen-Kang Wei
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CIRCOVIRUS diseases ,GENETIC polymorphisms ,VIRUS diseases ,INFECTIOUS disease transmission ,PUBLIC health - Abstract
Currently, porcine circovirus type 2 (PCV2) is considered the major pathogen of porcine circovirus associated-diseases (PCVAD) that causes large economic losses for the swine industry in the world annually, including China. Since the first report of PCV2 in 1998, it has been drawing tremendous attention for the government, farming enterprises, farmers, and veterinary practitioners. Chinese researchers have conducted a number of molecular epidemiological work on PCV2 by molecular approaches in the past several years, which has resulted in the identification of novel PCV2 genotypes and PCV2-like agents as well as the description of new prevalence patterns. Since late 2009, commercial PCV2 vaccines, including the subunit vaccines and inactivated vaccines, have already been used in Chinese swine farms. The aim of this review is to update the insights into the prevalence and control of PCV2 in China, which would contribute to understanding the epidemiology, control measures and design of novel vaccines for PCV2. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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43. Prevalence of chronic kidney disease across levels of glycemia among adults in Pudong New Area, Shanghai, China.
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Yi Zhou, Echouffo-Tcheugui, Justine B., Jian-jun Gu, Xiao-nan Ruan, Gen-ming Zhao, Wang-hong Xu, Li-ming Yang, Hong Zhang, Hua Qiu, Narayan, K. M. Venkat, and Qiao Sun
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KIDNEY diseases ,GLYCEMIC index ,BLOOD pressure ,CONFIDENCE intervals ,BODY mass index - Abstract
Background Few population-based studies have examined the relationship between glycemic status and chronic kidney disease (CKD) in China. We examined the prevalence of CKD across categories of glycemia [diagnosed diabetes, undiagnosed diabetes (fasting plasma glucose [FPG] ⩾ 126 mg/dL), prediabetes (FPG 100-126 mg/dL) and normal glycemia (FPG <100 mg/dL)] among Chinese adults and assessed the relative contribution of dysglycemia (prediabetes and/or diabetes) to the burden of CKD. Methods 5,584 Chinese adults aged 20-79 years were selected from the Pudong New Area of Shanghai through a multistage random sampling. Demographic and lifestyle characteristics, anthropometry and blood pressure were measured. Biochemical assays included FPG, serum creatinine and lipids, urinary creatinine and albumin. Prevalence of albuminuria [urine albumin-to-creatinine ratio (ACR) ⩾ 30 mg/g], decreased kidney function and CKD (either decreased kidney function or albuminuria) across levels of glycemia were estimated. Results The prevalence of albuminuria, decreased kidney function and CKD each increased with higher glycemic levels (P < 0.001). Based on the MDRD Study equation, the unadjusted CKD prevalence was 30.9%, 28.5%, 14.1% and 9.2% in those with diagnosed diabetes, undiagnosed diabetes, prediabetes and normoglycemia, respectively. The corresponding age-, gender- and hypertension-adjusted CKD prevalence were 25.8%, 25.0%, 12.3% and 9.1%, respectively. In a multivariable analysis, the factors associated with CKD were hypertension (Odds ratio [OR] 1.70, 95% confidence interval [CI]: 1.42-2.03), dysglycemia (OR 1.65, 95% CI: 1.39-1.95), female gender (OR 1.48, 95% CI: 1.25-1.75), higher triglycerides (OR 1.14, 95% CI: 1.08-1.20 per mmol/L), higher body mass index (OR 1.08, 95% CI: 1.05-1.10 per kg/m2), and older age (OR 1.02, 95% CI: 1.01 -1.03 per year). The population attributable risks (PARs) associated with diabetes, prediabetes, dysglycemia (diabetes and prediabetes) and hypertension were 18.4%, 19.7%, 30.3% and 44.5% for CKD as defined by the MDRD study equation, and 15.8%, 24.4%, 29.2% and 10.0% with the CKD-EPI equation. Estimates of prevalence and ORs of the relative contribution of various risk factors to CKD obtained with the CKD-EPI equation were similar. Conclusions As much as 30% of the CKD burden may be associated with dysglycemia among Chinese adults, independent of age, gender and hypertension status. Prevention and control of diabetes and prediabetes should be a high priority in reducing the CKD burden in China. [ABSTRACT FROM AUTHOR]
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- 2013
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44. Toxoplasma gondii prevalence in food animals and rodents in different regions of China: isolation, genotyping and mouse pathogenicity.
