10 results on '"Linlin Wang"'
Search Results
2. Associations between prenatal exposure to cadmium and lead with neural tube defect risks are modified by single-nucleotide polymorphisms of fetal MTHFR and SOD2: a case–control study
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Mengyuan Liu, Linlin Wang, Zaiming Su, Aiguo Ren, Jinhui Yu, Zhiwen Li, Ying Sun, Jie Zhang, Yaqiong Liu, Lei Jin, and Qing Xie
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congenital, hereditary, and neonatal diseases and abnormalities ,Health, Toxicology and Mutagenesis ,010501 environmental sciences ,Lower risk ,01 natural sciences ,Single-nucleotide polymorphisms ,Andrology ,03 medical and health sciences ,medicine ,Risk factor ,Superoxide dismutase 2 (SOD2) ,Neural tube defects ,030304 developmental biology ,0105 earth and related environmental sciences ,0303 health sciences ,Fetus ,biology ,Neural tube defect ,5,10-methylenetetrahydrofolatereductase (MTHFR) ,business.industry ,Research ,Public Health, Environmental and Occupational Health ,Case-control study ,Absolute risk reduction ,Odds ratio ,medicine.disease ,Industrial medicine. Industrial hygiene ,RC963-969 ,Lead ,Methylenetetrahydrofolate reductase ,biology.protein ,Public aspects of medicine ,RA1-1270 ,business ,Cadmium - Abstract
Background Prenatal exposure to heavy metals is implicated in the etiology of birth defects. We investigated whether concentrations of cadmium (Cd) and lead (Pb) in umbilical cord tissue are associated with risk for neural tube defects (NTDs) and whether selected genetic variants of the fetus modify their associations. Methods This study included 166 cases of NTD fetuses/newborns and 166 newborns without congenital malformations. Umbilical cord tissue was collected at birth or elective pregnancy termination. Cd and Pb concentrations were assessed by inductively coupled plasma-mass spectrometry, and 20 single-nucleotide polymorphisms (SNPs) in 9 genes were genotyped. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to estimate the risk for NTDs in association with metal concentrations or genotype using logistic regression. Multiplicative-scale interactions between the metals and genotypes on NTD risk were assessed with logistic regression, and additive-scale interactions were estimated with a non-linear mixed effects model. Results Higher concentrations of Cd were observed in the NTD group than in the control group, but no difference was found for Pb. Concentrations of Cd above the median level showed a risk effect, while the association between Pb and NTD risk was not significant in univariate analyses. The association of Cd was attenuated after adjusting for periconceptional folic acid supplementation. Fetuses with the AG and GG genotypes of rs4880 in SOD2 (superoxide dismutase 2) tended to have a lower risk, but fetuses with the CT and TT genotypes of rs1801133 in MTHFR (5,10-methylenetetrahydrofolatereductase) have a higher risk for NTDs when compared to their respective wild-type. rs4880 and Cd exhibited a multiplicative-scale interaction on NTD risk: the association between higher Cd and the risk for NTDs was increased by over fourfold in fetuses carrying the G allele [OR 4.43 (1.30–15.07)] compared to fetuses with the wild-type genotype. rs1801133 and Cd exposure showed an additive interaction, with a significant relative excess risk of interaction [RERI 0.64 (0.02–1.25)]. Conclusions Prenatal exposure to Cd may be a risk factor for NTDs, and the risk effect may be enhanced in fetuses who carry the G allele of rs4880 in SOD2 and T allele of rs1801133 in MTHFR.
