1. shinyCurves, a shiny web application to analyse multisource qPCR amplification data: a COVID-19 case study.
- Author
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Olaechea-Lázaro S, García-Santisteban I, Pineda JR, Badiola I, Alonso S, Bilbao JR, and Fernandez-Jimenez N
- Subjects
- Data Analysis, Humans, Pandemics, Real-Time Polymerase Chain Reaction, SARS-CoV-2, COVID-19
- Abstract
Background: Quantitative, reverse transcription PCR (qRT-PCR) is currently the gold-standard for SARS-CoV-2 detection and it is also used for detection of other virus. Manual data analysis of a small number of qRT-PCR plates per day is a relatively simple task, but automated, integrative strategies are needed if a laboratory is dealing with hundreds of plates per day, as is being the case in the COVID-19 pandemic., Results: Here we present shinyCurves, an online shiny-based, free software to analyze qRT-PCR amplification data from multi-plate and multi-platform formats. Our shiny application does not require any programming experience and is able to call samples Positive, Negative or Undetermined for viral infection according to a number of user-defined settings, apart from providing a complete set of melting and amplification curve plots for the visual inspection of results., Conclusions: shinyCurves is a flexible, integrative and user-friendly software that speeds-up the analysis of massive qRT-PCR data from different sources, with the possibility of automatically producing and evaluating melting and amplification curve plots., (© 2021. The Author(s).)
- Published
- 2021
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