1. Anti-proliferative and anti-migration effects of Polish propolis combined with Hypericum perforatum L. on glioblastoma multiforme cell line U87MG
- Author
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Renata Markiewicz-Żukowska, Anna Puścion-Jakubik, Sylwia K. Naliwajko, Maria H. Borawska, Justyna Moskwa, and Jolanta Soroczyńska
- Subjects
0301 basic medicine ,Antineoplastic Agents ,Pharmacology ,Glioblastoma multiforme ,Propolis ,H. perforatum L ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Glioma ,Cell Line, Tumor ,Medicine ,Humans ,Secretion ,Cell Proliferation ,DNA synthesis ,business.industry ,Plant Extracts ,Hypericum perforatum ,Cell migration ,General Medicine ,medicine.disease ,030104 developmental biology ,Complementary and alternative medicine ,Apoptosis ,Cell culture ,030220 oncology & carcinogenesis ,business ,Glioblastoma ,Hypericum ,Research Article - Abstract
Background Propolis and Hypericum perforatum L. are natural products which contain many active compounds and have numerous beneficial effects, including an antitumor effect. Gliobmastoma multiforme (GBM) is a common primary brain tumor with poor prognosis and limited treatment options. In this study, the effect of propolis (EEP) combined with H. perforatum L. (HPE) on glioblastoma cell line U87MG was investigated for the first time. Methods Anti-proliferative activity of EEP, HPE and their combination (EEP + HPE) was determined by a cytotoxicity test, DNA binding by [3H]-thymidine incorporation and cell migration assay. Anti-metastatic properties in U87MG treated with EEP, HPE and EEP + HPE were estimated on cells migration test (scratch assay) and metalloproteinases (MMP2 and MMP9) secretion (gelatin zymography). Results Combination of HPE and EEP extracts was found to have a time- and dose-dependent inhibitory effect on the viability of U87MG cells. This effect was significantly higher (p
- Published
- 2016