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- 2021
3. Improved clinical outcomes of preimplantation genetic testing for aneuploidy using MALBAC-NGS compared with MDA-SNP array
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Fang Wang, Wenyan Song, Wenbin Niu, Jiawei Xu, Ying Li, Gang Li, Linlin Wang, Hao Shi, Haixia Jin, and Yingpu Sun
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Adult ,Male ,medicine.medical_specialty ,China ,Pregnancy Rate ,Aneuploidy ,Single-nucleotide polymorphism ,Fertilization in Vitro ,Single Center ,lcsh:Gynecology and obstetrics ,Polymorphism, Single Nucleotide ,Miscarriage ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Next generation sequencing ,Medicine ,SNP ,Humans ,Genetic Testing ,lcsh:RG1-991 ,Preimplantation Diagnosis ,030304 developmental biology ,Genetic testing ,Retrospective Studies ,0303 health sciences ,030219 obstetrics & reproductive medicine ,medicine.diagnostic_test ,business.industry ,Obstetrics ,Preimplantation genetic testing for aneuploidy ,MALBAC ,Pregnancy Outcome ,Obstetrics and Gynecology ,High-Throughput Nucleotide Sequencing ,medicine.disease ,Embryo Transfer ,Single nucleotide polymorphism ,Abortion, Spontaneous ,Blastocyst ,Karyotyping ,Female ,business ,SNP array ,Research Article - Abstract
Background To assess whether preimplantation genetic testing for aneuploidy with next generation sequencing (NGS) outweighs single nucleotide polymorphism (SNP) array in improving clinical outcomes. Methods A retrospective analysis of the clinical outcomes of patients who underwent PGT-A treatment in a single center from January 2013 to December 2017.A total of 1418 couples who underwent PGT-A treatment were enrolled, of which 805 couples used NGS for PGT-A, while the remaining 613 couples used SNP array for PGT-A. Clinical pregnancy rate, miscarriage rate and healthy baby rate were compared between the MALBAC-NGS-PGT-A and MDA-SNP-PGT-A groups. Results After testing karyotypes of 5771 biopsied blastocysts, 32.2% (1861/5771) were identified as chromosomally normal, while 67.8% were chromosomally abnormal. In terms of clinical outcomes, women in the MALBAC-NGS-PGT-A group had a significantly higher clinical pregnancy rate (50.5% vs 41.7%, p = 0.002) and healthy baby rate (39.6% vs 31.4%, p = 0.003), and a lower miscarriage rate (15.5% vs 22.8%, p = 0.036). Conclusion This is the largest study reporting the extensive application of NGS-based PGT-A, whilst comparing the clinical outcomes of MALBAC-NGS-PGT-A and MDA-SNP-PGT-A. The results provide greater evidence supporting the wider use of NGS in PGT-A, not only for its lower cost but also for its improved clinical outcomes compared to SNP-based PGT-A.
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- 2020
4. Sonographic features of the testicular adrenal rests tumors in patients with congenital adrenal hyperplasia: a single-center experience and literature review
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Li Ma, Linlin Wang, Xuepei Huang, Ruifeng Liu, Yu Xia, Yuxin Jiang, Li Zhang, Tiantian Ye, Jianchu Li, and Qingli Zhu
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,lcsh:Medicine ,030209 endocrinology & metabolism ,Malignancy ,Single Center ,030218 nuclear medicine & medical imaging ,Benign tumor ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Vascularity ,Testicular Neoplasms ,Ultrasound ,Biomarkers, Tumor ,Medicine ,Adrenal Rest Tumor ,Humans ,Pharmacology (medical) ,Congenital adrenal hyperplasia ,Child ,Genetics (clinical) ,Retrospective Studies ,Ultrasonography ,Adrenal Hyperplasia, Congenital ,business.industry ,Research ,Testicular adrenal rests tumor ,lcsh:R ,Echogenicity ,Mediastinum ,General Medicine ,medicine.disease ,medicine.anatomical_structure ,Child, Preschool ,Radiology ,medicine.symptom ,business - Abstract
Purpose Testicular adrenal rests tumor (TART) is a rare kind of benign tumor in the testis. It usually occurred secondary to congenital adrenal hyperplasia (CAH), a hormonal disorder caused by hydroxylase deficiency. As the first-line examination method, ultrasound provides crucial diagnostic information for TART, although misdiagnosis to malignancy is quite common because of its rare prevalence. We aimed to summarize the sonographic manifestations of TART to improve the diagnostic accuracy and specificity. Methods Eight CAH patients with TART identified by ultrasound in our medical center were retrospectively reviewed. Clinical and hormonal profile, semen analysis and treatment choices were collected. Sonographic examinations were performed at the first evaluation and interpreted by experienced radiologists individually. All patients received regular follow-up, and 5 patients undertook repeated scrotal ultrasound. A literature review of TART in CAH patients was conducted, with 123 patients from 23 articles since 1990 included. Results A total of 8 patients aged between 4 to 27 years old were enrolled. 7 of 8 (87.5%) patients exhibited bilateral testicular lesions. The sizes of the testicular lesions were between 0.18 ml to 5.68 ml, and all showed a clear boundary. 10/15 (66.7%) lesions were homogenously hypoechoic, 4/15 (26.7%) were heterogeneously iso-hypoechoic, and 1/15 (6.7%) were homogenously isoechoic. 10/15 (66.7%) lesions were hyper-vascular. The longitudinal follow-up of 5 patients showed testicular lesions changed in terms of size, echogenicity, and vascularity after steroid treatment. A potential correlation may exist between ACTH levels and tumor size (p = 0.066). From the literature review, 100/123 (81%) patients got bilateral lesions, and 95% of them were located near the mediastinum. 80/103 (78%) lesions exhibited a clear boundary, and predominant lesions (74%) were hypoechogenic. Vascularity was with great diversity. Seventy-nine lesions of 44 patients were followed-up by scrotal ultrasound, among which 29 (37%) remained unchanged, 29(37%) shrank, and 21(27%) disappeared. Conclusions Key sonographic characteristics of TART are: resembled lesions on both testes, located near the mediastinum, clear boundary, and changed in size or echogenicity after steroid treatment. These features can help radiologists to make an accurate diagnosis of TART.