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Lin Wang, Hua-Wei Cheng, Kai-Quan Huang, Yuan-Hong Xu, Yong-Nian Li, Jian Du, Li Yu, Qing-Li Luo, Wei Wei, Ling Jiang, and Ji-Long Shen
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TOXOPLASMA gondii ,POLYMERASE chain reaction ,LABORATORY mice ,NUCLEOTIDE sequence ,INTRONS - Abstract
Background: Recent studies of Toxoplasma gondii isolates from animals in different regions of China have shown a limited genetic diversity and type China 1 was the dominant genotype of T. gondii prevalent in Chinese animals. However, little has been known concerning the isolation and genotyping of T. gondii circulating in chickens, pigs and rodents in China. The aim of the study was to characterize samples of T. gondii isolates obtained from naturally infected cats, pigs and free-range chickens slaughtered for human consumption in China. Methods: In the present study, brain tissues of 77 animals collected from different areas of China, including 24 free-range chickens (Gallus domesticus) , 13 voles (Rattus flavipectus), 23 pigs and 17 cats, were bioassayed in mice and viable T. gondii were isolated from the brains of eleven. These eleven T. gondii isolates were maintained in Kunming (KM) outbred mice and DNA isolated from tissues of infected mice was characterized using 11 PCR-restriction fragment length polymorphism (PCR-RFLP) markers: SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3. Moreover, to determine mouse virulence of China 1 lineage of parasites, a TgCtgy5 genotype isolate was selected randomly and assessed in KM mice with different inoculation doses. Results: Results of genotyping revealed that ten isolates were type China 1 (ToxoDB PCR-RFLP genotype #9), and TgCksz1 was a new genotype that was reported for the first time designated here as ToxoDB PCR-RFLP #225. No clonal types I, II and III lineages were found. DNA sequencing of four introns (EF1, HP2, UPRT1 and UPRT7) and two genes (GRA6 and GRA7) from representative isolates confirmed the results of PCR-RFLP genotyping. The TgCtgy5 isolate was highly virulent in KM mice; all infected mice died of acute toxoplasmosis, irrespective of the inoculation dose. The results indicate that mouse virulent isolates of T. gondii are predominantly circulating in cats in China. Conclusions: T. gondii isolated from chickens, pigs, cats and rodents in different locations in China were genotyped and the results reconfirmed the limited diversity of T. gondii in China and showed that type China 1 lineage was dominant in this country [ABSTRACT FROM AUTHOR]
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- 2013
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45. Effect of STAT5 silenced by siRNA on proliferation apoptosis and invasion of esophageal carcinoma cell line Eca-109.
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Qian Yang, Min Li, Tao Wang, Hong Xu, Wenqiao Zang, and Guoqiang Zhao
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SMALL interfering RNA ,ESOPHAGEAL cancer ,APOPTOSIS ,CANCER cells ,SQUAMOUS cell carcinoma - Abstract
Background: STAT is the backward position of cytokine and growth factor receptors in the nucleus, STAT dimers could bind to DNA and induce transcription of specific target genes. Several lines of evidence support the important roles of STAT, especially STAT5, in carcinogenesis. The overexpression of STAT 5 is related to the differentiation and apoptosis of tumor cells. However, the role of STAT5 in esophageal squamous cell carcinoma remains unclear. Methods: The siRNA vectors aiming to STAT5 gene were constructed. STAT5 siRNA was transfected into Eca-109 cells by Lipofectamine™2000. Expression of STAT5, Bcl-2 and Cyclin D1 were analyzed by Western blot and RT-PCR. Eca-109 cells proliferation was determined by MTT. Eca-109 cell cycle and apoptosis were detected by the flow cytometry. Boyden chamber was used to evaluate the invasion and metastasis capabilities of Eca-109 cells. Results: The double strands oligonucleotide of siRNA aiming to STAT5 was successfully cloned into the pRNAT-U6.1 vector, and the target sequence coincided with the design. RT-PCR and Western blotting detection demonstrated that the expression levels of STAT5, Bcl-2 and Cyclin D1 gene were obviously decreased in Eca-109 cells transfected with STAT5 siRNA. STAT5 siRNA could suppress the proliferation of Eca-109 cells. The proportion of S and G2/M period frequency was significantly decreased (p < 0.05). The proportion of G0/G1 period frequency was significantly increased (p < 0.05). The average amount of cells penetrating Matrigel was significantly decreased (p < 0.05). Conclusions: STAT5 silenced by siRNA could induce the apoptosis and suppress the proliferation, invasion and metastasis of esophageal carcinoma cell line Eca-109, which indicated STAT5 might be a novel therapeutic strategy for the human ESCC. [ABSTRACT FROM AUTHOR]
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- 2013
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46. RNAi targeting CXCR4 inhibits proliferation and invasion of esophageal carcinoma cells.
