39 results on '"Jia, Qiang"'
Search Results
2. Fermented cordyceps powder alleviates silica-induced pulmonary inflammation and fibrosis in rats by regulating the Th immune response
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Pu, Shuangshuang, Yang, Zhifeng, Zhang, Xiaofeng, Li, Ming, Han, Na, Yang, Xiaohan, He, Jin, Yu, Gongchang, Meng, Xiangjing, Jia, Qiang, and Shao, Hua
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- 2023
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3. M6A transcriptome-wide map of circRNAs identified in the testis of normal and AZ-treated Xenopus laevis
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Zhang, Xin, Sai, Linlin, Zhang, Weiliang, Kan, Xingzheng, Jia, Qiang, Bo, Cunxiang, Yin, Wenhui, Shao, Hua, Han, Mingming, and Peng, Cheng
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- 2023
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4. α7 Nicotinic acetylcholine receptor: a key receptor in the cholinergic anti-inflammatory pathway exerting an antidepressant effect
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Liu, Huiyang, Zhang, Xiaomei, Shi, Peng, Yuan, Jiyuan, Jia, Qiang, Pi, Chao, Chen, Tao, Xiong, Linjin, Chen, Jinglin, Tang, Jia, Yue, Ruxu, Liu, Zerong, Shen, Hongping, Zuo, Ying, Wei, Yumeng, and Zhao, Ling
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- 2023
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5. The application of extracellular vesicles in colorectal cancer metastasis and drug resistance: recent advances and trends
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Xiong, Linjin, Wei, Yumeng, Jia, Qiang, Chen, Jinglin, Chen, Tao, Yuan, Jiyuan, Pi, Chao, Liu, Huiyang, Tang, Jia, Yin, Suyu, Zuo, Ying, Zhang, Xiaomei, Liu, Furong, Yang, Hongru, and Zhao, Ling
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- 2023
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6. Selective anti-tumor activity of glutathione-responsive abasic site trapping agent in anaplastic thyroid carcinoma
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Chai, Jinyan, Su, Mengxue, Zhang, Ruiguo, Li, Ning, Jia, Yuanyuan, Zheng, Wei, Tan, Jian, Jia, Qiang, Sun, Huabing, and Meng, Zhaowei
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- 2024
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7. Simvastatin attenuates silica-induced pulmonary inflammation and fibrosis in rats via the AMPK-NOX pathway
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Bo, Cunxiang, Liu, Fang, Zhang, Zewen, Du, Zhongjun, Xiu, Haidi, Zhang, Zhenling, Li, Ming, Zhang, Caiqing, and Jia, Qiang
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- 2024
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8. Synovium microenvironment-responsive injectable hydrogel inducing modulation of macrophages and elimination of synovial fibroblasts for enhanced treatment of rheumatoid arthritis
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Wu, Yiqun, Ge, Yu, Wang, Zhongshi, Zhu, Ying, Tian, Tianli, Wei, Jun, Jin, Yu, Zhao, Yi, jia, Qiang, Wu, Jun, and Ge, Liang
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- 2024
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9. Prognostic and predictive value of super-enhancer-derived signatures for survival and lung metastasis in osteosarcoma
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Huang, Guanyu, Zhang, Xuelin, Xu, Yu, Chen, Shuo, Cao, Qinghua, Liu, Weihai, Fu, Yiwei, Jia, Qiang, Shen, Jingnan, Yin, Junqiang, and Zhang, Jiajun
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- 2024
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10. Engineering a HEK-293T exosome-based delivery platform for efficient tumor-targeting chemotherapy/internal irradiation combination therapy
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Wang, Congcong, Li, Ning, Li, Yutian, Hou, Shasha, Zhang, Wenxin, Meng, Zhaowei, Wang, Shen, Jia, Qiang, Tan, Jian, Wang, Renfei, and Zhang, Ruiguo
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- 2022
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11. Integrative transcriptomic and proteomic analysis reveals mechanisms of silica-induced pulmonary fibrosis in rats
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Bo, Cunxiang, Zhang, Juan, Sai, Linlin, Du, Zhongjun, Yu, Gongchang, Li, Chao, Li, Ming, Peng, Cheng, Jia, Qiang, and Shao, Hua
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- 2022
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12. Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis
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Qi, Xuejie, Geng, Xiao, Zhang, Juan, Qu, Binpeng, Zhang, Xin, Jia, Qiang, Yin, Wenhui, Bo, Cunxiang, Liu, Yan, Li, Hao, Sai, Linlin, Han, Mingming, and Peng, Cheng
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- 2021
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13. Reduced intensity of early intensification does not increase the risk of relapse in children with standard risk acute lymphoblastic leukemia - a multi-centric clinical study of GD-2008-ALL protocol
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Li, Xin-Yu, Li, Jia-Qiang, Luo, Xue-Qun, Wu, Xue-Dong, Sun, Xin, Xu, Hong-Gui, Li, Chang-Gang, Liu, Ri-Yang, Sun, Xiao-Fei, Chen, Hui-Qin, Lin, Yu-Deng, LI, Chi-kong, and Fang, Jian-Pei
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- 2021
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14. Dynamic assessing silica particle-induced pulmonary fibrosis and associated regulation of long non-coding RNA expression in Wistar rats
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Sai, Linlin, Qi, Xuejie, Yu, Gongchang, Zhang, Juan, Zheng, Yuxin, Jia, Qiang, and Peng, Cheng
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- 2021
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15. CLK1/SRSF5 pathway induces aberrant exon skipping of METTL14 and Cyclin L2 and promotes growth and metastasis of pancreatic cancer
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Chen, Shi, Yang, Can, Wang, Zu-Wei, Hu, Jian-Fei, Pan, Jing-Jing, Liao, Cheng-Yu, Zhang, Jia-Qiang, Chen, Jiang-Zhi, Huang, Yi, Huang, Long, Zhan, Qian, Tian, Yi-Feng, Shen, Bai-Yong, and Wang, Yao-Dong
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- 2021
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16. Protocol for a prospective, cluster randomized trial to evaluate routine and deferred dialysis initiation (RADDI) in Chinese population
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Zhao, Xinju, Wang, Pei, Wang, Lining, Chen, Xiaonong, Huang, Wen, Mao, Yonghui, Hu, Rihong, Cheng, Xiaohong, Wang, Caili, Wang, Li, Zhang, Ping, Li, Detian, Wang, Yuzhu, Ye, Wenling, Chen, Yuqing, Jia, Qiang, Yan, Xiaoyan, and Zuo, Li
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- 2019
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17. M6A transcriptome-wide map of circRNAs identified in the testis of normal and AZ-treated Xenopus laevis.
