8 results on '"Li, Yue‐Zhong"'
Search Results
2. Engineering the acyltransferase domain of epothilone polyketide synthase to alter the substrate specificity
- Author
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Wang, Huimin, Liang, Junheng, Yue, Qianwen, Li, Long, Shi, Yan, Chen, Guosong, Li, Yue-zhong, Bian, Xiaoying, Zhang, Youming, Zhao, Guoping, and Ding, Xiaoming
- Published
- 2021
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3. CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus.
- Author
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Peng, Ran, Wang, Ye, Feng, Wan-wan, Yue, Xin-jing, Chen, Jiang-he, Hu, Xiao-zhuang, Li, Zhi-feng, Sheng, Duo-hong, Zhang, You-ming, and Li, Yue-zhong
- Subjects
CRISPRS ,MYXOCOCCUS xanthus ,EUKARYOTIC cells ,RNA polymerases ,MYXOBACTERALES ,SIGMA factor (Transcription factor) - Abstract
Background: The CRISPR/dCas9 system is a powerful tool to activate the transcription of target genes in eukaryotic or prokaryotic cells, but lacks assays in complex conditions, such as the biosynthesis of secondary metabolites. Results: In this study, to improve the transcription of the heterologously expressed biosynthetic genes for the production of epothilones, we established the CRISPR/dCas9-mediated activation technique in Myxococcus xanthus and analyzed some key factors involving in the CRISPR/dCas9 activation. We firstly optimized the cas9 codon to fit the M. xanthus cells, mutated the gene to inactivate the nuclease activity, and constructed the dCas9-activator system in an epothilone producer. We compared the improvement efficiency of different sgRNAs on the production of epothilones and the expression of the biosynthetic genes. We also compared the improvement effects of different activator proteins, the ω and α subunits of RNA polymerase, and the sigma factors σ54 and CarQ. By using a copper-inducible promoter, we determined that higher expressions of dCas9-activator improved the activation effects. Conclusions: Our results showed that the CRISPR/dCas-mediated transcription activation is a simple and broadly applicable technique to improve the transcriptional efficiency for the production of secondary metabolites in micro-organisms. This is the first time to construct the CRISPR/dCas9 activation system in myxobacteria and the first time to assay the CRISPR/dCas9 activations for the biosynthesis of microbial secondary metabolites. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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4. Increasing on-target cleavage efficiency for CRISPR/Cas9-induced large fragment deletion in Myxococcus xanthus.
- Author
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Yang, Ying-jie, Wang, Ye, Li, Zhi-feng, Gong, Ya, Zhang, Peng, Hu, Wen-chao, Sheng, Duo-hong, and Li, Yue-zhong
- Subjects
CRISPRS ,GENOME editing ,MYXOCOCCUS xanthus ,DNA ,TRANSFER RNA ,GRAM-negative bacteria - Abstract
Background: The CRISPR/Cas9 system is a powerful tool for genome editing, in which the sgRNA binds and guides the Cas9 protein for the sequence-specific cleavage. The protocol is employable in different organisms, but is often limited by cell damage due to the endonuclease activity of the introduced Cas9 and the potential off-target DNA cleavage from incorrect guide by the 20 nt spacer. Results: In this study, after resolving some critical limits, we have established an efficient CRISPR/Cas9 system for the deletion of large genome fragments related to the biosynthesis of secondary metabolites in Myxococcus xanthus cells. We revealed that the high expression of a codon-optimized cas9 gene in M. xanthus was cytotoxic, and developed a temporally high expression strategy to reduce the cell damage from high expressions of Cas9. We optimized the deletion protocol by using the tRNA-sgRNA-tRNA chimeric structure to ensure correct sgRNA sequence. We found that, in addition to the position-dependent nucleotide preference, the free energy of a 20 nt spacer was a key factor for the deletion efficiency. Conclusions: By using the developed protocol, we achieved the CRISPR/Cas9-induced deletion of large biosynthetic gene clusters for secondary metabolites in M. xanthus DK1622 and its epothilone-producing mutant. The findings and the proposals described in this paper were suggested to be workable in other organisms, for example, other Gram negative bacteria with high GC content. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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5. The complete genome sequence and analysis of a plasmid-bearing myxobacterial strain Myxococcus fulvus 124B02 (M 206081).
- Author
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Chen, Xiao-jing, Han, Kui, Feng, Jing, Zhuo, Li, Li, Ya-jie, and Li, Yue-zhong
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NUCLEOTIDE sequencing ,PLASMIDS ,MYXOBACTERALES ,MYXOCOCCUS ,PHYLOGENY ,PROTEOBACTERIA ,ANIMAL social behavior ,GENETIC engineering - Abstract
Myxobacteria, phylogenetically located in the delta division of the Proteobacteria, are well known for characterized social behaviors and large genomes of more than 9 Mb in size. Myxococcus fulvus is a typical species of the genus Myxococcus in the family Myxococcaceae. M. fulvus 124B02, originally isolated from a soil sample collected in Northeast China, is the one and only presently known myxobacterial strain that harbors an endogenous autonomously replicating plasmid, named pMF1. The endogenous plasmid is of importance for understanding the genome evolution of myxobacteria, as well as for the development of genetic engineering tools in myxobacteria. Here we describe the complete genome sequence of this organism. M. fulvus 124B02 consists of a circular chromosome with a total length of 11,048,835 bp and a circular plasmid of 18,634 bp. Comparative genomic analyses suggest that pMF1 has a longstanding sustention within myxobacteria, and probably contributes to the genome expansion of myxobacteria. [ABSTRACT FROM AUTHOR]
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- 2016
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6. A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis.