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Tao Wang, Yanfang Mi, Linping Pian, Ping Gao, Hong Xu, Yuling Zheng, and Xiaoyan Xuan
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RNA interference ,CXCR4 receptors ,ESOPHAGEAL cancer ,CANCER cell proliferation ,GENE therapy ,SMALL interfering RNA ,WESTERN immunoblotting - Abstract
CXC chemokine receptor 4 was found to be expressed by many different types of human cancers and its expression has been correlated with tumor aggressiveness, poor prognosis and resistance to chemotherapy. However the effect of CXCR4 on the esophageal carcinoma cells remains unclear, the present study explored the effects of CXCR4 siRNA on proliferation and invasion of esophageal carcinoma KYSE-150 and TE-13 cells. Two siRNA sequence targeting CXCR4 gene were constructed and then were transfected into KYSE-150 and TE-13 cells by Lipofectamine™2000. Changes of CXCR4 mRNA and protein were analyzed by qRT-PCR and Western blot. Effect of CXCR4 siRNA on KYSE-150 and TE-13 cells proliferation was determined by MTT. Transwell invasion assay was used to evaluate the invasion and metastasis of KYSE-150 and TE-13 cells. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice. qRT-PCR and Western blot demonstrate that the expression level of CXCR4 gene were obviously decreased in KYSE-150 and TE-13 cells transfected with CXCR4 targeting siRNA expression vectors. The average amount of cells transfected with CXCR4 siRNA penetrating Matrigel was significantly decreased (p<0.05). Injection of CXCR4 siRNA transfected cells inhibited tumor growth in a xenograft model compared with blank and negative control groups (p <0.05). CXCR4 silenced by siRNA could suppress the proliferation, invasion and metastasis of esophageal carcinoma cell lines KYSE-150 and TE-13 in vitro and in vivo. The results provide a theoretical and experimental basis for the gene therapy of ESCC using RNAi technology based on CXCR4 target site. [ABSTRACT FROM AUTHOR]
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- 2013
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47. Treatment with a copper-selective chelator causes substantive improvement in cardiac function of diabetic rats with left-ventricular impairment.
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Jun Lu, Pontré, Beau, Pickup, Stephen, Choong, Soon Y., Mingming Li, Hong Xu, Gamble, Gregory D., Phillips, Anthony R. J., Cowan, Brett R., Young, Alistair A., and Cooper, Garth J. S.