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Zhang, Xin, Sai, Linlin, Zhang, Weiliang, Kan, Xingzheng, Jia, Qiang, Bo, Cunxiang, Yin, Wenhui, Shao, Hua, Han, Mingming, and Peng, Cheng
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XENOPUS laevis ,TESTIS ,TESTIS development ,GONADS ,ATRAZINE ,SPERMATOGENESIS ,RNA methylation - Abstract
Background: Evidence showed that N
6 -methyladenosine (m6 A) is strongly associated with male germline development. However, the role of m6 A methylation on circRNAs in amphibians remains unknown. In this study, we conducted m6 A sequencing analysis to explore the m6 A transcriptome-wide profile of circRNAs in testis tissues of Xenopus laevis (X. laevis) with and without treatment with 100 µg/L atrazine (AZ). Results: The analysis showed that m6 A modification of circRNAs enriched in sense overlapping in testes of X. laevis. We identified the differential m6 A modification sites within circRNAs in testes of AZ-exposed X. laevis and compared that with animals from control group. The results showed that a total of 1507 methylated m6 A sites was induced by AZ (760 up-methylated and 747 down-methylated). The cross-analysis exhibited a negative correlation of differentially methylated m6 A peaks and circRNAs expression level. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that 20 key pathways may be involved in the mechanism of testis damage of AZ-exposed X. laevis. Conclusions: These findings indicated that differentially m6 A-methylated circRNAs may play important roles in abnormal testis development of AZ-exposed X. laevis. This study is the first report about a map of m6 A modification of circRNAs in male X. laevis and provides a basis for further studying on the function and mechanism of m6 A methylation of circRNAs in the testis development of amphibian. [ABSTRACT FROM AUTHOR]- Published
- 2023
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18. Establishment and validation of an AI-aid method in the diagnosis of myocardial perfusion imaging.
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Zhang, Ruyi, Wang, Peng, Bian, Yanzhu, Fan, Yan, Li, Jianming, Liu, Xuehui, Shen, Jie, Hu, Yujing, Liao, Xianghe, Wang, He, Song, Chengyu, Li, Wangxiao, Wang, Xiaojie, Sun, Momo, Zhang, Jianping, Wang, Miao, Wang, Shen, Shen, Yiming, Zhang, Xuemei, and Jia, Qiang
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MYOCARDIAL perfusion imaging ,DIAGNOSIS methods ,CORONARY artery disease ,ARTIFICIAL intelligence ,COLLIMATORS - Abstract
Background: This study aimed to develop and validate an AI (artificial intelligence)-aid method in myocardial perfusion imaging (MPI) to differentiate ischemia in coronary artery disease. Methods: We retrospectively selected 599 patients who had received gated-MPI protocol. Images were acquired using hybrid SPECT-CT systems. A training set was used to train and develop the neural network and a validation set was used to test the predictive ability of the neural network. We used a learning technique named "YOLO" to carry out the training process. We compared the predictive accuracy of AI with that of physician interpreters (beginner, inexperienced, and experienced interpreters). Results: Training performance showed that the accuracy ranged from 66.20% to 94.64%, the recall rate ranged from 76.96% to 98.76%, and the average precision ranged from 80.17% to 98.15%. In the ROC analysis of the validation set, the sensitivity range was 88.9 ~ 93.8%, the specificity range was 93.0 ~ 97.6%, and the AUC range was 94.1 ~ 96.1%. In the comparison between AI and different interpreters, AI outperformed the other interpreters (most P-value < 0.05). Conclusion: The AI system of our study showed excellent predictive accuracy in the diagnosis of MPI protocols, and therefore might be potentially helpful to aid radiologists in clinical practice and develop more sophisticated models. [ABSTRACT FROM AUTHOR]
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- 2023
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19. A potential distribution map of wintering Swan Goose (Anser cygnoides) in the middle and lower Yangtze River floodplain, China
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An, An, Zhang, Yong, Cao, Lei, Jia, Qiang, and Wang, Xin
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- 2018
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20. Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis.
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Qi, Xuejie, Geng, Xiao, Zhang, Juan, Qu, Binpeng, Zhang, Xin, Jia, Qiang, Yin, Wenhui, Bo, Cunxiang, Liu, Yan, Li, Hao, Sai, Linlin, Han, Mingming, and Peng, Cheng
- Subjects
XENOPUS laevis ,ENDOCRINE disruptors ,TESTIS development ,TESTIS ,ATRAZINE - Abstract
Background: Increasing evidence suggested N
6 -methyladenosine (m6 A) modification is crucial for male germline development. However, m6 A modification of lncRNAs gains a little attention in amphibians in recent years. Xenopus laevis (X. laevis) was chosen to be an ideal model organism for testing environmental endocrine disrupting chemicals (EDCs) exposure and resultant effects. Atrazine (AZ) as an endocrine disrupt can effect development of testis in amphibians. Our previous study revealed that m6 A is a highly conserved modification across the species. Results: The results of m6 A sequences showed that m6 A-methylated lncRNAs enriched in intergenic region in testes of X. laevis. We further examined the differential expression of lncRNAs m6 A sites in testes of AZ-exposed and compared with that in animals from control group. The results indicated that up to 198 differentially methylated m6 A sites were detected within 188 lncRNAs, in which 89 significantly up-methylated sites and 109 significantly down-methylated sites. Data from KEGG pathway analysis indicated that AZ-affected lncRNAs m6 A sites were mainly involved in 10 pathways in which 3 mutual pathways were found in the result of differentially m6 A-methylated mRNAs. Conclusions: These findings suggested that differentially m6 A-methylated lncRNAs and these 3 pathways may act on regulatory roles in abnormal testis development of AZ-exposed X. laevis. This study for the first time provides insights into the profile of lncRNAs m6 A modifications in amphibian species. [ABSTRACT FROM AUTHOR]- Published
- 2021
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21. Lessons on malaria control in the ethnic minority regions in Northern Myanmar along the China border, 2007–2014
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Jia-Qiang Dong, Qing-Pu Li, Guang-Yun Zhang, Xiao-Ying Sun, Yang-Hui Tian, Jia-Yin Li, Jun Zhang, Yao Zhang, Ru-Bo Wang, Tao Cai, Qing-feng Zhang, Xiao-Yu Xu, and Zhi-Gui Xia
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,Malaria control ,030231 tropical medicine ,Psychological intervention ,Ethnic group ,Myanmar ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Acquired immunodeficiency syndrome (AIDS) ,Ethnic minority regions ,Environmental health ,parasitic diseases ,medicine ,Ethnicity ,Prevalence ,Humans ,030212 general & internal medicine ,Health Education ,Aged ,Aged, 80 and over ,Traditional medicine ,business.industry ,Public health ,Incidence (epidemiology) ,Incidence ,Public Health, Environmental and Occupational Health ,General Medicine ,Middle Aged ,medicine.disease ,Malaria ,Infectious Diseases ,Cross-Sectional Studies ,Northern Myanmar ,Tropical medicine ,Health education ,Female ,business ,Research Article - Abstract
Background For many countries where malaria is endemic, the burden of malaria is high in border regions. In ethnic minority areas along the Myanmar-China border, residents have poor access to medical care for diagnosis and treatment, and there have been many malaria outbreaks in such areas. Since 2007, with the support of the Global Fund to Fight AIDS, Tuberculosis and Malaria (GFATM), a malaria control project was introduced to reduce the malaria burden in several ethnic minority regions. Methods A malaria control network was established during the period from 2007 to 2014. Multiple malaria interventions, including diagnosis, treatment, distribution of LLINs and health education, were conducted to improve the accessibility and quality of malaria control services for local residents. Annual cross-sectional surveys were conducted to evaluate intervention coverage and indicators of malaria transmission. Results In ethnic minority regions where a malaria control network was established, both the annual malaria incidence (19.1 per thousand per year, in 2009; 8.7, in 2014) and malaria prevalence (13.6 % in 2008; 0.43 % in 2014) decreased dramatically during the past 5–6 years. A total of 851 393 febrile patients were detected, 202 598 malaria cases (including confirmed cases and suspected cases) were treated, and 759 574 LLINs were delivered to populations at risk. Of households in 2012, 73.9 % had at least one ITNs/LLINs (vs. 28.3 %, in 2008), and 50.7 % of children less than 5 years and 50.3 % of pregnant women slept under LLINs the night prior to their visit. Additionally, malaria knowledge was improved in 68.4 % of residents. Conclusion There has been great success in improving malaria control in these regions from 2007 to 2014. Malaria burdens have decreased, especially in KOK and WA. The continued maintenance of sustainable malaria control networks in these regions may be a long-term process, due to regional conflicts and the lack of funds, technology, and health workers. Furthermore, information and scientific support from the international community should be offered to these ethnic minority regions to uphold recent achievements. Electronic supplementary material The online version of this article (doi:10.1186/s40249-016-0191-0) contains supplementary material, which is available to authorized users.
