1. Correction to: Genetic analysis of Wnt/PCP genes in neural tube defects
- Author
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Hongyan Wang, Ting Zhang, Rui Peng, Xuanye Cao, Zhongzhong Chen, Yihua Bao, Yunping Lei, Yufang Zheng, Fang Wang, and Richard H. Finnell
- Subjects
lcsh:Internal medicine ,lcsh:QH426-470 ,Computational biology ,Biology ,Genetic analysis ,Text mining ,Gene expression ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,Neural Tube Defects ,lcsh:RC31-1245 ,Child ,Gene ,Genetics (clinical) ,business.industry ,Neural tube ,Wnt signaling pathway ,Correction ,Cell Polarity ,Cadherins ,Human genetics ,Wnt Proteins ,lcsh:Genetics ,medicine.anatomical_structure ,HEK293 Cells ,Mutation ,DNA microarray ,business ,HeLa Cells - Abstract
Mouse homozygous mutants in Wnt/planar cell polarity (PCP) pathway genes have been shown to cause neural tube defects (NTDs) through the disruption of normal morphogenetic processes critical to neural tube closure (NTC). Knockout mice that are heterozygotes of single PCP genes likely fail to produce NTD phenotypes, yet damaging variants detected in human NTDs are almost always heterozygous, suggesting that other deleterious interacting variants are likely to be present. Nonetheless, the Wnt/PCP pathway remains a genetic hotspot. Addressing these issues is essential for understanding the genetic etiology of human NTDs.We performed targeted next-generation sequencing (NGS) on 30 NTD-predisposing Wnt/PCP pathway genes in 184 Chinese NTD cases. We subsequently replicated our findings for the CELSR1 gene in an independent cohort of 292 Caucasian NTD samples from the USA. Functional validations were confirmed using in vitro assays.CELSR1, CELSR2 and CELSR3 genes were significantly clustered with rare driver coding mutations (q-value 0.05) demonstrated by OncodriveCLUST. During the validation stage, the number of rare loss of function (LoF) variants in CELSR1 was significantly enriched in NTDs compared with the LoF counts in the ExAC database (p 0.001). Functional studies indicated compound heterozygote variants of CELSR2 p.Thr2026Met and DVL3 p.Asp403Asn result in down regulation of PCP signals.These data indicate rare damaging variants of the CELSR genes, identified in ~ 14% of NTD cases, are expected to be driver genes in the Wnt/PCP pathway. Compound damaging variants of CELSR genes and other Wnt/PCP genes, which were observed in 3.3% of the studied NTD cohort, are also expected to amplify these effects at the pathway level.
- Published
- 2021