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- 2019
5. Modulating tumor infiltrating myeloid cells to enhance bispecific antibody-driven T cell infiltration and anti-tumor response.
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Park, Jeong A., Linlin Wang, and Cheung, Nai‑Kong V.
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Background: Tumor microenvironment (TME) is a dynamic cellular milieu to promote tumor angiogenesis, growth, proliferation, and metastasis, while derailing the host anti-tumor response. TME impedes bispecific antibody (BsAb) or chimeric antigen receptor (CAR)-driven T cells infiltration, survival, and cytotoxic efficacy. Modulating tumor infiltrating myeloid cells (TIMs) could potentially improve the efficacy of BsAb. Methods: We evaluated the effects of TIM modulation on BsAb-driven T cell infiltration into tumors, their persistence, and in vivo anti-tumor response. Anti-GD2 BsAb and anti-HER2 BsAb built on IgG-[L]-scFv platform were tested against human cancer xenografts in BALB-Rag2-/-IL-2R-γc-KO (BRG) mice. Depleting antibodies specific for polymorphonuclear myeloid-derived suppressor cell (PMN-MDSC), monocytic MDSC (M-MDSC), and tumor associated macrophage (TAM) were used to study the role of each TIM component. Dexamethasone, an established anti-inflammatory agent, was tested for its effect on TIMs. Results: BsAb-driven T cells recruited myeloid cells into human tumor xenografts. Each TIM targeting therapy depleted cells of interest in blood and in tumors. Depletion of PMN-MDSCs, M-MDSCs, and particularly TAMs was associated with enhanced T cell infiltration into tumors, significantly improving tumor control and survival in multiple cancer xenograft models. Dexamethasone premedication depleted monocytes in circulation and TAMs in tumors, enhanced BsAb-driven T cell infiltration, and anti-tumor response with survival benefit. Conclusion: Reducing TIMs markedly enhanced anti-tumor effects of BsAb-based T cell immunotherapy by improving intratumoral T cell infiltration and persistence. TAM depletion was more effective than PMN- or M-MDSCs depletion at boosting the anti-tumor response of T cell engaging BsAb. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Aberrant methylation of Pax3 gene and neural tube defects in association with exposure to polycyclic aromatic hydrocarbons
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Linlin Wang, Shanshan Lin, Aiguo Ren, Yun Huang, Nicholas D. E. Greene, Zhiwen Li, Lei Jin, and Chloe Santos
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0301 basic medicine ,Male ,lcsh:Medicine ,Pax3 gene ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Pregnancy ,Gene expression ,Vitamin E ,Neural Tube Defects ,Polycyclic Aromatic Hydrocarbons ,Promoter Regions, Genetic ,Genetics (clinical) ,education.field_of_study ,Methylation ,musculoskeletal system ,Polycyclic aromatic hydrocarbon ,Benzo[a]pyrene ,medicine.anatomical_structure ,Benzo(a)pyrene ,CpG site ,Maternal Exposure ,030220 oncology & carcinogenesis ,DNA methylation ,embryonic structures ,Body region ,Female ,Maternal Age ,congenital, hereditary, and neonatal diseases and abnormalities ,lcsh:QH426-470 ,Population ,Biology ,Andrology ,03 medical and health sciences ,Genetics ,medicine ,Animals ,Humans ,education ,Molecular Biology ,PAX3 Transcription Factor ,Research ,lcsh:R ,Neural tube ,DNA Methylation ,lcsh:Genetics ,Disease Models, Animal ,030104 developmental biology ,chemistry ,Gene Expression Regulation ,Case-Control Studies ,CpG Islands ,Developmental Biology - Abstract