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- 2016
22. Vaccine resistant pseudorabies virus causes mink infection in China.
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Wang, Gui-sheng, Du, Yijun, Wu, Jia-qiang, Tian, Fu-lin, Yu, Xue-jie, and Wang, Jin-bao
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DRUG resistance in bacteria ,AUJESZKY'S disease virus ,VIRAL diseases in minks ,VACCINES - Abstract
Background: Pseudorabies, a highly contagious infectious disease of swine is caused by pseudorabies virus (PRV). PRV can cause fatal infection in other animal species. Results: We report a deadly outbreak of pseudorabies that killed 87.2% (3522/4028) minks in a farm in 2014 in Shandong Province, China. PRV was isolated by using Vero cell culture and detected in mink samples by PCR from minks died during the outbreak. Epidemiological analysis indicated that 5.8% of minks (33/566) were PCR positive to PRV in Shandong Province. Phylogenetic analysis indicated that the PRV strains isolated from minks in this study were in the same clade with the Chinese porcine PRV isolates, which are resistant to the PRV vaccine. Conclusions: We demonstrated that pseudorabies virus caused an outbreak of minks in a farm in Shandong Province of China and the virus has a very high infection rate in minks in Shandong Province, which is a challenge for the fur industry in China. [ABSTRACT FROM AUTHOR]
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- 2018
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23. Identification of genome-wide SNP-SNP interactions associated with important traits in chicken.
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Hui Zhang, Jia-Qiang Yu, Li-Li Yang, Kramer, Luke M., Xin-Yang Zhang, Wei Na, Reecy, James M., and Hui Li
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SINGLE nucleotide polymorphisms , *GENETIC polymorphisms , *PHENOTYPES , *PERSONALITY , *CHEMOKINE genetics , *CYTOKINE genetics - Abstract
Background: In addition to additive genetic effects, epistatic interactions can play key roles in the control of phenotypic variation of traits of interest. In the current study, 475 male birds from lean and fat chicken lines were utilized as a resource population to detect significant epistatic effects associated with growth and carcass traits. Results: A total of 421 significant epistatic effects were associated with testis weight (TeW), from which 11 sub-networks (Sub-network1 to Sub-network11) were constructed. In Sub-network1, which was the biggest network, there was an interaction between GGA21 and GGAZ. Three genes on GGA21 (SDHB, PARK7 and VAMP3) and nine genes (AGTPBP1, CAMK4, CDC14B, FANCC, FBP1, GNAQ, PTCH1, ROR2 and STARD4) on GGAZ that might be potentially important candidate genes for testis growth and development were detected based on the annotated gene function. In Sub-network2, there was a SNP on GGA19 that interacted with 8 SNPs located on GGA10. The SNP (Gga_rs15834332) on GGA19 was located between C-C motif chemokine ligand 5 (CCL5) and MIR142. There were 32 Refgenes on GGA10, including TCF12 which is predicted to be a target gene of miR-142-5p. We hypothesize that miR-142-5p and TCF12 may interact with one another to regulate testis growth and development. Two genes (CDH12 and WNT8A) in the same cadherin signaling pathway were implicated as potentially important genes in the control of metatarsus circumference (MeC). There were no significant epistatic effects identified for the other carcass and growth traits, e.g. heart weight (HW), liver weight (LW), spleen weight (SW), muscular and glandular stomach weight (MGSW), carcass weight (CW), body weight (BW1, BW3, BW5, BW7), chest width (ChWi), metatarsus length (MeL). Conclusions: The results of the current study are helpful to better understand the genetic basis of carcass and growth traits, especially for testis growth and development in broilers. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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24. A novel swine model for evaluation of dyslipidemia and atherosclerosis induced by human CETP overexpression.
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Tao Chen, Meng Sun, Jia-Qiang Wang, Jin-Jin Cui, Zhong-Hua Liu, and Bo Yu
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DYSLIPIDEMIA ,ATHEROSCLEROSIS ,GENETIC overexpression ,CHOLESTERYL ester transfer protein ,FIBROBLASTS ,BIOMARKERS - Abstract
Background: The mechanism of cholesteryl ester transfer protein (CETP) in lipid metabolism is still unclear. Furthermore, the relationship of CETP and atherosclerosis (AS) has been controversial. As pigs are a good model for both lipid and AS research, we investigated the lipid metabolism of human CETP (hCETP) transgenic pigs and explored the mechanism of CETP in lipid modulation. Methods: Plasmids expressing the hCETP gene were designed, successfully constructed, and transfected into porcine fetal fibroblasts by liposomes. Using somatic cell nuclear transfer technology and embryonic transfer, hCETP transgenic pigs were generated. After the DNA, RNA, and protein levels were identified, positive hCETP transgenic pigs were selected. Blood samples were collected at different ages to evaluate the phenotypes of biochemical markers, and the metabolomes of plasma samples were analyzed by liquid mass spectrometry. Results: Eight positive hCETP transgenic pigs and five negative cloned pigs were generated by transgenic technology. Finally, five hCETP transgenic and five cloned pigs were grown healthily. After feeding with a normal diet, hCETP transgenic pigs compared with unmodified pigs had no significant differences in body weight, liver function, kidney function, or plasma ions, while total cholesterol and low-density lipoprotein were higher than in unmodified pigs, and high-density lipoprotein was significantly decreased. Metabolomics analysis showed that there were differences in metabolic components between hCETP transgenic pigs, cloned pigs, and unmodified pigs. Conclusions: In this study, we created hCETP transgenic pigs that could serve as an excellent model for lipid disorders and atherosclerosis. [ABSTRACT FROM AUTHOR]
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- 2017
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25. Serum selenium level and gestational diabetes mellitus: a systematic review and meta-analysis.