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IRON chelates ,LABORATORY rats ,HYPERTROPHY ,ACROMEGALY ,HYPERTROPHIC scars ,STREPTOZOTOCIN - Abstract
Background: Defective copper regulation is implicated as a causative mechanism of organ damage in diabetes. Treatment with trientine, a divalent-copper-selective chelator, improves arterial and renal structure/function in diabetes, wherein it also ameliorates left-ventricular (LV) hypertrophy. However, direct in vivo evidence that trientine can improve cardiac function in heart failure has hitherto been lacking. Methods: To determine whether trientine treatment could improve in vivo outcome, we measured cardiac function in groups of trientine-treated diabetic (TETA-DIA), non-drug-treated diabetic (DIA) and sham-treated control (SHAM) rats, by using in vivo high-field cardiac magnetic-resonance imaging (cMRI) and an ex vivo isolatedperfused working heart method. Forty age-matched animals underwent a cMRI scan after which 12 were randomized to the SHAM group and 28 underwent streptozotocin-injection; of these, 25 developed stable diabetes, and 12 were then randomized to receive no treatment for 16 weeks (DIA) and the other 13 to undergo 8-weeks' untreated diabetes followed by 8-weeks' drug treatment (TETA-DIA). Animals were studied again by cMRI at 8 and 16 weeks following disease induction, and finally by measurement of ex vivo cardiac function. Results: After eight weeks diabetes, rats (DIA/TETA-DIA) had developed significant impairment of LV function, as judged by impairment of ejection fraction (LVEF), cardiac output (CO), and LV mass (LVM)/body-mass (all P < 0.001), as well as other functional indexes. LVEF, CO (both P < 0.001) and the other indexes deteriorated further at 16 weeks in DIA, whereas trientine (TETA-DIA) improved cardiac function by elevating LVEF and CO (both P < 0.001), and also partially reversed the increase in LVM/body-mass (P < 0.05). In ex vivo hearts from DIA, the CO response to increasing preload pressure was deficient compared with SHAM (P < 0.001) whereas the preload-CO relationship was significantly improved in TETA-DIA animals (P < 0.001). Conclusions: Trientine treatment significantly improved cardiac function in diabetic rats with substantive LV impairment. These results implicate impaired copper regulation in the pathogenesis of impaired cardiac function caused by diabetic cardiomyopathy, and support ongoing studies of trientine treatment in patients with heart failure. [ABSTRACT FROM AUTHOR]
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- 2013
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48. Combining glycosylated hemoglobin A1c and fasting plasma glucose for diagnosis of type 2 diabetes in Chinese adults.
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Miao Mo, Weijian Zhong, Genming Zhao, Ye Ruan, Hua Zhang, Liang Shi, Dajiang Lu, Qundi Yang, Yanyun Li, Qingwu Jiang, Rui Li, and Wang-Hong Xu
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BLOOD sugar analysis ,TYPE 2 diabetes diagnosis ,MEDICAL screening ,ANALYSIS of variance ,ANTHROPOMETRY ,GLUCOSE tolerance tests ,GLYCOSYLATED hemoglobin ,RESEARCH funding ,STATISTICAL sampling ,STATISTICS ,DATA analysis ,CROSS-sectional method ,DATA analysis software ,DESCRIPTIVE statistics - Abstract
Background: Glycosylated hemoglobin A1c (HbA1c) has been applied to identify type 2 diabetes (T2DM) in the U.S. and European countries. It has not been used in China mainly due to lack of a standardized approach to measure HbA1c, short of knowledge about racial-specific standard and deficiency of an optimal cut-off point. Methods: To evaluate combination of HbA1c and fasting plasma glucose (FPG) in diagnosing T2DM in Chinese adults, a multistage sampling cross-sectional study was conducted in Shanghai, China, in 2009. The FPG measurement, HbA1c assay, and oral glucose tolerance test (OGTT) were performed in 6,661 Chinese adults (3057 men, 3604 women) who had no prior history of diabetes to identify unrecognized T2DM. Results: A total of 454 participants were identified as T2DM based on the 1999 World Health Organization (WHO) diagnostic criteria. Of these patients, 239 were detected using an FPG ≥ 7.0 mmol/l and 141 were further identified using an HbA1c ≥ 43 mmol/mol (6.1%), achieving a sensitivity of 83.7% and a specificity of 89.3% for combining use of FPG and HbA1c. In subjects at high risk of diabetes, the combining use of FPG and HbA1c produced a higher sensitivity and an improved positive predictive value (PPV), and had a satisfactory specificity and negative predictive value (NPV). Conclusions: The combining use of FPG and HbA1c is a potential screening and diagnosis approach for T2DM in Chinese adults, especially among those at high risk of the disease. [ABSTRACT FROM AUTHOR]
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- 2013
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49. Features of complementary and alternative medicine use by patients with coronary artery disease in Beijing: a cross-sectional study.