Background Neural tube defects (NTDs) are common and severe congenital malformations. Pax3 is an essential gene for neural tube closure in mice but it is unknown whether altered expression or methylation of PAX3 contributes to human NTDs. We examined the potential role of hypermethylation of Pax3 in the development of NTDs by analyzing human NTD cases and a mouse model in which NTDs were induced by benzo[a]pyrene (BaP), a widely studied polycyclic aromatic hydrocarbon (PAH). Methods We extracted methylation information of PAX3 in neural tissues from array data of ten NTD cases and eight non-malformed controls. A validation study was then performed in a larger independent population comprising 73 NTD cases and 29 controls. Finally, we examined methylation patterns and expression of Pax3 in neural tissues from mouse embryos of dams exposed to BaP or BaP and vitamin E. Results Seven CpG sites in PAX3 were hypermethylated in NTD fetuses as compared to controls in the array data. In the validation phase, significantly higher methylation levels in the body region of PAX3 were observed in NTD cases than in controls (P = 0.003). And mean methylation intensity in the body region of PAX3 in fetal neural tissues was positively correlated with median concentrations of PAH in maternal serum. In the mouse model, BaP-induced NTDs were associated with hypermethylation of specific CpG sites within both the promoter and body region of Pax3. Supplementation with vitamin E via chow decreased the rate of NTDs, partly recovered the repressed total antioxidant capacity in mouse embryos exposed to BaP, and this was accompanied by the normalization of Pax3 methylation level and gene expression. Conclusion Hypermethylation of Pax3 may play a role in the development of NTDs; DNA methylation aberration may be caused by exposure to BaP, with possible involvement of oxidative stress. Electronic supplementary material The online version of this article (10.1186/s13148-019-0611-7) contains supplementary material, which is available to authorized users.
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- 2019
7. The feasibility and advantage of uniportal video-assisted thoracoscopic surgery (VATS) in pulmonary lobectomy.
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Linlin Wang, Dabei Liu, Jibin Lu, Suning Zhang, Xueying Yang, Wang, Linlin, Liu, Dabei, Lu, Jibin, Zhang, Suning, and Yang, Xueying
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CHEST endoscopic surgery , *LOBECTOMY (Lung surgery) , *POSTOPERATIVE care , *LYMPH node diseases , *HEMORRHAGE , *THORACIC surgery , *ENDOSCOPIC surgery , *LUNGS , *LUNG tumors , *PNEUMONECTOMY , *POSTOPERATIVE pain , *SURGICAL complications , *MEDICAL drainage - Abstract
Background: Ongoing improvements in technique and instruments for video-assisted thoracoscopic surgery (VATS) have made minimally-invasive uniportal VATS lobectomy a reality. However, the outcomes of the procedure are still under investigation, and at present, uniportal VATS lobectomy is performed infrequently at most hospitals. We have therefore reviewed our outcomes with this procedure in an attempt to validate its safety, efficacy, and feasibility.Methods: We retrospectively analyzed and compared perioperative data for patients who underwent uniportal, two-port, and traditional three-port VATS lobectomy between January 2015 and December 2015 at our hospital.Results: Among 257 patients who had successful VATS lobectomy during the study period, 73 underwent uniportal VATS, 86 underwent two-port VATS, and 98 underwent traditional three-port VATS. There were no surgical or 30-day postoperative mortalities, and no significant differences in operative times, blood loss, number of lymph nodes retrieved and nodal stations explored, drainage times, length of hospital stay, or postoperative complications among the three groups. The visual analogue scale (VAS) pain scores were significantly lower in the uniportal VATS group after surgery (P < 0.05).Conclusions: Uniportal VATS lobectomy is a safe and feasible surgical procedure that is associated with decreased surgical trauma and less postoperative pain compared to traditional VATS. Further long term follow-up analyses in large numbers of patients are ongoing. [ABSTRACT FROM AUTHOR]- Published
- 2017
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8. Study on the expression of PAK4 and P54 protein in breast cancer.