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Fei-Juan Kong, Lei-Lei Ma, Shu-Ping Chen, Ge Li, and Jia-Qiang Zhou
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GESTATIONAL diabetes ,SELENIUM in the body ,BLOOD serum analysis ,SEX factors in disease ,HEALTH & race - Abstract
Background: The association between serum selenium level and gestational diabetes mellitus (GDM) is controversial. The aim of our study was to systematically review available literature linking selenium to GDM for a comprehensive understanding of the relationship between serum selenium level and GDM in human. Methods: PubMed, The Cochrane Library and Medline were searched for studies published up to August 2016. Manual searches of references of the relevant original studies were carried out. Pooled estimates were measured using the fixed or random effect model. Overall effect was reported in a standard mean difference (SMD). All data were analyzed with Review Manager 5.3 and Stata 12.0. Results: Of 44 references reviewed, seven studies involving 569 patients met our inclusion criteria and contributed to meta-analysis. All the studies were used to evaluate the relationship between serum selenium level and GDM. Selenium level was significantly lower in women with GDM than those without GDM (SMD = -1.17; 95% CI: -1.98 to -0.35, P = 0.005). Subgroup analysis showed that such trend was consistent within the non-Caucasian population (Asia: SMD = -2.82; 95% CI: -5.21 to -0.43, P = 0.02; Africa: SMD = -0.56; 95% CI: -1.07 to -0.05, P = 0.03) and in the third trimester (SMD = -1.78; 95% CI: -3.04 to -0.52, P = 0.006), but not within the Caucasian population (Europe: SMD = -0.6; 95% CI: -1.98 to 0.78, P = 0.39) or in the second trimester (SMD = -0.68; 95% CI: -1.6 to 0.25, P = 0.15). Conclusions: The available evidences suggested that serum selenium level was lower in women with GDM than those with normal glucose tolerance, especially within the non-Caucasian population and in the third trimester. However, well-designed prospective studies are needed to understand dynamic associations between selenium status and GDM risk. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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26. Lessons on malaria control in the ethnic minority regions in Northern Myanmar along the China border, 2007-2014.
- Author
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Ru-Bo Wang, Jia-Qiang Dong, Zhi-Gui Xia, Tao Cai, Qing-Feng Zhang, Yao Zhang, Yang-Hui Tian, Xiao-Ying Sun, Guang-Yun Zhang, Qing-Pu Li, Xiao-Yu Xu, Jia-Yin Li, and Jun Zhang
- Subjects
- *
MALARIA prevention , *MALARIA diagnosis , *MALARIA treatment , *DISEASE prevalence - Abstract
Background: For many countries where malaria is endemic, the burden of malaria is high in border regions. In ethnic minority areas along the Myanmar-China border, residents have poor access to medical care for diagnosis and treatment, and there have been many malaria outbreaks in such areas. Since 2007, with the support of the Global Fund to Fight AIDS, Tuberculosis and Malaria (GFATM), a malaria control project was introduced to reduce the malaria burden in several ethnic minority regions. Methods: A malaria control network was established during the period from 2007 to 2014. Multiple malaria interventions, including diagnosis, treatment, distribution of LLINs and health education, were conducted to improve the accessibility and quality of malaria control services for local residents. Annual cross-sectional surveys were conducted to evaluate intervention coverage and indicators of malaria transmission. Results: In ethnic minority regions where a malaria control network was established, both the annual malaria incidence (19.1 per thousand per year, in 2009; 8.7, in 2014) and malaria prevalence (13.6 % in 2008; 0.43 % in 2014) decreased dramatically during the past 5-6 years. A total of 851 393 febrile patients were detected, 202 598 malaria cases (including confirmed cases and suspected cases) were treated, and 759 574 LLINs were delivered to populations at risk. Of households in 2012, 73.9 % had at least one ITNs/LLINs (vs. 28.3 %, in 2008), and 50.7 % of children less than 5 years and 50.3 % of pregnant women slept under LLINs the night prior to their visit. Additionally, malaria knowledge was improved in 68.4 % of residents. Conclusion: There has been great success in improving malaria control in these regions from 2007 to 2014. Malaria burdens have decreased, especially in KOK and WA. The continued maintenance of sustainable malaria control networks in these regions may be a long-term process, due to regional conflicts and the lack of funds, technology, and health workers. Furthermore, information and scientific support from the international community should be offered to these ethnic minority regions to uphold recent achievements. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
27. Effectiveness and impact of the crossborder healthcare model as implemented by non-governmental organizations: case study of the malaria control programs by health poverty action on the China- Myanmar border.
- Author
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Jun Zhang, Jia-Qiang Dong, Jia-Ying Li, Yue Zhang, Yang-Hui Tian, Xiao-Ying Sun, Guang-Yun Zhang, Qing-Pu Li, Xiao-Yu Xu, and Tao Cai
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MALARIA prevention , *MEDICAL care , *NONGOVERNMENTAL organizations - Abstract
Background: In the Yunnan province of China, 18 counties in six prefectures border Myanmar. Due to its particular combination of geographic features, climate conditions, and cultural landscape, the area provides a suitable environment for the spread of insect-borne diseases such as malaria. In five identified Myanmar Special Regions along the China-Myanmar border, economic development is lagging, people live in extreme poverty, and the healthcare system is fragile. Coupled with political and other reasons, this precludes malaria control work to be effectively carried out in Myanmar, resulting in a heavy burden of the disease. Frequent population movements and favorable conditions for malaria transmission on the border fuel difficulties in controlling and eliminating the spread of the disease in the area. Case presentation: To reduce the prevalence of malaria in the China-Myanmar border area and improve healthcare services for local residents in this particular environment, Health Poverty Action (HPA) has provided malaria aid in the area since the beginning of 2006, as a sub-recipient of the China Global Fund Malaria Programs. In this case study, we examined HPA's activities as part of its malaria control programs in the area, analyzed and summarized the effectiveness and impact of the cross-border healthcare model as implemented by non-governmental organizations, and put forward suggestions for cross-border health aid models and for the prevention of malaria transmission in the Greater Mekong Subregion. Conclusions: HPA had carried out a great quantity of successful malaria control activities in border areas between China and Myanmar, strengthened the partnership and established the collaboration, coordination and cooperation channels among stakeholders. HPA has laid good groundwork and developed its valuable model that could be highlighted and referenced. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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28. Detection of genome-wide copy number variations in two chicken lines divergently selected for abdominal fat content.