- Author
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Yue XJ, Cui XW, Zhang Z, Peng R, Zhang P, Li ZF, and Li YZ
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- Multigene Family, Promoter Regions, Genetic, Proteobacteria genetics, Proteobacteria metabolism, Secondary Metabolism, Suppression, Genetic, Epothilones biosynthesis, Gene Expression Regulation, Bacterial, Inverted Repeat Sequences, Myxococcales genetics, Myxococcales metabolism, Transcription, Genetic
- Abstract
Background: Microbial secondary metabolism is regulated by a complex and mostly-unknown network of global and pathway-specific regulators. A dozen biosynthetic gene clusters for secondary metabolites have been reported in myxobacteria, but a few regulation factors have been identified., Results: We identified a transcription regulator Esi for the biosynthesis of epothilones. Inactivation of esi promoted the epothilone production, while overexpression of the gene suppressed the production. The regulation was determined to be resulted from the transcriptional changes of epothilone genes. Esi was able to bind, probably via the N-terminus of the protein, to an inverted repeat sequence in the promoter of the epothilone biosynthetic gene cluster. The Esi-homologous sequences retrieved from the RefSeq database are all of the Proteobacteria. However, the Esi regulation is not universal in myxobacteria, because the esi gene exists only in a few myxobacterial genomes., Conclusions: Esi binds to the epothilone promoter and down-regulates the transcriptional level of the whole gene cluster to affect the biosynthesis of epothilone. This is the first transcription regulator identified for epothilone biosynthesis.
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- 2017
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7. Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes in Myxococcus xanthus.
- Author
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Zhu LP, Yue XJ, Han K, Li ZF, Zheng LS, Yi XN, Wang HL, Zhang YM, and Li YZ
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- Bacterial Proteins genetics, Bacterial Proteins metabolism, Epothilones genetics, Epothilones metabolism, Gene Expression, Mutagenesis, Insertional, Myxococcus xanthus metabolism, Myxococcus xanthus genetics, Transcriptome
- Abstract
Background: Exotic genes, especially clustered multiple-genes for a complex pathway, are normally integrated into chromosome for heterologous expression. The influences of insertion sites on heterologous expression and allotropic expressions of exotic genes on host remain mostly unclear., Results: We compared the integration and expression efficiencies of single and multiple exotic genes that were inserted into Myxococcus xanthus genome by transposition and attB-site-directed recombination. While the site-directed integration had a rather stable chloramphenicol acetyl transferase (CAT) activity, the transposition produced varied CAT enzyme activities. We attempted to integrate the 56-kb gene cluster for the biosynthesis of antitumor polyketides epothilones into M. xanthus genome by site-direction but failed, which was determined to be due to the insertion size limitation at the attB site. The transposition technique produced many recombinants with varied production capabilities of epothilones, which, however, were not paralleled to the transcriptional characteristics of the local sites where the genes were integrated. Comparative transcriptomics analysis demonstrated that the allopatric integrations caused selective changes of host transcriptomes, leading to varied expressions of epothilone genes in different mutants., Conclusions: With the increase of insertion fragment size, transposition is a more practicable integration method for the expression of exotic genes. Allopatric integrations selectively change host transcriptomes, which lead to varied expression efficiencies of exotic genes.
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- 2015
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8. Cultivation to improve in vivo solubility of overexpressed arginine deiminases in Escherichia coli and the enzyme characteristics.
- Author
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Wang Y and Li YZ
- Subjects
- Arginine metabolism, Bacterial Proteins chemistry, Bacterial Proteins genetics, Base Sequence, Citrulline metabolism, Hydrogen-Ion Concentration, Hydrolases chemistry, Hydrolases genetics, Listeria enzymology, Molecular Chaperones genetics, Molecular Chaperones metabolism, Molecular Sequence Data, Pseudomonas putida enzymology, Recombinant Proteins biosynthesis, Recombinant Proteins chemistry, Recombinant Proteins genetics, Solubility, Bacterial Proteins metabolism, Escherichia coli metabolism, Hydrolases metabolism
- Abstract
Background: Overexpression of foreign genes in Escherichia coli cells is an efficient means to obtain recombinant proteins. The technique is, however, often hampered by misfolding, degradation, aggregation and formation in inclusion bodies of products., Results: In this study, we reported that in vivo solubility of overexpressed arginine deiminases (ADI) improved by changing the cultivation conditions. ADI is enzymes that convert L-arginine to L-citrulline. After codon optimization, we synthesized the ADI gene of Pseudomonas putida and constructed it for overexpression in E. coli cells. The rADI products were mainly in inclusion body forms. We performed a series of optimization to enhance solubility of the protein. Co-expression with the GroES-GroEL chaperone team increased approximately 5-fold of the rADI activity. In addition the combination of L-arginine and D-glucose in the Luria-Bertani (LB) growth medium further increased the total activity to about 15 times. Separate L-arginine and D-glucose or the addition of other saccharides or amino acids had no such effects. The solubilization effects of the combination of L-arginine and D-glucose were further confirmed in the overexpression of another ADI from Listeria welshimeri. The enzymatic and conversion characteristics of the rADI products were further determined., Conclusions: Combined addition of L-arginine and D-glucose in the LB medium significantly improved in vivo solubility of rADI proteins. The present study suggested a new strategy to increase the solubilization of overexpressed recombinant proteins in E. coli cells.
- Published
- 2014
- Full Text
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