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Yanqing Bi, Mengzi Tian, Jinghong Le, Linlin Wang, Xiaofang Liu, Jianhua Qu, and Min Hao
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P21 gene ,BREAST cancer ,PROTEIN expression ,SERINE/THREONINE kinases ,IMMUNOHISTOCHEMISTRY ,IMMUNOFLUORESCENCE - Abstract
Background: Previous evidence have demonstrated that p21-activated kinase PAK4 was correlated with breast cancer. The aim of this paper is to study the expression and interaction of p21-activated kinase (pAK)-4 and P54 protein in breast cancer. Methods: A total of 80 patients were enrolled in our study (breast fibroma n = 20, breast noninvasive cancer n = 20, early breast invasive cancer n = 20, and advanced breast invasive cancer). The expression of PAK4 was detected by immunohistochemical S-P method, and the relationship between them and the different pathological characteristics were compared. The subcellular localization of P54 and PAK4 in vitro was observed by immunofluorescence assay. Results: The expression of both PAK4 and P54 in breast cancer was much higher than that in breast fibroma. Meanwhile, we found that both PAK4 and P54 increased gradually as breast cancer progressed (advanced invasive > early invasive > noninvasive). The positive staining of P54 were mainly located in the cytoplasm, especially around the nucleus. There was no significant stained region in the cell matrix. The P54 localization in the cytoplasm was verified by confocal experiment, and the PAK4 was co-localized. Conclusions: PAK4 and P54 proteins may be used as molecular markers for diagnosis and treatment of breast cancer. [ABSTRACT FROM AUTHOR]
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- 2016
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9. Gastric tonometry guided therapy in critical care patients: a systematic review and meta-analysis.
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Xin Zhang, Wei Xuan, Ping Yin, Linlin Wang, Xiaodan Wu, and Qingping Wu
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- 2015
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10. Inhibitory receptor immunoglobulin-like transcript 4 was highly expressed in primary ductal and lobular breast cancer and significantly correlated with IL-10.
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Jie Liu, Linlin Wang, Wei Gao, Liwen Li, Xia Cui, Hongyan Yang, Wenli Lin, Qi Dang, Nan Zhang, and Yuping Sun
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DUCTAL carcinoma , *LOBULAR carcinoma , *INTERLEUKIN-10 , *MOLECULES , *CELL lines , *POLYMERASE chain reaction , *WESTERN immunoblotting , *IMMUNOHISTOCHEMISTRY - Abstract
Background Immunoglobulin-like transcript 4 (ILT4) is an inhibitory molecule involved in immune response and has recently been identified to be strongly inducible by IL-10. The aim of the present study was to examine the associations of ILT4 expression with clinicopathological characteristics and IL-10 expression in primary ductal and lobular breast cancer. Methods We studied the expression of ILT4 in 4 cancer cell lines, 117 primary tumor tissues and 97 metastatic lymph nodes from patients with primary ductal and lobular breast cancer by reverse transcription-polymerase chain reaction, western blot or immunohistochemistry analysis. Additionally, IL-10 expression was also investigated using immunohistochemistry in primary tumor tissues. Then the relationship between ILT4 expression and clinicopathological characteristics/IL-10 expression was evaluated. Results ILT4 was over expressed in all 4 human breast cancer cell lines on both mRNA and protein levels. In primary tumor tissues, ILT4 or IL-10 was expressed in the cell membrane, cytoplasm, or both; the positive rate of ILT4 and IL-10 expression was 60.7% (71/117) and 80.34% (94/117), respectively. ILT4 level was significantly correlated with IL-10 (r =0.577; p < 0.01). Furthermore, the expression of ILT4 or IL-10 was associated with less number of Tumor Infiltrating Lymphocytes (TILs) (p = 0.004 and 0.018, respectively) and more lymph node metastasis (p = 0.046 and 0.035, respectively). Conclusion Our data demonstrated the association of ILT4 and IL-10 expression in human breast cancer, suggesting their important roles in immune dysfunction and lymph node metastases. Virtual slides The virtual slides for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1692652692107916. [ABSTRACT FROM AUTHOR]
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- 2014
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