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Hui Zhang, Zhi-Qiang Du, Jia-Qiang Dong, Hai-Xia Wang, Hong-Yan Shi, Ning Wang, Shou-Zhi Wang, and Hui Li
- Subjects
GENOMES ,DETECTION of microorganisms ,CHICKENS ,ANIMAL models in research ,SLOW potentials (Electrophysiology) ,ABDOMINAL adipose tissue ,ALGORITHMS - Abstract
Background The chicken (Gallus gallus) is an important model organism that bridges the evolutionary gap between mammals and other vertebrates. Copy number variations (CNVs) are a form of genomic structural variation widely distributed in the genome. CNV analysis has recently gained greater attention and momentum, as the identification of CNVs can contribute to a better understanding of traits important to both humans and other animals. To detect chicken CNVs, we genotyped 475 animals derived from two broiler chicken lines divergently selected for abdominal fat content using chicken 60 K SNP array, which is a high-throughput method widely used in chicken genomics studies. Results Using PennCNV algorithm, we detected 438 and 291 CNVs in the lean and fat lines, respectively, corresponding to 271 and 188 CNV regions (CNVRs), which were obtained by merging overlapping CNVs. Out of these CNVRs, 99% were confirmed also by the CNVPartition program. These CNVRs covered 40.26 and 30.60 Mb of the chicken genome in the lean and fat lines, respectively. Moreover, CNVRs included 176 loss, 68 gain and 27 both (i.e. loss and gain within the same region) events in the lean line, and 143 loss, 25 gain and 20 both events in the fat line. Ten CNVRs were chosen for the validation experiment using qPCR method, and all of them were confirmed in at least one qPCR assay. We found a total of 886 genes located within these CNVRs, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed they could play various roles in a number of biological processes. Integrating the results of CNVRs, known quantitative trait loci (QTL) and selective sweeps for abdominal fat content suggested that some genes (including SLC9A3, GNAL, SPOCK3, ANXA10, HELIOS, MYLK, CCDC14, SPAG9, SOX5, VSNL1, SMC6, GEN1, MSGN1 and ZPAX) may be important for abdominal fat deposition in the chicken. Conclusions Our study provided a genome-wide CNVR map of the chicken genome, thereby contributing to our understanding of genomic structural variations and their potential roles in abdominal fat content in the chicken. [ABSTRACT FROM AUTHOR]
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- 2014
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29. Chromosomal imbalances in nasopharyngeal carcinoma: a meta-analysis of comparative genomic hybridization results.
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Xin Li, Ena Wang, Ying-dong Zhao, Jia-Qiang Ren, Ping Jin, Kai-Tai Yao, and Marincola, Francesco M.
- Subjects
NASOPHARYNX cancer ,CHROMOSOME abnormalities ,GENETICS ,EPSTEIN-Barr virus diseases ,META-analysis - Abstract
Nasopharyngeal carcinoma (NPC) is a highly prevalent disease in Southeast Asia and its prevalence is clearly affected by genetic background. Various theories have been suggested for its high incidence in this geographical region but to these days no conclusive explanation has been identified. Chromosomal imbalances identifiable through comparative genomic hybridization may shed some light on common genetic alterations that may be of relevance to the onset and progression of NPC. Review of the literature, however, reveals contradictory results among reported findings possibly related to factors associated with patient selection, stage of disease, differences in methodological details etc. To increase the power of the analysis and attempt to identify commonalities among the reported findings, we performed a meta-analysis of results described in NPC tissues based on chromosomal comparative genomic hybridization (CGH). This meta-analysis revealed consistent patters in chromosomal abnormalities that appeared to cluster in specific "hot spots" along the genome following a stage-dependent progression. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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30. Association between family members of dialysis patients and chronic kidney disease: a multicenter study in China.
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Kong, Xianglei, Liu, Li, Zuo, Li, Yuan, Ping, Li, Zhongxin, Li, Wenge, Cai, Meishun, Chen, Xiangmei, Jiang, Aili, Long, Gang, Xu, Jinsheng, Lin, Hongli, Wang, Shixiang, Huang, Wen, Wang, Yiping, Guo, Yidan, Cao, Po, Wu, Hua, Jia, Qiang, and Zhang, Luxia
- Abstract
Background: Family members of patients with end stage renal disease were reported to have an increased prevalence of chronic kidney disease (CKD). However, studies differentiated genetic and non-genetic family members are limited. We sought to investigate the prevalence of CKD among fist-degree relatives and spouses of dialysis patients in China.Methods: Seventeen dialysis facilities from 4 cities of China including 1062 first-degree relatives and 450 spouses of dialysis patients were enrolled. Sex- and age- matched controls were randomly selected from a representative sample of general population in Beijing. CKD was defined as decreased estimated glomerular (eGFR<60 mL/min/1.73 m2) or albuminuria.Results: The prevalence of eGFR less than 60 mL/min/1.73 m2, albuminuria and the overall prevalence of CKD in dialysis spouses were compared with their counterpart controls, which was 3.8% vs. 7.8% (P<0.01), 16.8% vs. 14.6% (P=0.29) and 18.4% vs. 19.8% (P=0.61), respectively. The prevalence of eGFR less than 60 mL/min/1.73 m2, albuminuria and the overall prevalence of CKD in dialysis relatives were also compared with their counterpart controls, which was 1.5% vs. 2.4% (P=0.12), 14.4% vs. 8.4% (P<0.01) and 14.6% vs. 10.5% (P<0.01), respectively. Multivariable Logistic regression analysis indicated that being spouses of dialysis patients is negatively associated with presence of low eGFR, and being relatives of dialysis patients is positively associated with presence of albuminuria.Conclusions: The association between being family members of dialysis patients and presence of CKD is different between first-degree relatives and spouses. The underlying mechanisms deserve further investigation. [ABSTRACT FROM AUTHOR]- Published
- 2013
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31. Identification of genome-wide SNP-SNP interactions associated with important traits in chicken.