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Fu-Yong Chu, Xue Yan, Ze Zhang, Xing-Jiang Xiong, Jie Wang, and Hong-Xu Liu
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CORONARY heart disease treatment ,ALTERNATIVE medicine ,CHI-squared test ,CONFIDENCE intervals ,EPIDEMIOLOGY ,QUESTIONNAIRES ,RESEARCH funding ,T-test (Statistics) ,LOGISTIC regression analysis ,DATA analysis ,CROSS-sectional method ,DATA analysis software ,DESCRIPTIVE statistics - Abstract
Background: Complementary and alternative medicine (CAM) is commonly used in China for the management of coronary artery disease (CAD). However, few studies have been conducted to investigate the prevalence, perceived effectiveness, types, and reasons of CAM use in patients diagnosed with CAD. Methods: A cross-sectional study design was adopted. Questionnaires were distributed at the outpatient cardiac clinics of four tertiary-level teaching general hospitals in Beijing. Quantitative data were analyzed using Student's t-test. Categorical data were analyzed using chi-square test. Logistic regression was employed to explore factors associated with the use of CAM as well as CAM use features in Chinese medicine (CM) hospitals when significant differences were found upon comparisons. Results: From May to July, 2009, a total of 600 questionnaires were distributed, and 546 patients with a diagnosis of CAD responded with valid values and were included in the present study. CAM was used by 69.1% of the patients with CAD; the majority (75.9%) of these CAM users believes that CAM is effective. "Few side effects" (49.6%) was the main reason of CAM use; whereas "doubt of effect" (61.5%) was the main reason for non-use. Patent herbal medicine (90.7%) was the most commonly used CAM type. Compared with non-CAM users, CAM users tended to be older (p < 0.01), have a longer disease duration (p = 0.02) and better current health status. In addition, CAM users had significant lower odds for emergency admission and hospitalization within the past one year. Patients with CAD from CM and WM hospitals differ in CAM use frequency, types, perceived effectiveness, as well as reasons for CAM use or non-CAM use. Conclusion: The present study suggested a group of significant factors which could influence the use of CAM in patients with CAD. CAM use patterns differ in patients from CM and WM hospitals. [ABSTRACT FROM AUTHOR]
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- 2013
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50. Validation of the Oxford classification of IgA nephropathy for pediatric patients from China.
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Weibo Le, Cai-Hong Zeng, Zhangsuo Liu, Dong Liu, Qing Yang, Rui-Xia Lin, Zheng-Kun Xia, Zhong-Min Fan, Guanghua Zhu, Ying Wu, Hong Xu, Yihui Zhai, Ying Ding, Xiaoqing Yang, Shaoshan Liang, Hao Chen, Feng Xu, Qian Huang, Hongbing Shen, and Jianming Wang
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IGA glomerulonephritis ,PATIENTS ,PROGNOSIS ,TUBULOINTERSTITIAL nephritis & uveitis syndrome - Abstract
Background: The Oxford classification of IgA nephropathy (IgAN) provides a useful tool for prediction of renal prognosis. However, the application of this classification in children with IgAN needs validation in different patient populations. Methods: A total of 218 children with IgAN from 7 renal centers in China were enrolled. The inclusion criteria was similar to the original Oxford study. Results: There were 98 patients (45%) with mesangial proliferation (M1), 51 patients (23%) with endocapillary proliferation (E1), 136 patients (62%) with segmental sclerosis/adhesion lesion (S1), 13 patients (6%) with moderate tubulointerstitial fibrosis (T1 26-50% of cortex scarred), and only 2 patients (1%) with severe tubulointerstitial fibrosis(T2, >50% of cortex scarred). During a median follow-up duration of 56 months, 24 children (12.4%) developed ESRD or 50% decline in renal function. In univariate COX analysis, we found that tubular atrophy/interstitial fibrosis (HR 4.3, 95%CI 1.8-10.5, P < 0.001) and segmental glomerulosclerosis (HR 9.2 1.2-68.6, P = 0.03) were significant predictors of renal outcome. However, mesangial hypercellularity, endocapillary proliferation, crescents, and necrosis were not associated with renal prognosis. In the multivariate COX regression model, none of these pathologic lesions were shown to be independent risk factors of unfavorable renal outcome except for tubular atrophy/interstitial fibrosis (HR 2.9, 95%CI 1.0-7.9 P = 0.04).Conclusions: We confirmed tubular atrophy/interstitial fibrosis was the only feature independently associated with renal outcomes in Chinese children with IgAN. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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