- Author
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Zhang H, Yu JQ, Yang LL, Kramer LM, Zhang XY, Na W, Reecy JM, and Li H
- Subjects
- Animals, Chickens growth & development, Epistasis, Genetic, Genome, Male, Phenotype, Testis growth & development, Chickens genetics, Polymorphism, Single Nucleotide
- Abstract
Background: In addition to additive genetic effects, epistatic interactions can play key roles in the control of phenotypic variation of traits of interest. In the current study, 475 male birds from lean and fat chicken lines were utilized as a resource population to detect significant epistatic effects associated with growth and carcass traits., Results: A total of 421 significant epistatic effects were associated with testis weight (TeW), from which 11 sub-networks (Sub-network1 to Sub-network11) were constructed. In Sub-network1, which was the biggest network, there was an interaction between GGA21 and GGAZ. Three genes on GGA21 (SDHB, PARK7 and VAMP3) and nine genes (AGTPBP1, CAMK4, CDC14B, FANCC, FBP1, GNAQ, PTCH1, ROR2 and STARD4) on GGAZ that might be potentially important candidate genes for testis growth and development were detected based on the annotated gene function. In Sub-network2, there was a SNP on GGA19 that interacted with 8 SNPs located on GGA10. The SNP (Gga_rs15834332) on GGA19 was located between C-C motif chemokine ligand 5 (CCL5) and MIR142. There were 32 Refgenes on GGA10, including TCF12 which is predicted to be a target gene of miR-142-5p. We hypothesize that miR-142-5p and TCF12 may interact with one another to regulate testis growth and development. Two genes (CDH12 and WNT8A) in the same cadherin signaling pathway were implicated as potentially important genes in the control of metatarsus circumference (MeC). There were no significant epistatic effects identified for the other carcass and growth traits, e.g. heart weight (HW), liver weight (LW), spleen weight (SW), muscular and glandular stomach weight (MGSW), carcass weight (CW), body weight (BW1, BW3, BW5, BW7), chest width (ChWi), metatarsus length (MeL)., Conclusions: The results of the current study are helpful to better understand the genetic basis of carcass and growth traits, especially for testis growth and development in broilers.
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- 2017
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32. A novel swine model for evaluation of dyslipidemia and atherosclerosis induced by human CETP overexpression.
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Chen T, Sun M, Wang JQ, Cui JJ, Liu ZH, and Yu B
- Subjects
- Animals, Animals, Genetically Modified, Atherosclerosis etiology, Cholesterol blood, Cholesterol genetics, Cholesterol Ester Transfer Proteins metabolism, Disease Models, Animal, Dyslipidemias etiology, Female, Gene Expression, Humans, Lipoproteins, LDL genetics, Lipoproteins, LDL metabolism, Atherosclerosis genetics, Cholesterol Ester Transfer Proteins genetics, Dyslipidemias genetics, Sus scrofa genetics
- Abstract
Background: The mechanism of cholesteryl ester transfer protein (CETP) in lipid metabolism is still unclear. Furthermore, the relationship of CETP and atherosclerosis (AS) has been controversial. As pigs are a good model for both lipid and AS research, we investigated the lipid metabolism of human CETP (hCETP) transgenic pigs and explored the mechanism of CETP in lipid modulation., Methods: Plasmids expressing the hCETP gene were designed, successfully constructed, and transfected into porcine fetal fibroblasts by liposomes. Using somatic cell nuclear transfer technology and embryonic transfer, hCETP transgenic pigs were generated. After the DNA, RNA, and protein levels were identified, positive hCETP transgenic pigs were selected. Blood samples were collected at different ages to evaluate the phenotypes of biochemical markers, and the metabolomes of plasma samples were analyzed by liquid mass spectrometry., Results: Eight positive hCETP transgenic pigs and five negative cloned pigs were generated by transgenic technology. Finally, five hCETP transgenic and five cloned pigs were grown healthily. After feeding with a normal diet, hCETP transgenic pigs compared with unmodified pigs had no significant differences in body weight, liver function, kidney function, or plasma ions, while total cholesterol and low-density lipoprotein were higher than in unmodified pigs, and high-density lipoprotein was significantly decreased. Metabolomics analysis showed that there were differences in metabolic components between hCETP transgenic pigs, cloned pigs, and unmodified pigs., Conclusions: In this study, we created hCETP transgenic pigs that could serve as an excellent model for lipid disorders and atherosclerosis.
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- 2017
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33. Serum selenium level and gestational diabetes mellitus: a systematic review and meta-analysis.
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Kong FJ, Ma LL, Chen SP, Li G, and Zhou JQ
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- Adult, Diabetes, Gestational epidemiology, Female, Humans, Pregnancy, Pregnancy Trimester, Third, Racial Groups, Diabetes, Gestational blood, Selenium blood
- Abstract
Background: The association between serum selenium level and gestational diabetes mellitus (GDM) is controversial. The aim of our study was to systematically review available literature linking selenium to GDM for a comprehensive understanding of the relationship between serum selenium level and GDM in human., Methods: PubMed, The Cochrane Library and Medline were searched for studies published up to August 2016. Manual searches of references of the relevant original studies were carried out. Pooled estimates were measured using the fixed or random effect model. Overall effect was reported in a standard mean difference (SMD). All data were analyzed with Review Manager 5.3 and Stata 12.0., Results: Of 44 references reviewed, seven studies involving 569 patients met our inclusion criteria and contributed to meta-analysis. All the studies were used to evaluate the relationship between serum selenium level and GDM. Selenium level was significantly lower in women with GDM than those without GDM (SMD = -1.17; 95 % CI: -1.98 to -0.35, P = 0.005). Subgroup analysis showed that such trend was consistent within the non-Caucasian population (Asia: SMD = -2.82; 95 % CI: -5.21 to -0.43, P = 0.02; Africa: SMD = -0.56; 95 % CI: -1.07 to -0.05, P = 0.03) and in the third trimester (SMD = -1.78; 95 % CI: -3.04 to -0.52, P = 0.006), but not within the Caucasian population (Europe: SMD = -0.6; 95 % CI: -1.98 to 0.78, P = 0.39) or in the second trimester (SMD = -0.68; 95 % CI: -1.6 to 0.25, P = 0.15)., Conclusions: The available evidences suggested that serum selenium level was lower in women with GDM than those with normal glucose tolerance, especially within the non-Caucasian population and in the third trimester. However, well-designed prospective studies are needed to understand dynamic associations between selenium status and GDM risk.
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- 2016
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34. Lessons on malaria control in the ethnic minority regions in Northern Myanmar along the China border, 2007-2014.
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Wang RB, Dong JQ, Xia ZG, Cai T, Zhang QF, Zhang Y, Tian YH, Sun XY, Zhang GY, Li QP, Xu XY, Li JY, and Zhang J
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Cross-Sectional Studies, Ethnicity, Female, Health Education, Humans, Incidence, Malaria epidemiology, Malaria transmission, Male, Middle Aged, Myanmar epidemiology, Prevalence, Young Adult, Malaria prevention & control
- Abstract
Background: For many countries where malaria is endemic, the burden of malaria is high in border regions. In ethnic minority areas along the Myanmar-China border, residents have poor access to medical care for diagnosis and treatment, and there have been many malaria outbreaks in such areas. Since 2007, with the support of the Global Fund to Fight AIDS, Tuberculosis and Malaria (GFATM), a malaria control project was introduced to reduce the malaria burden in several ethnic minority regions., Methods: A malaria control network was established during the period from 2007 to 2014. Multiple malaria interventions, including diagnosis, treatment, distribution of LLINs and health education, were conducted to improve the accessibility and quality of malaria control services for local residents. Annual cross-sectional surveys were conducted to evaluate intervention coverage and indicators of malaria transmission., Results: In ethnic minority regions where a malaria control network was established, both the annual malaria incidence (19.1 per thousand per year, in 2009; 8.7, in 2014) and malaria prevalence (13.6 % in 2008; 0.43 % in 2014) decreased dramatically during the past 5-6 years. A total of 851 393 febrile patients were detected, 202 598 malaria cases (including confirmed cases and suspected cases) were treated, and 759 574 LLINs were delivered to populations at risk. Of households in 2012, 73.9 % had at least one ITNs/LLINs (vs. 28.3 %, in 2008), and 50.7 % of children less than 5 years and 50.3 % of pregnant women slept under LLINs the night prior to their visit. Additionally, malaria knowledge was improved in 68.4 % of residents., Conclusion: There has been great success in improving malaria control in these regions from 2007 to 2014. Malaria burdens have decreased, especially in KOK and WA. The continued maintenance of sustainable malaria control networks in these regions may be a long-term process, due to regional conflicts and the lack of funds, technology, and health workers. Furthermore, information and scientific support from the international community should be offered to these ethnic minority regions to uphold recent achievements.
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- 2016
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35. Effectiveness and impact of the cross-border healthcare model as implemented by non-governmental organizations: case study of the malaria control programs by health poverty action on the China-Myanmar border.
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Zhang J, Dong JQ, Li JY, Zhang Y, Tian YH, Sun XY, Zhang GY, Li QP, Xu XY, and Cai T
- Subjects
- China epidemiology, Emigration and Immigration, Humans, Malaria epidemiology, Myanmar epidemiology, Prevalence, Communicable Disease Control organization & administration, Malaria prevention & control, Organizations, Poverty
- Abstract
Background: In the Yunnan province of China, 18 counties in six prefectures border Myanmar. Due to its particular combination of geographic features, climate conditions, and cultural landscape, the area provides a suitable environment for the spread of insect-borne diseases such as malaria. In five identified Myanmar Special Regions along the China-Myanmar border, economic development is lagging, people live in extreme poverty, and the healthcare system is fragile. Coupled with political and other reasons, this precludes malaria control work to be effectively carried out in Myanmar, resulting in a heavy burden of the disease. Frequent population movements and favorable conditions for malaria transmission on the border fuel difficulties in controlling and eliminating the spread of the disease in the area., Case Presentation: To reduce the prevalence of malaria in the China-Myanmar border area and improve healthcare services for local residents in this particular environment, Health Poverty Action (HPA) has provided malaria aid in the area since the beginning of 2006, as a sub-recipient of the China Global Fund Malaria Programs. In this case study, we examined HPA's activities as part of its malaria control programs in the area, analyzed and summarized the effectiveness and impact of the cross-border healthcare model as implemented by non-governmental organizations, and put forward suggestions for cross-border health aid models and for the prevention of malaria transmission in the Greater Mekong Subregion., Conclusions: HPA had carried out a great quantity of successful malaria control activities in border areas between China and Myanmar, strengthened the partnership and established the collaboration, coordination and cooperation channels among stakeholders. HPA has laid good groundwork and developed its valuable model that could be highlighted and referenced.
- Published
- 2016
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36. Detection of genome-wide copy number variations in two chicken lines divergently selected for abdominal fat content.
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Zhang H, Du ZQ, Dong JQ, Wang HX, Shi HY, Wang N, Wang SZ, and Li H
- Subjects
- Adiposity genetics, Animals, Databases, Nucleic Acid, Male, Quantitative Trait Loci, Quantitative Trait, Heritable, Reproducibility of Results, Abdominal Fat metabolism, Chickens genetics, DNA Copy Number Variations, Genome-Wide Association Study
- Abstract
Background: The chicken (Gallus gallus) is an important model organism that bridges the evolutionary gap between mammals and other vertebrates. Copy number variations (CNVs) are a form of genomic structural variation widely distributed in the genome. CNV analysis has recently gained greater attention and momentum, as the identification of CNVs can contribute to a better understanding of traits important to both humans and other animals. To detect chicken CNVs, we genotyped 475 animals derived from two broiler chicken lines divergently selected for abdominal fat content using chicken 60 K SNP array, which is a high-throughput method widely used in chicken genomics studies., Results: Using PennCNV algorithm, we detected 438 and 291 CNVs in the lean and fat lines, respectively, corresponding to 271 and 188 CNV regions (CNVRs), which were obtained by merging overlapping CNVs. Out of these CNVRs, 99% were confirmed also by the CNVPartition program. These CNVRs covered 40.26 and 30.60 Mb of the chicken genome in the lean and fat lines, respectively. Moreover, CNVRs included 176 loss, 68 gain and 27 both (i.e. loss and gain within the same region) events in the lean line, and 143 loss, 25 gain and 20 both events in the fat line. Ten CNVRs were chosen for the validation experiment using qPCR method, and all of them were confirmed in at least one qPCR assay. We found a total of 886 genes located within these CNVRs, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed they could play various roles in a number of biological processes. Integrating the results of CNVRs, known quantitative trait loci (QTL) and selective sweeps for abdominal fat content suggested that some genes (including SLC9A3, GNAL, SPOCK3, ANXA10, HELIOS, MYLK, CCDC14, SPAG9, SOX5, VSNL1, SMC6, GEN1, MSGN1 and ZPAX) may be important for abdominal fat deposition in the chicken., Conclusions: Our study provided a genome-wide CNVR map of the chicken genome, thereby contributing to our understanding of genomic structural variations and their potential roles in abdominal fat content in the chicken.
- Published
- 2014
- Full Text
- View/download PDF
37. Mineral and bone disorder in Chinese dialysis patients: a multicenter study.
- Author
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Kong X, Zhang L, Zhang L, Chen N, Gu Y, Yu X, Liu W, Chen J, Peng L, Yuan W, Wu H, Chen W, Fan M, He L, Ding F, Chen X, Xiong Z, Zhang J, Jia Q, Shi W, Xing C, Tang X, Hou F, Shu G, Mei C, Wang L, Xu D, Ni Z, Zuo L, Wang M, and Wang H
- Subjects
- Adult, Aged, Biomarkers blood, Bone Diseases diagnosis, Calcium blood, Cross-Sectional Studies, Female, Humans, Hyperphosphatemia diagnosis, Male, Middle Aged, Parathyroid Hormone blood, Phosphorus blood, Asian People ethnology, Bone Diseases blood, Bone Diseases ethnology, Hyperphosphatemia blood, Hyperphosphatemia ethnology, Renal Dialysis adverse effects
- Abstract
Background: Mineral and bone disorder (MBD) in patients with chronic kidney disease is associated with increased morbidity and mortality. Studies regarding the status of MBD treatment in developing countries, especially in Chinese dialysis patients are extremely limited., Methods: A cross-sectional study of 1711 haemodialysis (HD) patients and 363 peritoneal dialysis (PD) patients were enrolled. Parameters related to MBD, including serum phosphorus (P), calcium (Ca), intact parathyroid hormone (iPTH) were analyzed. The achievement of MBD targets was compared with the results from the Dialysis Outcomes and Practice Study (DOPPS) 3 and DOPPS 4. Factors associated with hyperphosphatemia were examined., Results: Total 2074 dialysis patients from 28 hospitals were involved in this study. Only 38.5%, 39.6% and 26.6% of them met the Kidney Disease Outcomes Quality Initiative (K/DOQI) defined targets for serum P, Ca and iPTH levels. Serum P and Ca levels were statistically higher (P < 0.05) in the HD patients compared with those of PD patients, which was (6.3 ± 2.1) mg/dL vs (5.7 ± 2.0) mg/dL and (9.3 ± 1.1) mg/dL vs (9.2 ± 1.1) mg/dL, respectively. Serum iPTH level were statistically higher in the PD patients compared with those of HD patients (P = 0.03). The percentage of patients reached the K/DOQI targets for P (37.6% vs 49.8% vs 54.5%, P < 0.01), Ca (38.6% vs 50.4% vs 56.0%, P < 0.01) and iPTH (26.5% vs 31.4% vs 32.1%, P < 0.01) were lower among HD patients, compared with the data from DOPPS 3 and DOPPS 4. The percentage of patients with serum phosphorus level above 5.5 mg/dL was 57.4% in HD patients and 47.4% in PD patients. Age, dialysis patterns and region of residency were independently associated with hyperphosphatemia., Conclusions: Status of MBD is sub-optimal among Chinese patients receiving dialysis. The issue of hyperphosphatemia is prominent and needs further attention.
- Published
- 2012
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38. Characterization and specificity of the linear epitope of the enterovirus 71 VP2 protein.
- Author
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Kiener TK, Jia Q, Lim XF, He F, Meng T, Chow VT, and Kwang J
- Subjects
- Amino Acid Substitution, Animals, Antibodies, Monoclonal biosynthesis, Antibodies, Monoclonal immunology, Antibodies, Viral biosynthesis, Antibodies, Viral immunology, Antibody Specificity immunology, Antigens, Viral genetics, Capsid Proteins genetics, Cross Reactions immunology, Enterovirus Infections diagnosis, Epitope Mapping, Epitopes genetics, Humans, Mice, Mutation, Antigens, Viral immunology, Capsid Proteins chemistry, Capsid Proteins immunology, Enterovirus A, Human chemistry, Enterovirus A, Human immunology, Epitopes immunology
- Abstract
Background: Enterovirus 71 (EV71) has emerged as a major causative agent of hand, foot and mouth disease in the Asia-Pacific region over the last decade. Hand, foot and mouth disease can be caused by different etiological agents from the enterovirus family, mainly EV71 and coxsackieviruses, which are genetically closely related. Nevertheless, infection with EV71 may occasionally lead to high fever, neurologic complications and the emergence of a rapidly fatal syndrome of pulmonary edema associated with brainstem encephalitis. The rapid progression and high mortality of severe EV71 infection has highlighted the need for EV71-specific diagnostic and therapeutic tools. Monoclonal antibodies are urgently needed to specifically detect EV71 antigens from patient specimens early in the infection process. Furthermore, the elucidation of viral epitopes will contribute to the development of targeted therapeutics and vaccines., Results: We have identified the monoclonal antibody 7C7 from a screen of hybridoma cells derived from mice immunized with the EV71-B5 strain. The linear epitope of 7C7 was mapped to amino acids 142-146 (EDSHP) of the VP2 capsid protein and was characterized in detail. Mutational analysis of the epitope showed that the aspartic acid to asparagine mutation of the EV71 subgenogroup A (BrCr strain) did not interfere with antibody recognition. In contrast, the serine to threonine mutation at position 144 of VP2, present in recently emerged EV71-C4 China strains, abolished antigenicity. Mice injected with this virus strain did not produce any antibodies against the VP2 protein. Immunofluorescence and Western blotting confirmed that 7C7 specifically recognized EV71 subgenogroups and did not cross-react to Coxsackieviruses 4, 6, 10, and 16. 7C7 was successfully used as a detection antibody in an antigen-capture ELISA assay., Conclusions: Detailed mapping showed that the VP2 protein of Enterovirus 71 contains a single, linear, non-neutralizing epitope, spanning amino acids 142-146 which are located in the VP2 protein's E-F loop. The S/T(144) mutation in this epitope confers a loss of VP2 antigenicity to some newly emerged EV71-C4 strains from China. The corresponding monoclonal antibody 7C7 was used successfully in an AC-ELISA and did not cross-react to coxsackieviruses 4, 6, 10, and 16 in immunofluorescence assay and Western blots. 7C7 is the first monoclonal antibody described, that can differentiate Coxsackievirus 16 from Enterovirus 71.
- Published
- 2012
- Full Text
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39. Chromosomal imbalances in nasopharyngeal carcinoma: a meta-analysis of comparative genomic hybridization results.
- Author
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Li X, Wang E, Zhao YD, Ren JQ, Jin P, Yao KT, and Marincola FM
- Abstract
Nasopharyngeal carcinoma (NPC) is a highly prevalent disease in Southeast Asia and its prevalence is clearly affected by genetic background. Various theories have been suggested for its high incidence in this geographical region but to these days no conclusive explanation has been identified. Chromosomal imbalances identifiable through comparative genomic hybridization may shed some light on common genetic alterations that may be of relevance to the onset and progression of NPC. Review of the literature, however, reveals contradictory results among reported findings possibly related to factors associated with patient selection, stage of disease, differences in methodological details etc. To increase the power of the analysis and attempt to identify commonalities among the reported findings, we performed a meta-analysis of results described in NPC tissues based on chromosomal comparative genomic hybridization (CGH). This meta-analysis revealed consistent patters in chromosomal abnormalities that appeared to cluster in specific "hot spots" along the genome following a stage-dependent progression.
- Published
- 2006
- Full Text
- View/download PDF
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